FastQCFastQC Report
Wed 25 May 2016
SRR1780189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119047
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG35622.9920955588969065Illumina PCR Primer Index 2 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT4580.38472200055440287No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC3270.27468142834342735No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT2840.2385612405184507Illumina PCR Primer Index 2 (95% over 21bp)
ACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTGC1300.10920056784295279Illumina PCR Primer Index 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTATC302.510542E-643.9949630
TCTTCTG4150.040.81459844
TCTTTAT452.2888344E-839.1724661
GTCTTCT4400.038.4955943
CGTCTTC4600.036.3436642
TTATCAA250.002345624835.19596532
CCGTCTT5050.033.10511841
GCCGTCT5100.032.78055640
TTATATA353.1869777E-431.4513992
CGGGATA353.1869777E-431.4513994
GATAATC353.2027447E-431.4249697
TTTGCAA353.2027447E-431.42496940
CTTTATA502.3246957E-630.822372
CTTAGGG502.3246957E-630.822373
CTATACA300.005680768329.3793471
TGCTAAT300.005680768329.3793471
ACATGTC300.005727697629.32997310
TTCGGCT300.005727697629.32997323
TTTTGAC300.005727697629.32997331
TCGACGC300.005727697629.32997343