FastQCFastQC Report
Wed 25 May 2016
SRR1780193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739907
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT124301.6799408574320824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC39050.527769030432203No Hit
CACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCTG34610.4677614889438808RNA PCR Primer, Index 34 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG32430.4382983266815964No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC7780.10514834972503301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCATA250.00233502635.252631
AATTACT401.8198876E-532.99797428
CTTTATA1300.032.151872
TCTTCTG5050.030.93088344
CCGTCTT5150.030.33028441
TCTTTAT1350.029.3771921
CCCTTTG300.005701803629.377191
TTACATT300.00574341929.33351526
ACAGTCG300.00574531629.3315324
TACTGTG300.00574531629.33153231
AGCCTTA300.00574531629.3315326
CTATAAC454.069142E-529.331534
TATACCG454.069142E-529.331536
ATGCCGT21050.028.7412744
GTCTTCT5450.028.66072743
TTAATAC407.03206E-427.4983123
CGTCTTC5750.027.16538442
TATGCCG22800.026.5352543
TTAACGG508.336788E-526.40016235
TTTAACG508.3406216E-526.39837834
Produced by FastQC (version 0.11.5)