FastQCFastQC Report
Wed 25 May 2016
SRR1780194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111241
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT30962.7831465017394663No Hit
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG15091.3565142348594492Illumina PCR Primer Index 10 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG8740.7856815382817486No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC7200.6472433724975504No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2570.2310299260164867No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT1750.1573160974820435Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA452.6739144E-854.4444432
CTGAATT200.006935618452.524
GTTATAT200.006935618452.540
CCACATA200.006935618452.569
GCATACT200.006935618452.552
TTTATAC801.4006218E-939.3753
CACATAG400.0024100835.070
TATTCTG552.9762226E-431.8181820
TATGCCG7700.031.3636443
CGTATGC7750.031.16129141
TTAGAGG450.004291907431.1111094
TTGCCGC450.004291907431.11110938
TCGTATG7850.030.7643340
CTCGTAT7700.030.45454839
GTATGCC7950.030.37735742
TCTCGTA7400.030.27027138
ATGCCGT8000.030.18749844
TATCTCG7450.030.06711436
CCGTCTT7600.029.93421247
ATCTCGT7500.029.86666737