FastQCFastQC Report
Wed 25 May 2016
SRR1780191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309778
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT168535.44034760376786No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG44591.4394179057260361No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC42021.356455267966092No Hit
CACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCTG35491.1456591494554165TruSeq Adapter, Index 2 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC11190.36122642666683885No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT5700.18400273744423426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT207.8424625E-444.0058144
ACAGAAG207.8424625E-444.0058143
CTCTAGC207.8424625E-444.0058142
TCTTCTG5400.037.4803844
TGGGATT301.2991257E-436.6655922
CTTGTAC250.00234857835.204653
CCCTATG250.002350439335.19896713
TGCATAA250.002350439335.19896714
CTGCATA250.002350439335.19896713
ACTTTGC250.002350439335.19896738
TGTACTA250.002350439335.1989675
TCTTTAT1400.034.598341
GTCTTCT5850.034.59727543
CGTCTTC5900.034.30407742
GCTTATA451.0328422E-634.226743
CTTAATA803.6379788E-1233.004362
TTAATAC803.6379788E-1233.004363
CCGTCTT6300.032.1260441
TGGACTG353.2129342E-431.427655
TCCCTAT353.2129342E-431.4276512