##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780196_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43083 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.384954622472904 34.0 34.0 34.0 34.0 34.0 2 33.507323074066335 34.0 34.0 34.0 34.0 34.0 3 33.56530418030314 34.0 34.0 34.0 34.0 34.0 4 33.621265928556504 34.0 34.0 34.0 34.0 34.0 5 33.59872803658055 34.0 34.0 34.0 34.0 34.0 6 37.18390084255971 38.0 38.0 38.0 36.0 38.0 7 37.16684074925144 38.0 38.0 38.0 36.0 38.0 8 36.99178330199847 38.0 37.0 38.0 35.0 38.0 9 37.07269688740338 38.0 37.0 38.0 36.0 38.0 10-11 36.958649583362345 38.0 37.0 38.0 35.0 38.0 12-13 36.93372095722211 38.0 37.0 38.0 35.0 38.0 14-15 36.83350741591811 38.0 37.0 38.0 34.0 38.0 16-17 36.855047234408005 38.0 37.0 38.0 34.5 38.0 18-19 36.78613374184713 38.0 37.0 38.0 34.0 38.0 20-21 36.853921500359775 38.0 37.0 38.0 34.5 38.0 22-23 36.805781862915765 38.0 37.0 38.0 34.0 38.0 24-25 36.83009539725646 38.0 37.0 38.0 34.5 38.0 26-27 36.61820207506442 38.0 37.0 38.0 34.0 38.0 28-29 36.57111853863473 38.0 37.0 38.0 34.0 38.0 30-31 36.59632569691061 38.0 37.0 38.0 34.0 38.0 32-33 36.24149896711 38.0 37.0 38.0 33.5 38.0 34-35 36.4700694009238 38.0 37.0 38.0 34.0 38.0 36-37 36.42659517675185 38.0 37.0 38.0 34.0 38.0 38-39 35.392022375414896 38.0 36.5 38.0 31.0 38.0 40-41 35.907121138267996 38.0 37.0 38.0 32.5 38.0 42-43 36.303820532460605 38.0 37.0 38.0 34.0 38.0 44-45 36.412088294686995 38.0 37.0 38.0 34.0 38.0 46-47 36.30756911078616 38.0 37.0 38.0 34.0 38.0 48-49 36.35999117981571 38.0 37.0 38.0 34.0 38.0 50-51 36.02082027713948 38.0 37.0 38.0 32.5 38.0 52-53 36.11212079010282 38.0 37.0 38.0 33.5 38.0 54-55 36.010526193626255 38.0 37.0 38.0 33.0 38.0 56-57 36.082468723162265 38.0 37.0 38.0 33.5 38.0 58-59 35.90505535826196 38.0 37.0 38.0 32.5 38.0 60-61 35.90998769816401 38.0 37.0 38.0 32.0 38.0 62-63 35.91371306547826 38.0 37.0 38.0 32.0 38.0 64-65 35.75678341805353 38.0 36.5 38.0 31.5 38.0 66-67 35.125884919805955 38.0 36.0 38.0 27.5 38.0 68-69 34.9981431190957 38.0 36.0 38.0 27.0 38.0 70-71 34.246001903302925 38.0 35.0 38.0 23.5 38.0 72-73 33.15138221572314 38.0 34.0 38.0 15.0 38.0 74-75 32.8352714527772 38.0 33.5 38.0 11.0 38.0 76 31.976278346447554 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1407663171533855 1101 2 -0.025685462385823143 1101 3 0.2709939605388385 1101 4 0.08242276406751614 1101 5 0.10459524620763005 1101 6 0.11375005375288794 1101 7 0.2131214469854612 1101 8 0.43329396817724586 1101 9 -0.035377883673596955 1101 10-11 -0.14774273511577718 1101 12-13 0.09016558694142418 1101 14-15 0.26146517647813994 1101 16-17 0.44860606233746125 1101 18-19 0.21179656102573574 1101 20-21 0.38899620401506496 1101 22-23 -0.2724585426011359 1101 24-25 0.10104256879620976 1101 26-27 -0.3628957132762167 1101 28-29 -0.20372351926647525 1101 30-31 0.48692517389680035 1101 32-33 0.2989512325307544 1101 34-35 -0.07442737491594897 1101 36-37 0.37577299888508975 1101 38-39 -0.5286849675575382 1101 40-41 -0.03840850791647199 1101 42-43 0.2365346906203314 1101 44-45 0.39293139111835274 1101 46-47 0.19363998937822657 1101 48-49 0.3629063281133966 1101 50-51 0.12428409200559543 1101 52-53 -0.0427181475681806 1101 54-55 0.6713375083666193 1101 56-57 0.2747243541698978 1101 58-59 0.6192646991118451 1101 60-61 -0.1513272443211875 1101 62-63 0.3515670183628643 1101 64-65 -0.13344266477358246 1101 66-67 0.21933951941868202 1101 68-69 0.09941389907045561 1101 70-71 0.07077563982819157 1101 72-73 0.5820140694077622 1101 74-75 0.13127551483815836 1101 76 -1.2295289767134108 1102 1 0.4163287613786437 1102 2 0.262605364724223 1102 3 -0.07534956489444511 1102 4 0.07471997885311055 1102 5 0.3632021693206866 1102 6 0.45685277321181417 1102 7 0.1314437427911983 1102 8 0.350676899932445 1102 9 0.538432646597407 1102 10-11 -0.02273686409045439 1102 12-13 0.18074376551452787 1102 14-15 0.4353414622326852 1102 16-17 0.5378926153410717 1102 18-19 0.3998102757408759 1102 20-21 0.3836653130282599 1102 22-23 0.21502443142544791 1102 24-25 0.29232057358697006 1102 26-27 0.47191711843670703 1102 28-29 0.46745209484276984 1102 30-31 0.7371717569600662 1102 32-33 0.8237504434649523 1102 34-35 0.7462010596338544 1102 36-37 0.6708507312602663 1102 38-39 0.5580653025096254 1102 40-41 0.5722016090343516 1102 42-43 0.43286177718272967 1102 44-45 0.684204699088852 1102 46-47 0.7220557519069928 1102 48-49 0.8788050646687395 1102 50-51 0.5545832794556844 1102 52-53 0.6266726748450466 1102 54-55 0.8767059739154419 1102 56-57 0.4981286094890507 1102 58-59 0.9854053219102141 1102 60-61 0.9410357259479412 1102 62-63 0.6047256299828021 1102 64-65 0.7391326017709687 1102 66-67 0.8928400595530093 1102 68-69 1.0676399100610112 1102 70-71 0.864186001013671 1102 72-73 0.4100399958555556 1102 74-75 0.43076825825087184 1102 76 0.6058005052273145 1103 1 -0.09953947634389948 1103 2 0.29373979320964594 1103 3 0.0840638693534288 1103 4 -0.20531786409863884 1103 5 -0.019011643356705576 1103 6 0.1712244942060508 1103 7 0.30186603573005044 1103 8 0.19616584807040027 1103 9 0.312278006535486 1103 10-11 0.14179396931454136 1103 12-13 0.3968571223563657 1103 14-15 0.206477610852275 1103 16-17 0.2552745711336257 1103 18-19 0.3514530038736652 1103 20-21 0.1422659838805842 1103 22-23 0.48723566390157913 1103 24-25 -0.08208813029192896 1103 26-27 0.144726097537081 1103 28-29 0.44401526677017955 1103 30-31 0.3065576690612133 1103 32-33 -0.639567695349875 1103 34-35 -0.03553541581122488 1103 36-37 0.3574207837243577 1103 38-39 -0.37654809733556505 1103 40-41 -0.5305334042966905 1103 42-43 -0.1268483802095517 1103 44-45 0.1476518479664648 1103 46-47 -0.5103988795542591 1103 48-49 0.3244058215277761 1103 50-51 0.001022601722823424 1103 52-53 0.12807028385854835 1103 54-55 -0.4418843761318598 1103 56-57 -0.5440791827187397 1103 58-59 0.04928680466586144 1103 60-61 0.4050419184309604 1103 62-63 0.02815815771996455 1103 64-65 0.05413217173742879 1103 66-67 -0.9146984284490571 1103 68-69 -0.5582954128941822 1103 70-71 -0.31236426990746224 1103 72-73 -0.13752171395780266 1103 74-75 -0.9217087349545992 1103 76 -0.4861836695383168 1104 1 -0.09105123256153291 1104 2 0.29221190932882024 1104 3 0.2778080336858224 1104 4 0.20271201451947007 1104 5 0.17426566756766704 1104 6 0.18022203261535452 1104 7 0.17390576071095154 1104 8 -0.3390603636264018 1104 9 0.13177550250357228 1104 10-11 0.12906160364100572 1104 12-13 0.15364771738224903 1104 14-15 0.1837291175155471 1104 16-17 -0.06460489580466344 1104 18-19 0.2671435225053571 1104 20-21 0.19117948867644685 1104 22-23 -0.09577936028991019 1104 24-25 0.31450383982944885 1104 26-27 0.3493139933014504 1104 28-29 0.2088060315609468 1104 30-31 0.13885119253119882 1104 32-33 0.6119814091404052 1104 34-35 0.42751950312604237 1104 36-37 0.2807931629790943 1104 38-39 0.21196306470501014 1104 40-41 0.030497068498490876 1104 42-43 -0.23683479901949056 1104 44-45 0.11501455062328603 1104 46-47 -0.014536898398162634 1104 48-49 0.19415372068743864 1104 50-51 0.4979456133517175 1104 52-53 -0.15452457226571426 1104 54-55 0.20867584795777816 1104 56-57 0.1909390670053952 1104 58-59 0.18622339748480954 1104 60-61 0.35805948909558794 1104 62-63 0.6546542106866085 1104 64-65 0.7489798077615362 1104 66-67 0.07972055126403887 1104 68-69 -0.14867398856686975 1104 70-71 0.013690394382500415 1104 72-73 0.14500948033808925 1104 74-75 0.1539427377556919 1104 76 0.9493056777157349 1105 1 -0.2553836321880496 1105 2 -0.0031062179220811004 1105 3 -0.0826027973132426 1105 4 -0.0012362314455813816 1105 5 -0.025814364445139404 1105 6 -0.21900432459419505 1105 7 -0.20184453940910174 1105 8 0.14700072747670845 1105 9 0.02439921865669703 1105 10-11 -0.0189976200484665 1105 12-13 -0.10298827034616664 1105 14-15 0.24822158116767667 1105 16-17 -0.4052078036344682 1105 18-19 -0.07912831956479494 1105 20-21 0.07346573650890775 1105 22-23 0.2531602155404187 1105 24-25 -0.04977520512186828 1105 26-27 -0.5585824986286525 1105 28-29 -0.04856358295153029 1105 30-31 -0.1057799932764496 1105 32-33 0.016276460494275113 1105 34-35 -0.05980876151314618 1105 36-37 -0.2178729701408244 1105 38-39 -0.8065109915841688 1105 40-41 -0.2525494834556241 1105 42-43 -0.11525283289971355 1105 44-45 -0.2908144476426884 1105 46-47 -0.050751384192480486 1105 48-49 -0.44090030092120003 1105 50-51 -0.42275600062720287 1105 52-53 -0.2335561849107748 1105 54-55 -0.22589798157403607 1105 56-57 0.05255037819653552 1105 58-59 -0.2319438694219471 1105 60-61 -0.05290489665871689 1105 62-63 -0.13526175569995047 1105 64-65 -0.5568758678420451 1105 66-67 0.5888575393926203 1105 68-69 0.24921850176259142 1105 70-71 -0.23954423280171255 1105 72-73 -0.33418219633257706 1105 74-75 -0.28209834534421674 1105 76 0.4689802141969963 1106 1 0.11182633904876127 1106 2 0.1113986444255417 1106 3 -0.1561087097453253 1106 4 -0.15066781616000213 1106 5 0.12768059058385006 1106 6 0.11674876858938177 1106 7 -0.2343058460794154 1106 8 -0.01489490325880638 1106 9 0.1439262240897463 1106 10-11 0.1838056489101163 1106 12-13 0.10118467399934872 1106 14-15 0.2876682323113542 1106 16-17 0.24716858316186574 1106 18-19 0.22395801564042728 1106 20-21 0.0444057903828039 1106 22-23 0.09793033835464371 1106 24-25 0.46081695120375343 1106 26-27 0.1615699918977498 1106 28-29 -0.01192476635105777 1106 30-31 0.11665528956151405 1106 32-33 0.19221126394585752 1106 34-35 0.19424319483986352 1106 36-37 -0.22056666506845346 1106 38-39 -0.09660823853664624 1106 40-41 0.29287808354432343 1106 42-43 0.08697973798098246 1106 44-45 0.19149391194275722 1106 46-47 0.2905032384619517 1106 48-49 -0.015479125739716437 1106 50-51 0.43488907082419814 1106 52-53 0.311391995703751 1106 54-55 0.22038791533506696 1106 56-57 0.2766520994219306 1106 58-59 -0.24764948052545321 1106 60-61 0.754427432245997 1106 62-63 0.4292956108708452 1106 64-65 0.427007618438374 1106 66-67 0.143895662024228 1106 68-69 -0.055876690676797125 1106 70-71 -0.2389963626469367 1106 72-73 -0.22721054861308687 1106 74-75 -0.9393370797641509 1106 76 -0.3106846718916678 1107 1 0.06891250962633677 1107 2 0.11874690288829015 1107 3 0.054345412627668566 1107 4 0.22742457594306842 1107 5 -0.33496576223053154 1107 6 0.02817775991844229 1107 7 0.23745234722876774 1107 8 0.20256455884867108 1107 9 0.440110028528089 1107 10-11 0.08984591736649605 1107 12-13 0.15534133886978196 1107 14-15 0.537362054875409 1107 16-17 0.393913304662874 1107 18-19 -0.4937218942209398 1107 20-21 0.14755084500486504 1107 22-23 0.18123509512729186 1107 24-25 0.13715066011474164 1107 26-27 0.04715760763282617 1107 28-29 0.23029832677586626 1107 30-31 0.47294784822511104 1107 32-33 0.5559010694619531 1107 34-35 0.3725364393409194 1107 36-37 -0.42220287728616057 1107 38-39 0.3148207527924072 1107 40-41 0.14275316315045927 1107 42-43 -0.08359783513418506 1107 44-45 -0.004827059986894255 1107 46-47 0.6117690984531379 1107 48-49 0.5814444033838768 1107 50-51 0.2820208006042293 1107 52-53 0.025667212477372914 1107 54-55 0.157437834504222 1107 56-57 0.12951547080292158 1107 58-59 0.29871803037446654 1107 60-61 -0.1621208458120975 1107 62-63 0.09032044798444616 1107 64-65 0.5307650279323255 1107 66-67 0.7895626389506987 1107 68-69 1.1345901686775335 1107 70-71 0.11879034190408788 1107 72-73 -0.04362655801885751 1107 74-75 0.37222907905620417 1107 76 -0.1744479599923885 1108 1 0.23812286131843763 1108 2 0.2093242087940581 1108 3 0.21393399922355627 1108 4 0.21459555581478185 1108 5 -0.19650082084588405 1108 6 -0.1296413066597495 1108 7 0.09948508334909434 1108 8 0.28274593465048525 1108 9 0.07310051735799306 1108 10-11 0.12393682645740256 1108 12-13 -0.32392209842285524 1108 14-15 0.04305769743236709 1108 16-17 0.06804513390419231 1108 18-19 0.03110668352734791 1108 20-21 0.43501490162950773 1108 22-23 0.21126163792755648 1108 24-25 0.48176468356088264 1108 26-27 0.29732350013448183 1108 28-29 0.4954221931771059 1108 30-31 0.7800425175460575 1108 32-33 0.047661741879558406 1108 34-35 0.12463774436193376 1108 36-37 0.30493160623517923 1108 38-39 -0.740725586513058 1108 40-41 -0.05380180256508993 1108 42-43 0.15561915225798373 1108 44-45 0.17524925056386564 1108 46-47 0.327263458108078 1108 48-49 0.29843179555375343 1108 50-51 0.2407412164414353 1108 52-53 0.2703863011745682 1108 54-55 0.18906800482052688 1108 56-57 0.3579770943375351 1108 58-59 0.16238204276110224 1108 60-61 0.45320209159113034 1108 62-63 0.7878984174602195 1108 64-65 0.7348681024733565 1108 66-67 0.5982885845379542 1108 68-69 1.0234145438157753 1108 70-71 0.46036300281981113 1108 72-73 -0.5693398957759896 1108 74-75 -1.3107779990238626 1108 76 -0.7448416781963267 1109 1 0.23079148301932406 1109 2 -0.19023590850799366 1109 3 -0.0587932735037171 1109 4 0.17256232023315476 1109 5 0.05472107553730865 1109 6 -0.6570117856431352 1109 7 0.3233365007489013 1109 8 -0.357772149415986 1109 9 0.06625789761215373 1109 10-11 0.03742656252779142 1109 12-13 0.07563801887919652 1109 14-15 0.3724809623395018 1109 16-17 0.019413657853355915 1109 18-19 0.11370687903076515 1109 20-21 0.09801587914660814 1109 22-23 -0.343723699315845 1109 24-25 0.041759795975352176 1109 26-27 -0.9717380834432277 1109 28-29 -0.5971975526684474 1109 30-31 -0.24806109926723252 1109 32-33 -0.7677097146209277 1109 34-35 -0.4555186583751123 1109 36-37 -0.38226291966902437 1109 38-39 -1.5034333088981953 1109 40-41 -0.6665828387332198 1109 42-43 -0.21424888293263677 1109 44-45 -0.6531964972367206 1109 46-47 -0.36518522224382366 1109 48-49 -0.0897646853846652 1109 50-51 -0.87497237104634 1109 52-53 -0.3123360839671676 1109 54-55 -0.5501793070074399 1109 56-57 -0.46154880986578206 1109 58-59 -0.4745925417941308 1109 60-61 -0.5114851029347705 1109 62-63 -0.17251213972452462 1109 64-65 0.0650245052104097 1109 66-67 -0.9398345826174648 1109 68-69 -0.7827926898108117 1109 70-71 -0.8196956482065829 1109 72-73 -2.3809234734886005 1109 74-75 -1.7988034144686367 1109 76 -1.5837957348923197 1110 1 -0.07324077504519266 1110 2 0.04417269364254395 1110 3 0.1912067264962829 1110 4 -0.058889292670066595 1110 5 0.2928931185480579 1110 6 -0.21373221575066026 1110 7 0.22978811365212692 1110 8 -0.5308904289858773 1110 9 0.07916112341860071 1110 10-11 -0.04348741596683681 1110 12-13 0.27456275006199604 1110 14-15 -0.3671695752949091 1110 16-17 0.0843330126920705 1110 18-19 0.18413698655765387 1110 20-21 0.19183308344768335 1110 22-23 -0.24479896813305402 1110 24-25 -0.06899289219669669 1110 26-27 0.2802780455890286 1110 28-29 0.5101949204498339 1110 30-31 0.515648578152117 1110 32-33 0.5984193176371306 1110 34-35 0.021228653452844526 1110 36-37 0.2564467577503322 1110 38-39 -0.36458922287668827 1110 40-41 0.344304258040971 1110 42-43 0.29274036437919904 1110 44-45 0.32865834147295914 1110 46-47 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12-13 -0.4381565481836205 2116 14-15 -0.19458185599666677 2116 16-17 -1.0400090925710899 2116 18-19 -0.26235424151252573 2116 20-21 -0.5849213025172233 2116 22-23 0.11616594414765302 2116 24-25 -0.043554295705469315 2116 26-27 0.08619909822061089 2116 28-29 0.07576509588842839 2116 30-31 0.10380647288896228 2116 32-33 0.018156159742396483 2116 34-35 0.090746197312491 2116 36-37 0.10118359985559522 2116 38-39 -0.3134927316486156 2116 40-41 -0.9734588998537674 2116 42-43 -0.16734735491904473 2116 44-45 -1.0891048164217807 2116 46-47 -0.7983346280163204 2116 48-49 -1.0759701183218482 2116 50-51 -0.5808297564453255 2116 52-53 -0.28623251860214793 2116 54-55 0.061875022364382914 2116 56-57 -0.41634507472146964 2116 58-59 -0.40201189663283543 2116 60-61 -0.41322534062577887 2116 62-63 -0.7665274198603456 2116 64-65 -0.18518771889825558 2116 66-67 -0.6726444298161098 2116 68-69 -0.7106364928668398 2116 70-71 0.268377356886802 2116 72-73 0.023363453506306087 2116 74-75 0.010194887738499858 2116 76 -0.35289590467160536 2117 1 -0.2225650993695183 2117 2 -0.29681829734844456 2117 3 0.049314834604388125 2117 4 0.2309755721947937 2117 5 -0.09044021478527497 2117 6 -0.002696179714625657 2117 7 0.2176259514899641 2117 8 -1.2115210596165085 2117 9 0.19943139368887586 2117 10-11 -0.3020459745253916 2117 12-13 0.3376258131250509 2117 14-15 0.4437538481285159 2117 16-17 -0.1004642846052306 2117 18-19 -0.01270986028919907 2117 20-21 0.09768893930354494 2117 22-23 0.12096679763271823 2117 24-25 -1.3755244237282298 2117 26-27 -0.6977624949515189 2117 28-29 -1.305458232703316 2117 30-31 -0.40597304346950125 2117 32-33 -0.85631041209259 2117 34-35 -0.23428936456517846 2117 36-37 -0.18206675576318077 2117 38-39 0.5204332996458376 2117 40-41 0.09126371750043205 2117 42-43 -0.02189927526043789 2117 44-45 -0.06154436122974971 2117 46-47 -0.05295767211305247 2117 48-49 -0.9902659931440354 2117 50-51 -0.06198196128173805 2117 52-53 0.047515703303972145 2117 54-55 -0.49786893211641825 2117 56-57 -0.5559254445649984 2117 58-59 -0.4830929777139161 2117 60-61 0.012272525661565226 2117 62-63 -0.13654875698694724 2117 64-65 -0.13593451832926462 2117 66-67 -0.6283698921205172 2117 68-69 -0.7168598214585842 2117 70-71 -0.11764682519001468 2117 72-73 0.6014217749856812 2117 74-75 1.4698321565862926 2117 76 0.951513768159117 2118 1 0.27917857979351623 2118 2 -0.05313913431444206 2118 3 -0.31685778963274913 2118 4 0.027401029910578245 2118 5 -0.348504730914307 2118 6 0.13331079500202492 2118 7 0.13785262978115753 2118 8 -0.12390118167405006 2118 9 -0.16761307012978222 2118 10-11 -0.1198315019883438 2118 12-13 -0.4646845617659707 2118 14-15 -0.49848677959598575 2118 16-17 -0.19832827763050886 2118 18-19 0.3798359112888292 2118 20-21 -0.38988734665984026 2118 22-23 0.3014375910067315 2118 24-25 0.1748243121043842 2118 26-27 0.2621328842987083 2118 28-29 0.8503830924928479 2118 30-31 -0.05048045410594426 2118 32-33 -0.3765806823628637 2118 34-35 -0.1813251099880162 2118 36-37 -0.184682274507729 2118 38-39 1.1618892717469649 2118 40-41 -0.43271187099128383 2118 42-43 0.3139769235189789 2118 44-45 0.11696479308586305 2118 46-47 0.8243309067884326 2118 48-49 0.38200950816371915 2118 50-51 0.8105114999214038 2118 52-53 0.5747606902263698 2118 54-55 -0.3808244682977602 2118 56-57 -0.29219397115610946 2118 58-59 0.20282681304458805 2118 60-61 -0.39051736099929 2118 62-63 -0.15638310746646056 2118 64-65 -0.2978787205960529 2118 66-67 0.05210090125349609 2118 68-69 -0.5852120446495235 2118 70-71 -0.835824680464647 2118 72-73 1.223915236188823 2118 74-75 1.205228843595883 2118 76 0.4000752328496162 2119 1 0.21579431267410598 2119 2 0.15908497434429592 2119 3 0.1429611124611938 2119 4 -0.31809981898585704 2119 5 -0.6957033726800148 2119 6 -0.02996028592610145 2119 7 -0.5938960968741895 2119 8 -0.4580834114420185 2119 9 -1.3515406309673637 2119 10-11 -0.14699618789666147 2119 12-13 -0.17280567099063404 2119 14-15 -1.2077397507335021 2119 16-17 -0.18913189958863086 2119 18-19 0.3560668111190566 2119 20-21 0.43042957467575604 2119 22-23 -0.4430445821681417 2119 24-25 -0.7957472781616062 2119 26-27 -0.3063447614285195 2119 28-29 -0.22686648305894153 2119 30-31 -0.4203163341285787 2119 32-33 0.1892087913213416 2119 34-35 -0.5320608202313721 2119 36-37 -0.567676049267213 2119 38-39 0.0987879701057679 2119 40-41 0.22829548611115058 2119 42-43 -6.806882523875402E-4 2119 44-45 -0.04305218484282847 2119 46-47 -0.2983346280163204 2119 48-49 -0.7996543288481632 2119 50-51 -0.5983736160944488 2119 52-53 -0.6700044484267096 2119 54-55 0.14520835569771862 2119 56-57 0.287602293699571 2119 58-59 -0.016046984352136917 2119 60-61 -0.5294534108012172 2119 62-63 -1.062580051439305 2119 64-65 -0.7136964908280774 2119 66-67 -1.192381271921377 2119 68-69 -0.581250527954559 2119 70-71 0.2837282340797813 2119 72-73 0.49485468157647716 2119 74-75 0.9597562912472668 2119 76 -0.9055274836189753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 2.0 22 5.0 23 8.0 24 25.0 25 46.0 26 96.0 27 177.0 28 292.0 29 483.0 30 689.0 31 1055.0 32 1691.0 33 2788.0 34 4044.0 35 5351.0 36 7340.0 37 18989.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.80491144999188 17.108836432003343 13.077083768539795 35.009168349464986 2 18.364552143536894 20.674047768261264 36.00724183552678 24.954158252675068 3 18.39240535710141 18.49917600909872 32.96659935473388 30.14181927906599 4 14.11461597381798 25.65977299630945 31.875681823457047 28.349929206415524 5 14.848083931016875 30.640856022096884 40.062205510294085 14.44885453659216 6 28.01104844138059 36.984425411415174 16.33590975558805 18.668616391616183 7 26.21683726759975 25.47408490587935 23.02300211220203 25.286075714318873 8 23.087992943852566 36.31130608360606 15.755634472993988 24.845066499547386 9 21.59320381589026 15.795093192210386 16.981175869832647 45.63052712206671 10-11 26.474479493071513 26.92941531462526 23.635772810621358 22.96033238168187 12-13 25.209479377016457 18.409813615579232 25.6156720748323 30.76503493257201 14-15 21.899589165099922 18.174221850846042 19.97191467632245 39.95427430773159 16-17 27.111621753359792 25.95687394099761 19.033029269085254 27.89847503655734 18-19 29.997911008982665 24.240419655084374 19.355662326207554 26.406007009725414 20-21 24.90309402780679 22.229185525613353 19.570364180767356 33.2973562658125 22-23 22.771162639556206 26.837731819975396 19.380033888076504 31.011071652391898 24-25 27.356497922614487 25.23152983775503 22.825708516120045 24.58626372351043 26-27 29.799456862335493 26.13559872803658 20.004410092147715 24.06053431748021 28-29 25.170020657800062 25.767704198871943 20.72627254369473 28.336002599633265 30-31 23.96072696887403 23.64621776570805 28.940649444096277 23.452405821321634 32-33 26.80987860641088 20.157602766752547 25.410254624794003 27.622264002042566 34-35 27.29150709096395 19.686419237286167 22.540213077083767 30.48186059466611 36-37 31.34995241742683 23.078708539331057 22.245433233525986 23.32590580971613 38-39 22.88605714550983 24.392451779124016 26.985121741754288 25.736369333611865 40-41 23.487222338277277 19.35798342733793 27.390153889004942 29.76464034537985 42-43 27.93097045238261 19.125873314300303 26.43966297611587 26.503493257201217 44-45 21.78585520971149 24.01179119374231 29.75535594085834 24.44699765568786 46-47 25.23965369171135 20.634589049044866 28.453218206717267 25.672539052526517 48-49 25.197873871364575 19.324327460947472 24.05008936239352 31.42770930529443 50-51 23.990901283568924 20.090290833971636 26.887635494278484 29.031172388180952 52-53 19.63071281015714 19.80247429380498 36.01072348722234 24.55608940881554 54-55 21.222988185595245 21.626859782280714 29.496553164821393 27.653598867302648 56-57 23.879488429310864 23.63461226005617 24.789360072418358 27.69653923821461 58-59 20.431492700136943 24.27871782373558 30.13601652624005 25.153772949887426 60-61 23.96304807000441 28.156117262029106 24.79748392637467 23.08335074159181 62-63 19.930134855975677 35.76816841909802 21.03033679177402 23.27135993315229 64-65 19.053919179258642 37.62969152565977 20.221433047837895 23.094956247243694 66-67 19.373070584685376 36.24399415082515 20.083327530580508 24.299607733908967 68-69 18.626836571269408 36.44709049973308 20.72163034143398 24.20444258756354 70-71 19.064364134345333 34.57396188751944 21.398231320938656 24.963442657196573 72-73 19.477520135552307 31.459044170554513 22.065547895921824 26.99788779797136 74-75 19.997446788756587 30.33447067288722 22.306942413480954 27.361140124875238 76 19.868625676020706 30.63853492096651 21.7649652995381 27.727874103474687 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 1.0 18 2.5 19 2.5 20 5.0 21 9.0 22 13.0 23 25.5 24 30.0 25 26.0 26 53.5 27 83.5 28 116.0 29 146.0 30 186.5 31 259.5 32 350.0 33 408.0 34 474.5 35 614.0 36 716.5 37 746.0 38 891.0 39 1153.5 40 1487.5 41 2313.5 42 2923.0 43 3330.0 44 3686.0 45 3340.5 46 3046.0 47 2669.5 48 2097.0 49 1723.5 50 1546.0 51 1454.5 52 1184.5 53 920.5 54 835.0 55 862.0 56 813.0 57 740.0 58 743.0 59 742.5 60 747.5 61 812.0 62 871.0 63 934.0 64 1032.0 65 1064.0 66 1086.5 67 1112.0 68 1092.5 69 1026.5 70 911.5 71 843.0 72 762.0 73 572.5 74 372.0 75 280.0 76 197.0 77 85.5 78 35.0 79 13.0 80 7.0 81 1.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 43083.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.04530789406495 #Duplication Level Percentage of deduplicated Percentage of total 1 73.62296032469146 41.26221479469861 2 15.600927689886523 17.48717591625467 3 5.284519175018636 8.885175127080288 4 2.277809989232171 5.106422486827751 5 1.271432121262321 3.5628902351275444 6 0.6584941605234822 2.214330478378943 7 0.4058643253540959 1.592275375438108 8 0.2360639443386068 1.0584221154515703 9 0.14909301747701484 0.7520367662419052 >10 0.44313757972334966 3.7694682357310305 >50 0.016565890830779424 0.6150917995497064 >100 0.020707363538474282 2.6251653784555393 >500 0.004141472707694856 1.4762203189192953 >1k 0.008282945415389712 9.593110971845045 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 2621 6.083606062716153 No Hit CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG 1512 3.509504909128891 RNA PCR Primer, Index 18 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 636 1.4762203189192953 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 417 0.9678991713668964 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 273 0.6336606085927163 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 193 0.44797251816261635 RNA PCR Primer, Index 30 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT 128 0.29710094468816006 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT 120 0.27853213564515006 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 77 0.1787247870389713 No Hit CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 65 0.1508715734744563 No Hit CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 63 0.14622937121370377 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT 60 0.13926606782257503 No Hit CACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGTCTTCTG 46 0.10677065199730754 RNA PCR Primer, Index 30 (95% over 24bp) CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC 45 0.10444955086693128 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009284404521505002 0.0 2 0.0 0.0 0.0 0.009284404521505002 0.0 3 0.0 0.0 0.0 0.009284404521505002 0.0 4 0.0 0.0 0.0 0.011605505651881252 0.0 5 0.0 0.0 0.0 0.016247707912633753 0.0 6 0.0 0.0 0.0 0.016247707912633753 0.0 7 0.0 0.0 0.0 0.016247707912633753 0.0 8 0.0 0.0 0.0 0.016247707912633753 0.0 9 0.0 0.0 0.0 0.016247707912633753 0.0 10 0.0 0.0 0.0 0.020889910173386254 0.0 11 0.0 0.0 0.0 0.023211011303762505 0.0 12 0.0 0.0 0.0 0.023211011303762505 0.0 13 0.0 0.0 0.0 0.023211011303762505 0.0 14 0.0 0.0 0.0 0.023211011303762505 0.0 15 0.0 0.0 0.0 0.023211011303762505 0.0 16 0.0 0.0 0.0 0.023211011303762505 0.0 17 0.0 0.0 0.0 0.023211011303762505 0.0 18 0.0 0.0 0.0 0.023211011303762505 0.0 19 0.0 0.0 0.0 0.030174314694891256 0.0 20 0.0 0.0 0.0 0.03249541582526751 0.0 21 0.0 0.0 0.0 0.03481651695564376 0.0 22 0.0 0.0 0.0 0.03713761808602001 0.0 23 0.0 0.0 0.0 0.03945871921639626 0.0 24 0.0 0.0 0.0 0.04410092147714876 0.0 25 0.0 0.0 0.0 0.04874312373790126 0.0 26 0.0 0.0 0.0 0.06034862938978251 0.0 27 0.0 0.0 0.0 0.10212844973655502 0.0 28 0.0 0.0 0.0 0.15319267460483255 0.0 29 0.0 0.0 0.0 0.20425689947311004 0.0 30 0.0 0.0 0.0 0.27853213564515006 0.0 31 0.0 0.0 0.0 0.3574495740779426 0.0 32 0.0 0.0 0.0 0.43404591138035886 0.0 33 0.0 0.0 0.0 0.5709908780725577 0.0 34 0.0 0.0 0.0 0.7891743843279252 0.0 35 0.0 0.0 0.0 0.9864679804099065 0.0 36 0.0 0.0 0.0 1.3601652624004827 0.0 37 0.0 0.0 0.0 1.8615231065617528 0.0 38 0.0 0.0 0.0 2.3187800292458745 0.0 39 0.0 0.0 0.0 2.7853213564515005 0.0 40 0.0 0.0 0.0 3.2124039644407305 0.0 41 0.0 0.0 0.0 3.762504932339902 0.0 42 0.0 0.0 0.0 4.386881136411113 0.0 43 0.0 0.0 0.0 5.011257340482325 0.0 44 0.0 0.0 0.0 5.563679409511872 0.0 45 0.0 0.0 0.0 6.111459276280668 0.0 46 0.0 0.0 0.0 6.684771255483602 0.0 47 0.0 0.0 0.0 7.232551122252397 0.0 48 0.0 0.0 0.0 7.680523640415013 0.0 49 0.0 0.0 0.0 8.123853956316877 0.0 50 0.0 0.0 0.0 8.555578766566859 0.0 51 0.0 0.0 0.0 9.061578812988882 0.0 52 0.0 0.0 0.0 9.743982545319499 0.0 53 0.0 0.0 0.0 10.533156929647424 0.0 54 0.0 0.0 0.0 11.171459740500893 0.0 55 0.0 0.0 0.0 11.714597405008936 0.0 56 0.0 0.0 0.0 12.199707541257572 0.0 57 0.0 0.0 0.0 12.638395654898684 0.0 58 0.0 0.0 0.0 12.988881925585497 0.0 59 0.0 0.0 0.0 13.31615718496855 0.0 60 0.0 0.0 0.0 13.794304017826057 0.0 61 0.0 0.0 0.0 14.184249007729267 0.0 62 0.0 0.0 0.0 14.660074739456398 0.0 63 0.0 0.0 0.0 15.170716988139173 0.0 64 0.0 0.0 0.0 15.727781259429474 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 15 0.0022103465 70.0 1 TCTTTAT 35 2.9087278E-7 60.000004 1 TTATCCC 20 0.0069068973 52.5 53 AAGTTAA 20 0.0069068973 52.5 19 GCTTATA 20 0.0069068973 52.5 3 TCAGAGC 20 0.0069068973 52.5 8 GTCAACC 20 0.0069068973 52.5 20 GCTCGTA 20 0.0069068973 52.5 38 CGATCGG 20 0.0069068973 52.5 26 CTTTATA 45 1.6480135E-6 46.666664 2 TTTATAC 50 3.4032018E-6 42.000004 3 CGGAACT 55 6.550319E-6 38.181816 34 GCCGGAA 70 8.444258E-7 35.000004 32 CCGGAAC 60 1.1896227E-5 35.0 33 AACTCGT 60 1.1896227E-5 35.0 37 ACTCGTA 65 2.0576521E-5 32.307693 38 GGAACTC 80 2.3965877E-6 30.625 35 CTCGTAT 755 0.0 28.278145 39 CTTGAAA 725 0.0 27.517242 57 CGTATGC 790 0.0 27.468355 41 >>END_MODULE