##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780194_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 111241 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.531890220332432 34.0 34.0 34.0 23.0 34.0 2 31.642002499078576 34.0 34.0 34.0 23.0 34.0 3 31.44849470968438 34.0 34.0 34.0 23.0 34.0 4 31.327046682428243 34.0 34.0 34.0 23.0 34.0 5 31.33912855871486 34.0 34.0 34.0 23.0 34.0 6 34.585647378214865 38.0 36.0 38.0 25.0 38.0 7 34.6342355786086 38.0 36.0 38.0 25.0 38.0 8 34.69346733668342 38.0 36.0 38.0 26.0 38.0 9 35.03952679317877 38.0 37.0 38.0 27.0 38.0 10-11 34.84997437994984 38.0 36.5 38.0 26.0 38.0 12-13 34.896602871243516 38.0 36.0 38.0 26.0 38.0 14-15 34.94001312465727 38.0 36.5 38.0 26.0 38.0 16-17 34.952508517542995 38.0 37.0 38.0 26.0 38.0 18-19 34.97233933531702 38.0 37.0 38.0 26.0 38.0 20-21 35.015385514333744 38.0 37.0 38.0 26.5 38.0 22-23 34.99905160866946 38.0 37.0 38.0 26.0 38.0 24-25 35.04123929126851 38.0 37.0 38.0 26.5 38.0 26-27 35.06530865418326 38.0 37.0 38.0 27.0 38.0 28-29 35.14208789924578 38.0 37.0 38.0 27.0 38.0 30-31 35.16343794104692 38.0 37.0 38.0 27.0 38.0 32-33 35.2107990758803 38.0 37.0 38.0 27.0 38.0 34-35 35.163010940210896 38.0 37.0 38.0 27.0 38.0 36-37 35.11437329761509 38.0 37.0 38.0 27.0 38.0 38-39 35.12323693602179 38.0 37.0 38.0 27.0 38.0 40-41 35.187457861759604 38.0 37.0 38.0 27.0 38.0 42-43 35.215900612184356 38.0 37.0 38.0 27.0 38.0 44-45 35.28214417346122 38.0 37.0 38.0 27.0 38.0 46-47 35.21486682068662 38.0 37.0 38.0 27.0 38.0 48-49 35.22119991729668 38.0 37.0 38.0 27.0 38.0 50-51 35.1560485792109 38.0 37.0 38.0 27.0 38.0 52-53 35.19677097473054 38.0 37.0 38.0 27.0 38.0 54-55 35.186846576352245 38.0 37.0 38.0 27.0 38.0 56-57 35.13745831123417 38.0 37.0 38.0 27.0 38.0 58-59 35.18024379500364 38.0 37.0 38.0 27.0 38.0 60-61 35.19280211432835 38.0 37.0 38.0 27.0 38.0 62-63 35.0709810231839 38.0 37.0 38.0 27.0 38.0 64-65 35.04547783640924 38.0 37.0 38.0 26.5 38.0 66-67 34.977661114157556 38.0 36.5 38.0 26.0 38.0 68-69 34.92595356028802 38.0 36.0 38.0 26.0 38.0 70-71 34.90056274215442 38.0 36.5 38.0 26.0 38.0 72-73 34.95820336027184 38.0 37.0 38.0 26.0 38.0 74-75 35.0093086182253 38.0 37.0 38.0 26.0 38.0 76 33.42641651908919 37.0 33.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.41589346391593907 1101 2 0.045471418712295986 1101 3 -0.0439448661059636 1101 4 -0.055268088483185096 1101 5 -0.32716743718194863 1101 6 -0.38367666186159965 1101 7 -0.1750479089775041 1101 8 -0.48126336370582123 1101 9 -0.37335071601752645 1101 10-11 -0.5642906604976048 1101 12-13 -0.19213850959223322 1101 14-15 -0.27925453858443205 1101 16-17 -0.43393092872349115 1101 18-19 -0.061864084269537045 1101 20-21 -0.10075949128829365 1101 22-23 -0.36932897947914256 1101 24-25 -0.8376299964102287 1101 26-27 -0.6133810447756218 1101 28-29 -0.8124114092207577 1101 30-31 -0.6205232104999681 1101 32-33 -0.2176532347750424 1101 34-35 -0.39313527588549135 1101 36-37 -0.9419294570757515 1101 38-39 -0.45465340985696656 1101 40-41 -0.26435967611370614 1101 42-43 -0.8052608890838755 1101 44-45 -0.4819956039449522 1101 46-47 -0.49452605322304777 1101 48-49 -0.5479187623111983 1101 50-51 -0.6540990599489973 1101 52-53 -0.12204494095958296 1101 54-55 -0.3602854746560453 1101 56-57 -0.5236486571102361 1101 58-59 -0.5711309857594173 1101 60-61 -0.6203387222247656 1101 62-63 -0.4035379641363619 1101 64-65 -0.58346118477305 1101 66-67 -0.6274873940184023 1101 68-69 -1.0997793190790688 1101 70-71 -1.0514999694614673 1101 72-73 -1.0157911950331169 1101 74-75 -0.8022197259616703 1101 76 -1.992122577730445 1102 1 0.27706428256293236 1102 2 -0.17988069396376005 1102 3 -0.05802937314821932 1102 4 0.023605150953436294 1102 5 -0.7947730709847676 1102 6 -0.31325412665033525 1102 7 -0.49617466954088485 1102 8 -0.844643645395962 1102 9 -0.6606746596794935 1102 10-11 -0.4586568576807011 1102 12-13 -0.555518791282374 1102 14-15 -0.7496770737957021 1102 16-17 -0.5888605061882828 1102 18-19 -0.3660894363822109 1102 20-21 -0.3570975194573123 1102 22-23 -0.6608782752537934 1102 24-25 -0.39678492598768855 1102 26-27 -0.5161979461840787 1102 28-29 -0.6997353528827261 1102 30-31 -0.3557344781056031 1102 32-33 -0.49793492491588864 1102 34-35 -0.3649662618009799 1102 36-37 -0.2855914289067343 1102 38-39 -0.39409002957528116 1102 40-41 -0.35450052118412856 1102 42-43 -0.5066693397881039 1102 44-45 -0.5355167307055169 1102 46-47 -0.7339626729413595 1102 48-49 -0.6408765087900719 1102 50-51 -0.8033948345968795 1102 52-53 -0.615002687438448 1102 54-55 -0.4433840662053399 1102 56-57 -0.8053387979553079 1102 58-59 -0.7528211266044877 1102 60-61 -0.5907612574360286 1102 62-63 -0.834523879629316 1102 64-65 -0.6426161143505169 1102 66-67 -0.6303042954268534 1102 68-69 -0.2913286148537182 1102 70-71 -0.5712182793206253 1102 72-73 -0.5425517584133956 1102 74-75 -0.6458816977926602 1102 76 -0.5047986340684716 1103 1 0.035310156699722484 1103 2 0.4465092244943598 1103 3 0.4134231647941178 1103 4 0.47613529023789525 1103 5 -0.07635396911593517 1103 6 -0.34699451914379864 1103 7 0.5072586728960076 1103 8 -0.010801813239588398 1103 9 -0.07574235114685735 1103 10-11 0.23860427142077612 1103 12-13 0.05424672703528444 1103 14-15 -0.35701584325455826 1103 16-17 0.08116797494784578 1103 18-19 0.12175731169628534 1103 20-21 0.36583564741093255 1103 22-23 -0.052521207640353396 1103 24-25 0.07428136093049886 1103 26-27 0.004585480135617104 1103 28-29 -0.07045440403172165 1103 30-31 -0.05112287496876888 1103 32-33 0.32434668719444204 1103 34-35 -0.14672272857949054 1103 36-37 0.12139620325744005 1103 38-39 -0.0260318188148716 1103 40-41 -0.14184007094809203 1103 42-43 -0.039309502091562365 1103 44-45 -0.03716707599051716 1103 46-47 0.2448442405618252 1103 48-49 0.27980937939220496 1103 50-51 -0.33877386779886365 1103 52-53 0.2457245569140838 1103 54-55 0.08733654555386039 1103 56-57 0.22409431039005767 1103 58-59 -0.09220312496584882 1103 60-61 0.32492287505976947 1103 62-63 0.0648986945972041 1103 64-65 0.16901433315445757 1103 66-67 0.2592396162426098 1103 68-69 0.35140869543459985 1103 70-71 0.19742906178975517 1103 72-73 0.26497428426930014 1103 74-75 0.16126589691687343 1103 76 -0.2299517689442112 1104 1 -0.30458673465146546 1104 2 -0.7115317111781572 1104 3 -0.7950794513954804 1104 4 -0.521387024320429 1104 5 -0.8198904418767867 1104 6 -0.6766344083404761 1104 7 -0.4840463440322793 1104 8 -0.4862711884319566 1104 9 -0.7839140962992133 1104 10-11 -0.3581678122972889 1104 12-13 -0.7582378836141359 1104 14-15 -0.43844859179257156 1104 16-17 -0.4463135578314734 1104 18-19 -0.43758787143698896 1104 20-21 -0.7870075351067669 1104 22-23 -0.5372280405120051 1104 24-25 -0.4209633297435573 1104 26-27 -0.6421173515048935 1104 28-29 -0.6838315970141835 1104 30-31 -0.514380800484318 1104 32-33 -0.3295468184996153 1104 34-35 -0.5089803463080216 1104 36-37 -0.6832244492510213 1104 38-39 -0.660365459935214 1104 40-41 -0.9302634319822545 1104 42-43 -0.6135746606018699 1104 44-45 -0.508423616464512 1104 46-47 -0.2785244882778244 1104 48-49 -0.8573866809340416 1104 50-51 -0.5861999989161291 1104 52-53 -0.49041348556052355 1104 54-55 -0.7075272586936023 1104 56-57 -0.2691690013981898 1104 58-59 -0.7000824724573818 1104 60-61 -0.5220992856050444 1104 62-63 -0.3845434414446629 1104 64-65 -0.3011841894678895 1104 66-67 -0.32469005442528953 1104 68-69 -0.6908004458396348 1104 70-71 -0.32704770029088337 1104 72-73 -0.42862374589383023 1104 74-75 -0.8561125272136252 1104 76 -0.1348455824252781 1105 1 -1.0137407750555028 1105 2 -0.56216302430947 1105 3 -0.9744986433146714 1105 4 -0.2254905596739647 1105 5 -0.15606468430102893 1105 6 -0.5885662265222962 1105 7 -0.5206625056484384 1105 8 -0.7878494712602375 1105 9 -0.5714140962992147 1105 10-11 -0.6663433173477955 1105 12-13 -1.4910661664424225 1105 14-15 -1.083840005933979 1105 16-17 -0.8195143154072326 1105 18-19 -0.7625563057804214 1105 20-21 -0.7029481916724194 1105 22-23 -0.6262431920271538 1105 24-25 -0.34217545095567914 1105 26-27 -0.36705422019176126 1105 28-29 -0.5998353848929696 1105 30-31 -0.49986059846411024 1105 32-33 -0.4515480811258783 1105 34-35 -0.6757417099443828 1105 36-37 -0.4680098027863764 1105 38-39 -0.4599551063998675 1105 40-41 -0.4884073713761907 1105 42-43 -0.7669206201978369 1105 44-45 -0.5339602326261286 1105 46-47 -0.47735655898488716 1105 48-49 -0.8210861758835364 1105 50-51 -0.37900302921915596 1105 52-53 -0.20906878859082667 1105 54-55 -0.6352734708148162 1105 56-57 -0.5008293549335363 1105 58-59 -0.5823425734674856 1105 60-61 -0.6533492856050458 1105 62-63 -0.5350800576062795 1105 64-65 -1.104972068255769 1105 66-67 -0.5445448524050818 1105 68-69 -0.3382436276578247 1105 70-71 -0.7608229528161345 1105 72-73 -0.464450766095851 1105 74-75 -0.7742627797388764 1105 76 -0.604542552122247 1106 1 -0.5634527924901249 1106 2 -0.2779320155921603 1106 3 0.20861156839142936 1106 4 -0.4761054413676149 1106 5 -0.7977443231553245 1106 6 -0.3670453672445859 1106 7 -1.7489626301814383 1106 8 -0.37333359330258276 1106 9 -0.05720176753209216 1106 10-11 -0.6138565489791787 1106 12-13 -0.3403345350601015 1106 14-15 -0.5795825339539036 1106 16-17 -0.2638363888816997 1106 18-19 -0.3824699110108085 1106 20-21 -0.3462350845740332 1106 22-23 -0.05943123685904084 1106 24-25 -0.4800957498348808 1106 26-27 -0.5399293758580086 1106 28-29 -0.04370602623792763 1106 30-31 -0.6549820181403305 1106 32-33 -0.2274159204783004 1106 34-35 -0.18329541480117229 1106 36-37 -0.07818259232561076 1106 38-39 -0.47687991960036413 1106 40-41 -0.1697040712516582 1106 42-43 -0.3627565479686865 1106 44-45 -0.20536045055887797 1106 46-47 -0.2848791305913636 1106 48-49 0.011486987254684777 1106 50-51 -0.35714748750060465 1106 52-53 -0.1062239878436273 1106 54-55 -0.4384062541273934 1106 56-57 -0.29289426776043825 1106 58-59 -0.22276131568416702 1106 60-61 -0.30052394313929653 1106 62-63 -0.2455213714294331 1106 64-65 -0.20335313923957443 1106 66-67 -0.6411474364648626 1106 68-69 0.004233800735704563 1106 70-71 -0.0786078220564903 1106 72-73 0.006840485460813284 1106 74-75 -0.30715895034909124 1106 76 0.12908135821398758 1107 1 -0.4782457384828831 1107 2 -0.4863401402969316 1107 3 -0.278508570169425 1107 4 -1.1629579055464845 1107 5 -0.37736170624460286 1107 6 -0.4553700405243859 1107 7 -0.1545444283234616 1107 8 -0.1751600773208466 1107 9 -0.03333938365553735 1107 10-11 -0.3587904176612753 1107 12-13 -0.18836240468693433 1107 14-15 -0.6762838408347136 1107 16-17 -0.13611240840619132 1107 18-19 0.14263243507642187 1107 20-21 -0.0014232439190209334 1107 22-23 0.25803058017765323 1107 24-25 0.14972632542885833 1107 26-27 -0.11199283043209363 1107 28-29 0.015450012181219108 1107 30-31 -0.15183290776400327 1107 32-33 0.09526161061916127 1107 34-35 0.25308861920921544 1107 36-37 0.1756357040056784 1107 38-39 -0.24925434882410258 1107 40-41 0.14459288985683116 1107 42-43 0.09988319380425992 1107 44-45 -0.25119181569822757 1107 46-47 0.12597742743099616 1107 48-49 -0.3037468341907541 1107 50-51 -0.031171263283944484 1107 52-53 -0.1468790028018958 1107 54-55 -0.116578982831534 1107 56-57 0.14227735875507364 1107 58-59 0.14091369612499705 1107 60-61 0.07330301324553545 1107 62-63 0.3338952558733439 1107 64-65 0.6387583392677456 1107 66-67 -0.024642161704974797 1107 68-69 -0.05243837687411457 1107 70-71 -0.01536187653609744 1107 72-73 0.12118468322493925 1107 74-75 0.16175145746070285 1107 76 -0.04289155943677514 1108 1 0.2029958479311169 1108 2 -0.04013531478176091 1108 3 -0.29740677872280585 1108 4 0.2778622249288176 1108 5 0.386566014579671 1108 6 0.635677903971839 1108 7 0.5422299322439983 1108 8 0.15517025300948717 1108 9 0.20542524304012488 1108 10-11 0.5164943498648711 1108 12-13 0.2348927470164881 1108 14-15 0.4362511079071325 1108 16-17 0.37992518090726435 1108 18-19 0.17971693336781414 1108 20-21 0.5695615339623075 1108 22-23 0.29028697450301166 1108 24-25 -0.08657894535917876 1108 26-27 0.3566439097563716 1108 28-29 0.19278251709992844 1108 30-31 0.20971829861478852 1108 32-33 0.3025853136325196 1108 34-35 0.08433797201029591 1108 36-37 -0.13408781261438918 1108 38-39 0.43768258811282834 1108 40-41 0.39477410555528536 1108 42-43 0.49644786192065027 1108 44-45 0.007079386538492827 1108 46-47 0.08401304925972397 1108 48-49 0.15387458032721213 1108 50-51 0.22862732841119993 1108 52-53 -0.3707062783533104 1108 54-55 0.20677454310819599 1108 56-57 0.049637304908124236 1108 58-59 0.08670431432940262 1108 60-61 0.11175957325381347 1108 62-63 0.131334936933726 1108 64-65 0.29333533005163304 1108 66-67 0.394341477106245 1108 68-69 0.06922207668288394 1108 70-71 0.4820363837932007 1108 72-73 -0.32351864078873405 1108 74-75 0.3424235088224066 1108 76 0.06034961276991169 1109 1 0.3513160431263138 1109 2 0.3162460665996214 1109 3 -0.04126000695103471 1109 4 0.49666853123512666 1109 5 0.671776224789884 1109 6 0.4046155916595211 1109 7 0.4583147670788321 1109 8 0.19879825601248768 1109 9 0.3442109037007839 1109 10-11 0.5593669099249254 1109 12-13 0.26433156083030696 1109 14-15 0.6853361304296541 1109 16-17 0.5510822755018552 1109 18-19 0.2594607396741253 1109 20-21 0.24719385378212166 1109 22-23 0.5153761261546563 1109 24-25 0.04362000358977269 1109 26-27 0.6332298707173294 1109 28-29 0.06252256965248648 1109 30-31 0.3342303106267934 1109 32-33 0.3431962370559418 1109 34-35 0.44883215369197416 1109 36-37 -0.03704389369547556 1109 38-39 0.37939148450922744 1109 40-41 0.7093199013510798 1109 42-43 0.5398975616203501 1109 44-45 0.4479999946466009 1109 46-47 0.4544616228332927 1109 48-49 0.3235160968437327 1109 50-51 0.5236958344172038 1109 52-53 0.4944823477728164 1109 54-55 0.49455607463973195 1109 56-57 0.6566990541573645 1109 58-59 0.7037369719870554 1109 60-61 0.6357132143949542 1109 62-63 0.6843801696664542 1109 64-65 0.44985747719878333 1109 66-67 0.5161085566858219 1109 68-69 0.07013705416036231 1109 70-71 0.3700009108202309 1109 72-73 0.08057764299505266 1109 74-75 0.5737485838974834 1109 76 0.15994608424138335 1110 1 0.04787257178873361 1110 2 0.10876199016649934 1110 3 0.2992057573801752 1110 4 -0.04640471717251771 1110 5 -0.011504029987190023 1110 6 -0.14478727458251228 1110 7 0.010822728862272868 1110 8 -0.023355406407894463 1110 9 0.5125827189874101 1110 10-11 0.29948434623066333 1110 12-13 0.3990250321678843 1110 14-15 0.14985643297742257 1110 16-17 0.23566627231714676 1110 18-19 -0.23849905331950794 1110 20-21 0.2476616085591985 1110 22-23 0.24130168347949876 1110 24-25 0.30982223288913957 1110 26-27 0.04849659046255539 1110 28-29 0.43482551551235815 1110 30-31 0.25516980107265397 1110 32-33 0.22600873705594182 1110 34-35 0.270796723755673 1110 36-37 0.34215594706886066 1110 38-39 0.4773910864200488 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0.13537793933669917 2116 12-13 0.5170323526855114 2116 14-15 0.25515372092738886 2116 16-17 0.32972339541136364 2116 18-19 0.664756271348331 2116 20-21 0.617888141112445 2116 22-23 0.688544960996289 2116 24-25 0.6044514515535724 2116 26-27 0.4488265901743489 2116 28-29 0.47836674386063294 2116 30-31 0.38688868166752144 2116 32-33 0.0789499135265288 2116 34-35 0.21172101115804054 2116 36-37 0.2576817850375619 2116 38-39 0.31824188043682256 2116 40-41 -0.1714210488751604 2116 42-43 0.25044620415428653 2116 44-45 0.19663545844750274 2116 46-47 0.1666363965889488 2116 48-49 0.1975404181106981 2116 50-51 0.41110392265249374 2116 52-53 -0.06132223367514911 2116 54-55 0.645545893644254 2116 56-57 0.2197010337953813 2116 58-59 0.5090678543400031 2116 60-61 0.06738035240400109 2116 62-63 0.4364658597117028 2116 64-65 0.24138745457434396 2116 66-67 0.2861382512559629 2116 68-69 0.26841901117393974 2116 70-71 0.23078286783380264 2116 72-73 0.3018941022710777 2116 74-75 0.43107324453096396 2116 76 0.31010162722780876 2117 1 0.3015726856383907 2117 2 0.5054972743290911 2117 3 0.24248093507794977 2117 4 0.06242940080034387 2117 5 0.10885351947587196 2117 6 0.3668438525290867 2117 7 0.5992869893010564 2117 8 0.6944051400704581 2117 9 0.7404820389664835 2117 10-11 0.6852500620988451 2117 12-13 0.3868708120380333 2117 14-15 0.6495948864683001 2117 16-17 0.6387709832313249 2117 18-19 0.7154918387079405 2117 20-21 0.05604802044879165 2117 22-23 0.8936294474106958 2117 24-25 0.7150270084206909 2117 26-27 0.5929671895579105 2117 28-29 0.7767360358360591 2117 30-31 0.8618269289842857 2117 32-33 0.36852081435062445 2117 34-35 0.7346186875084015 2117 36-37 0.43458956041080654 2117 38-39 0.3678954096300018 2117 40-41 0.3862220752641292 2117 42-43 0.5584664022000609 2117 44-45 0.5161536782214782 2117 46-47 0.66966391751928 2117 48-49 0.6824683915297243 2117 50-51 1.0172420300693759 2117 52-53 0.3786082535699151 2117 54-55 0.842170808939251 2117 56-57 0.5307706121283857 2117 58-59 0.8264875758517931 2117 60-61 0.7891325984529232 2117 62-63 0.7674493121785275 2117 64-65 0.28589310521810063 2117 66-67 1.0367229890529757 2117 68-69 0.5998396507787191 2117 70-71 0.98007790357385 2117 72-73 -0.18949331111122092 2117 74-75 0.560010661192166 2117 76 0.8796471711979095 2118 1 0.6146858884871413 2118 2 0.31547287072332963 2118 3 0.5104474672757675 2118 4 0.5996324487608966 2118 5 0.7823432350991588 2118 6 0.735736725680141 2118 7 0.655415282542755 2118 8 0.36435882302279765 2118 9 0.12897250163892693 2118 10-11 0.027585337759980177 2118 12-13 0.4146859422736 2118 14-15 0.2577427025946051 2118 16-17 0.4642104971513419 2118 18-19 0.4293222087462851 2118 20-21 0.497628776462534 2118 22-23 0.5218355333711528 2118 24-25 0.37010196235266335 2118 26-27 0.04698631401630138 2118 28-29 0.29172682222980484 2118 30-31 0.474307630214426 2118 32-33 0.2878644071590344 2118 34-35 0.08302996297032905 2118 36-37 0.5013259516653576 2118 38-39 0.7541820772927323 2118 40-41 0.43026062300056367 2118 42-43 0.3379001389399434 2118 44-45 0.5327132678424746 2118 46-47 0.24006084963741614 2118 48-49 0.3508034679777552 2118 50-51 0.5129838498811168 2118 52-53 0.6291311879789987 2118 54-55 0.40840916742323685 2118 56-57 -0.17534024996633946 2118 58-59 0.2263207348736529 2118 60-61 -0.23936732684215656 2118 62-63 0.18107797894479916 2118 64-65 0.216255673075068 2118 66-67 0.3038179639023184 2118 68-69 0.6497923376655166 2118 70-71 0.6147335139130021 2118 72-73 0.5015022563971172 2118 74-75 0.34533363544387896 2118 76 0.6452231460970594 2119 1 0.14819104312631382 2119 2 0.39238243023598685 2119 3 0.17891044759441854 2119 4 0.3284867130533087 2119 5 0.5910944066080646 2119 6 0.5960928643867973 2119 7 0.7179738579879285 2119 8 0.30021871055794236 2119 9 0.3376768127916918 2119 10-11 0.7981453190158376 2119 12-13 0.7759792881030378 2119 14-15 1.1860463577023808 2119 16-17 0.6847470482291342 2119 18-19 0.34397778512867205 2119 20-21 0.6535858992366741 2119 22-23 0.40784771706375267 2119 24-25 0.3595290944988676 2119 26-27 0.6159003252627855 2119 28-29 0.6601077969252103 2119 30-31 0.8410484924449833 2119 32-33 0.6635087370559418 2119 34-35 0.6438605627828835 2119 36-37 0.44960383357725675 2119 38-39 -0.09489828821804736 2119 40-41 0.8212517195329028 2119 42-43 0.5228521070748968 2119 44-45 0.30552840373750456 2119 46-47 0.543950259196933 2119 48-49 0.21584564229827663 2119 50-51 0.3340651525990168 2119 52-53 0.37189712050008694 2119 54-55 -0.011693925360269475 2119 56-57 0.4636592814300968 2119 58-59 0.44890742653251436 2119 60-61 0.26710525984950095 2119 62-63 0.5259994878482743 2119 64-65 0.3921870226533244 2119 66-67 0.10460287486763775 2119 68-69 0.06090409961490906 2119 70-71 0.27326795627477196 2119 72-73 0.488674233904149 2119 74-75 0.21011222026112364 2119 76 0.4125029024232063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 111.0 14 477.0 15 1029.0 16 1280.0 17 1415.0 18 1291.0 19 1125.0 20 862.0 21 605.0 22 492.0 23 432.0 24 433.0 25 463.0 26 554.0 27 665.0 28 758.0 29 1003.0 30 1357.0 31 1639.0 32 2333.0 33 3215.0 34 4736.0 35 7740.0 36 16380.0 37 60842.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.817153747269444 21.670966639997843 11.669258636653753 26.842620976078962 2 20.517614908172348 21.63410972573062 31.344558211450813 26.503717154646218 3 20.26411125394414 21.31498278512419 27.51503492417364 30.905871036758032 4 16.189174854594977 17.559173326381462 30.369198407062143 35.88245341196142 5 17.006319612373137 31.52165118975917 31.700542066324466 19.771487131543225 6 31.403888853929757 32.372057065290676 16.929908936453288 19.294145144326283 7 29.81095099828301 25.48161199557717 19.757103945487724 24.950333060652095 8 26.02637516742928 30.20648861480929 19.82182828273748 23.94530793502396 9 24.80200645445474 16.064220925737814 20.174216341097257 38.95955627871019 10-11 28.063393892539622 22.30382682643989 23.628428367238698 26.00435091378179 12-13 26.72485864024955 20.036227649877294 23.405938457942664 29.832975251930492 14-15 25.2847421364425 21.207108889707932 21.325320700101578 32.18282827374799 16-17 26.09963952139948 23.367283645418503 22.58789475103604 27.94518208214597 18-19 25.683875549482654 22.779370915399895 23.094003110363985 28.442750424753466 20-21 25.390818133601815 23.093104161235516 23.450885914366108 28.065191790796558 22-23 26.873634721011136 22.39462068841524 22.935788063753474 27.79595652682015 24-25 26.307296770075784 23.149288481764817 22.324502656394674 28.218912091764725 26-27 25.23934520545482 23.46437015129314 23.416276372920056 27.88000827033198 28-29 26.087503708165155 22.978038672791506 24.030708102228495 26.903749516814845 30-31 26.007047761167197 22.38608067169479 23.749786499581987 27.857085067556024 32-33 25.256874713459965 23.49403547253261 23.717873805521343 27.53121600848608 34-35 25.872654866461108 23.0993968051348 23.88732571623772 27.140622612166375 36-37 26.018734099837292 22.387878569951727 23.547522945676505 28.04586438453448 38-39 24.98943734774049 22.665653850648592 24.93370250177542 27.411206299835495 40-41 25.029440583957353 22.299781555361783 25.34317382979297 27.32760403088789 42-43 24.514342733344723 22.234607743547794 24.93145512895425 28.319594394153235 44-45 23.731807517012612 22.297084707976374 25.893780170980126 28.077327604030888 46-47 24.181282081247023 22.475526109977437 26.170207027984286 27.172984780791253 48-49 23.605055689898506 22.592838971242617 26.00210354096062 27.800001797898254 50-51 23.278737156264327 23.451335388930342 25.421382403969762 27.848545050835572 52-53 22.987927113204666 23.476505964527465 25.490152012297624 28.045414909970244 54-55 22.79600147427657 23.269298190415405 25.56836058647441 28.366339748833614 56-57 22.93039436898266 23.25851080087378 25.48655621578375 28.324538614359813 58-59 23.324583561816237 23.16816641346266 24.957974128244082 28.549275896477017 60-61 23.4063879325069 22.589243174728743 25.041576397191683 28.962792495572675 62-63 22.88105501143918 22.880605534854663 25.436778871004716 28.80156058270145 64-65 23.815409785960213 22.944328080473923 24.295898095126798 28.944364038439062 66-67 23.42706376246168 23.191539090802852 23.597414622306523 29.783982524428943 68-69 23.371328916496616 23.682365314946825 23.781699193642634 29.164606574913925 70-71 24.49501532708264 23.245476038510983 23.830691921144183 28.428816713262194 72-73 24.07205976213806 23.001411350131697 23.359193103262285 29.567335784467957 74-75 23.44414379590259 23.967781663235677 23.56729982650282 29.020774714358915 76 23.49223757427567 23.21266439532187 23.1973822601379 30.09771577026456 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 2.0 16 4.0 17 5.0 18 5.0 19 9.0 20 18.5 21 24.0 22 36.0 23 55.0 24 88.5 25 115.0 26 186.0 27 277.5 28 359.5 29 421.0 30 512.0 31 699.0 32 1018.5 33 1242.0 34 1424.0 35 1813.5 36 2232.0 37 2443.0 38 2747.0 39 3470.0 40 4270.5 41 4718.0 42 4784.0 43 4939.0 44 5340.5 45 5536.0 46 5485.0 47 5449.0 48 5209.0 49 4618.5 50 4232.0 51 4040.0 52 3433.5 53 2778.0 54 2537.0 55 2513.5 56 2259.5 57 2003.5 58 1978.0 59 1980.5 60 2044.0 61 2243.5 62 2382.0 63 2503.0 64 2696.5 65 2987.5 66 3231.0 67 3256.0 68 3357.0 69 3496.0 70 3325.5 71 3117.0 72 2972.0 73 2545.5 74 2055.0 75 1846.0 76 1563.5 77 1044.5 78 606.5 79 405.0 80 297.5 81 120.0 82 34.5 83 19.0 84 16.0 85 7.5 86 3.5 87 5.0 88 3.0 89 3.0 90 3.5 91 3.5 92 5.0 93 4.0 94 2.5 95 1.5 96 1.0 97 1.0 98 2.5 99 11.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.4947456423441E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 111241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.08105824291403 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3010887533489 48.131534236477556 2 13.030838511087042 16.43998166143778 3 4.279484694750043 8.0986326983756 4 2.012198597731289 5.077264677591895 5 1.2155845636436184 3.8340180329195173 6 0.8493416177392693 3.2146420834045 7 0.5572023029128428 2.46042376461916 8 0.423245739041213 2.135903129241916 9 0.3106652225959072 1.7637381900558247 >10 1.0189249273214387 8.798015120324342 >50 0.0014250698284215928 0.045846405551909816 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.00269684738540646 0.0 3 0.0 0.0 0.0 0.00269684738540646 0.0 4 0.0 0.0 0.0 0.026069524725595778 0.0 5 0.0 0.0 0.0 0.026069524725595778 0.0 6 0.0 0.0 0.0 0.026069524725595778 0.0 7 0.0 0.0 0.0 0.026069524725595778 0.0 8 0.0 0.0 0.0 0.026069524725595778 0.0 9 0.0 0.0 0.0 0.0269684738540646 0.0 10 0.0 0.0 0.0 0.02966532123947106 0.0 11 0.0 0.0 0.0 0.02966532123947106 0.0 12 0.0 0.0 0.0 0.030564270367939878 0.0 13 0.0 0.0 0.0 0.030564270367939878 0.0 14 0.0 0.0 0.0 0.0314632194964087 0.0 15 0.0 0.0 0.0 0.0314632194964087 0.0 16 0.0 0.0 0.0 0.0314632194964087 0.0 17 0.0 0.0 0.0 0.03505901601028398 0.0 18 0.0 0.0 0.0 0.03505901601028398 0.0 19 0.0 0.0 0.0 0.042250609038034535 0.0 20 0.0 0.0 0.0 0.044947456423440994 0.0 21 0.0 0.0 0.0 0.045846405551909816 0.0 22 0.0 0.0 0.0 0.04764430380884746 0.0 23 0.0 0.0 0.0 0.0494422020657851 0.0 24 0.0 0.0 0.0 0.05663379509353566 0.0 25 0.0 0.0 0.0 0.057532744222004474 0.0 26 0.0 0.0 0.0 0.06562328637822386 0.0 27 0.0 0.0 0.0 0.1078738954162584 0.0 28 0.0 0.0 0.0 0.15641714835357468 0.0 29 0.0 0.0 0.0 0.21754568908945443 0.0 30 0.0 0.0 0.0 0.2759773824399277 0.0 31 0.0 0.0 0.0 0.3514891092313086 0.0 32 0.0 0.0 0.0 0.44767666597747235 0.0 33 0.0 0.0 0.0 0.5717316457061695 0.0 34 0.0 0.0 0.0 0.7146645571327118 0.0 35 0.0 0.0 0.0 0.9304123479652286 0.0 36 0.0 0.0 0.0 1.2801035589395997 0.0 37 0.0 0.0 0.0 1.7547486987711365 0.0 38 0.0 0.0 0.0 2.260857058099082 0.0 39 0.0 0.0 0.0 2.7984286369234366 0.0 40 0.0 0.0 0.0 3.3665644861157307 0.0 41 0.0 0.0 0.0 3.932902437051087 0.0 42 0.0 0.0 0.0 4.552278386566104 0.0 43 0.0 0.0 0.0 5.190532267778966 0.0 44 0.0 0.0 0.0 5.800019776880826 0.0 45 0.0 0.0 0.0 6.477827419746316 0.0 46 0.0 0.0 0.0 7.164624553896495 0.0 47 0.0 0.0 0.0 7.766021520842136 0.0 48 0.0 0.0 0.0 8.353934250860744 0.0 49 0.0 0.0 0.0 8.925665896566914 0.0 50 0.0 0.0 0.0 9.504589135300833 0.0 51 0.0 0.0 0.0 10.048453358024469 0.0 52 0.0 0.0 0.0 10.632770291529202 0.0 53 0.0 0.0 0.0 11.253944139301156 0.0 54 0.0 0.0 0.0 11.822079988493451 0.0 55 0.0 0.0 0.0 12.39471058332809 0.0 56 0.0 0.0 0.0 12.952059042978759 0.0 57 0.0 0.0 0.0 13.494125367445456 0.0 58 0.0 0.0 0.0 14.025404302370529 0.0 59 0.0 0.0 0.0 14.623205472802294 0.0 60 0.0 0.0 0.0 15.132010679515647 0.0 61 0.0 0.0 0.0 15.69205598655172 0.0 62 0.0 0.0 0.0 16.290756106111957 0.0 63 0.0 0.0 0.0 16.87956778525903 0.0 64 0.0 0.0 0.0 17.415341465826447 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAAAG 15 0.0022196088 70.00001 26 ATTAACG 15 0.0022196088 70.00001 18 CTTGCTG 15 0.0022196088 70.00001 62 GTGTCAT 15 0.0022196088 70.00001 68 CCATAGG 15 0.0022196088 70.00001 45 GTTTGCA 20 0.0069356184 52.5 22 AGGTACT 20 0.0069356184 52.5 15 TCCATAG 20 0.0069356184 52.5 44 AACTTTT 20 0.0069356184 52.5 22 ATTCGCA 20 0.0069356184 52.5 41 TAACGAA 20 0.0069356184 52.5 20 CTAACTC 20 0.0069356184 52.5 33 AGCATTT 20 0.0069356184 52.5 35 AATTCAA 20 0.0069356184 52.5 40 TTGCATT 20 0.0069356184 52.5 24 AAAGCTC 20 0.0069356184 52.5 39 GTCATGC 35 0.0012508844 40.0 70 AAGAGAC 35 0.0012508844 40.0 5 CTTAGGC 35 0.0012508844 40.0 53 AGAGACA 35 0.0012508844 40.0 6 >>END_MODULE