FastQCFastQC Report
Wed 25 May 2016
SRR1780188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98669
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAA150.002218864270.01
CGTGATA200.006933306352.50000425
ACCTTAG200.006933306352.5000041
TATGACG305.853163E-446.66666411
CATGGTT350.001250197640.014
GAGTAAT350.001250197640.01
GAGACAG350.001250197640.07
GTCCCGG350.001250197640.01
AGGCTGG459.140871E-538.8888855
GCATGGT400.00240876435.00000413
AGTGTGC501.6991956E-435.0000048
GTTGCAC400.00240876435.00000437
AGATCTC552.9737994E-431.81818440
GTAGATC552.9737994E-431.81818438
CTTCCGA450.004289578631.11110918
AAGAGAC450.004289578631.1111095
CGACGAT450.004289578631.11110922
TTCCGAC450.004289578631.11110919
TGCATGG450.004289578631.11110912
TAAGACC450.004289578631.11110960