FastQCFastQC Report
Wed 25 May 2016
SRR1780190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87270
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG150.002218001370.063
ATCCTTC200.00693063252.559
AAGTAAT200.00693063252.557
GAAGCGA200.00693063252.542
TTATGCC200.00693063252.546
ACTTCGC200.00693063252.565
GCTCTAC200.00693063252.51
TGGTCTA200.00693063252.543
ATCTCAT305.849418E-446.66666423
CGACGAA350.001249402940.022
CTTTGAG350.001249402940.032
CTGCAGC556.6283537E-638.181821
CATTAAG400.002407241835.053
CCTTTGA400.002407241835.031
AAAAAGA450.00428688531.11110951
CGCCAAC450.00428688531.11110921
TAGATCT703.4543933E-530.039
TTGGCTT500.00717447827.9999984
GACAACA500.00717447827.99999840
GTTAGGA500.00717447827.9999983