##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780190_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87270 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.450429700928154 34.0 34.0 34.0 34.0 34.0 2 33.54260341469004 34.0 34.0 34.0 34.0 34.0 3 33.59915205683511 34.0 34.0 34.0 34.0 34.0 4 33.64538787670448 34.0 34.0 34.0 34.0 34.0 5 33.61410564913487 34.0 34.0 34.0 34.0 34.0 6 37.20127191474734 38.0 38.0 38.0 36.0 38.0 7 37.19649364042626 38.0 38.0 38.0 36.0 38.0 8 36.997249914059815 38.0 37.0 38.0 35.0 38.0 9 37.07088346510829 38.0 38.0 38.0 36.0 38.0 10-11 36.98270883465108 38.0 37.0 38.0 35.0 38.0 12-13 36.955018906840834 38.0 37.0 38.0 35.0 38.0 14-15 36.86151025552881 38.0 37.0 38.0 35.0 38.0 16-17 36.88704594935258 38.0 37.0 38.0 34.5 38.0 18-19 36.848229632176 38.0 37.0 38.0 34.5 38.0 20-21 36.882594247736904 38.0 37.0 38.0 35.0 38.0 22-23 36.814162942591956 38.0 37.0 38.0 34.0 38.0 24-25 36.83973301248997 38.0 37.0 38.0 34.5 38.0 26-27 36.72156525724762 38.0 37.0 38.0 34.0 38.0 28-29 36.66310874298155 38.0 37.0 38.0 34.0 38.0 30-31 36.62935143806577 38.0 37.0 38.0 34.0 38.0 32-33 36.567932852068296 38.0 37.0 38.0 34.0 38.0 34-35 36.571473587716284 38.0 37.0 38.0 34.0 38.0 36-37 36.60143806577289 38.0 37.0 38.0 34.0 38.0 38-39 36.20565486421451 38.0 37.0 38.0 34.0 38.0 40-41 36.383608341927356 38.0 37.0 38.0 34.0 38.0 42-43 36.48257133035408 38.0 37.0 38.0 34.0 38.0 44-45 36.56697032198923 38.0 37.0 38.0 34.0 38.0 46-47 36.46176807608571 38.0 37.0 38.0 34.0 38.0 48-49 36.490941904434514 38.0 37.0 38.0 34.0 38.0 50-51 36.26827661281082 38.0 37.0 38.0 34.0 38.0 52-53 36.33379168099003 38.0 37.0 38.0 34.0 38.0 54-55 36.231093159161226 38.0 37.0 38.0 34.0 38.0 56-57 36.2886329781139 38.0 37.0 38.0 34.0 38.0 58-59 36.14403002177151 38.0 37.0 38.0 34.0 38.0 60-61 36.16725678927467 38.0 37.0 38.0 34.0 38.0 62-63 36.116236965738516 38.0 37.0 38.0 33.5 38.0 64-65 36.020745960811276 38.0 37.0 38.0 32.5 38.0 66-67 35.59308467972957 38.0 36.5 38.0 31.0 38.0 68-69 35.53697719720408 38.0 36.0 38.0 31.0 38.0 70-71 35.07568465681219 38.0 36.0 38.0 27.0 38.0 72-73 34.55585539131431 38.0 36.0 38.0 24.0 38.0 74-75 34.36651770367824 38.0 35.5 38.0 23.0 38.0 76 33.45056720522516 37.0 34.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17080384597400666 1101 2 0.10302595904574474 1101 3 0.17399607439070053 1101 4 0.17465441598312736 1101 5 -0.05868776044530222 1101 6 0.14183620587402856 1101 7 0.34717551438078686 1101 8 0.21881726690369874 1101 9 0.43092334835245083 1101 10-11 0.3406443141076565 1101 12-13 0.47354519285558894 1101 14-15 0.5266009627785309 1101 16-17 0.3593397400923095 1101 18-19 0.5026505307288431 1101 20-21 0.2669618662050226 1101 22-23 0.39918211331772113 1101 24-25 0.39183633759749625 1101 26-27 0.19448066214667392 1101 28-29 0.23339516101273006 1101 30-31 0.13803459207768043 1101 32-33 0.0773592790488351 1101 34-35 0.3862346971885202 1101 36-37 0.6344881374279723 1101 38-39 0.6080443013848438 1101 40-41 0.3683505851714912 1101 42-43 0.29272142827403513 1101 44-45 0.15371418729108655 1101 46-47 0.39830894887673907 1101 48-49 0.6830940352308588 1101 50-51 0.6014005240342826 1101 52-53 0.4014911513174795 1101 54-55 0.5747104163632528 1101 56-57 0.4233061224894854 1101 58-59 0.2867462057122623 1101 60-61 0.13458717187821634 1101 62-63 0.48835261697092136 1101 64-65 0.1383392155629295 1101 66-67 0.6089675960285561 1101 68-69 0.17974393019154888 1101 70-71 0.5923349495747487 1101 72-73 0.5573384633522025 1101 74-75 0.22958018256474588 1101 76 -0.4729513696613239 1102 1 0.22611743482418234 1102 2 -0.01970923259188595 1102 3 -0.16076002317027616 1102 4 -0.023690531752059485 1102 5 -0.13142295208293575 1102 6 0.21474561353953447 1102 7 0.03619990462468792 1102 8 0.045907859238191406 1102 9 -0.1635017387555635 1102 10-11 0.19682898309720542 1102 12-13 0.2550782939008869 1102 14-15 0.2998152484928127 1102 16-17 0.22475785855920094 1102 18-19 0.30596063525845807 1102 20-21 0.2765001937311524 1102 22-23 0.19861591122712952 1102 24-25 0.24762902052432878 1102 26-27 0.12226811859265752 1102 28-29 0.5323063108385142 1102 30-31 0.14718093354110096 1102 32-33 0.37235056824743396 1102 34-35 0.3932904463174438 1102 36-37 0.3813958029331985 1102 38-39 0.2878352421513952 1102 40-41 0.3143871705373442 1102 42-43 0.38527369308240367 1102 44-45 0.39243369948621876 1102 46-47 0.437289785113677 1102 48-49 0.48919159620647434 1102 50-51 0.5311479107938695 1102 52-53 0.5460904544533633 1102 54-55 0.35615640939460746 1102 56-57 0.42326256848252086 1102 58-59 0.3781225123324674 1102 60-61 0.45131191055417474 1102 62-63 0.2061226518141268 1102 64-65 0.4094629089427215 1102 66-67 0.7415459932410968 1102 68-69 0.6498658814110598 1102 70-71 0.5389812910381551 1102 72-73 0.1197513553382592 1102 74-75 -0.13222295332374756 1102 76 -0.2158956205324074 1103 1 0.040422241277333626 1103 2 0.15013946198116912 1103 3 0.13392758124001602 1103 4 0.05082858428058756 1103 5 0.23705587947641504 1103 6 0.044037771431760575 1103 7 0.1260404850265786 1103 8 0.5987096348097651 1103 9 0.09716600980058843 1103 10-11 0.4911824745773288 1103 12-13 0.42344734549746477 1103 14-15 0.4201674989820532 1103 16-17 0.34931038588486274 1103 18-19 0.4329832117464605 1103 20-21 0.35152644546138134 1103 22-23 0.3158895497169354 1103 24-25 0.30614651372273727 1103 26-27 0.3788250848472572 1103 28-29 0.33733351717710747 1103 30-31 0.341067860179443 1103 32-33 0.45942875067309785 1103 34-35 0.2814401766405794 1103 36-37 0.48397443879783 1103 38-39 0.5215472368055885 1103 40-41 0.7346177084591616 1103 42-43 0.76715880596484 1103 44-45 0.34483453954157994 1103 46-47 0.2682942717730228 1103 48-49 0.6279081252504355 1103 50-51 0.42774592520845545 1103 52-53 0.45946571100436984 1103 54-55 0.4943777353456369 1103 56-57 0.5492112105521088 1103 58-59 0.6728518808590351 1103 60-61 0.4886967609193178 1103 62-63 0.5402117167752252 1103 64-65 0.6223167106705603 1103 66-67 0.6444861870265939 1103 68-69 1.1609572374322568 1103 70-71 1.0738662607293463 1103 72-73 1.3770302441741151 1103 74-75 1.1556809653436133 1103 76 1.45527759315668 1104 1 0.24929221806702628 1104 2 0.30344124143777407 1104 3 0.14002597472292422 1104 4 0.14550159206286395 1104 5 0.30136207344173727 1104 6 0.38361361451190135 1104 7 0.3380393017562042 1104 8 0.0896644429834339 1104 9 0.4947107237677315 1104 10-11 0.3844150782272635 1104 12-13 0.3237113058124024 1104 14-15 0.16028036477188579 1104 16-17 0.36141615205244193 1104 18-19 0.1991621586358221 1104 20-21 0.5683322980987171 1104 22-23 0.20586815982935036 1104 24-25 0.4029243774646005 1104 26-27 0.40486272194733175 1104 28-29 0.2628386826074163 1104 30-31 0.4249947249680517 1104 32-33 0.5291449933345405 1104 34-35 0.3200386838994902 1104 36-37 0.31916421716219645 1104 38-39 0.5724961286273711 1104 40-41 0.34189709679939995 1104 42-43 0.5109523252840091 1104 44-45 0.580749071012022 1104 46-47 0.22409798542159365 1104 48-49 0.5420641348986308 1104 50-51 0.4436347433033845 1104 52-53 0.39484663304505574 1104 54-55 0.0973848349678974 1104 56-57 0.007899145745298597 1104 58-59 0.5796864050478092 1104 60-61 0.5654841818450009 1104 62-63 0.5498144109908552 1104 64-65 0.44211828533037334 1104 66-67 0.32375164918470034 1104 68-69 0.4231824683975276 1104 70-71 0.4081572087109606 1104 72-73 1.0581274998970471 1104 74-75 0.8026283553222271 1104 76 0.49490577319581774 1105 1 -0.02922012334526869 1105 2 0.19744110765552847 1105 3 0.2040296314376775 1105 4 0.10339362978945843 1105 5 0.09243879756044748 1105 6 0.25480360759981835 1105 7 0.16141329002795857 1105 8 0.21677987476563487 1105 9 0.4420451124943483 1105 10-11 0.5400930197644129 1105 12-13 0.47709265210774987 1105 14-15 0.4481853347823659 1105 16-17 0.5308402194786908 1105 18-19 0.4958911826943293 1105 20-21 0.3929326236355095 1105 22-23 0.3077990835957607 1105 24-25 0.3606882072043973 1105 26-27 0.3639197800757188 1105 28-29 0.2485797247711119 1105 30-31 0.4188351673413493 1105 32-33 0.5265083682146923 1105 34-35 0.5887514757120087 1105 36-37 0.4015183387702521 1105 38-39 0.40540767626499274 1105 40-41 0.5367230683929662 1105 42-43 0.03870656729608868 1105 44-45 0.31297832918946256 1105 46-47 0.46560281273100657 1105 48-49 0.3956060198904936 1105 50-51 0.35623034186746594 1105 52-53 0.6284806048169997 1105 54-55 0.17391942882729694 1105 56-57 0.2954417888365626 1105 58-59 0.4490424664984545 1105 60-61 0.9335804604688249 1105 62-63 0.5545079381598015 1105 64-65 0.39079607078824097 1105 66-67 0.29540096132098626 1105 68-69 0.7788091267399722 1105 70-71 0.4343172122784864 1105 72-73 0.7596035640233438 1105 74-75 0.8759967693336819 1105 76 1.1229019381047323 1106 1 0.11997967014983146 1106 2 -0.06348253246274282 1106 3 0.07487020026482583 1106 4 0.05899507532379289 1106 5 0.048579971822427126 1106 6 0.23002301906084455 1106 7 0.16261007981535158 1106 8 0.3253357484221766 1106 9 0.39515911258821035 1106 10-11 0.40875120721455005 1106 12-13 0.33083790014829617 1106 14-15 0.3577573064348698 1106 16-17 0.4678562236087913 1106 18-19 0.3954077734860917 1106 20-21 0.44999133673449165 1106 22-23 0.34879500043060574 1106 24-25 0.37693873519989296 1106 26-27 0.354145996812008 1106 28-29 0.24624481136238074 1106 30-31 -0.01443793539484517 1106 32-33 0.45771642190597106 1106 34-35 0.05389203984586999 1106 36-37 0.44751261295244404 1106 38-39 0.19675559009613153 1106 40-41 0.4145918439127527 1106 42-43 0.3076440056966163 1106 44-45 0.24690849298539774 1106 46-47 0.4578119458797403 1106 48-49 0.6217054238422506 1106 50-51 0.2382761484099163 1106 52-53 0.5123802622065909 1106 54-55 0.3457643624171993 1106 56-57 0.3363555730389365 1106 58-59 0.5266812706473303 1106 60-61 0.829342416633466 1106 62-63 0.5882527168710254 1106 64-65 0.5995654892892048 1106 66-67 0.14982673688769665 1106 68-69 0.46473394018156 1106 70-71 0.3573824021221981 1106 72-73 0.4364468549605931 1106 74-75 0.2845127499973188 1106 76 1.2666340449240892 1107 1 -0.20140001777789962 1107 2 -0.11620383251500499 1107 3 0.22479678613446907 1107 4 0.08979168085349443 1107 5 0.15642408500461613 1107 6 0.10519411131378575 1107 7 0.20317449760396755 1107 8 0.0903856451446714 1107 9 0.4260428196285062 1107 10-11 0.2312009994152362 1107 12-13 0.14654976835127087 1107 14-15 0.44546852759805233 1107 16-17 0.39969307003637766 1107 18-19 0.07639226941719812 1107 20-21 0.047713010078943796 1107 22-23 0.23494088301777083 1107 24-25 0.25971254501996555 1107 26-27 0.050746549284440334 1107 28-29 0.34767452044332714 1107 30-31 0.12996392608886964 1107 32-33 0.016435283115939114 1107 34-35 0.09112793562268706 1107 36-37 0.2762586000614249 1107 38-39 0.15334170860395346 1107 40-41 0.33556588766259665 1107 42-43 0.21725752385106034 1107 44-45 0.30267834590827647 1107 46-47 0.2174179981904203 1107 48-49 0.4781372482456021 1107 50-51 0.39745415901141 1107 52-53 0.24236557938051817 1107 54-55 0.17169821504143812 1107 56-57 -0.10567837166404814 1107 58-59 0.61629628197052 1107 60-61 0.3043380615579352 1107 62-63 0.1731518035494659 1107 64-65 0.5472423675710587 1107 66-67 0.711687474015342 1107 68-69 0.8502701121946004 1107 70-71 0.10971547829869621 1107 72-73 0.34199148826323267 1107 74-75 0.05147265841630144 1107 76 -0.35461125781587555 1108 1 0.1465738937700749 1108 2 0.14629467917320227 1108 3 0.14788083275501407 1108 4 0.05685037987054642 1108 5 -0.1475751741468443 1108 6 -0.13972247707870622 1108 7 0.217462290801393 1108 8 0.1927285786402848 1108 9 0.3606100185595622 1108 10-11 -0.14155164977975687 1108 12-13 -0.10733637910404781 1108 14-15 -0.22142744932980918 1108 16-17 0.03505668114381422 1108 18-19 0.13300103524291984 1108 20-21 0.2416764174530286 1108 22-23 0.26412502356732404 1108 24-25 0.06988333706642891 1108 26-27 0.15277859098362967 1108 28-29 0.38306988220231375 1108 30-31 0.2140436202242597 1108 32-33 0.17147106874612206 1108 34-35 0.0653239165193753 1108 36-37 0.10209966159153794 1108 38-39 -0.11505713249726313 1108 40-41 0.1577093212309677 1108 42-43 0.19331224080723075 1108 44-45 -0.10631634908702381 1108 46-47 0.37836072795268905 1108 48-49 0.021463658172969247 1108 50-51 0.08243477788452935 1108 52-53 0.24110878275666892 1108 54-55 0.2241926256038198 1108 56-57 0.2730870571681905 1108 58-59 0.21002023651417545 1108 60-61 0.42566523879271756 1108 62-63 0.10837385966874535 1108 64-65 0.5661472346813525 1108 66-67 0.744628774998283 1108 68-69 0.30577951171040496 1108 70-71 0.7197579447951412 1108 72-73 0.18052619569420614 1108 74-75 0.23898671469433452 1108 76 0.561289203891512 1109 1 0.15573688168829136 1109 2 0.09320453047431698 1109 3 0.2497773243906991 1109 4 0.1876008445545594 1109 5 0.19633456098326718 1109 6 -0.29131111555454225 1109 7 0.10554605009507156 1109 8 0.055312802617983436 1109 9 0.35246352692387717 1109 10-11 0.14566663553623016 1109 12-13 0.08783090714130282 1109 14-15 0.15807417706424332 1109 16-17 0.10292232937801771 1109 18-19 0.15376660215741822 1109 20-21 0.2927989197764518 1109 22-23 0.15559952403200583 1109 24-25 0.18301937331177953 1109 26-27 0.15675744786095436 1109 28-29 0.2560514110127272 1109 30-31 0.21978682422054163 1109 32-33 0.10420079690597106 1109 34-35 0.11162532218852306 1109 36-37 0.018863137427970855 1109 38-39 -0.13018114504372846 1109 40-41 0.10116308517149264 1109 42-43 0.48580178541689634 1109 44-45 0.13373650871966447 1109 46-47 0.30232680601960027 1109 48-49 0.4281275173737171 1109 50-51 0.276846952605716 1109 52-53 0.2926741870317642 1109 54-55 0.05289121993467916 1109 56-57 0.15561639034662988 1109 58-59 0.31805200928369004 1109 60-61 0.06505592187821918 1109 62-63 0.13958029554235196 1109 64-65 0.5244999298486448 1109 66-67 0.34630018531427 1109 68-69 0.32611669804869337 1109 70-71 0.33954477100331815 1109 72-73 -0.7422709116478003 1109 74-75 0.26339714685046545 1109 76 -0.024067441089897557 1110 1 -0.011637761847829609 1110 2 0.06979673579751022 1110 3 0.1296044394097109 1110 4 0.03925246052413911 1110 5 0.14819708474752247 1110 6 -0.2686608066191809 1110 7 -0.03726229115967783 1110 8 0.2550068377890895 1110 9 0.41310694422426053 1110 10-11 -0.026995555528408488 1110 12-13 -0.012929549132458362 1110 14-15 -0.1993699769281534 1110 16-17 0.042731918256343704 1110 18-19 -0.08010340734828958 1110 20-21 -0.0016625227140494303 1110 22-23 -0.01305987473225656 1110 24-25 0.06352971076425717 1110 26-27 -0.09024231449646436 1110 28-29 0.28505865911159134 1110 30-31 0.27702155568442066 1110 32-33 0.07178486297060971 1110 34-35 0.07910541962198181 1110 36-37 0.09746342259907692 1110 38-39 -0.23562761605133886 1110 40-41 -0.09509998517071239 1110 42-43 -0.015297311541274894 1110 44-45 0.12671119978430312 1110 46-47 0.05654496191313996 1110 48-49 0.11495172127105491 1110 50-51 -0.09536074701405539 1110 52-53 0.14918262334354893 1110 54-55 -0.12038735658623523 1110 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-0.08786894511429466 2116 6 -0.4594704143350299 2116 7 -0.17808580966102738 2116 8 0.08075106481310712 2116 9 0.13266550863119164 2116 10-11 0.029146056267933318 2116 12-13 -0.0859495806635735 2116 14-15 -0.0033206400089298427 2116 16-17 -0.7678379602561307 2116 18-19 -0.6295794344279528 2116 20-21 -0.13769841254062243 2116 22-23 -0.17638408877287048 2116 24-25 -0.3629981571760226 2116 26-27 -0.2161465155536746 2116 28-29 -0.06569020484093357 2116 30-31 -0.32799328248677995 2116 32-33 -0.34716162687451657 2116 34-35 0.15330786792022622 2116 36-37 0.13052472279382243 2116 38-39 -0.1786281724827532 2116 40-41 -0.5270762440968042 2116 42-43 -0.4187332755587079 2116 44-45 -0.4263816315242366 2116 46-47 0.11063473284887237 2116 48-49 -0.3030557905531097 2116 50-51 -0.5855768278820861 2116 52-53 0.11098592483664049 2116 54-55 -0.2723279111628827 2116 56-57 -0.4774342956289743 2116 58-59 -0.11577992669191417 2116 60-61 -0.016022584219335556 2116 62-63 -0.06173449104301909 2116 64-65 -0.3348750701513552 2116 66-67 -0.880405226271094 2116 68-69 -0.5792281952439851 2116 70-71 -0.130443796069855 2116 72-73 -1.2021127561599982 2116 74-75 0.2789268724602181 2116 76 -0.2864531118216078 2117 1 -0.028070909407595934 2117 2 -0.30876464760787314 2117 3 0.2298179921989174 2117 4 0.1227463924997636 2117 5 0.16171341372299253 2117 6 -0.6271951052805704 2117 7 -0.6171101999049284 2117 8 -1.30882461176558 2117 9 -0.5363956340350242 2117 10-11 -0.42662574460075575 2117 12-13 -0.5662786818997958 2117 14-15 -0.43257222704534826 2117 16-17 -0.1883608469918414 2117 18-19 -0.25194829510285643 2117 20-21 -0.3590899928947806 2117 22-23 -0.17726113521457165 2117 24-25 -0.6938534862772627 2117 26-27 -0.11946258638562313 2117 28-29 -0.7328719622749418 2117 30-31 -0.08872666036850063 2117 32-33 -0.5046123452173106 2117 34-35 -0.8504091384279135 2117 36-37 -0.5382858351747686 2117 38-39 -0.11201440703002419 2117 40-41 -0.1968891408559017 2117 42-43 0.0770903128141569 2117 44-45 -0.05927504949951867 2117 46-47 -0.15122627617218143 2117 48-49 -0.632365847352311 2117 50-51 -0.4095828419148333 2117 52-53 -0.4566301794065879 2117 54-55 0.4926654065785101 2117 56-57 -0.5535285154752856 2117 58-59 -0.6062577081820635 2117 60-61 -0.1859607733272597 2117 62-63 -0.5556786941836762 2117 64-65 -1.0386421934390242 2117 66-67 -0.6671576485898427 2117 68-69 -0.6181523516088419 2117 70-71 -0.41757209543504104 2117 72-73 -0.6640656462368426 2117 74-75 0.08205082835731048 2117 76 -0.34266761232277076 2118 1 0.03313103491409919 2118 2 -0.5569970824289072 2118 3 -0.8816490868996212 2118 4 -0.2524193167357609 2118 5 -0.2170978986941492 2118 6 -0.03362462361906182 2118 7 -0.17356181280815264 2118 8 -0.8704684473820166 2118 9 -0.4646987714632189 2118 10-11 -0.7274432434960261 2118 12-13 -0.22668522189095341 2118 14-15 -0.005484290677692627 2118 16-17 -0.7005680940090784 2118 18-19 -0.2880176720361334 2118 20-21 -0.18067638264290053 2118 22-23 -0.5630874719357379 2118 24-25 -0.5252719573333877 2118 26-27 -0.029482471493878393 2118 28-29 0.2773467739159514 2118 30-31 -0.5975644862633303 2118 32-33 -0.2721222877714453 2118 34-35 -0.37747498023083637 2118 36-37 -0.08043121741074089 2118 38-39 -0.1029003990759847 2118 40-41 -0.09414941482850736 2118 42-43 -0.21908732748632787 2118 44-45 -0.5115507896674316 2118 46-47 -0.746564323012656 2118 48-49 -0.27669859149725085 2118 50-51 -0.6268828861039637 2118 52-53 -0.4539235952262999 2118 54-55 -0.4257504131298333 2118 56-57 -0.439078670137242 2118 58-59 -0.6540372044259897 2118 60-61 -0.6371870216701652 2118 62-63 0.16692404554235196 2118 64-65 -1.0485847475707146 2118 66-67 -0.042875721943794076 2118 68-69 -0.7814311850158191 2118 70-71 -0.7455106725450662 2118 72-73 -0.7414518592284409 2118 74-75 0.27007557426982487 2118 76 -0.7435734894769936 2119 1 0.006296892750235372 2119 2 -0.32355431908320753 2119 3 0.14811111863848936 2119 4 0.08043823393509086 2119 5 -0.045887507600802735 2119 6 -0.057247232811178606 2119 7 -0.4533933857456347 2119 8 -0.3660436686209536 2119 9 -1.214841506261962 2119 10-11 -0.3775288843752733 2119 12-13 -0.3369478539206412 2119 14-15 -1.0149761547941623 2119 16-17 -0.05801171376357672 2119 18-19 -0.5104341721788614 2119 20-21 -1.0366569707102755 2119 22-23 -0.32782483990605016 2119 24-25 -0.770347606776717 2119 26-27 -0.07976080435143729 2119 28-29 -0.4328659009341749 2119 30-31 -0.3377871525493674 2119 32-33 -0.6927260559701409 2119 34-35 -0.684783001926526 2119 36-37 -0.6961534554923787 2119 38-39 -0.017366570928686542 2119 40-41 -0.15830870686390597 2119 42-43 -0.22237022785743932 2119 44-45 -0.1554988342006851 2119 46-47 -0.7108092559273018 2119 48-49 -0.0646303642634507 2119 50-51 0.07565779331367395 2119 52-53 -0.4632370408443407 2119 54-55 -0.4549039957732859 2119 56-57 -0.1947880620869924 2119 58-59 -0.6406558170437435 2119 60-61 -0.4530234475908017 2119 62-63 -0.2246688748116341 2119 64-65 -0.2474856896203761 2119 66-67 -0.8232068666768768 2119 68-69 -0.6988591039424534 2119 70-71 0.002812193569688759 2119 72-73 0.2439535075999899 2119 74-75 0.30083492339913676 2119 76 -0.40522064905449895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 5.0 21 2.0 22 7.0 23 13.0 24 40.0 25 79.0 26 133.0 27 273.0 28 436.0 29 737.0 30 1202.0 31 1699.0 32 2712.0 33 4458.0 34 6849.0 35 9563.0 36 15594.0 37 43467.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.610862839463735 18.60662312363928 12.745502463618655 31.037011573278335 2 20.73908559642489 20.59126847713991 33.68969863641572 24.97994729001948 3 20.187922539246017 20.049272373094993 29.618425575799247 30.144379511859746 4 16.088002750085938 21.627134181276496 32.456743439899164 29.8281196287384 5 16.152171422023606 31.809327374813794 36.17738054314197 15.861120660020624 6 30.62449868225049 35.029219663114475 16.409991978916008 17.936289675719035 7 28.96298842672167 25.497880142087777 22.299759367480235 23.239372063710324 8 24.527328979030592 34.019708949237994 17.491692448722358 23.961269623009052 9 23.94408158588289 15.855391314311905 18.544746189985105 41.6557809098201 10-11 27.961498796837404 25.36667812535808 23.394637332416636 23.277185745387875 12-13 26.971467858370573 19.553111034719837 24.091325770596995 29.384095336312594 14-15 23.89194453993354 19.80061876933654 20.59928956113212 35.7081471295978 16-17 27.116420304801192 25.23719491234101 20.61475879454566 27.031625988312136 18-19 28.584278675375273 23.726939383522403 21.460983155723618 26.22779878537871 20-21 25.336312593101866 22.935143806577287 21.49020281883809 30.238340781482755 22-23 24.81494213360834 24.29471754325656 22.286008937779307 28.604331385355792 24-25 26.851724533058324 25.492723730949923 21.031855162140484 26.62369657385127 26-27 26.939956456972613 25.193078950383867 23.941216913028533 23.92574767961499 28-29 25.939039761659217 23.901111493067493 24.145181620258967 26.014667125014324 30-31 24.219090179901457 25.595851953706887 23.31843703449066 26.866620831900995 32-33 23.672510599289563 22.90707001260456 27.50085940185631 25.919559986249567 34-35 26.760627936289676 23.639853328749858 25.26011229517589 24.33940643978458 36-37 26.05305374126275 27.17371376188839 22.7466483327604 24.02658416408846 38-39 23.552767273977313 24.8997364500974 25.897215537985563 25.650280737939728 40-41 24.736450097398876 21.261028990489287 26.0192506015813 27.98327031053054 42-43 27.300905236621976 20.94534204193881 25.403345937893896 26.35040678354532 44-45 23.301821931935372 24.007677323249684 27.331270768878195 25.35922997593675 46-47 25.621061074825256 21.711928497765555 26.656353844390974 26.01065658301822 48-49 25.2538100148963 21.06852297467629 24.501546923341355 29.176120087086055 50-51 24.661968603185517 21.5452045376418 25.819869370917843 27.972957488254842 52-53 21.984645353500632 21.503953248539016 31.055345479546236 25.456055918414116 54-55 22.98441617967228 22.419502692792484 27.30434284404721 27.291738283488026 56-57 24.30044688896528 23.754440242924257 24.572590810129483 27.37252205798098 58-59 22.716855735075054 23.90855964248883 27.473358542454452 25.901226079981665 60-61 24.395554027730032 26.070814712959777 24.407585653718343 25.12604560559184 62-63 21.945685802681332 30.104847026469578 22.536381345250373 25.41308582559872 64-65 21.575570069898017 31.321760055001718 21.974332531224935 25.12833734387533 66-67 21.86432909361751 30.17703678239945 21.971467858370573 25.987166265612466 68-69 21.25071616821359 30.550590122607996 22.217829723845536 25.980863985332874 70-71 21.58989343416982 29.527901913601468 22.499713532714562 26.38249111951415 72-73 21.49077575340896 27.791337229288416 22.87269393835224 27.845193078950388 74-75 21.145869141744015 27.35590695542569 23.653603758450785 27.844620144379512 76 21.33952102669875 27.696803025094535 23.231350979718115 27.732324968488598 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.5 17 3.0 18 5.5 19 8.0 20 10.0 21 12.0 22 14.5 23 22.0 24 50.0 25 73.0 26 117.0 27 189.5 28 243.0 29 268.0 30 378.5 31 575.0 32 782.0 33 903.0 34 1052.5 35 1397.0 36 1773.5 37 1955.0 38 2845.0 39 4478.5 40 5370.5 41 5305.0 42 5091.0 43 4786.5 44 4520.0 45 4522.0 46 4486.0 47 4412.0 48 4135.0 49 3627.5 50 3323.0 51 3078.0 52 2541.5 53 2036.5 54 1823.0 55 1805.0 56 1618.0 57 1395.0 58 1341.0 59 1228.0 60 1123.0 61 1147.0 62 1163.0 63 1197.5 64 1324.0 65 1510.5 66 1645.5 67 1686.0 68 1761.0 69 1904.5 70 1998.0 71 2023.0 72 1988.0 73 1830.5 74 1619.5 75 1531.0 76 1329.0 77 1000.0 78 716.5 79 560.0 80 436.5 81 230.0 82 109.5 83 72.0 84 50.5 85 18.0 86 4.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 87270.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.618310988885064 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98214285714286 37.3496046751461 2 15.191637630662022 15.987166265612467 3 5.298344947735192 8.36369886558955 4 2.7155923344947737 5.715595279019136 5 1.7399825783972125 4.577747221267332 6 1.093205574912892 3.451357854932967 7 0.7556620209059234 2.783316145296207 8 0.5052264808362369 2.1267331270768874 9 0.3789198606271777 1.794431075971124 >10 1.3109756097560976 9.636759482067148 >50 0.01524390243902439 0.5798097857224704 >100 0.004355400696864111 0.5786639165807265 >500 0.004355400696864111 1.615675489859058 >1k 0.004355400696864111 5.439440815858829 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 2951 3.3814598372865814 No Hit CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG 1796 2.057980978572247 TruSeq Adapter, Index 12 (96% over 26bp) TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 757 0.8674229403002177 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 653 0.7482525495588404 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 281 0.3219892288300676 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 224 0.2566746877506589 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT 99 0.11344104503265727 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT 92 0.10541996104044918 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0034376074252320385 0.0 2 0.0 0.0 0.0 0.005729345708720064 0.0 3 0.0 0.0 0.0 0.005729345708720064 0.0 4 0.0 0.0 0.0 0.01260456055918414 0.0 5 0.0 0.0 0.0 0.01604216798441618 0.0 6 0.0 0.0 0.0 0.01604216798441618 0.0 7 0.0 0.0 0.0 0.017188037126160193 0.0 8 0.0 0.0 0.0 0.017188037126160193 0.0 9 0.0 0.0 0.0 0.022917382834880255 0.0 10 0.0 0.0 0.0 0.022917382834880255 0.0 11 0.0 0.0 0.0 0.022917382834880255 0.0 12 0.0 0.0 0.0 0.022917382834880255 0.0 13 0.0 0.0 0.0 0.022917382834880255 0.0 14 0.0 0.0 0.0 0.02406325197662427 0.0 15 0.0 0.0 0.0 0.02406325197662427 0.0 16 0.0 0.0 0.0 0.026354990260112295 0.0 17 0.0 0.0 0.0 0.026354990260112295 0.0 18 0.0 0.0 0.0 0.02864672854360032 0.0 19 0.0 0.0 0.0 0.030938466827088347 0.0 20 0.0 0.0 0.0 0.03666781253580841 0.0 21 0.0 0.0 0.0 0.03666781253580841 0.0 22 0.0 0.0 0.0 0.04010541996104045 0.0 23 0.0 0.0 0.0 0.043543027386272484 0.0 24 0.0 0.0 0.0 0.046980634811504524 0.0 25 0.0 0.001145869141744013 0.0 0.04927237309499255 0.0 26 0.0 0.001145869141744013 0.0 0.058439326228944655 0.0 27 0.0 0.001145869141744013 0.0 0.09166953133952102 0.0 28 0.0 0.001145869141744013 0.0 0.13521255872579352 0.0 29 0.0 0.001145869141744013 0.0 0.18563080096253007 0.0 30 0.0 0.001145869141744013 0.0 0.2326114357740346 0.0 31 0.0 0.001145869141744013 0.0 0.2933425002864673 0.0 32 0.0 0.001145869141744013 0.0 0.35980291050762003 0.0 33 0.0 0.001145869141744013 0.0 0.4572017875558611 0.0 34 0.0 0.001145869141744013 0.0 0.5878308697146786 0.0 35 0.0 0.001145869141744013 0.0 0.7814827546694167 0.0 36 0.0 0.001145869141744013 0.0 1.0943050303655322 0.0 37 0.0 0.001145869141744013 0.0 1.4667125014323363 0.0 38 0.0 0.001145869141744013 0.0 1.863183224475765 0.0 39 0.0 0.001145869141744013 0.0 2.214965050991177 0.0 40 0.0 0.001145869141744013 0.0 2.6641457545548297 0.0 41 0.0 0.001145869141744013 0.0 3.1774951300561476 0.0 42 0.0 0.001145869141744013 0.0 3.663343646155609 0.0 43 0.0 0.001145869141744013 0.0 4.180130629082159 0.0 44 0.0 0.001145869141744013 0.0 4.709522172567893 0.0 45 0.0 0.001145869141744013 0.0 5.234330239486651 0.0 46 0.0 0.001145869141744013 0.0 5.8129941560673775 0.0 47 0.0 0.001145869141744013 0.0 6.322905924143463 0.0 48 0.0 0.001145869141744013 0.0 6.843130514495245 0.0 49 0.0 0.001145869141744013 0.0 7.314082731752034 0.0 50 0.0 0.001145869141744013 0.0 7.768992781024407 0.0 51 0.0 0.001145869141744013 0.0 8.27546694167526 0.0 52 0.0 0.001145869141744013 0.0 8.796837401168787 0.0 53 0.0 0.001145869141744013 0.0 9.318207860662312 0.0 54 0.0 0.001145869141744013 0.0 9.829265497880142 0.0 55 0.0 0.001145869141744013 0.0 10.307092929987395 0.0 56 0.0 0.001145869141744013 0.0 10.760857110118025 0.0 57 0.0 0.001145869141744013 0.0 11.214621290248653 0.0 58 0.0 0.001145869141744013 0.0 11.663801993812307 0.0 59 0.0 0.001145869141744013 0.0 12.13017073450212 0.0 60 0.0 0.001145869141744013 0.0 12.60685229746763 0.0 61 0.0 0.001145869141744013 0.0 13.076658645582674 0.0 62 0.0 0.001145869141744013 0.0 13.544173255414231 0.0 63 0.0 0.001145869141744013 0.0 14.048355677781597 0.0 64 0.0 0.001145869141744013 0.0 14.547954623581987 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGCAA 15 0.0022180013 70.0 28 TCTCTCT 15 0.0022180013 70.0 24 CAAGGTT 15 0.0022180013 70.0 32 CTGCAAG 15 0.0022180013 70.0 29 CTCTGCA 15 0.0022180013 70.0 27 AATCGTC 15 0.0022180013 70.0 28 CTTAATA 20 0.006930632 52.5 2 TTAATAC 20 0.006930632 52.5 3 TGGACTA 20 0.006930632 52.5 70 CTCCAAT 20 0.006930632 52.5 12 GTGTCAG 20 0.006930632 52.5 1 TTTGGAC 20 0.006930632 52.5 68 AGTGTCC 20 0.006930632 52.5 46 CTAATCG 20 0.006930632 52.5 26 TGCAAGG 20 0.006930632 52.5 30 TCCTCTT 30 5.849418E-4 46.666664 63 AAAACGA 30 5.849418E-4 46.666664 70 CATACTT 30 5.849418E-4 46.666664 5 TCTTTAT 60 4.8512447E-9 46.666664 1 CTTTATA 75 7.403287E-10 42.000004 2 >>END_MODULE