##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780190_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87270 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.109476337802224 34.0 33.0 34.0 21.0 34.0 2 31.240311676406556 34.0 34.0 34.0 21.0 34.0 3 31.18657041365876 34.0 34.0 34.0 23.0 34.0 4 31.037492838317863 34.0 33.0 34.0 21.0 34.0 5 31.133952102669873 34.0 33.0 34.0 21.0 34.0 6 34.30863985332875 38.0 36.0 38.0 23.0 38.0 7 34.53859287269394 38.0 36.0 38.0 24.0 38.0 8 34.597616592185176 38.0 36.0 38.0 24.0 38.0 9 34.78833505213704 38.0 37.0 38.0 25.0 38.0 10-11 34.5762805087659 38.0 36.0 38.0 23.5 38.0 12-13 34.61133264581185 38.0 36.0 38.0 24.0 38.0 14-15 34.55503036553226 38.0 36.0 38.0 23.5 38.0 16-17 34.592752377678465 38.0 36.0 38.0 23.5 38.0 18-19 34.608519537068865 38.0 36.0 38.0 23.5 38.0 20-21 34.61534891715366 38.0 36.0 38.0 23.5 38.0 22-23 34.601334937550135 38.0 36.0 38.0 23.5 38.0 24-25 34.63776211756617 38.0 36.0 38.0 23.5 38.0 26-27 34.648647874412745 38.0 36.0 38.0 24.0 38.0 28-29 34.73119628738398 38.0 37.0 38.0 24.0 38.0 30-31 34.762724876819064 38.0 37.0 38.0 25.0 38.0 32-33 34.80398762461327 38.0 37.0 38.0 25.0 38.0 34-35 34.77975249226539 38.0 37.0 38.0 25.0 38.0 36-37 34.7027558152859 38.0 37.0 38.0 24.5 38.0 38-39 34.70560902944884 38.0 36.5 38.0 24.0 38.0 40-41 34.76082846338948 38.0 37.0 38.0 24.5 38.0 42-43 34.803088117337 38.0 37.0 38.0 25.0 38.0 44-45 34.87598258278905 38.0 37.0 38.0 26.0 38.0 46-47 34.790798670791794 38.0 37.0 38.0 25.0 38.0 48-49 34.79463160307093 38.0 37.0 38.0 25.0 38.0 50-51 34.72281425461212 38.0 37.0 38.0 24.5 38.0 52-53 34.77778159734159 38.0 37.0 38.0 25.0 38.0 54-55 34.75779763950957 38.0 37.0 38.0 25.0 38.0 56-57 34.71529162369657 38.0 37.0 38.0 24.5 38.0 58-59 34.758382032771856 38.0 37.0 38.0 24.5 38.0 60-61 34.78335052137046 38.0 37.0 38.0 25.0 38.0 62-63 34.659527901913606 38.0 37.0 38.0 24.0 38.0 64-65 34.624235132347884 38.0 36.0 38.0 24.0 38.0 66-67 34.559831557236166 38.0 36.0 38.0 24.0 38.0 68-69 34.535040678354534 38.0 36.0 38.0 24.0 38.0 70-71 34.48182078606624 38.0 36.0 38.0 23.5 38.0 72-73 34.531883808869026 38.0 36.0 38.0 23.5 38.0 74-75 34.601587028761315 38.0 36.0 38.0 24.0 38.0 76 33.08430159275811 37.0 33.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07206366216065518 1101 2 3.746261245112237E-4 1101 3 0.3317570947662176 1101 4 -0.15389610027261824 1101 5 -0.20723435771312992 1101 6 -0.1565043912969415 1101 7 -0.2431951463988895 1101 8 -0.47868556139905394 1101 9 -0.2450404532348216 1101 10-11 -0.26100936622992776 1101 12-13 0.15189781225009114 1101 14-15 -0.2202107906614259 1101 16-17 0.11751823843649589 1101 18-19 -0.21852282094158682 1101 20-21 -0.25311083484467645 1101 22-23 -0.023588749557575284 1101 24-25 -0.1333237926213826 1101 26-27 -0.34256535222502293 1101 28-29 -0.2591502919000064 1101 30-31 -0.4791233415175711 1101 32-33 -0.4047749278416859 1101 34-35 -0.3008366901314403 1101 36-37 -0.2594605484437338 1101 38-39 -0.11831073819009674 1101 40-41 -0.03318774434364968 1101 42-43 -0.5636887264511472 1101 44-45 -0.34395967751320455 1101 46-47 -0.05329607171314166 1101 48-49 -0.08051869669169065 1101 50-51 -0.062406445371713914 1101 52-53 -0.36216988002915684 1101 54-55 -0.38870724689226677 1101 56-57 -0.11573125897462688 1101 58-59 -0.008037401778999254 1101 60-61 0.347911587150044 1101 62-63 -0.3074457792057501 1101 64-65 -0.04737880339785505 1101 66-67 -0.20222178988527162 1101 68-69 -0.13455896091775799 1101 70-71 -0.13673811650692613 1101 72-73 -0.5202400028263767 1101 74-75 -0.5255722857353717 1101 76 -2.001028166264632 1102 1 -0.9968023381188438 1102 2 -0.717308300704758 1102 3 -0.5989049261397064 1102 4 -0.5081643929555462 1102 5 -0.6388545667723626 1102 6 -0.6742744261401441 1102 7 -0.4783867840295457 1102 8 -0.9724137843955702 1102 9 -0.4656850525379568 1102 10-11 -0.9174553592612824 1102 12-13 -0.6084244874014857 1102 14-15 -1.0938606164454043 1102 16-17 -0.5962883817725597 1102 18-19 -0.38328762930395754 1102 20-21 -0.8719697198620935 1102 22-23 -0.5958884011255208 1102 24-25 -0.928271527813024 1102 26-27 -1.1054573382877422 1102 28-29 -0.8485666682066295 1102 30-31 -0.7862226446534564 1102 32-33 -0.5650536734862897 1102 34-35 -0.46176874588056904 1102 36-37 -0.446394346353145 1102 38-39 -1.0185285082249393 1102 40-41 -1.0541807757025339 1102 42-43 -0.7576347194825033 1102 44-45 -0.611424834307627 1102 46-47 -0.9025556535946748 1102 48-49 -0.9609193935558054 1102 50-51 -0.8226416370093474 1102 52-53 -0.7648702284612128 1102 54-55 -0.9344825082163055 1102 56-57 -0.5749211544450006 1102 58-59 -0.5677063913260412 1102 60-61 -0.5202939877628481 1102 62-63 -0.7230381137005253 1102 64-65 -0.6667167824919389 1102 66-67 -0.5862374693277843 1102 68-69 -0.8167453720675866 1102 70-71 -0.5560760956010071 1102 72-73 -0.46749610038735057 1102 74-75 -0.9304503345158537 1102 76 -0.15329297462699998 1103 1 0.8262729327512837 1103 2 0.5709714950090508 1103 3 0.5748099323396048 1103 4 0.6802037040326638 1103 5 0.5914447029522307 1103 6 0.08067760478916597 1103 7 0.8071962430336015 1103 8 0.4873614053328481 1103 9 0.5788832258258481 1103 10-11 0.27162272770353724 1103 12-13 0.912881178199207 1103 14-15 0.7380328492602928 1103 16-17 0.6026699018415869 1103 18-19 0.8421670029331665 1103 20-21 0.664770769851998 1103 22-23 0.636391680970803 1103 24-25 0.7190245439735321 1103 26-27 0.8331195792818207 1103 28-29 0.6371559703309089 1103 30-31 0.4672318443924155 1103 32-33 0.3875440545457849 1103 34-35 0.13984334900749928 1103 36-37 0.7169093145699676 1103 38-39 0.30813251034219746 1103 40-41 0.6689648975350195 1103 42-43 0.3419130348013013 1103 44-45 0.5471117510582246 1103 46-47 0.5221197795588708 1103 48-49 0.5248629080049838 1103 50-51 0.03213856441302454 1103 52-53 0.4952665191880641 1103 54-55 0.7846538098592006 1103 56-57 0.7191757860351586 1103 58-59 0.4144185669098377 1103 60-61 0.4968352662107094 1103 62-63 0.40229003292732557 1103 64-65 1.0338345038428471 1103 66-67 0.49638388526149413 1103 68-69 0.9060721545421231 1103 70-71 0.6598861496378916 1103 72-73 0.8526318171344869 1103 74-75 0.6317662661237335 1103 76 0.8544512857901623 1104 1 0.026939660098481255 1104 2 -0.16881142038711872 1104 3 0.1214580914439587 1104 4 0.4440274877672472 1104 5 -0.651171234789544 1104 6 -0.5777668497687003 1104 7 -0.2481785351032073 1104 8 -0.4624064916316115 1104 9 -0.24163513762020017 1104 10-11 -0.1852618579242744 1104 12-13 -0.22729653990938203 1104 14-15 0.32393372761099926 1104 16-17 0.2036062783036101 1104 18-19 -0.24796634253627303 1104 20-21 -0.43695634979484055 1104 22-23 -0.0792365900891383 1104 24-25 0.11102836684705153 1104 26-27 0.11640474744275053 1104 28-29 0.21597429281759872 1104 30-31 0.38993812027645447 1104 32-33 0.291238361194857 1104 34-35 0.33350716368915556 1104 36-37 0.018114202386833256 1104 38-39 0.12392348107901086 1104 40-41 0.21074913273276508 1104 42-43 0.33668502770167663 1104 44-45 0.3743542759751648 1104 46-47 0.2848683801140979 1104 48-49 0.09713911061728453 1104 50-51 0.3128011958242993 1104 52-53 0.07894307678147072 1104 54-55 0.20128444745989782 1104 56-57 -0.005639896848371961 1104 58-59 -0.02423341506803922 1104 60-61 0.13022055724970727 1104 62-63 0.3941322938839491 1104 64-65 0.09464777467523078 1104 66-67 0.20928153237386482 1104 68-69 0.2512799095141318 1104 70-71 -0.09039260155676487 1104 72-73 0.025980927406180854 1104 74-75 0.05715196675632228 1104 76 0.4936562191174261 1105 1 0.3707934806964879 1105 2 0.29876868173333193 1105 3 0.4406736815351522 1105 4 0.3891767664579646 1105 5 0.5320992952111254 1105 6 0.7290181398631717 1105 7 0.6781854863911434 1105 8 0.7650344769518611 1105 9 0.7444849542436103 1105 10-11 0.6904767315648996 1105 12-13 -0.4577977773951645 1105 14-15 0.4205969753980199 1105 16-17 0.8339492067969942 1105 18-19 0.6282293362971458 1105 20-21 0.46260105393768924 1105 22-23 0.4984630241720467 1105 24-25 0.43153718532684593 1105 26-27 0.6581057887145718 1105 28-29 0.6387763619830196 1105 30-31 0.3866604552226036 1105 32-33 0.42000934828486436 1105 34-35 0.8200286022942578 1105 36-37 0.3895327401468691 1105 38-39 0.8859917546191056 1105 40-41 0.833566809827019 1105 42-43 0.6113232582084862 1105 44-45 1.0353667846152774 1105 46-47 0.2236574278074741 1105 48-49 0.5752099706141607 1105 50-51 0.530414743506519 1105 52-53 0.35027019667266757 1105 54-55 0.4231455814874039 1105 56-57 0.5812126528182873 1105 58-59 0.7135589548844692 1105 60-61 0.7773698805345077 1105 62-63 0.4689444413119901 1105 64-65 -0.0941189759769614 1105 66-67 0.73817849774656 1105 68-69 0.622308249053475 1105 70-71 0.5551123149408212 1105 72-73 0.48166555805569544 1105 74-75 0.5462877334400815 1105 76 -0.020491253512957996 1106 1 -0.2515841416811355 1106 2 0.025924076673959462 1106 3 0.501287564296689 1106 4 0.08134365996714266 1106 5 -0.2488177742965476 1106 6 -0.07738351217606265 1106 7 -0.1912470944508371 1106 8 0.023212540499052636 1106 9 -0.0831773163716818 1106 10-11 -0.3920782972988519 1106 12-13 -0.2690687212164491 1106 14-15 -0.5361073940580283 1106 16-17 -0.37257916416090353 1106 18-19 -0.2758280157467823 1106 20-21 -0.2889125331463731 1106 22-23 -0.2845777605465969 1106 24-25 -0.45170541100300454 1106 26-27 -0.3341737438334178 1106 28-29 0.014838219588504842 1106 30-31 0.07740762751340213 1106 32-33 -0.05187782494458304 1106 34-35 0.023776196981444286 1106 36-37 -0.15351649249968347 1106 38-39 -0.18383271621207342 1106 40-41 -0.12142450758041434 1106 42-43 -0.34264726790969036 1106 44-45 -0.5333078293613553 1106 46-47 -0.18787399379106517 1106 48-49 -0.3038203949933873 1106 50-51 -0.6076736781389442 1106 52-53 -0.24176528462456304 1106 54-55 -0.4907676864527062 1106 56-57 -0.29791157865494 1106 58-59 -0.4007696695112699 1106 60-61 -0.3601553459168869 1106 62-63 -0.32450372126369587 1106 64-65 0.04827554225648356 1106 66-67 -0.467094417257897 1106 68-69 -0.7982577621165632 1106 70-71 -0.45472013448894444 1106 72-73 -0.45314459823097053 1106 74-75 -0.5256721858352691 1106 76 -0.14997921521568003 1107 1 -0.3284947755312686 1107 2 -0.7194906509471721 1107 3 -0.9977294329409503 1107 4 -1.004264681868957 1107 5 -0.8868861116531406 1107 6 -0.6875669230712163 1107 7 -0.6893059750719956 1107 8 -1.4048167256085051 1107 9 -0.2330043576577978 1107 10-11 -0.7357933011562068 1107 12-13 -0.6232483494174303 1107 14-15 -0.6895740341794792 1107 16-17 -1.0557723055391008 1107 18-19 -0.5153136191113958 1107 20-21 -0.3843322379966807 1107 22-23 -0.7268170159531024 1107 24-25 -0.7119460600336822 1107 26-27 -0.9630279856769803 1107 28-29 -0.4638592395359993 1107 30-31 -0.3040534381113673 1107 32-33 -0.22124671228500858 1107 34-35 -0.5553804115345926 1107 36-37 -0.7930904416821747 1107 38-39 -0.677468084402598 1107 40-41 -0.5680250600528467 1107 42-43 -0.4209141967104273 1107 44-45 -0.0721053307923114 1107 46-47 -0.4047132044025119 1107 48-49 -0.39123298240597393 1107 50-51 -0.21984798070470646 1107 52-53 -0.3746635251740429 1107 54-55 -0.3084517817168688 1107 56-57 -0.27652815780532336 1107 58-59 -0.27958036059953884 1107 60-61 -0.042365484532723485 1107 62-63 -0.13447933284072633 1107 64-65 -0.04598073527380819 1107 66-67 -0.2074137065095698 1107 68-69 -0.1380096472421144 1107 70-71 -0.07946816226188247 1107 72-73 0.1379386956992974 1107 74-75 0.2585151570709314 1107 76 -0.1686438246276225 1108 1 -0.46377901000983357 1108 2 -0.5531436957023637 1108 3 -0.06436611288115301 1108 4 -0.11118500096300821 1108 5 0.052258472876353323 1108 6 0.5554446262282866 1108 7 0.4108144128151352 1108 8 0.2189777418404617 1108 9 -0.11940317230013875 1108 10-11 -0.08793448572009765 1108 12-13 0.2782055658347886 1108 14-15 0.2270077435924236 1108 16-17 0.3001988013679906 1108 18-19 0.65092088590918 1108 20-21 -0.17651099416490723 1108 22-23 0.058859471366474736 1108 24-25 0.24407928756980368 1108 26-27 0.569516432161592 1108 28-29 0.25852761569425553 1108 30-31 0.04451579815317075 1108 32-33 0.3227874725831654 1108 34-35 0.24115082978359226 1108 36-37 -0.12581742576715982 1108 38-39 0.36070545936699716 1108 40-41 0.3057793294747313 1108 42-43 0.03364929797795213 1108 44-45 -0.07928230098637812 1108 46-47 0.568378118409008 1108 48-49 0.05486367186912133 1108 50-51 0.10916153126131434 1108 52-53 0.23863999782533085 1108 54-55 0.040401887467794495 1108 56-57 0.04234760454104247 1108 58-59 0.08244321425923573 1108 60-61 0.4272663402036798 1108 62-63 0.3537464672095467 1108 64-65 0.22837796771207053 1108 66-67 0.32212101415083794 1108 68-69 0.4143855425341769 1108 70-71 0.19961345014203857 1108 72-73 -0.053438356517297336 1108 74-75 0.18032256291040483 1108 76 0.15919488206250065 1109 1 -0.5042065193035121 1109 2 -0.32473698101834714 1109 3 -0.1523946909480678 1109 4 -0.11137377884404742 1109 5 0.3184352851440124 1109 6 -0.44366956986836925 1109 7 -0.5065880035417436 1109 8 -1.0262302042561942 1109 9 -0.3043038460919618 1109 10-11 -0.3667013305156388 1109 12-13 -0.2579236163213423 1109 14-15 -0.4296415942328551 1109 16-17 -0.3095464937063568 1109 18-19 -0.10892460665587578 1109 20-21 -0.5970282455589597 1109 22-23 0.2266902682995635 1109 24-25 -0.49766531047852425 1109 26-27 -0.5050653522250244 1109 28-29 -0.8058020776142953 1109 30-31 -0.036433609374711295 1109 32-33 -0.4466276064131165 1109 34-35 -0.6010599044171556 1109 36-37 -0.2836792984437366 1109 38-39 -0.37238439890438 1109 40-41 -0.2716922086293607 1109 42-43 -0.6237333693082903 1109 44-45 -0.2810132489417754 1109 46-47 -0.25463535742742494 1109 48-49 -0.24865485740597393 1109 50-51 -0.7273059989431445 1109 52-53 -0.28499354074344296 1109 54-55 -0.1943434076065529 1109 56-57 -0.09848795540319344 1109 58-59 -0.5088744553504299 1109 60-61 -0.11486743070710048 1109 62-63 -0.44801497563432235 1109 64-65 -0.20139666054071625 1109 66-67 -0.5537284863138439 1109 68-69 -0.19599869306061635 1109 70-71 -0.055599723649784494 1109 72-73 -0.1970257171120906 1109 74-75 -0.21262585716394256 1109 76 -0.09321566626463351 1110 1 -0.813617165691344 1110 2 -0.17910663406017235 1110 3 -0.2235389399974963 1110 4 -0.5561367303649583 1110 5 -0.6418486977457221 1110 6 -0.5447716373588634 1110 7 -0.33037602635543095 1110 8 -0.1435959361953607 1110 9 -0.10624360369652663 1110 10-11 -0.23423044173236462 1110 12-13 -0.5862268482605018 1110 14-15 -0.31660541314920465 1110 16-17 -0.6246531358166223 1110 18-19 -0.593923418442948 1110 20-21 -0.11681941713690946 1110 22-23 -0.3778798956738143 1110 24-25 -0.3516562206496303 1110 26-27 -0.29332580849878553 1110 28-29 0.0598407455796206 1110 30-31 -0.47839683418459344 1110 32-33 -0.2763121358808007 1110 34-35 -0.47401023711677226 1110 36-37 -0.2550879248695921 1110 38-39 -0.6502702710526691 1110 40-41 -0.16848106319209677 1110 42-43 -0.3147683027683641 1110 44-45 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-0.026978494370119677 2116 14-15 0.19969339052324386 2116 16-17 0.15153391787900716 2116 18-19 0.4533848445636366 2116 20-21 0.48038219651421343 2116 22-23 0.6406098567141925 2116 24-25 0.5543068693995252 2116 26-27 0.1641419648481488 2116 28-29 0.620074446775952 2116 30-31 0.017348783917967125 2116 32-33 -0.04066342958384439 2116 34-35 0.5046946687535723 2116 36-37 0.08017359789772627 2116 38-39 0.1853042443882984 2116 40-41 -0.1060100439952123 2116 42-43 -0.11782635711316658 2116 44-45 0.31235565349724936 2116 46-47 0.49117256940184717 2116 48-49 0.38315726149646423 2116 50-51 0.42518066264222654 2116 52-53 0.33443290742728493 2116 54-55 0.42222480885686764 2116 56-57 0.18814504764558393 2116 58-59 0.4200984867227433 2116 60-61 0.2074934686831469 2116 62-63 0.22600369814616528 2116 64-65 0.3180393150690364 2116 66-67 0.5095813460032304 2116 68-69 0.2951884258418289 2116 70-71 0.321972684886795 2116 72-73 0.44931574630254545 2116 74-75 0.42495036234825534 2116 76 0.7404352483695149 2117 1 0.3639441656279949 2117 2 0.040149577200832454 2117 3 0.20494691864097447 2117 4 0.7212995944436251 2117 5 0.13254059336319202 2117 6 0.39574609793984905 2117 7 -0.05239279806229291 2117 8 0.20311482999038333 2117 9 0.26723939020941145 2117 10-11 0.3247049194843612 2117 12-13 -0.03061197248572256 2117 14-15 0.8921424383013914 2117 16-17 -0.3100013310351244 2117 18-19 0.6092902906043989 2117 20-21 0.12709953697528675 2117 22-23 0.2802272974091551 2117 24-25 0.5614902973981941 2117 26-27 0.6190784833914122 2117 28-29 -0.23441937213484465 2117 30-31 0.25319074850200707 2117 32-33 0.033091999751263756 2117 34-35 0.5216926640759922 2117 36-37 0.5884846570357141 2117 38-39 0.6968311404791834 2117 40-41 0.33848544548023085 2117 42-43 0.600474678636921 2117 44-45 0.5094405181815134 2117 46-47 0.3936951220246314 2117 48-49 0.31424646964882186 2117 50-51 0.6040563726321935 2117 52-53 0.7914993959003951 2117 54-55 0.6254072345167287 2117 56-57 0.6078744161721445 2117 58-59 0.7260624025262814 2117 60-61 0.3273147182655052 2117 62-63 0.1762626356670438 2117 64-65 0.45506738055517104 2117 66-67 0.5991236112888956 2117 68-69 0.11840092166541183 2117 70-71 1.0759820400488493 2117 72-73 0.2670153787783178 2117 74-75 0.46053338941139543 2117 76 1.0154101898997467 2118 1 0.5240192871481 2118 2 0.9440382205945568 2118 3 0.94765571227774 2118 4 0.5538227937365967 2118 5 0.5203002044988523 2118 6 1.1068596640025987 2118 7 0.7061136093614806 2118 8 1.0042637473567098 2118 9 0.7500056297144866 2118 10-11 0.4174468549682331 2118 12-13 0.8156650933560812 2118 14-15 0.9814597162510168 2118 16-17 1.1285205425839635 2118 18-19 0.8147145062473555 2118 20-21 0.7486748794410403 2118 22-23 0.28459097394472366 2118 24-25 0.8625287419408352 2118 26-27 0.45259593809755927 2118 28-29 1.4031192933534484 2118 30-31 0.7840679027220645 2118 32-33 1.0093955790707554 2118 34-35 0.6643130794538123 2118 36-37 0.8792491289756228 2118 38-39 0.4923459438375559 2118 40-41 0.7783329929835432 2118 42-43 0.6446638081110692 2118 44-45 0.518885944606609 2118 46-47 0.5533283522499985 2118 48-49 0.4353799208198339 2118 50-51 0.8053930937987914 2118 52-53 0.5263093826436531 2118 54-55 0.6590462093289347 2118 56-57 0.5592111373387425 2118 58-59 0.26903633093989043 2118 60-61 0.2284415410670917 2118 62-63 0.243706294526973 2118 64-65 0.3172525330076681 2118 66-67 0.8670754451377718 2118 68-69 0.07321753677808829 2118 70-71 0.3754738650598952 2118 72-73 1.1134581538556532 2118 74-75 0.1941281750941215 2118 76 0.39090731760633446 2119 1 0.5345102948557816 2119 2 0.5146919459728032 2119 3 0.223918915246621 2119 4 1.3792581348727637 2119 5 1.3592953515156907 2119 6 1.3317383394236657 2119 7 1.5855027044228578 2119 8 1.0926165877792045 2119 9 0.9424121384213109 2119 10-11 1.4928465124047108 2119 12-13 0.8623750562450283 2119 14-15 0.9418151258998861 2119 16-17 1.015795735275944 2119 18-19 1.1222009464414668 2119 20-21 0.7453562953702431 2119 22-23 1.121480782680095 2119 24-25 1.0614296839905037 2119 26-27 1.0507974796333812 2119 28-29 0.7840071038016347 2119 30-31 1.2022451793420998 2119 32-33 0.6887775373921983 2119 34-35 0.9024660911580682 2119 36-37 0.9365571506713124 2119 38-39 1.0018378079540255 2119 40-41 0.38549114314055544 2119 42-43 1.0736532457359615 2119 44-45 0.883394936898938 2119 46-47 0.6099940673513373 2119 48-49 0.8609794069745575 2119 50-51 0.802716816322345 2119 52-53 1.268708754610543 2119 54-55 1.1409545735881395 2119 56-57 1.0675968067649464 2119 58-59 1.1795416121274442 2119 60-61 1.1313818779212212 2119 62-63 1.265152198038244 2119 64-65 0.8764517907867173 2119 66-67 0.7238053908985478 2119 68-69 0.7851787129128383 2119 70-71 0.9561190263502155 2119 72-73 1.166912335985252 2119 74-75 0.5729106472608407 2119 76 1.6688833381601427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 11.0 13 144.0 14 584.0 15 924.0 16 1229.0 17 1388.0 18 1348.0 19 1123.0 20 837.0 21 646.0 22 474.0 23 431.0 24 366.0 25 387.0 26 410.0 27 559.0 28 604.0 29 752.0 30 995.0 31 1274.0 32 1666.0 33 2274.0 34 3422.0 35 5694.0 36 12095.0 37 47633.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.9122264237424 20.58553913143119 14.211069095909249 27.29116534891715 2 20.207402314655667 19.515297353042282 34.43565944769107 25.841640884610978 3 18.7658989343417 21.583591153890225 27.633780222298615 32.016729689469464 4 15.390168442763835 19.145181620258967 31.424315343187807 34.04033459378939 5 15.82789045491005 33.720637103242815 31.60192506015813 18.84954738168901 6 30.872006416867194 32.6034146900424 17.00698980176464 19.51758909132577 7 28.70745960811275 26.02612581643176 21.79672281425461 23.46969176120087 8 24.77369084450556 32.44643061762347 19.244872235590694 23.53500630228028 9 23.9234559413315 17.160536266758335 20.30021771513693 38.61579007677323 10-11 27.023031969749056 23.43187807952332 24.4024292425805 25.14266070814713 12-13 25.409648218173487 21.06508536725106 24.020854818379743 29.504411596195713 14-15 24.701069674972356 22.158371481445407 21.824348712895112 31.31621013068712 16-17 25.48871318895382 23.808296092586225 22.928268591726823 27.774722126733124 18-19 24.33253122493411 23.231350979718115 24.17153661051908 28.264581184828693 20-21 24.384668270883463 23.73496046751461 23.999083304686604 27.88128795691532 22-23 25.925862266529165 23.191818494327947 23.259997708261718 27.622321530881173 24-25 24.930101982353612 24.039761659218517 23.06634582330698 27.963790535120893 26-27 24.162369657385128 24.156067377105533 24.201902142775296 27.479660822734044 28-29 24.928383178641 23.37515755700699 24.920935029219663 26.775524235132348 30-31 24.83384897444712 23.19468316718231 24.496963446774377 27.474504411596197 32-33 24.295290477827432 23.71548069210496 24.64535350063023 27.343875329437378 34-35 24.51128681104618 23.727512318093275 24.73988770482411 27.021313166036435 36-37 25.047553569382373 23.131087429815516 24.37722012146213 27.44413887933998 38-39 23.956113211871205 23.10415950498453 26.09659676864902 26.843130514495243 40-41 24.12913945227455 22.938008479431648 25.74653374584622 27.186318322447576 42-43 23.274321072533517 22.99243726366449 25.776899278102437 27.95634238569955 44-45 22.72774149192162 22.927122722585082 26.55895496734273 27.78618081815057 46-47 23.101294832130172 23.1293686261029 26.49421336083419 27.275123180932738 48-49 22.980405637676178 23.23249684885986 26.38191818494328 27.405179328520685 50-51 22.897903059470607 23.804858485160995 25.393606050189067 27.90363240517933 52-53 22.30491577861808 23.661624842442993 25.831900996906153 28.201558382032772 54-55 22.166265612467058 23.691417440128337 25.660020625644552 28.482296321760053 56-57 22.302624040334592 23.878767044803485 25.475535693823765 28.343073221038157 58-59 22.65784347427524 23.596310301363584 25.351208891944538 28.39463733241664 60-61 22.543256560100836 23.095565486421453 25.317978686833964 29.04319926664375 62-63 22.265956227798785 23.314426492494558 25.518505786639167 28.901111493067493 64-65 23.05202245903518 23.302967801077116 24.938123066345824 28.70688667354188 66-67 22.811962873839807 23.721782972384553 24.072991864329094 29.393262289446547 68-69 22.622321530881173 24.174401283373438 24.20304801191704 29.00022917382835 70-71 23.653603758450785 23.776784691188265 24.10393033115618 28.465681219204768 72-73 23.390626790420534 23.73209579466025 23.704022000687523 29.173255414231697 74-75 22.803368855276727 24.351438065772886 23.84095336312593 29.004239715824454 76 22.76956571559528 23.78938925174745 23.396356136129253 30.044688896528015 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 2.0 15 0.0 16 3.0 17 6.0 18 5.5 19 9.5 20 15.5 21 17.0 22 24.5 23 37.0 24 62.0 25 82.0 26 139.5 27 237.5 28 343.0 29 408.0 30 477.5 31 629.0 32 895.0 33 1079.0 34 1242.0 35 1614.0 36 1937.0 37 2051.0 38 2307.0 39 2979.0 40 3638.0 41 4078.5 42 4276.0 43 4316.5 44 4614.0 45 4747.5 46 4624.0 47 4712.5 48 4558.5 49 3989.5 50 3663.0 51 3466.5 52 2961.0 53 2411.5 54 2171.0 55 2017.0 56 1732.5 57 1478.5 58 1355.0 59 1319.5 60 1295.0 61 1336.0 62 1366.0 63 1425.0 64 1539.0 65 1620.0 66 1727.5 67 1809.0 68 1844.5 69 1921.5 70 1966.0 71 1969.0 72 1969.5 73 1850.5 74 1594.5 75 1458.0 76 1346.0 77 1066.0 78 745.5 79 593.0 80 480.5 81 272.0 82 122.5 83 69.0 84 58.5 85 35.0 86 15.0 87 8.0 88 5.5 89 3.0 90 1.5 91 1.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 1.0 98 2.5 99 10.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.729345708720064E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 87270.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.85814140025209 #Duplication Level Percentage of deduplicated Percentage of total 1 77.18243643345475 49.287269393835224 2 12.083475389832941 15.432565601008367 3 4.148647921190045 7.947748367136473 4 2.1855766297618833 5.582674458576831 5 1.2883776848678425 4.113670218861007 6 0.8505445997595507 3.258851839119972 7 0.5903569057402789 2.6389366334364617 8 0.4503938703368085 2.3009052366219778 9 0.34631879272909977 1.9903746992093505 >10 0.8738717723267958 7.44700355219434 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001145869141744013 0.0 2 0.0 0.0 0.0 0.0034376074252320385 0.0 3 0.0 0.0 0.0 0.0034376074252320385 0.0 4 0.0 0.0 0.0 0.011458691417440128 0.0 5 0.0 0.0 0.0 0.014896298842672167 0.0 6 0.0 0.0 0.0 0.014896298842672167 0.0 7 0.0 0.0 0.0 0.01604216798441618 0.0 8 0.0 0.0 0.0 0.01604216798441618 0.0 9 0.0 0.0 0.0 0.021771513693136242 0.0 10 0.0 0.0 0.0 0.022917382834880255 0.0 11 0.0 0.0 0.0 0.022917382834880255 0.0 12 0.0 0.0 0.0 0.022917382834880255 0.0 13 0.0 0.0 0.0 0.022917382834880255 0.0 14 0.0 0.0 0.0 0.026354990260112295 0.0 15 0.0 0.0 0.0 0.026354990260112295 0.0 16 0.0 0.0 0.0 0.02864672854360032 0.0 17 0.0 0.0 0.0 0.02864672854360032 0.0 18 0.0 0.0 0.0 0.030938466827088347 0.0 19 0.0 0.0 0.0 0.03323020511057637 0.0 20 0.0 0.0 0.0 0.037813681677552426 0.0 21 0.0 0.0 0.0 0.03895955081929644 0.0 22 0.0 0.0 0.0 0.04239715824452848 0.0 23 0.0 0.0 0.0 0.04583476566976051 0.0 24 0.0 0.0 0.0 0.04927237309499255 0.0 25 0.0 0.0 0.0 0.051564111378480576 0.0 26 0.0 0.0 0.0 0.06073106451243268 0.0 27 0.0 0.0 0.0 0.10083648447347313 0.0 28 0.0 0.0 0.0 0.14552538100148962 0.0 29 0.0 0.0 0.0 0.1970894923799702 0.0 30 0.0 0.0 0.0 0.24636186547496275 0.0 31 0.0 0.0 0.0 0.3116764065543715 0.0 32 0.0 0.0 0.0 0.3850120316259883 0.0 33 0.0 0.0 0.0 0.4835567778159734 0.0 34 0.0 0.0 0.0 0.6187693365417669 0.0 35 0.0 0.0 0.0 0.8181505672052252 0.0 36 0.0 0.0 0.0 1.1435774034605248 0.0 37 0.0 0.0 0.0 1.522860089377793 0.0 38 0.0 0.0 0.0 1.9365188495473817 0.0 39 0.0 0.0 0.0 2.303196974905466 0.0 40 0.0 0.0 0.0 2.763836369886559 0.0 41 0.0 0.0 0.0 3.293227913372293 0.0 42 0.0 0.0 0.0 3.7779305603300104 0.0 43 0.0 0.0 0.0 4.305030365532256 0.0 44 0.0 0.0 0.0 4.8458806004354305 0.0 45 0.0 0.0 0.0 5.37985562048814 0.0 46 0.0 0.0 0.0 5.967686490202819 0.0 47 0.0 0.0 0.0 6.4913486879798326 0.0 48 0.0 0.0 0.0 7.034490661166495 0.0 49 0.0 0.0 0.0 7.51575570069898 0.0 50 0.0 0.0 0.0 7.976395095680074 0.0 51 0.0 0.0 0.0 8.494327947748367 0.0 52 0.0 0.0 0.0 9.016844276383637 0.0 53 0.0 0.0 0.0 9.549673427294604 0.0 54 0.0 0.0 0.0 10.068752148504641 0.0 55 0.0 0.0 0.0 10.563767617738055 0.0 56 0.0 0.0 0.0 11.030136358427868 0.0 57 0.0 0.0 0.0 11.503380313968146 0.0 58 0.0 0.0 0.0 11.968603185516214 0.0 59 0.0 0.0 0.0 12.452159963332187 0.0 60 0.0 0.0 0.0 12.93571674114816 0.0 61 0.0 0.001145869141744013 0.0 13.42958634123983 0.0 62 0.0 0.001145869141744013 0.0 13.924601810473243 0.0 63 0.0 0.001145869141744013 0.0 14.45170161567549 0.0 64 0.0 0.001145869141744013 0.0 14.953592299759368 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACGGG 15 0.0022180013 70.0 63 ATCCTTC 20 0.006930632 52.5 59 AAGTAAT 20 0.006930632 52.5 57 GAAGCGA 20 0.006930632 52.5 42 TTATGCC 20 0.006930632 52.5 46 ACTTCGC 20 0.006930632 52.5 65 GCTCTAC 20 0.006930632 52.5 1 TGGTCTA 20 0.006930632 52.5 43 ATCTCAT 30 5.849418E-4 46.666664 23 CGACGAA 35 0.0012494029 40.0 22 CTTTGAG 35 0.0012494029 40.0 32 CTGCAGC 55 6.6283537E-6 38.18182 1 CATTAAG 40 0.0024072418 35.0 53 CCTTTGA 40 0.0024072418 35.0 31 AAAAAGA 45 0.004286885 31.111109 51 CGCCAAC 45 0.004286885 31.111109 21 TAGATCT 70 3.4543933E-5 30.0 39 TTGGCTT 50 0.007174478 27.999998 4 GACAACA 50 0.007174478 27.999998 40 GTTAGGA 50 0.007174478 27.999998 3 >>END_MODULE