FastQCFastQC Report
Wed 25 May 2016
SRR1780191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309778
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT353.192278E-431.46220414
ACAGAGT300.00571308229.35997436
GACGATT300.0057447829.32676323
AAACTCG300.005753861729.3172927
GAAACTC300.005753861729.3172926
AGTCTGT300.00576295529.30781742
AAAGGCC554.8736038E-628.05266218
TGTATAA554.9602677E-627.98920211
TCAGATG554.9978153E-627.9620974
CTTGTTT406.996285E-427.51607312
GTTAGCT450.00140560624.4152957
GAATATG450.00140560624.4152951
CAATGTG952.6937414E-823.1377532
CGAGATA500.002584340721.98796525
GTTCATG500.002594055621.9737661
TAGCTTG500.002594055621.9737669
TTACTCC500.00259648921.9702174
TATTCTA500.00259648921.9702175
CGGAGTG755.492475E-520.51547232
ACGAAAC550.004469448220.00521924
Produced by FastQC (version 0.11.5)