FastQCFastQC Report
Wed 25 May 2016
SRR1780192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68412
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT51557.535227737823774No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG15132.2116003040402266RNA PCR Primer, Index 34 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG12961.8944044904402737No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC11111.6239840963573644No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3830.5598433023446179No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT2530.36981816055662753RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT1740.25434134362392563No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT1320.19294860550780565No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1220.17833128690872946No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1130.1651757001695609No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1000.14617318599076187No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC720.10524469391334855No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGC690.10085949833362569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCTT150.002215944770.0000127
CTTTATA650.064.615392
TCTTTAT600.064.166671
TTAATAC200.00692425752.53
ATTCGAG200.00692425752.548
AAGCGAG200.00692425752.515
AGTGCTA200.00692425752.553
GAATTAT200.00692425752.543
AGTAAGG200.00692425752.52
TGCGTCG200.00692425752.545
GAGAAGA305.8404944E-446.66666838
TTTATAC900.046.6666643
TAGCACC551.2885175E-744.54545233
AGCACCT602.5522786E-740.83333634
ACAACAA350.001247508340.070
GTGTGGG350.001247508340.047
GTATAGG350.001247508340.01
TGCTATA350.001247508340.055
GCACCTC654.781359E-737.6923135
CTCGTAT12700.035.039