Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780189_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 119047 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG | 3562 | 2.9920955588969065 | Illumina PCR Primer Index 2 (95% over 24bp) |
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT | 458 | 0.38472200055440287 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC | 327 | 0.27468142834342735 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT | 284 | 0.2385612405184507 | Illumina PCR Primer Index 2 (95% over 21bp) |
ACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTGC | 130 | 0.10920056784295279 | Illumina PCR Primer Index 2 (96% over 25bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AATTATC | 30 | 2.510542E-6 | 43.99496 | 30 |
TCTTCTG | 415 | 0.0 | 40.814598 | 44 |
TCTTTAT | 45 | 2.2888344E-8 | 39.172466 | 1 |
GTCTTCT | 440 | 0.0 | 38.49559 | 43 |
CGTCTTC | 460 | 0.0 | 36.34366 | 42 |
TTATCAA | 25 | 0.0023456248 | 35.195965 | 32 |
CCGTCTT | 505 | 0.0 | 33.105118 | 41 |
GCCGTCT | 510 | 0.0 | 32.780556 | 40 |
TTATATA | 35 | 3.1869777E-4 | 31.451399 | 2 |
CGGGATA | 35 | 3.1869777E-4 | 31.451399 | 4 |
GATAATC | 35 | 3.2027447E-4 | 31.424969 | 7 |
TTTGCAA | 35 | 3.2027447E-4 | 31.424969 | 40 |
CTTTATA | 50 | 2.3246957E-6 | 30.82237 | 2 |
CTTAGGG | 50 | 2.3246957E-6 | 30.82237 | 3 |
CTATACA | 30 | 0.0056807683 | 29.379347 | 1 |
TGCTAAT | 30 | 0.0056807683 | 29.379347 | 1 |
ACATGTC | 30 | 0.0057276976 | 29.329973 | 10 |
TTCGGCT | 30 | 0.0057276976 | 29.329973 | 23 |
TTTTGAC | 30 | 0.0057276976 | 29.329973 | 31 |
TCGACGC | 30 | 0.0057276976 | 29.329973 | 43 |