FastQCFastQC Report
Wed 25 May 2016
SRR1780190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87270
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT29513.3814598372865814No Hit
CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG17962.057980978572247TruSeq Adapter, Index 12 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG7570.8674229403002177No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6530.7482525495588404No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2810.3219892288300676No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT2240.2566746877506589TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT990.11344104503265727No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT920.10541996104044918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAA150.002218001370.028
TCTCTCT150.002218001370.024
CAAGGTT150.002218001370.032
CTGCAAG150.002218001370.029
CTCTGCA150.002218001370.027
AATCGTC150.002218001370.028
CTTAATA200.00693063252.52
TTAATAC200.00693063252.53
TGGACTA200.00693063252.570
CTCCAAT200.00693063252.512
GTGTCAG200.00693063252.51
TTTGGAC200.00693063252.568
AGTGTCC200.00693063252.546
CTAATCG200.00693063252.526
TGCAAGG200.00693063252.530
TCCTCTT305.849418E-446.66666463
AAAACGA305.849418E-446.66666470
CATACTT305.849418E-446.6666645
TCTTTAT604.8512447E-946.6666641
CTTTATA757.403287E-1042.0000042
Produced by FastQC (version 0.11.5)