FastQCFastQC Report
Wed 25 May 2016
SRR1780194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111241
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAAAG150.002219608870.0000126
ATTAACG150.002219608870.0000118
CTTGCTG150.002219608870.0000162
GTGTCAT150.002219608870.0000168
CCATAGG150.002219608870.0000145
GTTTGCA200.006935618452.522
AGGTACT200.006935618452.515
TCCATAG200.006935618452.544
AACTTTT200.006935618452.522
ATTCGCA200.006935618452.541
TAACGAA200.006935618452.520
CTAACTC200.006935618452.533
AGCATTT200.006935618452.535
AATTCAA200.006935618452.540
TTGCATT200.006935618452.524
AAAGCTC200.006935618452.539
GTCATGC350.001250884440.070
AAGAGAC350.001250884440.05
CTTAGGC350.001250884440.053
AGAGACA350.001250884440.06
Produced by FastQC (version 0.11.5)