##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780192_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68412 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.549450388820674 34.0 34.0 34.0 34.0 34.0 2 33.612772612991876 34.0 34.0 34.0 34.0 34.0 3 33.64095480325089 34.0 34.0 34.0 34.0 34.0 4 33.67137344325557 34.0 34.0 34.0 34.0 34.0 5 33.64956440390575 34.0 34.0 34.0 34.0 34.0 6 37.29794480500497 38.0 38.0 38.0 36.0 38.0 7 37.29006607028007 38.0 38.0 38.0 36.0 38.0 8 37.159898848155294 38.0 38.0 38.0 36.0 38.0 9 37.20477401625446 38.0 38.0 38.0 36.0 38.0 10-11 37.127806525171025 38.0 38.0 38.0 36.0 38.0 12-13 37.089034087586974 38.0 38.0 38.0 36.0 38.0 14-15 37.00994708530667 38.0 38.0 38.0 35.0 38.0 16-17 37.039145179208326 38.0 38.0 38.0 35.5 38.0 18-19 36.9619657370052 38.0 37.5 38.0 35.0 38.0 20-21 37.00563497631994 38.0 38.0 38.0 35.0 38.0 22-23 36.998194761153016 38.0 38.0 38.0 35.0 38.0 24-25 36.97555253464304 38.0 37.5 38.0 35.0 38.0 26-27 36.8132637548968 38.0 37.0 38.0 34.5 38.0 28-29 36.774805589662634 38.0 37.0 38.0 34.0 38.0 30-31 36.720801906098345 38.0 37.0 38.0 34.0 38.0 32-33 36.64905279775478 38.0 37.0 38.0 34.0 38.0 34-35 36.731896450915045 38.0 37.0 38.0 34.0 38.0 36-37 36.74104689235807 38.0 37.0 38.0 34.0 38.0 38-39 35.85868707244343 38.0 37.0 38.0 31.5 38.0 40-41 36.25860960065486 38.0 37.0 38.0 34.0 38.0 42-43 36.568789101327255 38.0 37.0 38.0 34.0 38.0 44-45 36.682489914050166 38.0 37.0 38.0 34.0 38.0 46-47 36.585854820791674 38.0 37.0 38.0 34.0 38.0 48-49 36.60082295503713 38.0 37.0 38.0 34.0 38.0 50-51 36.33046833888791 38.0 37.0 38.0 34.0 38.0 52-53 36.352006957843656 38.0 37.0 38.0 34.0 38.0 54-55 36.32666052739285 38.0 37.0 38.0 34.0 38.0 56-57 36.397379114775184 38.0 37.0 38.0 34.0 38.0 58-59 36.2167456001871 38.0 37.0 38.0 34.0 38.0 60-61 36.207595158744084 38.0 37.0 38.0 34.0 38.0 62-63 36.19481962228849 38.0 37.0 38.0 34.0 38.0 64-65 36.12053440916799 38.0 37.0 38.0 33.0 38.0 66-67 35.68697012220078 38.0 36.5 38.0 31.0 38.0 68-69 35.56881102730515 38.0 37.0 38.0 31.0 38.0 70-71 34.67450885809507 38.0 35.0 38.0 25.5 38.0 72-73 33.4067122727007 38.0 34.0 38.0 20.0 38.0 74-75 33.03554201017366 38.0 34.0 38.0 11.0 38.0 76 32.1818979126469 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2602876828535372 1101 2 0.05285363697309009 1101 3 0.11026376758952239 1101 4 0.242553717350944 1101 5 -0.00705213697913365 1101 6 -0.025715554165358867 1101 7 0.05696775449312952 1101 8 -0.10809254296331972 1101 9 0.033034967693204464 1101 10-11 0.3004083813924936 1101 12-13 0.39797505339954853 1101 14-15 0.3259061083478869 1101 16-17 0.19451055227541048 1101 18-19 0.14379516638803125 1101 20-21 0.19775994208991676 1101 22-23 0.2996037166876988 1101 24-25 -0.07789606570293728 1101 26-27 0.11259291529844973 1101 28-29 0.10092983375555775 1101 30-31 -0.0051201951809360935 1101 32-33 -0.05005284855334935 1101 34-35 0.058741791908488494 1101 36-37 0.06661013179982689 1101 38-39 0.25460544074673663 1101 40-41 0.30540661989190454 1101 42-43 -0.06353708580158468 1101 44-45 -0.028493924561878714 1101 46-47 0.09790436313761575 1101 48-49 0.24017276015302258 1101 50-51 0.21043211720574817 1101 52-53 0.22477152821747381 1101 54-55 0.6056741007408775 1101 56-57 0.06470653217296274 1101 58-59 0.017321479697145037 1101 60-61 -0.09779806991222983 1101 62-63 -0.2593565785702623 1101 64-65 -0.18848130015230424 1101 66-67 -0.49061477491013505 1101 68-69 -0.5716084285767948 1101 70-71 -0.6193775135250803 1101 72-73 -0.4883727874314303 1101 74-75 -0.34584707819450244 1101 76 -0.816153979099834 1102 1 0.1208196742266523 1102 2 0.011157015837220285 1102 3 0.22075082725882567 1102 4 0.09504293374202888 1102 5 -0.029113603549944855 1102 6 0.32255008206756486 1102 7 -0.04754338856941587 1102 8 0.005899189603177035 1102 9 0.02643000723310962 1102 10-11 0.03301981201794746 1102 12-13 -0.09935151022374811 1102 14-15 0.1142777834018105 1102 16-17 -0.0938611227785131 1102 18-19 0.09257302404438406 1102 20-21 -0.14313689471812552 1102 22-23 0.1821747087797263 1102 24-25 -0.050622521490133465 1102 26-27 0.3172118944501392 1102 28-29 0.3483013290826591 1102 30-31 0.26871607369037065 1102 32-33 0.4220220975286111 1102 34-35 0.12535304280712012 1102 36-37 0.29284126048422365 1102 38-39 0.3834246355705986 1102 40-41 0.6853715731629322 1102 42-43 0.6384084569733943 1102 44-45 0.46013835437413064 1102 46-47 0.22416245084430386 1102 48-49 0.383347634344247 1102 50-51 0.16905001799654684 1102 52-53 0.42916584885011844 1102 54-55 0.46256662410536364 1102 56-57 0.5027232467667844 1102 58-59 0.7997846357000213 1102 60-61 0.5453992701309147 1102 62-63 0.033867720495159404 1102 64-65 0.45853523471470226 1102 66-67 0.625016964845436 1102 68-69 0.5980986167143598 1102 70-71 0.6000015303282566 1102 72-73 0.38256089337374277 1102 74-75 0.5644018434446068 1102 76 0.4772500467808243 1103 1 -0.17413273543785124 1103 2 0.053385363311271306 1103 3 0.04789669917406769 1103 4 -0.040169607711085575 1103 5 0.14694910371565584 1103 6 -0.017451640659508882 1103 7 0.06365864424854095 1103 8 -0.0339388740515858 1103 9 0.30437048701259783 1103 10-11 0.04081515204119768 1103 12-13 0.07358875421486033 1103 14-15 0.09234531430322335 1103 16-17 0.04327871959196017 1103 18-19 0.1636462830133354 1103 20-21 0.01376047381626222 1103 22-23 0.18925942388372619 1103 24-25 0.02005235684226392 1103 26-27 0.04776661256892112 1103 28-29 -0.14374182523062018 1103 30-31 -0.23368497717314085 1103 32-33 0.008869076295994205 1103 34-35 -0.08696230238431468 1103 36-37 -0.12638437369468392 1103 38-39 0.19631494092397617 1103 40-41 -0.16593554600671467 1103 42-43 0.039340883003802674 1103 44-45 -0.4254941018039986 1103 46-47 -0.19689358085386743 1103 48-49 0.2803070210534102 1103 50-51 0.259816200864023 1103 52-53 -0.0857788085425355 1103 54-55 -0.17744801907478802 1103 56-57 -0.18827335084724695 1103 58-59 0.3717946275170618 1103 60-61 0.44467357134973895 1103 62-63 -0.08366533433063239 1103 64-65 0.10963993345280443 1103 66-67 -0.25044063453437815 1103 68-69 0.011230103858508755 1103 70-71 -0.2568398495761315 1103 72-73 0.1813033026075601 1103 74-75 -5.018991799730088E-4 1103 76 0.47040220664990073 1104 1 -0.1136146705008727 1104 2 0.05197551965661518 1104 3 0.09206478620560432 1104 4 0.00416681886130732 1104 5 0.13987884299979214 1104 6 -0.019766309346252342 1104 7 -0.1500484028654938 1104 8 0.16994135236509322 1104 9 0.22211557886286215 1104 10-11 0.25213388191936303 1104 12-13 0.07209474078977962 1104 14-15 0.4433542994262183 1104 16-17 0.11130893653954388 1104 18-19 0.04632414425947218 1104 20-21 0.16924308223744333 1104 22-23 0.20148508303824997 1104 24-25 0.1482850020877251 1104 26-27 0.062496322393634784 1104 28-29 -0.18117633062800564 1104 30-31 0.21147841739369966 1104 32-33 0.23591703202269798 1104 34-35 0.01673485478168857 1104 36-37 0.01679892702286878 1104 38-39 0.3468736177751808 1104 40-41 0.34949908564532706 1104 42-43 0.14831969326409933 1104 44-45 -0.057230313743474426 1104 46-47 -0.20254347669378348 1104 48-49 0.039665647402756576 1104 50-51 0.09063496230585599 1104 52-53 -0.09018720026864457 1104 54-55 -0.45967978053766245 1104 56-57 -0.5455740263096445 1104 58-59 0.28019687484993483 1104 60-61 -0.28608837549704447 1104 62-63 -0.020201327428708282 1104 64-65 0.025428078140642185 1104 66-67 -0.28650738116233043 1104 68-69 -0.2584498405894422 1104 70-71 -0.7640363649476356 1104 72-73 -0.024386573873300676 1104 74-75 0.44960646939944837 1104 76 -0.008944635931584344 1105 1 -0.016598937634981326 1105 2 0.019860563695658584 1105 3 0.16155341396210332 1105 4 -0.05973848097205803 1105 5 0.2921868349494119 1105 6 0.20302578743143584 1105 7 0.11771328857989971 1105 8 0.3122729327931495 1105 9 0.19082297352623812 1105 10-11 0.20746725853431514 1105 12-13 0.24411750691758982 1105 14-15 0.058634872474755184 1105 16-17 0.3172320251153664 1105 18-19 0.30146924586306056 1105 20-21 0.13152315754744848 1105 22-23 0.127495167653791 1105 24-25 0.33877819605427106 1105 26-27 0.3739399805554626 1105 28-29 0.54154002332902 1105 30-31 0.37308113547892674 1105 32-33 0.08152070230337927 1105 34-35 -0.09705250265950838 1105 36-37 0.26664203118006213 1105 38-39 0.213295743335145 1105 40-41 0.14856420283029337 1105 42-43 0.17631207570042307 1105 44-45 -0.07691262671280441 1105 46-47 -0.08451452129548187 1105 48-49 0.08884437881872032 1105 50-51 -0.18259103263019938 1105 52-53 0.23837433536293418 1105 54-55 0.021254669461249875 1105 56-57 -0.17051721919415996 1105 58-59 0.3015563502767975 1105 60-61 0.5328754627162837 1105 62-63 0.7400510043681265 1105 64-65 0.0685365635006292 1105 66-67 -0.5812659779724711 1105 68-69 0.48364585505426305 1105 70-71 0.05703745184129616 1105 72-73 0.4449916857730898 1105 74-75 -0.05605260134434076 1105 76 -0.0796884304304939 1106 1 0.16072934645309545 1106 2 0.15020365537574065 1106 3 -0.156367627331349 1106 4 -0.034613194684389725 1106 5 0.11916703858214817 1106 6 0.2458385496255886 1106 7 0.12685181779824717 1106 8 -0.05594403357797262 1106 9 -0.1690491324172072 1106 10-11 0.22925913590114533 1106 12-13 0.2832000257955656 1106 14-15 0.025436840773359393 1106 16-17 0.026080298318838402 1106 18-19 -0.03360637943457334 1106 20-21 0.13240753134277838 1106 22-23 -0.15863376951031682 1106 24-25 0.07588107820578927 1106 26-27 -0.2675598266963988 1106 28-29 0.04904586246368581 1106 30-31 0.08945792406823472 1106 32-33 0.4878451087525093 1106 34-35 -0.18188435825713611 1106 36-37 0.21606265296286864 1106 38-39 -0.2983711878877884 1106 40-41 0.27654896438951226 1106 42-43 -0.2332210202881484 1106 44-45 0.12905576259306883 1106 46-47 0.14975382946077076 1106 48-49 0.1669187115700268 1106 50-51 0.28231746869636254 1106 52-53 0.29633483333341815 1106 54-55 0.03420280887484495 1106 56-57 -0.19068774460289006 1106 58-59 0.16719956214100762 1106 60-61 0.28987675526259693 1106 62-63 0.7532032300421747 1106 64-65 0.05351354710570888 1106 66-67 0.3768056336286989 1106 68-69 0.43103695235805617 1106 70-71 0.20394554499534223 1106 72-73 0.30658718680485464 1106 74-75 -0.05932931963727839 1106 76 0.010631077948382028 1107 1 0.028785851351706526 1107 2 0.10853129265074557 1107 3 -0.02998348515772875 1107 4 -0.06152137422414228 1107 5 -0.13200634943334677 1107 6 0.11956832861852718 1107 7 0.181784604309982 1107 8 0.19094591857513876 1107 9 0.06888661604485691 1107 10-11 0.11831131179543064 1107 12-13 0.07189904607353981 1107 14-15 0.22306728050906344 1107 16-17 0.27353985630470845 1107 18-19 0.03353875613161961 1107 20-21 0.3391060959360743 1107 22-23 -0.038697565363584374 1107 24-25 -0.1218063221131942 1107 26-27 0.30114602885156216 1107 28-29 0.20241792899365407 1107 30-31 0.0355162517055021 1107 32-33 0.24442975217925778 1107 34-35 0.03454307395976741 1107 36-37 0.23313943582913055 1107 38-39 0.3824900561313527 1107 40-41 0.3789185246538054 1107 42-43 0.27966346364896566 1107 44-45 0.14462878606082796 1107 46-47 -0.006033365800107049 1107 48-49 -0.01039958416932052 1107 50-51 0.42494676922039787 1107 52-53 0.3243594403053862 1107 54-55 0.2491264816932599 1107 56-57 0.02063601935245174 1107 58-59 0.5132692818949423 1107 60-61 0.16671658210242413 1107 62-63 0.07040532619163997 1107 64-65 0.42415606248506066 1107 66-67 0.2669172763719203 1107 68-69 0.13127618680782405 1107 70-71 0.5670464791489138 1107 72-73 0.813023732715088 1107 74-75 1.0958882697908408 1107 76 1.296208658262806 1108 1 -0.332404624838766 1108 2 0.16900748312693992 1108 3 -0.025697770872014303 1108 4 -0.09033089803367034 1108 5 -0.002244444671440249 1108 6 -0.33090786185766774 1108 7 -0.34226301473763954 1108 8 0.10094591857514246 1108 9 -0.027541955383718175 1108 10-11 -0.1772839262998147 1108 12-13 -0.09750571583122536 1108 14-15 0.11032918527096314 1108 16-17 -0.01991252464767257 1108 18-19 0.12437208946494849 1108 20-21 0.07160609593607603 1108 22-23 -0.24703089869691297 1108 24-25 -0.020972988779853097 1108 26-27 -0.2543301616246296 1108 28-29 0.19717983375555548 1108 30-31 -0.1407932721040197 1108 32-33 -0.32687977163026716 1108 34-35 -0.3646235927068986 1108 36-37 -0.27281294512325616 1108 38-39 -0.5150099438686553 1108 40-41 0.19415661989190625 1108 42-43 -0.004979393493890427 1108 44-45 -0.07060930917726438 1108 46-47 0.19194282467608303 1108 48-49 0.09507660630686843 1108 50-51 0.11947057874421319 1108 52-53 -0.240997702551752 1108 54-55 -0.5293258992591277 1108 56-57 -0.37519731398088396 1108 58-59 -0.080659289533628 1108 60-61 -0.15625960837375885 1108 62-63 -0.0818565785702674 1108 64-65 -0.18405822322922916 1108 66-67 -0.45974939029474626 1108 68-69 -0.19872381319218135 1108 70-71 -0.1773582827558542 1108 72-73 -1.0442381720468177 1108 74-75 0.03636446026703055 1108 76 0.07692294397708821 1109 1 0.11754382876947744 1109 2 0.05364665838467175 1109 3 0.06986923943725998 1109 4 0.15487528753334345 1109 5 0.23873493677185564 1109 6 -0.049258377321585556 1109 7 0.1318091502108132 1109 8 -0.023487071115582125 1109 9 -0.027284223424949516 1109 10-11 -0.2038045448565171 1109 12-13 -0.035882004491021746 1109 14-15 0.25205598939467677 1109 16-17 0.2967111866925407 1109 18-19 0.5050164193618585 1109 20-21 0.46178650830721324 1109 22-23 -0.0596597646762973 1109 24-25 0.13369195967374736 1109 26-27 -0.04293840904730928 1109 28-29 0.31388086468339793 1109 30-31 -0.21252007622773306 1109 32-33 0.05876455826663829 1109 34-35 -0.20635039683060796 1109 36-37 0.22486746724788276 1109 38-39 -0.08555118098205838 1109 40-41 0.29219785700531276 1109 42-43 0.14262369928961505 1109 44-45 0.4870195568021174 1109 46-47 0.4009376700369103 1109 48-49 0.2677054722862522 1109 50-51 -0.13150880269908782 1109 52-53 0.18407961703587716 1109 54-55 -0.13337229101170323 1109 56-57 0.1744160880809673 1109 58-59 0.4784386486107053 1109 60-61 0.2716012163685093 1109 62-63 -0.0828101868176816 1109 64-65 0.10751394171923323 1109 66-67 0.16880731073617739 1109 68-69 0.37045144453978196 1109 70-71 0.571121098687442 1109 72-73 -0.14070724421176806 1109 74-75 0.6136840478958945 1109 76 0.1417167584100767 1110 1 0.05259537516123203 1110 2 -0.06253097841152311 1110 3 0.26122530605105965 1110 4 0.24287423017145926 1110 5 -0.0016034190304168305 1110 6 0.2316562407064353 1110 7 0.21145493398031334 1110 8 0.031971559600783905 1110 9 0.3923298394880774 1110 10-11 0.22252376600788182 1110 12-13 0.48178915596364646 1110 14-15 0.004752262194038792 1110 16-17 0.13874132150618124 1110 18-19 0.27456439715726333 1110 20-21 0.22372148055146113 1110 22-23 0.15361012694410903 1110 24-25 0.5307577804509123 1110 26-27 0.1375929152984412 1110 28-29 0.23153880811453575 1110 30-31 -0.071626605437352 1110 32-33 0.16805612580562723 1110 34-35 0.12428666370335861 1110 36-37 0.28423833692803413 1110 38-39 0.11069518433647829 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2116 4 -0.3825139966252209 2116 5 -0.275131768615104 2116 6 -0.03851349566048157 2116 7 0.05006092892433145 2116 8 -0.0329977433966917 2116 9 -0.27296449059497974 2116 10-11 -0.161333222074461 2116 12-13 -0.30831557498615325 2116 14-15 -0.39118489923607314 2116 16-17 -0.9722012570420375 2116 18-19 -0.7993250936336409 2116 20-21 -0.18976714350054635 2116 22-23 -0.3840027296828339 2116 24-25 -0.29762791835732116 2116 26-27 -0.43440058415983884 2116 28-29 -0.20760889863881005 2116 30-31 -0.5305467932307764 2116 32-33 -0.06952061670069298 2116 34-35 -0.20747570538295435 2116 36-37 -0.3738692831514214 2116 38-39 -0.20131276077005822 2116 40-41 -1.3561250702489431 2116 42-43 -0.42536671743755505 2116 44-45 -0.27666564720543363 2116 46-47 -0.23809238659151788 2116 48-49 -0.6825994500311623 2116 50-51 -0.9732759001290319 2116 52-53 -0.37835685748133585 2116 54-55 -0.5466146316534903 2116 56-57 -0.9324156238400363 2116 58-59 -0.8447790078434849 2116 60-61 -0.86601312950053 2116 62-63 -0.37027207152800656 2116 64-65 -0.6960652654827442 2116 66-67 -0.2596085452243315 2116 68-69 -0.11379423572739 2116 70-71 -0.6258441982488137 2116 72-73 -0.5776536650045614 2116 74-75 -0.23124117353578555 2116 76 -1.0532883236285429 2117 1 -0.3225984232883832 2117 2 -0.2521553075707388 2117 3 -0.7474807166084503 2117 4 -0.44502082051428715 2117 5 -0.6892599485474094 2117 6 -0.12362104015223707 2117 7 -0.1667203790787255 2117 8 -0.6974261744481183 2117 9 -0.4351000949185959 2117 10-11 -0.08528780226880173 2117 12-13 -0.2527964135056422 2117 14-15 -0.24348864418639948 2117 16-17 -0.12756756340736075 2117 18-19 -0.21994961596141138 2117 20-21 -0.6004287877848498 2117 22-23 -0.14644950334808016 2117 24-25 -0.42296911281086835 2117 26-27 -0.36646194457036074 2117 28-29 -1.6149131895002569 2117 30-31 -0.4630994736544096 2117 32-33 -0.4898448879093422 2117 34-35 -0.39103832138906824 2117 36-37 -0.9923865885341101 2117 38-39 -0.13533940123299004 2117 40-41 -1.0087891165422036 2117 42-43 -0.442208075664432 2117 44-45 -0.1858806270067248 2117 46-47 -0.22059593501384 2117 48-49 -0.6706985874915787 2117 50-51 -0.8159170181550195 2117 52-53 -0.7797380126292666 2117 54-55 -0.3977173721273388 2117 56-57 -0.9987438256087913 2117 58-59 -0.5225003748049488 2117 60-61 -0.996240228528805 2117 62-63 -0.7118759584152201 2117 64-65 -0.6619458201284516 2117 66-67 -0.34703621200017665 2117 68-69 -0.8210493945875328 2117 70-71 -0.18826913546902801 2117 72-73 -1.0742963115817048 2117 74-75 -0.04890685756242874 2117 76 -1.1266041878058566 2118 1 -0.1236758112794476 2118 2 -0.32980607619509783 2118 3 -0.6665452284991389 2118 4 -0.0412631014235032 2118 5 -0.03402410568838832 2118 6 -0.008195997450883397 2118 7 0.1542624089911797 2118 8 -0.09041001362825085 2118 9 -0.575423311315916 2118 10-11 -0.28588562121506556 2118 12-13 0.15151970789759162 2118 14-15 0.10410037171164532 2118 16-17 0.115468831284538 2118 18-19 0.058397513193767736 2118 20-21 -0.16004644643680876 2118 22-23 -0.5313529325952189 2118 24-25 -0.3579645142035872 2118 26-27 0.0377884824431618 2118 28-29 0.13006118968776548 2118 30-31 -0.1620220856633381 2118 32-33 -0.6802272292573832 2118 34-35 -0.3211066435543586 2118 36-37 0.18354298708013772 2118 38-39 0.21638836121609728 2118 40-41 0.47330916226478337 2118 42-43 -0.39612346129050025 2118 44-45 -0.15993134307557 2118 46-47 -0.1291164973578134 2118 48-49 0.08685626732381735 2118 50-51 -0.15959721786596504 2118 52-53 -0.3820570245856487 2118 54-55 0.13851308379172877 2118 56-57 0.2762857368665763 2118 58-59 -0.1539220013980369 2118 60-61 -0.10342062532291152 2118 62-63 0.2639485061755025 2118 64-65 -0.07342263000888494 2118 66-67 -0.12347820385407005 2118 68-69 -0.671266186073538 2118 70-71 -0.09994302851856673 2118 72-73 0.433685556766747 2118 74-75 0.027889883995847242 2118 76 -0.5096024797517238 2119 1 -1.1213176683170332 2119 2 -0.11403599513393203 2119 3 -0.48526298826332237 2119 4 -0.32880915890323337 2119 5 -0.38376618380187466 2119 6 -0.5839513401185386 2119 7 -0.4503064929985072 2119 8 -0.8438366901205114 2119 9 -0.43732456407936837 2119 10-11 -0.9958708828215563 2119 12-13 -0.8465274549616595 2119 14-15 -1.8460838582072938 2119 16-17 -0.9813255681259321 2119 18-19 -0.9374757366220052 2119 20-21 -0.7652417301508763 2119 22-23 -0.6981178552186549 2119 24-25 -0.6862990757363789 2119 26-27 -0.6741127703202778 2119 28-29 -0.6776027749400981 2119 30-31 -0.9339454460170629 2119 32-33 -0.9945971629346175 2119 34-35 -0.8147322883590675 2119 36-37 -1.302160771210204 2119 38-39 -0.5740316829990846 2119 40-41 -0.5214955540211363 2119 42-43 -0.9796533065373723 2119 44-45 -1.5006093091772712 2119 46-47 -0.9959919579326169 2119 48-49 -1.0525320893453056 2119 50-51 -1.1437902908210091 2119 52-53 -0.9054542242908781 2119 54-55 -0.8535650296939039 2119 56-57 -1.3213929661547965 2119 58-59 -1.601637550403197 2119 60-61 -1.1954987388085456 2119 62-63 -0.5300087524833046 2119 64-65 -0.8724277884466147 2119 66-67 -0.42583634681648874 2119 68-69 -1.727419465366097 2119 70-71 -0.19268436971237435 2119 72-73 -0.09391208509029525 2119 74-75 -0.2288529310373164 2119 76 -1.3398161864576927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 4.0 22 5.0 23 13.0 24 28.0 25 57.0 26 125.0 27 251.0 28 366.0 29 617.0 30 979.0 31 1434.0 32 2137.0 33 3457.0 34 5464.0 35 8072.0 36 10960.0 37 34441.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.471671636554994 14.618780330936094 12.0490557212185 36.860492311290415 2 20.079810559550957 17.134420861837103 35.43968894346021 27.346079635151728 3 20.19236391276384 15.946032859732211 31.29860258434193 32.56300064316201 4 16.646202420627958 22.80886394199848 28.361983277787523 32.18295035958604 5 17.698649359761447 27.51710226276092 36.95696661404432 17.827281763433316 6 31.59094895632345 33.900485294977486 15.272174472314799 19.23639127638426 7 29.32526457346664 22.38934689820499 19.379640998655205 28.90574752967316 8 24.653569549201894 34.95585569783079 14.319125299655031 26.071449453312283 9 22.61153014091095 15.087996257966438 14.903818043618077 47.39665555750453 10-11 28.421914284043737 24.486201251242473 21.234578728877977 25.857305735835816 12-13 26.691223761913115 16.26980646670175 24.03087177688125 33.008097994503885 14-15 23.593813950768872 15.821785651640063 18.377623808688533 42.206776588902535 16-17 30.51072911185172 22.785476232239958 16.36993509910542 30.3338595568029 18-19 33.20616266152137 20.74636028766883 16.691516108285096 29.355960942524707 20-21 26.726305326550897 18.97912646904052 17.13515172776706 37.159416476641525 22-23 26.524586329883647 22.96819271472841 16.614775185639946 33.892445769748 24-25 31.95784365316026 22.832982517686958 17.64821961059463 27.560954218558148 26-27 31.4462375021926 25.53937905630591 18.561802023036893 24.452581418464597 28-29 32.00534993860727 21.190726773080744 17.42676723381863 29.377156054493366 30-31 31.456469625211952 18.228527158977954 22.834444249546863 27.48055896626323 32-33 26.851283400573 23.314623165526516 24.08276325790797 25.751330175992514 34-35 28.73618663392387 24.361954043150323 21.55981406770742 25.34204525521838 36-37 28.331286908729464 22.38861603227504 18.69335789042858 30.58673916856692 38-39 23.72536981816056 20.981699117113955 24.784394550663627 30.508536514061863 40-41 24.68499678418991 16.934894462959715 25.64973981172894 32.73036894112144 42-43 30.42229433432731 16.412325323042744 24.584868151786235 28.58051219084371 44-45 24.28228965678536 22.33672455124832 27.487867625562767 25.893118166403557 46-47 28.618517219201312 18.208793778869204 24.394843009998247 28.77784599193124 48-49 28.15441735368064 16.69078524235514 20.83625679705315 34.31854060691107 50-51 25.67751271706718 17.389493071390984 25.24191662281471 31.691077588727122 52-53 22.136467286440975 17.19289013623341 33.434923697596915 27.235718879728704 54-55 22.789130561889728 18.250453136876573 27.64208033678302 31.318335964450682 56-57 26.79646845582646 19.87370636730398 22.2278255276852 31.101999649184354 58-59 23.04858796702333 19.974565865637608 28.85239431678653 28.124451850552536 60-61 27.385546395369232 24.21139566157984 22.383499970765364 26.01955797228556 62-63 22.57352511255335 33.22443430977022 18.324270595801906 25.877769981874525 64-65 21.830965327720282 34.8623048587967 17.405572121849968 25.90115769163305 66-67 21.74618487984564 34.355083903408755 17.282055779687774 26.616675437057825 68-69 21.355902473250307 34.472753318131325 17.748348242998304 26.422995965620068 70-71 22.266561421972753 32.535227737823774 18.075045313687657 27.123165526515812 72-73 22.4098111442437 29.439279658539437 18.993012921709642 29.157896275507223 74-75 22.261445360463078 28.857510378296205 19.87516809916389 29.005876162076827 76 22.903876512892474 28.78150032158101 19.553587089984216 28.7610360755423 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 2.5 19 4.0 20 2.5 21 2.0 22 4.0 23 6.5 24 18.0 25 29.0 26 40.0 27 68.5 28 104.5 29 123.0 30 153.5 31 207.5 32 323.5 33 416.0 34 461.0 35 603.5 36 792.5 37 884.0 38 989.5 39 1368.0 40 2890.0 41 4768.5 42 5398.0 43 4996.0 44 4183.0 45 3340.0 46 2908.0 47 2735.5 48 2363.0 49 1976.0 50 1789.0 51 1696.0 52 1409.5 53 1126.0 54 1036.0 55 1036.5 56 931.5 57 857.0 58 888.0 59 911.5 60 1000.5 61 1150.0 62 1234.0 63 1347.5 64 1495.5 65 1609.5 66 1838.0 67 1987.0 68 1997.5 69 2094.0 70 2235.0 71 2290.0 72 2284.5 73 2259.5 74 2072.0 75 1904.0 76 1809.5 77 1542.0 78 1178.0 79 987.0 80 864.0 81 594.0 82 350.0 83 253.0 84 183.0 85 84.5 86 38.5 87 21.0 88 12.5 89 2.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 68412.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.84675203180729 #Duplication Level Percentage of deduplicated Percentage of total 1 73.68475186423244 41.88738817751272 2 16.654667009514014 18.935274513243293 3 5.075854975572127 8.656376074372917 4 2.0262278220622267 4.607378822428814 5 0.933401902802777 2.6530433257323276 6 0.5554127024942145 1.8944044904402737 7 0.3317048084340447 1.3199438694965795 8 0.22370789406016972 1.0173653744957025 9 0.16456672666495242 0.8419575513067884 >10 0.30341990228850607 2.403087177688125 >50 0.020570840833119053 0.7981055955095597 >100 0.01542813062483929 1.720458399111267 >500 0.0 0.0 >1k 0.007714065312419645 5.729988890837865 >5k 0.0025713551041398816 7.535227737823774 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 5155 7.535227737823774 No Hit CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG 1513 2.2116003040402266 RNA PCR Primer, Index 34 (96% over 25bp) TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 1296 1.8944044904402737 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 1111 1.6239840963573644 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 383 0.5598433023446179 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 253 0.36981816055662753 RNA PCR Primer, Index 24 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT 174 0.25434134362392563 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT 132 0.19294860550780565 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 122 0.17833128690872946 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 113 0.1651757001695609 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 100 0.14617318599076187 No Hit CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 72 0.10524469391334855 No Hit CAGCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGC 69 0.10085949833362569 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007308659299538093 0.0 2 0.0 0.0 0.0 0.01023212301935333 0.0 3 0.0 0.0 0.0 0.011693854879260949 0.0 4 0.0 0.0 0.0 0.01900251417879904 0.0 5 0.0 0.0 0.0 0.01900251417879904 0.0 6 0.0 0.0 0.0 0.01900251417879904 0.0 7 0.0 0.0 0.0 0.02046424603870666 0.0 8 0.0 0.0 0.0 0.02192597789861428 0.0 9 0.0 0.0 0.0 0.024849441618429516 0.0 10 0.0 0.0 0.0 0.02923463719815237 0.0 11 0.0 0.0 0.0 0.03069636905805999 0.0 12 0.0 0.0 0.0 0.03069636905805999 0.0 13 0.0 0.0 0.0 0.03069636905805999 0.0 14 0.0 0.0 0.0 0.033619832777875225 0.0 15 0.0 0.0 0.0 0.033619832777875225 0.0 16 0.0 0.0 0.0 0.033619832777875225 0.0 17 0.0 0.0 0.0 0.033619832777875225 0.0 18 0.0 0.0 0.0 0.04092849207741332 0.0 19 0.0 0.0 0.0 0.04531368765713618 0.0 20 0.0 0.0 0.0 0.046775419517043795 0.0 21 0.0 0.0 0.0 0.048237151376951413 0.0 22 0.0 0.0 0.0 0.05262234695667427 0.0 23 0.0 0.0 0.0 0.05262234695667427 0.0 24 0.0 0.0 0.0 0.054084078816581886 0.0 25 0.0 0.0 0.0 0.06139273811611998 0.0 26 0.0 0.0 0.0 0.06723966555575045 0.0 27 0.0 0.0 0.0 0.12132374437233234 0.0 28 0.0 0.0 0.0 0.17248435946909899 0.0 29 0.0 0.0 0.0 0.24118575688475705 0.0 30 0.0 0.0 0.0 0.3274279366193066 0.0 31 0.0 0.0 0.0 0.41951704379348653 0.0 32 0.0 0.0014617318599076186 0.0 0.5437642518856342 0.0 33 0.0 0.0014617318599076186 0.0 0.6899374378763959 0.0 34 0.0 0.0014617318599076186 0.0 0.8901947026837397 0.0 35 0.0 0.0014617318599076186 0.0 1.1971583932643395 0.0 36 0.0 0.0014617318599076186 0.0 1.6181371689177337 0.0 37 0.0 0.0014617318599076186 0.0 2.094661755247617 0.0 38 0.0 0.0014617318599076186 0.0 2.6355025434134363 0.0 39 0.0 0.0014617318599076186 0.0 3.1398000350815645 0.0 40 0.0 0.0014617318599076186 0.0 3.69525814184646 0.0 41 0.0 0.0014617318599076186 0.0 4.2828743495293224 0.0 42 0.0 0.0014617318599076186 0.0 4.988890837864702 0.0 43 0.0 0.0014617318599076186 0.0 5.67736654388119 0.0 44 0.0 0.0014617318599076186 0.0 6.345378003858972 0.0 45 0.0 0.0014617318599076186 0.0 6.957843653160264 0.0 46 0.0 0.0014617318599076186 0.0 7.624393381278138 0.0 47 0.0 0.0014617318599076186 0.0 8.238320762439338 0.0 48 0.0 0.0014617318599076186 0.0 8.766005963865988 0.0 49 0.0 0.0014617318599076186 0.0 9.300999824592177 0.0 50 0.0 0.0014617318599076186 0.0 9.81406770741975 0.0 51 0.0 0.0014617318599076186 0.0 10.311056539788341 0.0 52 0.0 0.0014617318599076186 0.0 10.936677775828802 0.0 53 0.0 0.0014617318599076186 0.0 11.626615213705199 0.0 54 0.0 0.0014617318599076186 0.0 12.243466058586213 0.0 55 0.0 0.0014617318599076186 0.0 12.798924165351108 0.0 56 0.0 0.0014617318599076186 0.0 13.323685903057942 0.0 57 0.0 0.0014617318599076186 0.0 13.830906858445887 0.0 58 0.0 0.0014617318599076186 0.0 14.30158451733614 0.0 59 0.0 0.0014617318599076186 0.0 14.709407706250365 0.0 60 0.0 0.0014617318599076186 0.0 15.152312459802374 0.0 61 0.0 0.0014617318599076186 0.0 15.628837046132258 0.0 62 0.0 0.0014617318599076186 0.0 16.125825878500848 0.0 63 0.0 0.0014617318599076186 0.0 16.64035549318833 0.0 64 0.0 0.0014617318599076186 0.0 17.188504940653687 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTCTT 15 0.0022159447 70.00001 27 CTTTATA 65 0.0 64.61539 2 TCTTTAT 60 0.0 64.16667 1 TTAATAC 20 0.006924257 52.5 3 ATTCGAG 20 0.006924257 52.5 48 AAGCGAG 20 0.006924257 52.5 15 AGTGCTA 20 0.006924257 52.5 53 GAATTAT 20 0.006924257 52.5 43 AGTAAGG 20 0.006924257 52.5 2 TGCGTCG 20 0.006924257 52.5 45 GAGAAGA 30 5.8404944E-4 46.666668 38 TTTATAC 90 0.0 46.666664 3 TAGCACC 55 1.2885175E-7 44.545452 33 AGCACCT 60 2.5522786E-7 40.833336 34 ACAACAA 35 0.0012475083 40.0 70 GTGTGGG 35 0.0012475083 40.0 47 GTATAGG 35 0.0012475083 40.0 1 TGCTATA 35 0.0012475083 40.0 55 GCACCTC 65 4.781359E-7 37.69231 35 CTCGTAT 1270 0.0 35.0 39 >>END_MODULE