FastQCFastQC Report
Wed 25 May 2016
SRR1780192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68412
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT200.00692425752.51
AGTCAAT200.00692425752.533
AGGAGCG200.00692425752.511
TTATCGC200.00692425752.517
TCAGGAT200.00692425752.519
GCTCTTC200.00692425752.51
TTGAGAT200.00692425752.517
GATGGGA200.00692425752.524
ATGAAAG200.00692425752.569
ATTGAGA200.00692425752.516
GTAAAAC200.00692425752.566
TCACGAA305.8404944E-446.66666836
TCTTCCG404.549419E-543.7517
CTTCCGA503.4329532E-642.018
TCCCTGG400.002403614635.02
TAGATCT703.4435783E-530.039
CATCCTA604.936028E-429.1666686
TTCCGAC500.007163797527.99999819
ACCTCTG500.007163797527.9999988
TCTCGGT500.007163797527.99999843
Produced by FastQC (version 0.11.5)