FastQCFastQC Report
Wed 25 May 2016
SRR1780189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119047
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAACGG254.4382297E-543.9672242
TCAGATG254.4382297E-543.9672244
TGTATAA301.2965691E-436.6547611
TAGATGC250.002352891735.173785
CTTGTAC353.2146086E-431.405163
GTTCGAT300.00573357729.32380743
TTCGATT300.00573357729.32380744
CAAAGGT300.00573357729.32380730
TTGTACT300.00574537429.3114854
AAAAACG300.00574537429.3114851
TTGGTGA406.9735653E-427.51419817
AGAGACA406.9735653E-427.51419817
CGTCAGA407.0252194E-427.4795192
GAGACAG450.001386642224.45706618
AGCTTTG450.0013934624.43650444
AAAGGTA450.0013934624.43650431
CAGATGT450.001396879124.4262375
GTGTATA500.002571758621.99285310
TAGCTAC500.002571758621.99285344
GCGTCAG500.00257804821.9836121
Produced by FastQC (version 0.11.5)