##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 119047 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.962493804967785 33.0 31.0 34.0 27.0 34.0 2 31.15686241568456 34.0 31.0 34.0 27.0 34.0 3 31.246079279612253 34.0 31.0 34.0 27.0 34.0 4 34.50882424588608 37.0 35.0 37.0 30.0 37.0 5 34.61609280368258 37.0 35.0 37.0 32.0 37.0 6 34.66667786672491 37.0 35.0 37.0 31.0 37.0 7 34.67659831831126 37.0 35.0 37.0 31.0 37.0 8 34.64025972935059 37.0 35.0 37.0 31.0 37.0 9 36.331600124320644 39.0 37.0 39.0 31.0 39.0 10 36.2336136147908 39.0 37.0 39.0 31.0 39.0 11 36.30306517593891 39.0 37.0 39.0 31.0 39.0 12 36.2876090955673 39.0 37.0 39.0 31.0 39.0 13 36.22995119574622 39.0 37.0 39.0 31.0 39.0 14 37.59673910304333 40.0 38.0 41.0 31.0 41.0 15 37.58190462590405 40.0 38.0 41.0 31.0 41.0 16 37.523188320579266 40.0 38.0 41.0 31.0 41.0 17 37.465060018312094 40.0 38.0 41.0 30.0 41.0 18 37.37037472594857 40.0 38.0 41.0 30.0 41.0 19 37.317202449452736 40.0 38.0 41.0 30.0 41.0 20 37.27186741371055 40.0 38.0 41.0 30.0 41.0 21 37.24119045419036 40.0 38.0 41.0 30.0 41.0 22 37.25617613211589 40.0 38.0 41.0 30.0 41.0 23 37.15132678689929 40.0 38.0 41.0 29.0 41.0 24 37.07545759237948 40.0 38.0 41.0 29.0 41.0 25 37.033910976337076 40.0 38.0 41.0 28.0 41.0 26 36.92699522037515 40.0 38.0 41.0 28.0 41.0 27 36.85252883314993 40.0 38.0 41.0 27.0 41.0 28 36.7745428276227 40.0 38.0 41.0 27.0 41.0 29 36.70343645786958 40.0 37.0 41.0 27.0 41.0 30 36.61560560114913 40.0 37.0 41.0 27.0 41.0 31 36.55253807319798 40.0 37.0 41.0 26.0 41.0 32 36.456651574588186 40.0 37.0 41.0 26.0 41.0 33 36.346795803338175 40.0 37.0 41.0 26.0 41.0 34 36.25022050114661 40.0 36.0 41.0 25.0 41.0 35 36.140339529765555 40.0 36.0 41.0 25.0 41.0 36 36.45135114702597 40.0 37.0 41.0 26.0 41.0 37 36.46766403185296 40.0 37.0 41.0 26.0 41.0 38 36.44732752610314 40.0 36.0 41.0 26.0 41.0 39 36.401068485556124 40.0 36.0 41.0 26.0 41.0 40 36.20626307256798 40.0 35.0 41.0 25.0 41.0 41 36.169857283257876 40.0 35.0 41.0 25.0 41.0 42 36.115601401127286 40.0 35.0 41.0 24.0 41.0 43 36.05190386990012 40.0 35.0 41.0 24.0 41.0 44 35.99658118222215 40.0 35.0 41.0 24.0 41.0 45 35.928742429460634 40.0 35.0 41.0 24.0 41.0 46 35.829269112199384 40.0 35.0 41.0 24.0 41.0 47 35.702168051273866 40.0 35.0 41.0 23.0 41.0 48 35.61096037699396 40.0 35.0 41.0 23.0 41.0 49 35.52370912328744 39.0 35.0 41.0 23.0 41.0 50 35.40259729350593 39.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33699230462370267 1101 2 -0.13377282861301154 1101 3 -0.4031925213304497 1101 4 -0.4933788153136902 1101 5 -0.31620397791520105 1101 6 -0.14304978924047163 1101 7 -0.17857346068185365 1101 8 0.005785225332303412 1101 9 -0.36358837252796405 1101 10 -0.07416754949320392 1101 11 -0.11122578236609826 1101 12 -0.20919943948199915 1101 13 -0.21549910745621048 1101 14 -0.5823697975750051 1101 15 -0.7693690882001647 1101 16 -0.7190697894529521 1101 17 -0.44294431746003227 1101 18 -0.28178994956272874 1101 19 -0.5402075472528267 1101 20 -0.5013679031315448 1101 21 0.050260032330143645 1101 22 0.05379729985770609 1101 23 -0.280608542698225 1101 24 -0.14492337687842394 1101 25 -0.4784207766567192 1101 26 -0.21860838160580442 1101 27 -0.18933933607339526 1101 28 -0.038106445691603597 1101 29 -0.23327976456030086 1101 30 -0.21844038872080063 1101 31 0.02275092766922171 1101 32 -0.28349176562381473 1101 33 -0.03206632919718544 1101 34 -0.18797040536178145 1101 35 -0.17254536303116197 1101 36 -0.4387143400741067 1101 37 -0.4010077151689728 1101 38 -0.25648162147631837 1101 39 -0.3303669944078109 1101 40 -0.42081090771836926 1101 41 -0.29737792248229766 1101 42 -0.007017816274952793 1101 43 -0.0608389186229914 1101 44 -0.0886359983779883 1101 45 -0.03662181072952819 1101 46 -0.5347588435070563 1101 47 -0.10709548372597766 1101 48 -0.23250948863771015 1101 49 -0.11049596503161752 1101 50 0.14300018787240987 1102 1 -0.11702261913677603 1102 2 -0.10080579564597869 1102 3 -0.055901880936360726 1102 4 -0.09777441970929601 1102 5 0.05571720435080607 1102 6 0.13432800537870548 1102 7 0.5105133146118206 1102 8 0.032499889977998464 1102 9 -0.12126173364959669 1102 10 -0.11755519632912836 1102 11 0.3126090035376521 1102 12 0.5471476616926907 1102 13 0.2598703506718678 1102 14 0.3043297098141622 1102 15 0.39755019940877645 1102 16 0.49483699341934795 1102 17 0.14572563327887877 1102 18 0.3661827673755056 1102 19 0.07555599954520176 1102 20 0.2856726425296898 1102 21 0.44700122217477656 1102 22 0.5071887360077625 1102 23 0.2991565198254591 1102 24 -0.07804198241082361 1102 25 0.12616429344559776 1102 26 -0.01739580108287697 1102 27 0.08405967870492503 1102 28 -0.1959313793786066 1102 29 0.17615562763371884 1102 30 0.1218400205251271 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0.02515327720001892 1103 17 0.0630636932208759 1103 18 0.1635217480854152 1103 19 -0.3572969998563025 1103 20 -0.17056786205112928 1103 21 0.170471596466939 1103 22 0.25211300284630767 1103 23 -0.1188030584628379 1103 24 0.039424985040035665 1103 25 0.4858392623696801 1103 26 0.5475413565065494 1103 27 0.14911193228441277 1103 28 -0.04216313666418614 1103 29 0.23149377965249585 1103 30 0.18086124422569583 1103 31 0.08734988011864431 1103 32 -0.06823039148393661 1103 33 0.004341188518743877 1103 34 0.4574029129087336 1103 35 -0.0195208174832473 1103 36 -0.20922887227088438 1103 37 0.04070944617436112 1103 38 -0.01646929735184699 1103 39 0.11520145583354235 1103 40 0.06691631832661216 1103 41 0.6384339292174062 1103 42 0.35361687613544035 1103 43 0.21260259313627472 1103 44 -0.4213360086480975 1103 45 -0.17280338616343727 1103 46 -0.06433674224383168 1103 47 -0.017488828698766667 1103 48 0.08427186085392435 1103 49 0.30061628720213207 1103 50 0.20205328430868263 1104 1 0.400095607464209 1104 2 0.4191942043540209 1104 3 0.8610931929552628 1104 4 0.8265112945764201 1104 5 0.9395103077990825 1104 6 0.6754117492210696 1104 7 0.627360605252214 1104 8 0.7618841264311982 1104 9 0.7908072318676389 1104 10 0.8523709120452594 1104 11 1.2103676242273096 1104 12 1.1788225385399755 1104 13 0.46592946397235835 1104 14 0.8654873452821406 1104 15 0.9108506920196149 1104 16 0.8191719687888082 1104 17 0.6334842539685397 1104 18 1.076232028459252 1104 19 1.1455067384614566 1104 20 1.0683573715937342 1104 21 1.1704248674949724 1104 22 0.6475335635939672 1104 23 1.1952156331259474 1104 24 1.2854062934512456 1104 25 0.597568234332293 1104 26 1.2100179920205676 1104 27 1.1328035210694622 1104 28 0.8610144334292755 1104 29 1.158643312362777 1104 30 1.3318892816088734 1104 31 0.8022564221747146 1104 32 0.8710686739366267 1104 33 0.8126589455280921 1104 34 0.7529636605722843 1104 35 1.055806285320486 1104 36 0.9172197258599581 1104 37 0.9504758013145462 1104 38 1.3961007961060972 1104 39 1.3256220165812067 1104 40 1.378364916457457 1104 41 1.225863835759462 1104 42 1.4634766892195543 1104 43 1.3290511912671192 1104 44 1.3876826829406923 1104 45 1.0976639035561888 1104 46 0.9974389586907435 1104 47 0.9583990217685283 1104 48 1.1072157860875649 1104 49 0.9163172217815756 1104 50 0.9754177702899938 1105 1 0.4166797658800512 1105 2 0.46850113504709157 1105 3 0.4299050741433845 1105 4 0.31453109655661393 1105 5 0.5982231790862116 1105 6 0.47461967001314775 1105 7 0.4071625854502301 1105 8 0.5900524432628842 1105 9 0.8051636675112093 1105 10 0.6150936843224812 1105 11 0.6815062380886943 1105 12 0.734169073193442 1105 13 0.7420680778337427 1105 14 0.9367249690445192 1105 15 0.783127919742391 1105 16 0.9550135529472215 1105 17 0.8166030658497263 1105 18 0.6566280680632133 1105 19 0.6761998077683913 1105 20 0.6495949953561109 1105 21 0.48443476848507316 1105 22 0.32743455369298147 1105 23 0.14274038560120061 1105 24 0.35708946176807643 1105 25 -0.046639686459784 1105 26 0.7738298732086832 1105 27 0.5885956002773796 1105 28 0.722202552241157 1105 29 0.2842373717687181 1105 30 0.49149324200490696 1105 31 0.42814751128362616 1105 32 0.9233954066098917 1105 33 0.5000846881023477 1105 34 0.3631121754237725 1105 35 0.35699440413237227 1105 36 0.2311801218995626 1105 37 0.3877530290373201 1105 38 0.3195166376902563 1105 39 0.3786418185613982 1105 40 0.3538599659624069 1105 41 0.6638341327891624 1105 42 0.4588727288235148 1105 43 0.17281356750473975 1105 44 0.20654406907930678 1105 45 0.31132726989282133 1105 46 0.4308052953244115 1105 47 0.1607752593922882 1105 48 -0.06669510500154274 1105 49 0.2545350435637488 1105 50 0.1462098494979145 1106 1 -0.30119305232960514 1106 2 0.01259626620969101 1106 3 0.02758803831608958 1106 4 -0.18761241676378404 1106 5 -0.051108248901947206 1106 6 -0.2076810342840858 1106 7 -0.03531980711892402 1106 8 -0.14466226532137938 1106 9 -0.22826493308080842 1106 10 -0.24664970651144813 1106 11 0.21804803659844652 1106 12 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1107 34 0.7277045932147743 1107 35 0.6390187205536506 1107 36 0.6583078087615135 1107 37 0.5170302054596263 1107 38 0.4849349929972888 1107 39 0.29720232746203123 1107 40 -0.07072834779125259 1107 41 0.22511254042267126 1107 42 0.09174093792421445 1107 43 0.21485430007540884 1107 44 0.4968277606608993 1107 45 -0.029589982454176322 1107 46 -0.4164988651434527 1107 47 -0.389165745070855 1107 48 -0.49923499111450553 1107 49 0.25507369846550887 1107 50 -0.15022989810378817 1108 1 -0.39365439253579027 1108 2 -0.43413912897931084 1108 3 -0.6159901403780665 1108 4 -0.5605720387569164 1108 5 -0.49923969220091635 1108 6 -0.4587549174455958 1108 7 -0.38347272808112365 1108 8 -0.4056158735687987 1108 9 -0.5352344347990226 1108 10 0.14257924537859168 1108 11 -0.07963237577268956 1108 12 -0.23117746146002105 1108 13 -0.07115386936097678 1108 14 -0.5905543213845235 1108 15 -0.312065974647048 1108 16 -0.3931196978778573 1108 17 -0.006932412698127166 1108 18 -0.46522630487408634 1108 19 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0.6164202558062613 2210 12 1.0161909595926133 2210 13 1.034876832393408 2210 14 0.8499610294926683 2210 15 0.8476927972827752 2210 16 0.823382495104596 2210 17 0.9442737276527495 2210 18 1.001495186353985 2210 19 0.9981383174088236 2210 20 0.6944100031726776 2210 21 0.7433196043370813 2210 22 0.6806914583308128 2210 23 0.7320577383891091 2210 24 0.8254062934512447 2210 25 0.9904629711744022 2210 26 0.6655443078100447 2210 27 0.6151719421220889 2210 28 0.7673302229029559 2210 29 0.2752222597312013 2210 30 0.6308366500299201 2210 31 1.1277827379641892 2210 32 0.5665949897260987 2210 33 0.7952905244754618 2210 34 0.8832268184670227 2210 35 1.007385232688911 2210 36 0.911430252175748 2210 37 1.0049494855250742 2210 38 1.0926797434745197 2210 39 0.8543062271075215 2210 40 1.2981017585627157 2210 41 1.118758572601564 2210 42 1.2668977418511318 2210 43 1.0227354017934331 2210 44 0.8400511039933249 2210 45 1.0355586403982926 2210 46 0.7937547481644316 2210 47 1.1983990217685232 2210 48 1.284321049245463 2210 49 0.9447382744131545 2210 50 0.8464704018689417 2211 1 0.26441599581372444 2211 2 0.4574466315384882 2211 3 0.54895727062517 2211 4 0.665054983896809 2211 5 0.4646559388670468 2211 6 0.6866738851433993 2211 7 0.46988487709687377 2211 8 0.6544569419651793 2211 9 0.6825548046831784 2211 10 0.46504081495788085 2211 11 0.5477948086933324 2211 12 0.5261040919380378 2211 13 0.2054440270791531 2211 14 0.6867009375151483 2211 15 0.3215303036701016 2211 16 0.252764201798513 2211 17 0.7012997879491181 2211 18 0.14283397020682287 2211 19 0.386283437490583 2211 20 0.27821174052576936 2211 21 0.6912501102134243 2211 22 0.17675686456484385 2211 23 0.7350214583686707 2211 24 0.7147266818007623 2211 25 1.1927624090895748 2211 26 0.8928820696904722 2211 27 0.522172453108297 2211 28 0.5594610353710223 2211 29 0.5523811764404485 2211 30 0.5683941359778046 2211 31 0.21143117945626955 2211 32 0.46189391665507173 2211 33 0.4902317610620699 2211 34 0.6983520100868503 2211 35 0.36901016881563464 2211 36 0.2631420559570472 2211 37 0.7028544420912439 2211 38 0.724207592222605 2211 39 0.5835346379404243 2211 40 0.44705423684580836 2211 41 0.5725143211963513 2211 42 0.42080678630692603 2211 43 0.8694395407816842 2211 44 0.6946244305135068 2211 45 0.575722155983371 2211 46 0.7674389586907466 2211 47 0.45665144895298937 2211 48 0.6829924851166922 2211 49 0.3869968334320575 2211 50 0.895320682911354 2212 1 0.08494782421297842 2212 2 -0.02691417003021357 2212 3 0.4761178234971375 2212 4 0.24705316649760078 2212 5 -0.1905882143684039 2212 6 0.05058416301417168 2212 7 -0.2727379169152755 2212 8 -0.42070208046535384 2212 9 -0.21978390113728352 2212 10 0.19278963125707804 2212 11 0.06630358481844212 2212 12 0.2023200754857939 2212 13 0.29927921766694254 2212 14 0.12206369996194155 2212 15 0.18080143093587253 2212 16 0.1450832988380668 2212 17 -0.17939751943048066 2212 18 0.5139660186070358 2212 19 -0.4170548378932253 2212 20 -0.17738154466242406 2212 21 -0.213097299992711 2212 22 0.2559572089141682 2212 23 -0.14419323386912453 2212 24 -0.5706528198492435 2212 25 -0.0019145242883951141 2212 26 -0.26370121980209404 2212 27 -0.17209795676305362 2212 28 -0.28460133011752475 2212 29 -0.24748969256332742 2212 30 -0.3806230336620686 2212 31 -0.3398864349681361 2212 32 -0.043438715225939006 2212 33 -0.30660213821575155 2212 34 0.07993410392203515 2212 35 0.3649688468968435 2212 36 0.2725399229042935 2212 37 -0.24166705582830872 2212 38 0.13893330842137175 2212 39 -0.4819395597734797 2212 40 -0.14589616728638788 2212 41 0.049533786498379584 2212 42 -0.15915877876074802 2212 43 -0.24651531119593528 2212 44 0.004012732201772451 2212 45 0.17273779518180277 2212 46 0.15413846607990678 2212 47 0.13731527792616305 2212 48 0.15797933288559562 2212 49 0.024593083850540154 2212 50 0.2278808244771824 2213 1 0.6317966383920464 2213 2 0.29094678167360755 2213 3 0.4141859764604199 2213 4 0.5082638718960055 2213 5 0.6138402047062996 2213 6 0.544896285303544 2213 7 0.4182884403037619 2213 8 0.6233789717920288 2213 9 0.7614257885686726 2213 10 0.6914946233854593 2213 11 0.4448521603097859 2213 12 0.4853173839008065 2213 13 0.4951047217043154 2213 14 0.5862605411584312 2213 15 0.6242527538752896 2213 16 0.2711307316754059 2213 17 0.5460100271644137 2213 18 0.6189124408303854 2213 19 0.8692180786676431 2213 20 0.8094398458205347 2213 21 0.9008887850207472 2213 22 0.7762964501919072 2213 23 0.14263831353832046 2213 24 0.7161279429357847 2213 25 0.3366919456724986 2213 26 0.634399435319537 2213 27 0.6162571293168781 2213 28 0.4750350519859765 2213 29 0.21477733298133472 2213 30 0.8539511372789761 2213 31 0.6461224015561626 2213 32 0.54266661208095 2213 33 0.3509063682085056 2213 34 0.4140461347990865 2213 35 0.5376619554235802 2213 36 0.6684568392620207 2213 37 0.9969191002836197 2213 38 0.7538843012607401 2213 39 0.8094879959626482 2213 40 0.8930556381069366 2213 41 0.8990081656563689 2213 42 1.122188029425736 2213 43 1.2397728407516553 2213 44 0.9328888685077033 2213 45 1.3477669963396934 2213 46 1.1334183401340425 2213 47 1.1715948980571866 2213 48 1.263865270623647 2213 49 1.2825027887918807 2213 50 0.6584074610116417 2214 1 0.32222204424581946 2214 2 -0.014598899094256268 2214 3 0.25936905502423 2214 4 -0.3375116939293292 2214 5 -0.2891103818560907 2214 6 -0.0011399749168603535 2214 7 -0.0953980154374463 2214 8 -0.14484001149983072 2214 9 -0.17873299801741638 2214 10 -0.0149279385294534 2214 11 -0.15865536427843807 2214 12 0.027443228195153324 2214 13 -0.19004754752189257 2214 14 -0.24985748230406557 2214 15 0.36142540466329365 2214 16 0.263309899823291 2214 17 0.30664517350876963 2214 18 -0.0738829140694861 2214 19 -0.06483808912474842 2214 20 -0.07147021461316427 2214 21 -0.3329659371027276 2214 22 0.09914275899626546 2214 23 0.240043219332847 2214 24 -0.0665477295372554 2214 25 0.17049926881505684 2214 26 -0.2702693643012708 2214 27 -0.10231142145927663 2214 28 -0.07852579645578572 2214 29 -0.35750611292457535 2214 30 -0.7320187643681422 2214 31 -0.22987001460688816 2214 32 -0.4014025904311893 2214 33 -0.7975709395293791 2214 34 -0.1392777187380574 2214 35 -0.41499831238066065 2214 36 -0.6026653316113055 2214 37 -0.41325983086936446 2214 38 -0.3791290889513732 2214 39 -0.04926304089006095 2214 40 0.1619856061126299 2214 41 -0.2920097274589324 2214 42 -0.10497158664252026 2214 43 0.020315559083208257 2214 44 0.5179700392625293 2214 45 -0.21314069414496117 2214 46 -0.14635414475753095 2214 47 0.09954844705588073 2214 48 0.2031353263174509 2214 49 -0.009889674770150236 2214 50 -0.1653868274111545 2215 1 -0.11388077048854583 2215 2 0.1117926295508731 2215 3 0.2441640590969989 2215 4 -0.23388240621098078 2215 5 0.0836835361455428 2215 6 -0.53372210904665 2215 7 -0.2189386073462103 2215 8 -0.3647694168758875 2215 9 -0.36155497285676574 2215 10 -0.23861333992324774 2215 11 -0.22038040726875607 2215 12 0.18614537318564572 2215 13 -0.3802910084685891 2215 14 -0.10880399330053336 2215 15 -0.040330410342590994 2215 16 -0.05925322806158562 2215 17 0.12454724609452228 2215 18 -0.3510120660289431 2215 19 0.3124358723197247 2215 20 -0.3447528646267415 2215 21 0.23975557615639076 2215 22 -0.0722302159335868 2215 23 -0.20714657159845729 2215 24 -0.41553858843851543 2215 25 -0.18790420661258622 2215 26 -0.10482452766446926 2215 27 -0.00542482538723732 2215 28 -0.4227650941297796 2215 29 -0.010687396298635576 2215 30 -0.45362252941475134 2215 31 -0.4984916093213485 2215 32 -0.5171596725200729 2215 33 -0.5408056214010415 2215 34 -0.3897528748607897 2215 35 -0.5989968642858088 2215 36 -0.6011267308329522 2215 37 -0.2664139624649806 2215 38 -1.0475606212167392 2215 39 -0.390244125151078 2215 40 -0.5375799654323075 2215 41 -0.6533093925869977 2215 42 -0.6393185863709974 2215 43 -0.7540984150320966 2215 44 -0.30889212020891677 2215 45 -0.8223360964438129 2215 46 -0.3152382066635866 2215 47 -0.23939625382202223 2215 48 -0.8199889383218846 2215 49 -0.6205331719192131 2215 50 -0.4635586076627618 2216 1 -0.8214561166737226 2216 2 -1.1812655657609206 2216 3 -1.6371826691136988 2216 4 -1.6708450272626578 2216 5 -1.2086506117411489 2216 6 -1.639071009399622 2216 7 -1.5091911188857168 2216 8 -1.7310469080515603 2216 9 -2.1212617336495967 2216 10 -2.2735486281846278 2216 11 -1.606931226347399 2216 12 -1.6909475764025501 2216 13 -1.4544153636138475 2216 14 -2.1291678271316528 2216 15 -1.5466205723481963 2216 16 -1.2884142381077481 2216 17 -1.2565732172958306 2216 18 -2.430204753149944 2216 19 -1.1510449856764708 2216 20 -0.548481708866035 2216 21 -1.5398624888268628 2216 22 -1.6997078157163727 2216 23 -1.5645544818165789 2216 24 -1.3539040513763396 2216 25 -1.818006478311382 2216 26 -2.7760164907380513 2216 27 -1.6827711915742185 2216 28 -1.1302499343868178 2216 29 -2.047161285338369 2216 30 -1.800984281609523 2216 31 -1.770099899664359 2216 32 -1.5968048892817706 2216 33 -1.5791801349316756 2216 34 -1.949622546324271 2216 35 -1.7943086572082478 2216 36 -2.027952687933144 2216 37 -1.2925701756969517 2216 38 -1.315910698146773 2216 39 -0.9400676385912092 2216 40 -1.2690488766459893 2216 41 -1.4299407619416868 2216 42 -1.0245118165275784 2216 43 -0.962443061606443 2216 44 -1.7578920297029867 2216 45 -1.6614165562139291 2216 46 -1.5486529953322403 2216 47 -1.7395320127142355 2216 48 -1.7681290414986393 2216 49 -0.7800046172988857 2216 50 -1.8722833791352969 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 73.0 8 344.0 9 647.0 10 768.0 11 702.0 12 659.0 13 703.0 14 725.0 15 797.0 16 691.0 17 652.0 18 583.0 19 616.0 20 541.0 21 549.0 22 551.0 23 483.0 24 577.0 25 564.0 26 627.0 27 668.0 28 682.0 29 769.0 30 846.0 31 1061.0 32 1372.0 33 1838.0 34 2741.0 35 3892.0 36 6723.0 37 11659.0 38 20832.0 39 54110.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.90121299687511 22.831054064043546 12.569302106784047 24.6984308322973 2 21.730765999630357 20.180788682225245 33.72733840751382 24.361106910630575 3 20.359356887253664 23.890764914402837 28.152982880567173 27.596895317776323 4 15.981923106000151 19.78630288877502 34.2788982502709 29.95287575495393 5 16.317924853209238 34.25705813670231 33.296933144052346 16.128083866036103 6 30.94996093979689 34.56869975723873 17.86017287289894 16.621166430065436 7 30.679479533293573 27.195981419103376 21.88463380009576 20.239905247507288 8 27.337102152931198 32.38804841785177 19.808143002343613 20.466706426873422 9 26.564521277489373 16.90354988827848 21.72269542865783 34.80923340557431 10 22.831702297450544 24.24041328909236 29.189802175647866 23.738082237809234 11 34.79915326848445 23.355677637215866 21.17836802580515 20.666801068494532 12 24.267930043848594 24.14612838734607 25.996673554760346 25.58926801404499 13 30.624810994254226 19.553274419542355 23.80632371224085 26.01559087396257 14 25.550216726588488 20.301737172810054 23.456033063405126 30.692013037196332 15 26.55533341173704 26.281480485878934 21.559617613951378 25.603568488432654 16 27.345490621400526 24.132132738080227 23.35948075096056 25.16289588955869 17 26.114093226817204 25.56303184563561 24.681837655301024 23.641037272246162 18 25.773031156708576 25.043098011184462 24.40314510364546 24.780725728461505 19 26.047090690377452 24.4513236370366 25.864549071729602 23.637036600856348 20 25.89550920121505 24.922797304006124 25.4133604839999 23.768333010778928 21 25.523692452766646 24.742363520021556 24.522614757687 25.2113292695248 22 27.059861373660997 23.831127914303718 24.620037807183365 24.48897290485192 23 27.432810491560883 24.283157885893353 24.989708390392256 23.29432323215351 24 25.545849692709954 25.456731375532822 24.450366982504224 24.547051949253003 25 26.858160616320394 24.343647346444982 24.89897419956481 23.899217837669813 26 25.440475890404052 25.25395104983238 26.034498693485915 23.271074366277652 27 25.381674578834602 25.43376885266563 25.206066462210647 23.978490106289126 28 26.487145017644092 24.501764409342968 25.776340110905732 23.234750462107208 29 26.074864512876527 24.803596185354788 25.55392177456623 23.567617527202454 30 26.18989586572646 24.67957068775687 24.6400685823787 24.490464864137973 31 26.36120727321615 23.898430410378786 25.842772157429504 23.897590158975564 32 25.44933576452201 24.664521170667765 25.766118528850757 24.120024535959462 33 26.242332577094363 24.844971010839426 24.910511721704058 24.002184690362157 34 27.009108631352515 23.755545845657437 25.771376714170476 23.463968808819573 35 26.32251125899039 25.15628150836862 25.413389796329906 23.107817436311084 36 26.527354154588156 24.901481350776805 24.61747876282423 23.95368573181081 37 26.877058546750014 23.597667540498758 25.429354036432077 24.09591987631915 38 26.00073942123483 25.39407790811011 25.571370954189494 23.033811716465564 39 25.471595062641267 24.770819504079455 26.18329398122863 23.574291452050648 40 26.017600834609368 24.901983879923943 25.763516128489456 23.316899156977232 41 25.49533652634232 24.53407276699437 26.049071506596082 23.92151920006722 42 25.470754804177766 25.31026543764862 25.24976682827638 23.969212929897235 43 25.383981985615378 24.113564562747865 26.678765880217785 23.82368757141897 44 24.81683750630146 25.417576877835657 26.05864560578054 23.706940010082338 45 25.039489161485463 24.326163669971436 26.24936985380608 24.384977314737018 46 25.61287070486432 24.045198689406032 25.763252961438294 24.578677644291353 47 25.627389203948752 25.282923755513547 26.11468178954001 22.97500525099769 48 24.884060893235205 24.818530093759453 25.583056087643243 24.7143529253621 49 25.994908889280943 24.396165704732383 26.609034621233125 22.999890784753553 50 26.17393276929669 25.000210043436983 25.360644581299415 23.465212605966915 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.0 7 2.0 8 2.0 9 2.5 10 3.0 11 4.5 12 6.0 13 7.5 14 9.0 15 16.0 16 23.0 17 26.5 18 30.0 19 46.0 20 62.0 21 149.5 22 237.0 23 404.5 24 572.0 25 700.5 26 829.0 27 1031.5 28 1234.0 29 1568.5 30 1903.0 31 2285.5 32 2668.0 33 3460.0 34 4252.0 35 4926.5 36 5601.0 37 5971.5 38 6342.0 39 6896.0 40 7450.0 41 7804.5 42 8159.0 43 8411.5 44 8664.0 45 9216.5 46 9769.0 47 9019.5 48 8270.0 49 7715.5 50 7161.0 51 6877.5 52 6594.0 53 5903.5 54 5213.0 55 4544.0 56 3875.0 57 3500.5 58 3126.0 59 2882.5 60 2639.0 61 2510.5 62 2382.0 63 2273.0 64 2164.0 65 2143.5 66 2123.0 67 2190.5 68 2258.0 69 2340.0 70 2422.0 71 2440.0 72 2458.0 73 2480.5 74 2503.0 75 2345.0 76 2187.0 77 2047.0 78 1907.0 79 1655.0 80 1403.0 81 1217.0 82 1031.0 83 840.5 84 650.0 85 525.0 86 400.0 87 318.0 88 236.0 89 171.5 90 107.0 91 79.5 92 52.0 93 40.0 94 28.0 95 26.0 96 24.0 97 18.0 98 12.0 99 8.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002520013104068141 2 0.010920056784295277 3 8.400043680227137E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 8.400043680227137E-4 10 0.0016800087360454275 11 8.400043680227137E-4 12 8.400043680227137E-4 13 0.002520013104068141 14 0.002520013104068141 15 0.004200021840113569 16 0.08904046301040766 17 0.003360017472090855 18 0.1108805765789982 19 0.14280074256386133 20 0.1713608910766336 21 0.2310012012062463 22 0.018480096096499702 23 0.015120078624408846 24 0.08736045427436222 25 0.015120078624408846 26 0.023520122304635984 27 0.02688013977672684 28 0.02268011793661327 29 0.02688013977672684 30 0.0554402882894991 31 0.02940015288079498 32 0.03192016598486312 33 0.03108016161684041 34 0.03276017035288584 35 0.026040135408704125 36 0.02856014851277227 37 0.026040135408704125 38 0.02940015288079498 39 0.030240157248817692 40 0.1587608255562929 41 0.03108016161684041 42 0.030240157248817692 43 0.026040135408704125 44 0.02268011793661327 45 0.02268011793661327 46 0.014280074256386135 47 0.018480096096499702 48 0.01596008299243156 49 0.01344006988836342 50 0.020160104832545128 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 119047.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.28839029962956 #Duplication Level Percentage of deduplicated Percentage of total 1 67.76230938472042 30.010836056347497 2 12.180411197936424 10.789016102883735 3 6.190729079735983 8.225322771678414 4 3.374174948789925 5.977471082849631 5 2.3139367271071998 5.124026644938554 6 1.5780289811091723 4.193301805169387 7 1.2745618693574083 3.9513805471788457 8 0.9388513769820197 3.326417297369946 9 0.7586677793794098 3.024015724881769 >10 3.6150519687428875 25.01365007098037 >50 0.01327668613913967 0.36456189572185776 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.400043680227137E-4 0.0 4 0.0 0.0 0.0 8.400043680227137E-4 0.0 5 0.0 0.0 0.0 8.400043680227137E-4 0.0 6 0.0 0.0 0.0 8.400043680227137E-4 0.0 7 0.0 0.0 0.0 8.400043680227137E-4 0.0 8 0.0 0.0 0.0 8.400043680227137E-4 0.0 9 0.0 0.0 0.0 8.400043680227137E-4 0.0 10 0.0 0.0 0.0 8.400043680227137E-4 0.0 11 0.0 0.0 0.0 8.400043680227137E-4 0.0 12 0.0 0.0 0.0 8.400043680227137E-4 0.0 13 0.0 0.0 0.0 8.400043680227137E-4 0.0 14 0.0 0.0 0.0 8.400043680227137E-4 0.0 15 0.0 0.0 0.0 0.0016800087360454275 0.0 16 0.0 0.0 0.0 0.0016800087360454275 0.0 17 0.0 0.0 0.0 0.0016800087360454275 0.0 18 0.0 0.0 0.0 0.002520013104068141 0.0 19 0.0 0.0 0.0 0.002520013104068141 0.0 20 0.0 0.0 0.0 0.004200021840113569 0.0 21 0.0 0.0 0.0 0.009240048048249851 0.0 22 0.0 0.0 0.0 0.011760061152317992 0.0 23 0.0 0.0 0.0 0.014280074256386133 0.0 24 0.0 0.0 0.0 0.020160104832545128 0.0 25 0.0 0.0 0.0 0.021000109200567843 0.0 26 0.0 0.0 0.0 0.021840113568590558 0.0 27 0.0 0.0 0.0 0.02772014414474955 0.0 28 0.0 0.0 0.0 0.042000218401135686 0.0 29 0.0 0.0 0.0 0.04788024897729468 0.0 30 0.0 0.0 0.0 0.060480314497635385 0.0 31 0.0 0.0 0.0 0.0730803800179761 0.0 32 0.0 0.0 0.0 0.08232042806622594 0.0 33 0.0 0.0 0.0 0.10248053289877107 0.0 34 0.0 0.0 0.0 0.13692071198770234 0.0 35 0.0 0.0 0.0 0.18144094349290615 0.0 36 0.0 0.0 0.0 0.23436121867833712 0.0 37 0.0 0.0 0.0 0.3032415768561997 0.0 38 0.0 0.0 0.0 0.3864020092904483 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAACGG 25 4.4382297E-5 43.967224 2 TCAGATG 25 4.4382297E-5 43.967224 4 TGTATAA 30 1.2965691E-4 36.65476 11 TAGATGC 25 0.0023528917 35.17378 5 CTTGTAC 35 3.2146086E-4 31.40516 3 GTTCGAT 30 0.005733577 29.323807 43 TTCGATT 30 0.005733577 29.323807 44 CAAAGGT 30 0.005733577 29.323807 30 TTGTACT 30 0.005745374 29.311485 4 AAAAACG 30 0.005745374 29.311485 1 TTGGTGA 40 6.9735653E-4 27.514198 17 AGAGACA 40 6.9735653E-4 27.514198 17 CGTCAGA 40 7.0252194E-4 27.479519 2 GAGACAG 45 0.0013866422 24.457066 18 AGCTTTG 45 0.00139346 24.436504 44 AAAGGTA 45 0.00139346 24.436504 31 CAGATGT 45 0.0013968791 24.426237 5 GTGTATA 50 0.0025717586 21.992853 10 TAGCTAC 50 0.0025717586 21.992853 44 GCGTCAG 50 0.002578048 21.983612 1 >>END_MODULE