##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780192_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68412 Sequences flagged as poor quality 0 Sequence length 76 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.056086651464657 34.0 31.0 34.0 12.0 34.0 2 30.062576740922644 34.0 31.0 34.0 12.0 34.0 3 29.99144886861954 34.0 31.0 34.0 11.0 34.0 4 29.879831023796996 34.0 31.0 34.0 11.0 34.0 5 29.889814652400165 34.0 31.0 34.0 11.0 34.0 6 32.56218207332047 37.0 31.0 38.0 11.0 38.0 7 32.963968309653275 38.0 34.0 38.0 12.0 38.0 8 32.8496024089341 37.0 33.0 38.0 11.0 38.0 9 33.21027012804771 38.0 34.0 38.0 12.0 38.0 10-11 32.888922995965615 38.0 33.5 38.0 11.0 38.0 12-13 32.97292141729521 38.0 34.0 38.0 11.0 38.0 14-15 32.90572560369526 38.0 34.0 38.0 11.0 38.0 16-17 32.912800385897214 38.0 34.0 38.0 11.0 38.0 18-19 32.94598169911711 38.0 34.0 38.0 11.0 38.0 20-21 32.98977518563994 38.0 34.0 38.0 11.0 38.0 22-23 32.92437730222768 38.0 34.0 38.0 11.0 38.0 24-25 32.95547564754722 38.0 34.0 38.0 11.0 38.0 26-27 32.92372683155002 38.0 34.0 38.0 11.0 38.0 28-29 32.98604046073788 38.0 34.0 38.0 11.0 38.0 30-31 32.990147927264225 38.0 34.0 38.0 11.0 38.0 32-33 33.053236274337834 38.0 34.0 38.0 11.0 38.0 34-35 33.03304244869321 38.0 34.0 38.0 11.0 38.0 36-37 32.96707448985558 38.0 34.0 38.0 11.0 38.0 38-39 32.9493729170321 38.0 34.0 38.0 11.0 38.0 40-41 33.02096854353037 38.0 34.0 38.0 11.0 38.0 42-43 33.071091329006606 38.0 34.0 38.0 11.0 38.0 44-45 33.1391714903818 38.0 34.0 38.0 11.0 38.0 46-47 33.014997368882646 38.0 34.0 38.0 11.0 38.0 48-49 32.9715400806876 38.0 34.0 38.0 11.0 38.0 50-51 32.90269251008595 38.0 34.0 38.0 11.0 38.0 52-53 32.964633397649536 38.0 34.0 38.0 11.0 38.0 54-55 32.93881921300357 38.0 34.0 38.0 11.0 38.0 56-57 32.86878033093609 38.0 33.5 38.0 11.0 38.0 58-59 32.90882447523826 38.0 34.0 38.0 11.0 38.0 60-61 32.94354791557037 38.0 33.5 38.0 11.0 38.0 62-63 32.804040226860785 38.0 33.0 38.0 11.0 38.0 64-65 32.72243904578144 38.0 33.0 38.0 11.0 38.0 66-67 32.69701222007835 38.0 32.0 38.0 11.0 38.0 68-69 32.65232707712097 38.0 32.0 38.0 11.0 38.0 70-71 32.55449336373735 38.0 32.0 38.0 11.0 38.0 72-73 32.59483716307081 38.0 32.0 38.0 11.0 38.0 74-75 32.66560691106824 38.0 32.0 38.0 11.0 38.0 76 30.90061685084488 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5061949393951259 1101 2 -0.3959030554904963 1101 3 0.08164531693043742 1101 4 -0.739225510842779 1101 5 -1.6470432480375017 1101 6 -1.378483354614243 1101 7 -1.099163366189078 1101 8 -1.5409000967372286 1101 9 -0.7831381204823842 1101 10-11 -1.0090261961336466 1101 12-13 -1.1337599108743603 1101 14-15 -1.5030877384792944 1101 16-17 -0.9875424700619035 1101 18-19 -0.9713238029460456 1101 20-21 -0.7483810552116594 1101 22-23 -0.9422169012319372 1101 24-25 -1.370597470229967 1101 26-27 -1.329696049002294 1101 28-29 -1.1674103776523133 1101 30-31 -1.212653465024026 1101 32-33 -1.2255737401265847 1101 34-35 -0.8669691920250173 1101 36-37 -1.4473903235210237 1101 38-39 -1.418362712652936 1101 40-41 -1.0548828791364926 1101 42-43 -1.2526574296328938 1101 44-45 -1.0301718191011346 1101 46-47 -0.7459126880105558 1101 48-49 -0.8390511192346395 1101 50-51 -1.1255772474977164 1101 52-53 -0.7756978137317532 1101 54-55 -0.3328658221879266 1101 56-57 -0.2907630986402481 1101 58-59 -0.8287057560527487 1101 60-61 -0.6727886543455597 1101 62-63 -1.328288000068408 1101 64-65 -0.5694922905414899 1101 66-67 -1.4196173739966902 1101 68-69 -1.4864840298293842 1101 70-71 -0.6278523874126591 1101 72-73 -0.5955374258041815 1101 74-75 -1.0004482495132265 1101 76 -2.6227039441848774 1102 1 0.281233169878778 1102 2 0.038227066723738545 1102 3 0.33501519471620256 1102 4 0.6142522030321302 1102 5 0.44115768654193843 1102 6 -0.13180830069619986 1102 7 -0.3334372698555015 1102 8 -0.29763985230876244 1102 9 0.025138299359454663 1102 10-11 0.26047416331998363 1102 12-13 0.5140878605131363 1102 14-15 0.1417720746048161 1102 16-17 0.8606108369114978 1102 18-19 0.33607644867149133 1102 20-21 0.473693890870301 1102 22-23 -0.07337254465393528 1102 24-25 0.2973347727606921 1102 26-27 -0.0577244458390993 1102 28-29 0.30358620753819565 1102 30-31 0.37658089874305034 1102 32-33 0.057495993877729745 1102 34-35 -0.05080725816448961 1102 36-37 0.10003508985064968 1102 38-39 0.09882366405446419 1102 40-41 -0.12037083743987864 1102 42-43 0.1927730160896033 1102 44-45 -0.008716930531754485 1102 46-47 -0.10714112079272553 1102 48-49 -0.014015173871584352 1102 50-51 -0.5492832144279802 1102 52-53 0.0206492387484829 1102 54-55 -0.20173264461783447 1102 56-57 0.06035570078461916 1102 58-59 -0.13132078121449808 1102 60-61 -0.2293352035907006 1102 62-63 -0.036703708192064255 1102 64-65 -0.22154117623523462 1102 66-67 0.38524423635557525 1102 68-69 0.4675283713208671 1102 70-71 0.021815117979144816 1102 72-73 -0.20541790747204658 1102 74-75 -0.7361599677015818 1102 76 -0.5206640448318929 1103 1 0.23926323146627126 1103 2 0.5665659271397772 1103 3 -0.08502873482425954 1103 4 0.47720926405973785 1103 5 0.8428761068012065 1103 6 -0.898378781767736 1103 7 0.19253284082971334 1103 8 -0.1971833296333685 1103 9 -0.029881296661045553 1103 10-11 -0.07004179343956451 1103 12-13 0.367203843113586 1103 14-15 0.5913823076036522 1103 16-17 0.5620299333411438 1103 18-19 0.35436965681999766 1103 20-21 -0.12839922252820912 1103 22-23 0.3603132299272289 1103 24-25 0.3236709629497554 1103 26-27 0.6290730045248267 1103 28-29 0.5546629119612945 1103 30-31 0.3801770915162095 1103 32-33 0.4143589078705787 1103 34-35 0.3173072170497804 1103 36-37 0.5582481911900672 1103 38-39 0.04369639759165267 1103 40-41 -0.013507923447022563 1103 42-43 0.4442696352377169 1103 44-45 0.1386623709024164 1103 46-47 0.3983070035881724 1103 48-49 0.008121869067380771 1103 50-51 0.15087613782663567 1103 52-53 -0.04354387895684653 1103 54-55 0.4371514589180663 1103 56-57 0.7850854479744171 1103 58-59 0.37226464451442354 1103 60-61 0.2308913350199191 1103 62-63 -0.05940063743104673 1103 64-65 0.7136519845382736 1103 66-67 0.06821938601748911 1103 68-69 0.14018869260947042 1103 70-71 0.2571396366922727 1103 72-73 0.19929375108344516 1103 74-75 0.3052500844109147 1103 76 -0.057013586155811424 1104 1 -0.06509465839758377 1104 2 -0.3571341759681914 1104 3 -0.7513587224092184 1104 4 -0.1295706109481536 1104 5 -1.1039496407315639 1104 6 -0.7402237831354945 1104 7 -0.2791335102003174 1104 8 -1.218499323993992 1104 9 -0.5801832557124555 1104 10-11 0.006121327575520752 1104 12-13 -0.432440539606354 1104 14-15 -0.016786368616280356 1104 16-17 -0.49929826563619173 1104 18-19 -0.10555501123547373 1104 20-21 -0.5457445079689478 1104 22-23 -0.9180467220960011 1104 24-25 0.17492193616272544 1104 26-27 0.04071578801913489 1104 28-29 0.1451664576128806 1104 30-31 -0.05735841760569116 1104 32-33 -0.2778217204567568 1104 34-35 -0.18870040031444546 1104 36-37 0.17790604107328534 1104 38-39 -0.14898924935824454 1104 40-41 -0.03445567506202707 1104 42-43 0.0418960477116741 1104 44-45 -0.24325796240987785 1104 46-47 -0.22904185906780583 1104 48-49 -0.3123124469480203 1104 50-51 -0.12131837851281801 1104 52-53 -0.4833835355441849 1104 54-55 -0.38637609616053226 1104 56-57 0.22904942331268074 1104 58-59 -0.2167238452694633 1104 60-61 0.14942551846792895 1104 62-63 -0.027763324971814995 1104 64-65 0.019987864007163125 1104 66-67 -0.20832039472025343 1104 68-69 0.031967410283016306 1104 70-71 0.1897406930228911 1104 72-73 0.5093646641150329 1104 74-75 0.27058266021631994 1104 76 0.40028077657381544 1105 1 -0.04053234369698444 1105 2 0.5943904188077198 1105 3 0.6532594255706101 1105 4 0.37604517818383343 1105 5 0.8953145718677114 1105 6 0.75608645945794 1105 7 0.11141811394216461 1105 8 -0.15924679742990122 1105 9 0.5603954194374126 1105 10-11 0.7686351235892843 1105 12-13 -0.3111546429195684 1105 14-15 0.4400402236060188 1105 16-17 0.17005186096252345 1105 18-19 0.2509123436088174 1105 20-21 0.18058586057397719 1105 22-23 0.4024103317246812 1105 24-25 0.6991596387747734 1105 26-27 0.49424215734112664 1105 28-29 0.4387348100983246 1105 30-31 0.4179061412464833 1105 32-33 0.3966464567381607 1105 34-35 0.08245889765780845 1105 36-37 -0.14608928451264092 1105 38-39 0.09153748785745108 1105 40-41 0.14887441215041974 1105 42-43 4.7554521216852663E-4 1105 44-45 0.5766797120691152 1105 46-47 0.7498150735643634 1105 48-49 0.6599007582717462 1105 50-51 -0.29304899376092663 1105 52-53 0.06771040719424093 1105 54-55 0.299983703878425 1105 56-57 -0.03095403921626172 1105 58-59 0.4953235913770797 1105 60-61 0.3020905837149641 1105 62-63 -0.17923814589414633 1105 64-65 -0.6169767965567985 1105 66-67 0.26341671276962586 1105 68-69 -0.2361080728479834 1105 70-71 0.2774292385443289 1105 72-73 -0.029220892811103738 1105 74-75 0.5127216374718273 1105 76 0.04515515169554618 1106 1 -0.2745285794392949 1106 2 -0.32739919093399905 1106 3 -0.6701719447552463 1106 4 -0.367100924902406 1106 5 -0.67186382220018 1106 6 -0.35552604876220073 1106 7 -2.007564175905678 1106 8 -0.6150167695381157 1106 9 -0.10134294197668225 1106 10-11 -0.48257238678510106 1106 12-13 -1.0754350120889384 1106 14-15 -1.330838934651542 1106 16-17 -0.6641734049165251 1106 18-19 -1.060013074201109 1106 20-21 -0.9604830979058079 1106 22-23 -1.0279962534586566 1106 24-25 -0.775352254918964 1106 26-27 -0.9886259349058619 1106 28-29 -1.3796964468462782 1106 30-31 -0.5203917793412884 1106 32-33 -0.9715251571306354 1106 34-35 -1.1933976977298357 1106 36-37 -0.6059636580812011 1106 38-39 -0.6271522783504011 1106 40-41 -0.5620714142855547 1106 42-43 -0.5749683424043326 1106 44-45 -0.9567337440183223 1106 46-47 -1.134910203652808 1106 48-49 -0.689483581509208 1106 50-51 -1.0766722051716187 1106 52-53 -0.38421594034198137 1106 54-55 -0.6422258700348138 1106 56-57 -0.6953568601419065 1106 58-59 -0.9509223552430299 1106 60-61 -0.8606682090014246 1106 62-63 -0.6369809518534666 1106 64-65 -1.170798878690178 1106 66-67 -1.5765229684022835 1106 68-69 -0.31530488003181745 1106 70-71 -0.42208775730592407 1106 72-73 -0.8730608891829092 1106 74-75 -1.1355697070031034 1106 76 -0.6531373265919456 1107 1 0.12159810089791279 1107 2 0.44045225586481607 1107 3 0.07088524367036442 1107 4 -0.4985203826376541 1107 5 0.09402818053392537 1107 6 -0.08993024106113268 1107 7 0.29268645066073873 1107 8 -0.026522807359938838 1107 9 0.3263399014956363 1107 10-11 -0.7230371851446407 1107 12-13 0.4751915543271039 1107 14-15 0.37786464247308515 1107 16-17 0.33468738341795046 1107 18-19 0.5735708856986435 1107 20-21 0.8461015455209449 1107 22-23 1.0965651500501181 1107 24-25 0.5999382440557497 1107 26-27 0.27300541619917595 1107 28-29 0.8505154465235094 1107 30-31 0.4962293188587594 1107 32-33 0.706294391741551 1107 34-35 1.0547112109053884 1107 36-37 0.9369732295925317 1107 38-39 0.294618056577832 1107 40-41 1.1260466080429978 1107 42-43 0.8176951344696732 1107 44-45 0.9183858732065602 1107 46-47 0.6845589237110588 1107 48-49 0.8181833129997926 1107 50-51 0.6789099319894589 1107 52-53 1.198775629491692 1107 54-55 0.9680270349549289 1107 56-57 0.5396169379897842 1107 58-59 1.0732631084161213 1107 60-61 0.6841939463870474 1107 62-63 0.8929803149499094 1107 64-65 0.6669545959053949 1107 66-67 0.6299705380912215 1107 68-69 0.5447201826248289 1107 70-71 0.4460029239426575 1107 72-73 0.7163444423276886 1107 74-75 0.5710718970069237 1107 76 0.31195722797629344 1108 1 -1.0129257086258967 1108 2 -1.1635953631828038 1108 3 -0.5381623753772544 1108 4 -0.23018704930431966 1108 5 -0.07954324803750268 1108 6 -0.5354064315373179 1108 7 -1.0599325969583084 1108 8 -1.3512847121218456 1108 9 -0.0929458127900773 1108 10-11 -0.6376800422874922 1108 12-13 -1.3488560647205148 1108 14-15 -1.273087738479294 1108 16-17 -1.3509078546772884 1108 18-19 -0.9248814952537359 1108 20-21 -1.152707978288582 1108 22-23 -1.2501015166165494 1108 24-25 -1.079347470229969 1108 26-27 -1.2435422028484453 1108 28-29 -0.802698839190775 1108 30-31 -0.7249611573317196 1108 32-33 -1.504419893972738 1108 34-35 -0.7505268843327073 1108 36-37 -1.2292172465979476 1108 38-39 -1.6703819434221714 1108 40-41 -1.3322867252903379 1108 42-43 -1.1756381988636662 1108 44-45 -0.9566141267934469 1108 46-47 -1.308893457241325 1108 48-49 -1.336935734619253 1108 50-51 -1.1269234013438698 1108 52-53 -0.9009862752702134 1108 54-55 -1.1991158221879274 1108 56-57 -0.9433592524864096 1108 58-59 -1.4594749868219736 1108 60-61 -1.3371155774224803 1108 62-63 -1.1601149231453292 1108 64-65 -1.47256921361841 1108 66-67 -1.0753866047659209 1108 68-69 -1.1750417221370775 1108 70-71 -1.260544695104965 1108 72-73 -1.3829412719580283 1108 74-75 -1.3283328648978383 1108 76 -1.5282808672618025 1109 1 0.6027959408586412 1109 2 0.15851803887905191 1109 3 -0.1357912413566389 1109 4 0.4214108888400112 1109 5 -0.11804840267667771 1109 6 1.0072224344420633 1109 7 0.7718715267530314 1109 8 0.484179205403926 1109 9 1.0780335686532183 1109 10-11 0.6229127412176609 1109 12-13 0.49686558476402354 1109 14-15 0.8654689625516312 1109 16-17 0.9342210113020926 1109 18-19 1.270969020210181 1109 20-21 0.8759002691340996 1109 22-23 0.9881459060638633 1109 24-25 0.4093638699762181 1109 26-27 0.8611742919969103 1109 28-29 0.534388789675198 1109 30-31 0.49838935813219365 1109 32-33 0.28037392046024934 1109 34-35 0.5048339404095614 1109 36-37 0.4829477018556574 1109 38-39 0.4781232112170031 1109 40-41 0.795806058214815 1109 42-43 0.8405473681466447 1109 44-45 0.4137466979488238 1109 46-47 0.9304106664700242 1109 48-49 0.7736570488859087 1109 50-51 1.1960920625736549 1109 52-53 0.995044652564836 1109 54-55 0.19902850770898084 1109 56-57 0.10790363411152981 1109 58-59 0.2775095492604933 1109 60-61 0.5500751442270015 1109 62-63 0.8121015717000262 1109 64-65 0.4765029513300405 1109 66-67 0.8979381375021163 1109 68-69 0.9549067314711692 1109 70-71 0.6501511811836878 1109 72-73 0.7499968723718666 1109 74-75 1.0349403309784506 1109 76 -0.658538599220563 1110 1 -0.04690006760025511 1110 2 0.3316610470736059 1110 3 0.6595299323150527 1110 4 -0.04723833135560085 1110 5 -0.17813299162724405 1110 6 0.44523459410370947 1110 7 -0.24724028926600283 1110 8 0.14307426223712838 1110 9 0.27102854618428296 1110 10-11 -0.04684670895415621 1110 12-13 0.015118294253845477 1110 14-15 0.09947636408480776 1110 16-17 -0.08545913672857353 1110 18-19 0.04902876115652077 1110 20-21 0.14280484222424406 1110 22-23 0.4863087397937065 1110 24-25 0.7407807348982374 1110 26-27 0.21036805356181532 1110 28-29 0.25454475055281733 1110 30-31 0.2751670477964865 1110 32-33 0.7192980547452095 1110 34-35 0.16844747464164556 1110 36-37 0.133859676478977 1110 38-39 0.02666933862911236 1110 40-41 0.10425173624812345 1110 42-43 0.2330797498542836 1110 44-45 0.07479612961681426 1110 46-47 0.5792475683997012 1110 48-49 0.0769745217910014 1110 50-51 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-1.1179893263458034 2116 32-33 -1.428715668620626 2116 34-35 -0.49545646179749525 2116 36-37 -1.346259500119075 2116 38-39 -0.8159453237038576 2116 40-41 -0.7932022182480836 2116 42-43 -1.0346522833707041 2116 44-45 -0.9809099014413292 2116 46-47 -0.8738582459737216 2116 48-49 -0.8752103825065802 2116 50-51 -0.3832966407804932 2116 52-53 -0.8679581062561326 2116 54-55 -0.584186244723135 2116 56-57 -0.48466206938781653 2116 58-59 -1.1325383671036633 2116 60-61 -0.6297564224929033 2116 62-63 -0.18666421891997942 2116 64-65 -0.23904808685784928 2116 66-67 -0.6248584357518396 2116 68-69 -0.45652059537651724 2116 70-71 -0.7744531458091899 2116 72-73 -0.6155469057608443 2116 74-75 -0.7584384987006558 2116 76 -0.9762386137406729 2117 1 -0.15769315048635946 2117 2 -0.34991319264016596 2117 3 -0.14118563119120964 2117 4 -0.15898549891672076 2117 5 -0.9275665038514553 2117 6 -0.5261428656458449 2117 7 0.3551449224215375 2117 8 -0.13205990592029337 2117 9 0.06089139651224684 2117 10-11 -0.4685521353107518 2117 12-13 -0.1841661422398957 2117 14-15 -0.2007621570839433 2117 16-17 -0.2057334360726415 2117 18-19 0.4306030008702919 2117 20-21 -0.5815839472808264 2117 22-23 -0.8972332995622878 2117 24-25 -0.31318467953229856 2117 26-27 -0.19332902455386858 2117 28-29 -0.8685321725241124 2117 30-31 -0.29740301779683165 2117 32-33 -0.17980749087196557 2117 34-35 -0.6021547913094523 2117 36-37 -0.07758933962120551 2117 38-39 -0.21042070311209216 2117 40-41 0.027984592539112896 2117 42-43 -0.11176222987141671 2117 44-45 0.22923858638485228 2117 46-47 -0.13554074406302163 2117 48-49 -0.29261402919289026 2117 50-51 0.25836729633054745 2117 52-53 -0.6652886008516106 2117 54-55 -0.33407706249801095 2117 56-57 -0.7229522757422231 2117 58-59 -0.41073467674445396 2117 60-61 -0.6024062750969037 2117 62-63 -0.9148823650057949 2117 64-65 -0.5952823919129813 2117 66-67 0.1126947905829141 2117 68-69 -0.5898866833773866 2117 70-71 -0.10366485014372984 2117 72-73 -0.9661583262215956 2117 74-75 -0.2834685238125729 2117 76 -0.0763041230757544 2118 1 0.9968200540859691 2118 2 1.9129300605460102 2118 3 1.3077698280125745 2118 4 0.8483722727295806 2118 5 1.8179991248438547 2118 6 0.9365427210050541 2118 7 0.8671013013467785 2118 8 0.9856644404205284 2118 9 1.296799949921784 2118 10-11 0.21049792381419508 2118 12-13 1.166313426804912 2118 14-15 0.7765732784698542 2118 16-17 1.0584565521023706 2118 18-19 1.1536354538988007 2118 20-21 1.3078852420504035 2118 22-23 1.3968476359258162 2118 24-25 0.7475169365496939 2118 26-27 1.1319238988464662 2118 28-29 1.1512418387753272 2118 30-31 1.453089690125907 2118 32-33 1.370580106027262 2118 34-35 1.4221849800740713 2118 36-37 1.8260369906901914 2118 38-39 1.241228226069353 2118 40-41 1.544408189963903 2118 42-43 0.9733448519837964 2118 44-45 1.1743180765963857 2118 46-47 1.3730980681824079 2118 48-49 1.6409032484315986 2118 50-51 1.587102022384947 2118 52-53 0.9719798264247075 2118 54-55 1.3752909574730907 2118 56-57 1.1618526119203736 2118 58-59 1.2890419623305647 2118 60-61 1.543435270035154 2118 62-63 1.5489528734648417 2118 64-65 1.2574307863815903 2118 66-67 1.3549100054035748 2118 68-69 1.0399582778629224 2118 70-71 1.7032264913357125 2118 72-73 1.3851095754996052 2118 74-75 1.814251880864873 2118 76 2.4997699801958255 2119 1 0.981639508765408 2119 2 0.7301002889911103 2119 3 1.2040115376662222 2119 4 1.8522042550435067 2119 5 1.5306741432668467 2119 6 1.115245742375727 2119 7 0.6885456639112562 2119 8 0.18610659222598258 2119 9 1.3816194046012242 2119 10-11 1.0937330011907633 2119 12-13 1.4648395874534046 2119 14-15 0.9534340006511428 2119 16-17 0.6922443192357477 2119 18-19 0.444792417789742 2119 20-21 0.7005528912766366 2119 22-23 0.9864202225138854 2119 24-25 1.3443481819439427 2119 26-27 0.4365664928037276 2119 28-29 0.8561055086353164 2119 30-31 0.7830823209291466 2119 32-33 1.0966670625490025 2119 34-35 0.8659948547977265 2119 36-37 0.7194784055759627 2119 38-39 1.1098354478821761 2119 40-41 0.9427132747096607 2119 42-43 0.8321878880928608 2119 44-45 0.9357771775543853 2119 46-47 0.7579543688456312 2119 48-49 1.1379555697285753 2119 50-51 0.8129679030039583 2119 52-53 0.45270937690370516 2119 54-55 0.5630580908555487 2119 56-57 0.7578363996875055 2119 58-59 0.14106849143889377 2119 60-61 0.5342974660557829 2119 62-63 0.48966768555032303 2119 64-65 0.9269960037728922 2119 66-67 1.5914612213210333 2119 68-69 0.19865393003683351 2119 70-71 0.372607478808078 2119 72-73 0.6890152497811002 2119 74-75 1.0330801785804198 2119 76 0.7384582631729835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 5.0 13 150.0 14 637.0 15 1273.0 16 1655.0 17 1949.0 18 1826.0 19 1622.0 20 1268.0 21 888.0 22 657.0 23 549.0 24 453.0 25 469.0 26 506.0 27 536.0 28 603.0 29 756.0 30 935.0 31 1292.0 32 1595.0 33 2116.0 34 3135.0 35 4719.0 36 9554.0 37 29264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.86125241185757 18.466058586212945 11.100391744138454 29.572297257791032 2 20.57533766005964 17.992457463602875 29.64684558264632 31.78535929369117 3 20.214289890662457 18.96597088230135 27.593112319476116 33.226626907560075 4 16.65789627550722 19.48780915628837 26.46611705548734 37.38817751271707 5 17.61971583932643 29.2784891539496 30.816231070572414 22.285563936151554 6 31.232824650646084 27.642811202712974 15.764778109103666 25.359586037537273 7 29.316494182307196 25.698707829035843 17.50570075425364 27.479097234403323 8 25.27480558966263 27.802139975442902 17.11249488393849 29.810559550955972 9 23.707829035841666 16.05712448108519 17.992457463602875 42.242589019470266 10-11 28.079869028825357 20.977313921534233 21.90112845699585 29.041688592644565 12-13 26.034175290884644 18.35496696485997 22.30968251184003 33.30117523241537 14-15 25.719902941004502 19.65298485645793 20.3465766239841 34.280535578553476 16-17 27.316844997953577 20.91153598783839 20.873530959480792 30.89808805472724 18-19 26.554551833011754 20.073963632111326 21.324475238262295 32.04700929661463 20-21 25.987399871367593 20.40285330059054 21.855814769338714 31.75393205870315 22-23 28.061597380576508 20.49932760334444 20.50298193299421 30.936093083084838 24-25 26.526778927673504 21.697216862538735 20.023533882944513 31.752470326843245 26-27 25.921621937671752 21.900397591065897 21.684792141729524 30.493188329532835 28-29 26.991609659124133 21.089136408817165 22.754048997251942 29.16520493480676 30-31 26.601327252528794 20.312225925276266 22.548675670934923 30.537771151260014 32-33 25.525492603636792 21.44068292112495 22.76866631585102 30.26515815938724 34-35 25.893118166403557 21.535695492018945 22.409080278313745 30.162106063263757 36-37 26.26220546103023 20.167514471145413 22.691194527275915 30.87908554054844 38-39 25.221452376776003 20.16678360521546 24.68938197976963 29.922382038238904 40-41 25.28942290826171 19.84081740045606 24.55709524644799 30.31266444483424 42-43 24.31737122142314 19.891978015552827 24.33418113781208 31.456469625211952 44-45 23.65447582295504 19.437379407121558 26.004209787756533 30.90393498216687 46-47 23.794071215576214 20.528562240542595 25.54522598374554 30.13214056013565 48-49 23.902239373209376 20.65573291235456 24.99853826814009 30.443489446295974 50-51 23.399403613401155 22.04291644740689 24.04402736362042 30.513652575571538 52-53 22.977693971817807 21.61535987838391 24.149272057533764 31.257674092264516 54-55 23.127521487458342 20.891802607729637 23.895661579839793 32.08501432497223 56-57 23.097555984330235 21.44872244635444 24.003098871543006 31.450622697772317 58-59 23.481991463485937 21.12641057124481 23.453487692217738 31.938110273051514 60-61 23.291235455767996 20.462053440916797 23.90004677541952 32.34666432789569 62-63 23.30000584692744 21.195842834590422 23.402327077120972 32.101824241361165 64-65 24.04475822955037 21.143220487633748 22.413465473893467 32.39855580892241 66-67 23.727562415950416 21.859469098988484 20.806291293925042 33.60667719113606 68-69 23.564579313570718 22.50190025141788 21.050400514529617 32.88311992048178 70-71 25.143980588200897 22.29067999766123 20.962696602935157 31.602642811202713 72-73 24.76977723206455 22.030491726597674 20.690083611062388 32.50964743027539 74-75 23.631088113196515 23.346050400514528 20.63673039817576 32.386131088113196 76 23.541191603812198 21.950827340232706 20.154358884406246 34.35362217154886 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 2.0 17 4.0 18 4.5 19 4.5 20 5.0 21 6.0 22 7.5 23 19.0 24 35.0 25 41.0 26 64.0 27 108.0 28 147.5 29 166.0 30 209.0 31 308.0 32 461.5 33 559.0 34 654.0 35 838.5 36 1048.5 37 1169.0 38 1333.5 39 1728.0 40 2108.0 41 2371.0 42 2484.0 43 2567.5 44 2735.5 45 2825.5 46 2831.0 47 2849.0 48 2787.0 49 2570.0 50 2433.0 51 2306.5 52 2036.0 53 1757.0 54 1622.0 55 1502.0 56 1322.5 57 1194.5 58 1126.0 59 1156.0 60 1236.0 61 1322.0 62 1358.0 63 1437.5 64 1624.5 65 1795.5 66 1927.0 67 1995.0 68 2057.5 69 2133.0 70 2238.0 71 2330.0 72 2307.5 73 2235.5 74 2038.5 75 1891.0 76 1856.5 77 1613.0 78 1238.0 79 1072.0 80 894.5 81 595.5 82 365.5 83 257.0 84 197.0 85 95.0 86 42.5 87 32.0 88 25.0 89 13.5 90 9.5 91 8.0 92 6.0 93 4.5 94 4.0 95 4.0 96 3.0 97 2.0 98 1.0 99 11.5 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 68412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.35941062971409 #Duplication Level Percentage of deduplicated Percentage of total 1 82.20295181760754 62.76968952815296 2 11.556499933000248 17.648950476524586 3 3.3959302436876664 7.779336958428345 4 1.2653381573154157 3.8648190375957436 5 0.6719117900419227 2.5653394141378705 6 0.34648442734355556 1.5874407998596736 7 0.19525641761902027 1.0436765479740395 8 0.13017094507934684 0.7951821317897446 9 0.09954248741361818 0.6840905104367655 >10 0.13591378089167097 1.2614745951002748 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007308659299538093 0.0 2 0.0 0.0 0.0 0.01023212301935333 0.0 3 0.0 0.0 0.0 0.01023212301935333 0.0 4 0.0 0.0 0.0 0.016079050458983803 0.0 5 0.0 0.0 0.0 0.016079050458983803 0.0 6 0.0 0.0 0.0 0.01754078231889142 0.0 7 0.0 0.0 0.0 0.01900251417879904 0.0 8 0.0 0.0 0.0 0.02046424603870666 0.0 9 0.0 0.0 0.0 0.023387709758521898 0.0 10 0.0 0.0 0.0 0.027772905338244752 0.0 11 0.0 0.0 0.0 0.02923463719815237 0.0 12 0.0 0.0 0.0 0.02923463719815237 0.0 13 0.0 0.0 0.0 0.02923463719815237 0.0 14 0.0 0.0 0.0 0.03215810091796761 0.0 15 0.0 0.0 0.0 0.03215810091796761 0.0 16 0.0 0.0 0.0 0.03215810091796761 0.0 17 0.0 0.0 0.0 0.03215810091796761 0.0 18 0.0 0.0 0.0 0.0394667602175057 0.0 19 0.0 0.0 0.0 0.04385195579722856 0.0 20 0.0 0.0 0.0 0.04531368765713618 0.0 21 0.0 0.0 0.0 0.048237151376951413 0.0 22 0.0 0.0 0.0 0.05262234695667427 0.0 23 0.0 0.0 0.0 0.05262234695667427 0.0 24 0.0 0.0 0.0 0.06139273811611998 0.0 25 0.0 0.0 0.0 0.06577793369584284 0.0 26 0.0 0.0 0.0 0.07162486113547331 0.0 27 0.0 0.0 0.0 0.12863240367187043 0.0 28 0.0 0.0 0.0 0.17540782318891424 0.0 29 0.0 0.0 0.0 0.2441092206045723 0.0 30 0.0 0.0 0.0 0.3288896684792142 0.0 31 0.0 0.0 0.0 0.42390223937320937 0.0 32 0.0 0.0 0.0 0.548149447465357 0.0 33 0.0 0.0 0.0 0.6957843653160264 0.0 34 0.0 0.0 0.0 0.8945798982634625 0.0 35 0.0 0.0 0.0 1.2015435888440624 0.0 36 0.0 0.0 0.0 1.6327544875168098 0.0 37 0.0 0.0 0.0 2.115126001286324 0.0 38 0.0 0.0 0.0 2.670584108051219 0.0 39 0.0 0.0 0.0 3.169034672279717 0.0 40 0.0 0.0 0.0 3.728877974624335 0.0 41 0.0 0.0 0.0 4.328188037186458 0.0 42 0.0 0.0 0.0 5.045898380401099 0.0 43 0.0 0.0 0.0 5.738759281997311 0.0 44 0.0 0.0 0.0 6.424311524293984 0.0 45 0.0 0.0 0.0 7.045547564754721 0.0 46 0.0 0.0 0.0 7.725252879611764 0.0 47 0.0 0.0 0.0 8.342103724492778 0.0 48 0.0 0.0 0.0 8.871250657779337 0.0 49 0.0 0.0 0.0 9.409167982225341 0.0 50 0.0 0.0 0.0 9.933929719932175 0.0 51 0.0 0.0 0.0 10.432380284160674 0.0 52 0.0 0.0 0.0 11.065310179500672 0.0 53 0.0 0.0 0.0 11.761094544816698 0.0 54 0.0 0.0 0.0 12.399871367596328 0.0 55 0.0 0.0 0.0 12.959714669940945 0.0 56 0.0 0.0 0.0 13.487399871367597 0.0 57 0.0 0.0 0.0 13.99462082675554 0.0 58 0.0 0.0 0.0 14.490147927264223 0.0 59 0.0 0.0 0.0 14.896509384318541 0.0 60 0.0 0.0014617318599076186 0.0 15.34526106531018 0.0 61 0.0 0.0014617318599076186 0.0 15.81155352862071 0.0 62 0.0 0.0014617318599076186 0.0 16.305618897269486 0.0 63 0.0 0.0014617318599076186 0.0 16.81283985265743 0.0 64 0.0 0.0014617318599076186 0.0 17.3639127638426 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGTAT 20 0.006924257 52.5 1 AGTCAAT 20 0.006924257 52.5 33 AGGAGCG 20 0.006924257 52.5 11 TTATCGC 20 0.006924257 52.5 17 TCAGGAT 20 0.006924257 52.5 19 GCTCTTC 20 0.006924257 52.5 1 TTGAGAT 20 0.006924257 52.5 17 GATGGGA 20 0.006924257 52.5 24 ATGAAAG 20 0.006924257 52.5 69 ATTGAGA 20 0.006924257 52.5 16 GTAAAAC 20 0.006924257 52.5 66 TCACGAA 30 5.8404944E-4 46.666668 36 TCTTCCG 40 4.549419E-5 43.75 17 CTTCCGA 50 3.4329532E-6 42.0 18 TCCCTGG 40 0.0024036146 35.0 2 TAGATCT 70 3.4435783E-5 30.0 39 CATCCTA 60 4.936028E-4 29.166668 6 TTCCGAC 50 0.0071637975 27.999998 19 ACCTCTG 50 0.0071637975 27.999998 8 TCTCGGT 50 0.0071637975 27.999998 43 >>END_MODULE