FastQCFastQC Report
Wed 25 May 2016
SRR1780195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385832
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT145483.770552986792179No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC40221.0424226088038318No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG38891.0079516473491055No Hit
CACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTG33530.8690310808849447TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC9690.2511455763130067No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT4390.11378009081672853TruSeq Adapter, Index 5 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA1000.037.4029772
TACTGAC301.2998757E-436.6648334
TCTTCTG4000.035.7482144
GTCTTCT4150.035.51629643
ATGCCGT22000.031.69841444
TTCTGAT353.2147768E-431.42719
CGTCTTC4700.030.8920742
TCTTTAT1300.030.515381
TTAATAC300.00573799629.3356672
GCTTATA300.00573799629.3356673
TGTGTAC300.00573799629.3356672
AACCGGT300.005741632529.3318657
TTAAAGC300.005741632529.33186528
ATCGGTT300.005741632529.33186522
TAAATGT300.005741632529.33186525
AAATGTC300.005741632529.33186526
TTTATAC1450.028.8298763
CCGTCTT5050.028.31541441
ACAATTC407.0254545E-427.49862321
CACAATT407.0254545E-427.49862320