FastQCFastQC Report
Wed 25 May 2016
SRR1780195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385832
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAAG250.002360247835.1712722
TTACCAG250.002363251835.1621555
ATTTGCG401.823814E-532.9816230
ACAATGC401.8302979E-532.964528
ATTGGCC300.005763173629.30939542
TATCATG300.005770480729.30185
TACGTCG300.005770480729.30185
ATACAAT300.005770480729.30186
GTATTAT655.955917E-727.0548251
TTTTTCG450.00138522424.47844119
GGGAATA450.001403147824.42449631
CAGTAAT450.001403147824.4244961
TAATACA551.6025256E-423.97424
GAAAATT602.8786922E-421.98774726
TATTATA904.09902E-721.9820462
GTCCAGT703.2283184E-521.9820461
CAATGCC500.002593430721.9763479
CTGGCGT500.002593430721.9763474
CCAGTCA550.00446236720.01221714
AATTTGC550.004492631619.98886129
Produced by FastQC (version 0.11.5)