FastQCFastQC Report
Wed 25 May 2016
SRR1780193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739907
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAGGC609.663112E-625.6950314
GACTCTT500.002556966622.03326221
ACGATTC500.002563008322.0243114
CGGTATG500.00259135821.98262843
ATCTAGG602.890658E-421.9781721
TATACAA956.924638E-720.8214262
CGCAAAG755.442462E-520.54628412
GTAGTCA755.499451E-520.51850942
CTATACA755.5109103E-520.512961
GTCAAAC654.9424614E-420.29989229
ATGCCAT550.004470917420.0085540
CCATTGC550.004502529719.98420743
ACATAAG550.004504291319.9828599
GTATAGA1252.3104803E-819.3407921
CGATTCT808.88817E-519.27649315
AATCGTC708.140254E-418.84989728
TAGAGCC708.173622E-418.8384324
AGCCTTA600.00735089418.35856616
TTCGGTA600.007405636418.33496923
GTATCCA600.007446185718.317629
Produced by FastQC (version 0.11.5)