##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98669 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.608539662913376 34.0 32.0 34.0 21.0 34.0 2 30.742563520457285 34.0 32.0 34.0 21.0 34.0 3 30.47308678510981 34.0 32.0 34.0 11.0 34.0 4 30.44270236852507 34.0 32.0 34.0 21.0 34.0 5 30.441668609188298 34.0 32.0 34.0 11.0 34.0 6 33.24847723195735 37.0 34.0 38.0 21.0 38.0 7 33.766086612816586 38.0 34.0 38.0 21.0 38.0 8 33.63879232585716 38.0 34.0 38.0 21.0 38.0 9 34.042708449462346 38.0 35.0 38.0 21.0 38.0 10-11 33.81082710881837 38.0 34.0 38.0 21.0 38.0 12-13 33.89426263568091 38.0 34.0 38.0 21.0 38.0 14-15 33.85677872482745 38.0 34.0 38.0 21.0 38.0 16-17 33.9015293557247 38.0 34.5 38.0 21.0 38.0 18-19 33.9080207562659 38.0 34.5 38.0 21.0 38.0 20-21 33.95571557429385 38.0 34.5 38.0 21.0 38.0 22-23 33.94389321874145 38.0 35.0 38.0 21.0 38.0 24-25 33.94623437959237 38.0 35.0 38.0 21.0 38.0 26-27 33.93015536794738 38.0 35.0 38.0 21.0 38.0 28-29 34.032963747478945 38.0 35.0 38.0 21.0 38.0 30-31 34.04193312995976 38.0 35.0 38.0 21.0 38.0 32-33 34.11750397794647 38.0 35.5 38.0 21.0 38.0 34-35 34.034524521379566 38.0 35.0 38.0 21.0 38.0 36-37 33.99447648197509 38.0 35.0 38.0 21.0 38.0 38-39 33.99627035847125 38.0 35.0 38.0 21.0 38.0 40-41 34.01148790400227 38.0 35.0 38.0 21.0 38.0 42-43 34.09920035674832 38.0 35.5 38.0 21.0 38.0 44-45 34.162335688007374 38.0 35.5 38.0 21.0 38.0 46-47 34.08980530865824 38.0 35.5 38.0 21.0 38.0 48-49 34.04625566287284 38.0 35.0 38.0 21.0 38.0 50-51 34.01084433814065 38.0 35.0 38.0 21.0 38.0 52-53 34.04530805014747 38.0 35.0 38.0 21.0 38.0 54-55 34.01499964527866 38.0 35.0 38.0 21.0 38.0 56-57 33.96587073954332 38.0 35.0 38.0 21.0 38.0 58-59 34.01279530551642 38.0 35.0 38.0 21.0 38.0 60-61 34.05110521034976 38.0 35.5 38.0 21.0 38.0 62-63 33.890223879840676 38.0 35.0 38.0 21.0 38.0 64-65 33.86485116906019 38.0 35.0 38.0 20.0 38.0 66-67 33.78200346613424 38.0 34.5 38.0 20.0 38.0 68-69 33.717464451854184 38.0 34.0 38.0 20.0 38.0 70-71 33.64413848321155 38.0 34.0 38.0 19.0 38.0 72-73 33.685554733502926 38.0 34.0 38.0 19.0 38.0 74-75 33.743693561300915 38.0 34.0 38.0 20.0 38.0 76 32.12436530217191 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.733634034788988 1101 2 -0.3067059362743976 1101 3 -0.2493782648540659 1101 4 -0.1753389168461652 1101 5 -0.7070209907501557 1101 6 -0.8233628239685444 1101 7 -0.7798727573847515 1101 8 -1.4807033212177458 1101 9 -1.2910579502379136 1101 10-11 -0.9078291597972026 1101 12-13 -0.3792070974897612 1101 14-15 -0.5881861287166643 1101 16-17 -0.8419016725689801 1101 18-19 -0.7702635690758228 1101 20-21 -0.7277556965711511 1101 22-23 -0.8028369012521637 1101 24-25 -0.8435350424508457 1101 26-27 -1.1545655351088513 1101 28-29 -0.8456959280304019 1101 30-31 -0.9190218431429003 1101 32-33 -1.0814358423836268 1101 34-35 -0.6882820183223828 1101 36-37 -0.9052629694213437 1101 38-39 -0.4807348772647515 1101 40-41 -0.28524375248429124 1101 42-43 -0.6544506484592532 1101 44-45 -0.6592689765484607 1101 46-47 -0.6834243607713901 1101 48-49 -0.6844257132982179 1101 50-51 -0.7978473170298841 1101 52-53 -0.4404554024570757 1101 54-55 -0.48044161491760207 1101 56-57 -0.720167903427587 1101 58-59 -0.6677171844083958 1101 60-61 -0.9237983992939576 1101 62-63 -0.9351981981907969 1101 64-65 -0.7243763124314953 1101 66-67 -1.2480020752130017 1101 68-69 -1.7473874465607082 1101 70-71 -0.9089257880250088 1101 72-73 -0.860424281477826 1101 74-75 -0.9946527748185687 1101 76 -1.2202117147660942 1102 1 0.2081468317829298 1102 2 0.0720639644988168 1102 3 -0.2688324523288017 1102 4 0.4429052514612408 1102 5 -0.8279968294838085 1102 6 0.45412012753941156 1102 7 -0.14101393063394596 1102 8 0.5490647149785204 1102 9 0.40115646307229724 1102 10-11 -0.06797415347328695 1102 12-13 0.4041825208587184 1102 14-15 0.3832584620543287 1102 16-17 0.248751972483376 1102 18-19 0.33237943615281296 1102 20-21 0.11047595429658941 1102 22-23 -0.09332625490895197 1102 24-25 0.04140230515584875 1102 26-27 -0.1721942626328783 1102 28-29 -0.36169478059843385 1102 30-31 -0.1414345796377603 1102 32-33 0.18015908805330838 1102 34-35 -0.06553939513643314 1102 36-37 0.057086919095205246 1102 38-39 0.23573859489046356 1102 40-41 0.31277040786932275 1102 42-43 -0.0198751330927891 1102 44-45 0.16827721410779617 1102 46-47 -0.26758458399906004 1102 48-49 0.012130686894757048 1102 50-51 -0.050314947713253844 1102 52-53 0.09476945509185697 1102 54-55 -0.09881617322148628 1102 56-57 0.3340026206098159 1102 58-59 0.00802897234645883 1102 60-61 0.3267394594415123 1102 62-63 -0.1981165870920023 1102 64-65 0.1367192243189237 1102 66-67 -0.11589745027982445 1102 68-69 0.22165796827506767 1102 70-71 0.6412743606195974 1102 72-73 0.07486968276923989 1102 74-75 -0.15917235287654563 1102 76 -0.5533320777718451 1103 1 -0.25711488110824376 1103 2 -0.023329685245698784 1103 3 -0.46836469932272706 1103 4 -0.18321011066601756 1103 5 -0.16315202688066321 1103 6 -1.2140421624166073 1103 7 -1.0767614456960999 1103 8 -0.22651552754124538 1103 9 -0.29367283250184784 1103 10-11 0.0672166338261988 1103 12-13 -0.28886092424385623 1103 14-15 -0.5134164241850883 1103 16-17 -0.4189146009716396 1103 18-19 -0.2093115926495841 1103 20-21 0.012063518167749976 1103 22-23 -0.3978487146796468 1103 24-25 -0.18749161151293947 1103 26-27 0.029810211526317687 1103 28-29 -0.028757596776578964 1103 30-31 -0.6465696941609806 1103 32-33 -0.7817597161259613 1103 34-35 -0.21776249298324757 1103 36-37 -0.25630759497551736 1103 38-39 -0.5770076745250918 1103 40-41 -0.10443858112322602 1103 42-43 -0.2996562551915858 1103 44-45 -0.4139545270010885 1103 46-47 0.15104164575918588 1103 48-49 -0.12241185574956859 1103 50-51 -0.6310722499986312 1103 52-53 0.029652643947123636 1103 54-55 0.0357454354421165 1103 56-57 -0.03227467557444896 1103 58-59 -0.6162624072706961 1103 60-61 -0.11390166723311523 1103 62-63 -0.09732089855448578 1103 64-65 -0.22682527577005374 1103 66-67 -0.05729486238548276 1103 68-69 -0.0019800824151232632 1103 70-71 -0.025998181770525264 1103 72-73 -0.1433027757303975 1103 74-75 -0.038541648959117936 1103 76 -0.7417679015509862 1104 1 -0.4340162171822399 1104 2 -1.02762053827756 1104 3 -0.7248395194666131 1104 4 -0.16650918569171225 1104 5 -0.6969392827902183 1104 6 -0.33891369376296154 1104 7 0.025833621107601346 1104 8 -0.5751023723511111 1104 9 -0.044906131576873065 1104 10-11 0.1350609298179748 1104 12-13 -0.37331621715238583 1104 14-15 -0.16161786303401016 1104 16-17 0.05851345658231821 1104 18-19 -0.3228136481480419 1104 20-21 -0.6142211683687293 1104 22-23 -0.17794892022950393 1104 24-25 -0.12495307091684538 1104 26-27 0.16242840268767367 1104 28-29 -0.2640736296645585 1104 30-31 0.19764921642483557 1104 32-33 -0.07465539957919987 1104 34-35 0.06413627371767916 1104 36-37 -0.6074638128583558 1104 38-39 -0.2041402541756625 1104 40-41 -0.33148123271624286 1104 42-43 -0.11583045868592734 1104 44-45 -0.2864236945242098 1104 46-47 -0.1503458157002271 1104 48-49 0.3801960579194983 1104 50-51 0.013005345068172858 1104 52-53 -0.17937870240435672 1104 54-55 -0.3679416149176049 1104 56-57 -0.2724082829742329 1104 58-59 7.725362031010263E-4 1104 60-61 -0.1700424688775115 1104 62-63 -0.20057115549180082 1104 64-65 0.19800771498020708 1104 66-67 0.2507986627943737 1104 68-69 -0.24228069906465777 1104 70-71 -0.12444371106138163 1104 72-73 0.3070440896344593 1104 74-75 -0.36088234782858564 1104 76 0.41298543863400994 1105 1 -0.34498025314627867 1105 2 -0.21495820061522153 1105 3 0.8069786623515718 1105 4 0.6234508542683308 1105 5 0.22201676116582547 1105 6 0.6605868057375375 1105 7 0.30403042430440763 1105 8 0.2510464787977398 1105 9 0.07212683545609622 1105 10-11 0.24024324750029535 1105 12-13 -0.09337116220732611 1105 14-15 -0.05007940149554457 1105 16-17 0.6395449251137819 1105 18-19 0.4878132249788294 1105 20-21 0.5315081023605401 1105 22-23 0.6591015293209495 1105 24-25 0.34587609991232426 1105 26-27 0.2641891419484068 1105 28-29 0.1581166800257492 1105 30-31 0.4077141514897775 1105 32-33 0.23732012489632126 1105 34-35 0.7603026073840127 1105 36-37 0.31084288044833386 1105 38-39 0.4531025030670932 1105 40-41 0.23896082522581708 1105 42-43 0.2366545562990865 1105 44-45 0.7672850966845814 1105 46-47 0.44455927920487426 1105 48-49 0.6797589949824285 1105 50-51 0.4086721782350011 1105 52-53 0.5275019169762558 1105 54-55 0.35172621725022424 1105 56-57 0.41720210663615376 1105 58-59 0.5291191895497462 1105 60-61 0.30785463401959134 1105 62-63 0.44764313022248814 1105 64-65 0.1309123103848009 1105 66-67 0.5548196418153566 1105 68-69 0.7090804397964803 1105 70-71 0.42414520002753164 1105 72-73 -0.05628008618972302 1105 74-75 0.6239702995240535 1105 76 -0.12175482110625069 1106 1 -0.07764975755491221 1106 2 0.8252262526127083 1106 3 0.11791410372179456 1106 4 0.48462953066439596 1106 5 0.08851620375222424 1106 6 0.032204318659402986 1106 7 0.1706058778363797 1106 8 0.09451460487456131 1106 9 0.4539551520670173 1106 10-11 0.020128217602660925 1106 12-13 0.6138473439242205 1106 14-15 0.6097589485601915 1106 16-17 0.31050621020550295 1106 18-19 0.3822846955372583 1106 20-21 0.4155407363931687 1106 22-23 0.5457312039941016 1106 24-25 0.6548605936794303 1106 26-27 0.6851250900582713 1106 28-29 0.38207026267498634 1106 30-31 -0.11534250200165985 1106 32-33 0.698351846797614 1106 34-35 0.349514120508573 1106 36-37 0.703198713021564 1106 38-39 0.47225726135228285 1106 40-41 0.4773438190435968 1106 42-43 0.7058827918316695 1106 44-45 0.6595649183122276 1106 46-47 0.6705651573846652 1106 48-49 0.4945076521223939 1106 50-51 0.8242113492089516 1106 52-53 0.6764960387964649 1106 54-55 0.6789786749374684 1106 56-57 0.5990202884543336 1106 58-59 0.46971663558197463 1106 60-61 0.26732813153656565 1106 62-63 0.11974457949784778 1106 64-65 0.11392386404853028 1106 66-67 0.24246532946104793 1106 68-69 0.7617306880989005 1106 70-71 0.6209755435970052 1106 72-73 0.1298960565081657 1106 74-75 0.37905036438673534 1106 76 0.3150040721743821 1107 1 -0.19966556783159106 1107 2 -0.9476854733424958 1107 3 -0.3348395194666125 1107 4 -0.9887169778995037 1107 5 -0.37882239967333575 1107 6 -1.1560565509058165 1107 7 0.05018427045825291 1107 8 -0.06685561910435922 1107 9 -0.3445165211872592 1107 10-11 -0.35912738187033 1107 12-13 0.03194352310735127 1107 14-15 -0.3125269539430988 1107 16-17 -0.43989563432677414 1107 18-19 0.2085824557480649 1107 20-21 -0.20870168784924914 1107 22-23 -0.21460476438534215 1107 24-25 -0.08482320078697825 1107 26-27 0.0927206104798799 1107 28-29 0.5088159807250534 1107 30-31 -0.3213767576011364 1107 32-33 -0.09351903594284039 1107 34-35 0.19261029969170806 1107 36-37 -0.03999628039082381 1107 38-39 0.02261299257758509 1107 40-41 0.162317468582458 1107 42-43 0.16868252832706077 1107 44-45 -0.0014561620566766464 1107 46-47 -0.019566594921002434 1107 48-49 0.0054233306467708076 1107 50-51 0.02706378662660569 1107 52-53 0.04253688201122685 1107 54-55 0.09410383962785573 1107 56-57 0.24083847027251437 1107 58-59 0.0022660426966041314 1107 60-61 0.297295193460144 1107 62-63 0.6303704029497652 1107 64-65 0.5934297929022847 1107 66-67 0.1512532082489244 1107 68-69 0.32320631392235555 1107 70-71 0.08890044478277304 1107 72-73 -0.19292344283307017 1107 74-75 -0.34805767250391995 1107 76 0.10915426980284337 1108 1 -0.2659489902914842 1108 2 -0.06522558029436709 1108 3 -0.5807218724077892 1108 4 -0.13552179073372628 1108 5 0.2206027340165022 1108 6 -0.2070859626705186 1108 7 -0.7355109167075256 1108 8 -0.4845561538637142 1108 9 -0.8339817618289729 1108 10-11 -0.43090545673664593 1108 12-13 -0.9398078137910417 1108 14-15 -0.7469119806810696 1108 16-17 -0.6745747787118006 1108 18-19 -0.4617317153749312 1108 20-21 -0.46926318517544985 1108 22-23 -0.6559951387168965 1108 24-25 -0.8346694574714704 1108 26-27 -0.7878342023543468 1108 28-29 -0.7432227893284207 1108 30-31 -0.5515104474407053 1108 32-33 -0.8312730466380245 1108 34-35 -0.6030380960302182 1108 36-37 -0.5321486868079361 1108 38-39 -0.7009364726630594 1108 40-41 -0.4087921570859834 1108 42-43 -0.18720650910609749 1108 44-45 -0.4769173920032088 1108 46-47 -0.5101147232632499 1108 48-49 -0.6488480597275625 1108 50-51 -0.47675305829317693 1108 52-53 -0.618927021732091 1108 54-55 -0.31867690903525414 1108 56-57 -0.9432591233103764 1108 58-59 -0.34166443858681816 1108 60-61 -0.6225109562724711 1108 62-63 -0.49720981095397576 1108 64-65 -1.154618335439963 1108 66-67 -0.7716278077938554 1108 68-69 -0.522742883938605 1108 70-71 -0.3469752236664192 1108 72-73 -0.46055044818066904 1108 74-75 -0.1864600789210229 1108 76 0.5009056067012381 1109 1 0.1547283715623493 1109 2 9.508902938684116E-4 1109 3 0.6819786623515718 1109 4 0.10946484028231396 1109 5 0.06875335790241976 1109 6 0.23576163091236424 1109 7 0.39260851288249654 1109 8 0.33875044150170197 1109 9 0.8215440848733451 1109 10-11 0.6866301938872468 1109 12-13 0.6317162503800873 1109 14-15 0.7137609248447774 1109 16-17 0.5630589111277686 1109 18-19 0.5386582133238207 1109 20-21 0.5841013424537778 1109 22-23 0.6532740234934451 1109 24-25 0.10388892042514897 1109 26-27 0.4940842468435065 1109 28-29 0.07813416254322902 1109 30-31 0.7164262727018951 1109 32-33 0.4325415701177704 1109 34-35 0.8901102996917061 1109 36-37 0.2882612953667518 1109 38-39 0.6187493562139537 1109 40-41 0.3368629231279172 1109 42-43 0.65186434650888 1109 44-45 0.14301353491301683 1109 46-47 0.5609637081093055 1109 48-49 0.5229990882225266 1109 50-51 0.5019122714750921 1109 52-53 0.5474611244354648 1109 54-55 0.46274020326421095 1109 56-57 0.7410657429997869 1109 58-59 0.7383266487572087 1109 60-61 0.31237095103590207 1109 62-63 0.6711279787073323 1109 64-65 0.4903237322962255 1109 66-67 0.8750410870367986 1109 68-69 0.5036608593768932 1109 70-71 0.5552640811464116 1109 72-73 0.16662201171238422 1109 74-75 0.39436656992032937 1109 76 -0.1482699726213994 1110 1 0.1774279955393432 1110 2 0.13142534284861185 1110 3 0.8331896776136816 1110 4 0.13667121055437903 1110 5 -0.15614157684652952 1110 6 0.41708213522557 1110 7 -0.5273207063168002 1110 8 0.1100123304643219 1110 9 -0.14151718475726938 1110 10-11 0.04899471501089181 1110 12-13 0.01513529483927556 1110 14-15 0.13801717346233744 1110 16-17 0.07612294563340782 1110 18-19 0.002590418588134469 1110 20-21 0.3796526585342974 1110 22-23 0.5487064499477654 1110 24-25 0.6189989624512435 1110 26-27 0.2259190179118562 1110 28-29 0.298895609125843 1110 30-31 -0.42672513185461725 1110 32-33 0.28583730115072825 1110 34-35 -0.016533694999729676 1110 36-37 0.16211387223027884 1110 38-39 0.29596402111109654 1110 40-41 0.18988216665810853 1110 42-43 0.1804808030450431 1110 44-45 0.3272233336301156 1110 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-0.41819816167494395 2116 18-19 -0.09648732923873382 2116 20-21 -0.556796746317751 2116 22-23 -0.3743256244381499 2116 24-25 -0.5213075463169687 2116 26-27 -0.5156762209045027 2116 28-29 -0.8049524085620092 2116 30-31 -0.8770840378727272 2116 32-33 -0.5744997979194508 2116 34-35 -0.34633349510270506 2116 36-37 -0.9306262313527185 2116 38-39 -0.4544069998780884 2116 40-41 -0.7285840780037276 2116 42-43 -0.8627999311222041 2116 44-45 -0.6716711752592417 2116 46-47 -0.6718336639515528 2116 48-49 -0.631785269881334 2116 50-51 -0.2527400609780699 2116 52-53 -0.6797565921494666 2116 54-55 -0.6007735651250741 2116 56-57 -1.2142577198444187 2116 58-59 -0.919129657039349 2116 60-61 -0.8989251007684445 2116 62-63 -0.6343070772463903 2116 64-65 -1.0489617574560697 2116 66-67 -0.6451556940521002 2116 68-69 -0.5424066623130273 2116 70-71 -0.41521875551899257 2116 72-73 -1.1839532429085224 2116 74-75 -0.9110527686940344 2116 76 -0.5534786988882168 2117 1 0.022953575139975868 2117 2 0.02793326914849459 2117 3 0.2815481457316267 2117 4 0.1395008835342182 2117 5 1.144077079701912 2117 6 0.37222538742017264 2117 7 0.28654790682188747 2117 8 0.19819844577349244 2117 9 0.11296045117957476 2117 10-11 -0.05462598018831244 2117 12-13 -0.30753585214294077 2117 14-15 0.11062883300123616 2117 16-17 -0.18801738042208882 2117 18-19 0.0733081265530231 2117 20-21 -0.4111846674247417 2117 22-23 -0.042494231746175615 2117 24-25 -0.1259285271786439 2117 26-27 0.1404098375523617 2117 28-29 -0.13820043897859335 2117 30-31 0.02865928565076814 2117 32-33 -0.3058778665395536 2117 34-35 -0.004137798025553252 2117 36-37 0.06199811288109913 2117 38-39 0.020906945320874115 2117 40-41 0.052321473388232675 2117 42-43 0.4315499692561744 2117 44-45 0.015251887602907743 2117 46-47 0.4325679665527673 2117 48-49 0.096220286994388 2117 50-51 0.45609061882525026 2117 52-53 0.04830380231558706 2117 54-55 0.05259803265949614 2117 56-57 -0.24238940317562196 2117 58-59 0.031845538091076264 2117 60-61 0.08806011136162795 2117 62-63 0.09191625797579661 2117 64-65 -0.3096779363728501 2117 66-67 0.2112612178604607 2117 68-69 -0.3401016556411207 2117 70-71 -0.13546479350316787 2117 72-73 -0.48240682288913916 2117 74-75 -0.15302363165489652 2117 76 0.4611446582689993 2118 1 0.28212793278543913 2118 2 0.018484826945453392 2118 3 -0.17574449684217797 2118 4 -0.9740398902812402 2118 5 -0.6331868587436773 2118 6 -0.5173235192316099 2118 7 0.2820230199440559 2118 8 0.027887285050312016 2118 9 0.038190183872387706 2118 10-11 -0.7592425608090352 2118 12-13 -0.24042998573674623 2118 14-15 -0.15075813452722997 2118 16-17 -0.31767432622311276 2118 18-19 -0.1233550185423482 2118 20-21 -0.0784260811030606 2118 22-23 0.3417424178441948 2118 24-25 -0.03843076968865944 2118 26-27 -0.33124483583851116 2118 28-29 -0.22865830969041667 2118 30-31 0.19566149826064816 2118 32-33 0.4175957316425212 2118 34-35 -0.3775007657134708 2118 36-37 0.24471782902917028 2118 38-39 -0.14027470795717534 2118 40-41 -0.3994595779004655 2118 42-43 -0.26540221046356294 2118 44-45 -0.10941173589460362 2118 46-47 -2.1653321800130243E-4 2118 48-49 -0.3670324488678389 2118 50-51 -0.11631753793118804 2118 52-53 -0.43722453304430786 2118 54-55 -0.15012487283615172 2118 56-57 -0.08876251697552817 2118 58-59 -0.29584995894880706 2118 60-61 -0.1342473816118357 2118 62-63 -0.22543379285443166 2118 64-65 -0.21853236258928632 2118 66-67 -0.45248188019204605 2118 68-69 -0.08298044049969633 2118 70-71 0.11583586230643306 2118 72-73 0.17675158801842628 2118 74-75 0.044167749133272594 2118 76 0.6567313985564383 2119 1 1.123487059357732 2119 2 0.7520976792846952 2119 3 0.7719494713590755 2119 4 0.6365707618836502 2119 5 1.0928706778913018 2119 6 1.0883471188189517 2119 7 1.2819607508585875 2119 8 1.1227023458664505 2119 9 0.6850414437835468 2119 10-11 1.1778033937760384 2119 12-13 1.2016182520481422 2119 14-15 1.2796665406357093 2119 16-17 1.2444079519951572 2119 18-19 1.1756299953644103 2119 20-21 0.7106477700323168 2119 22-23 1.0234436092593597 2119 24-25 1.3156104939586513 2119 26-27 1.183512937418989 2119 28-29 1.2127776154206273 2119 30-31 0.5532520997800177 2119 32-33 0.9788996462923549 2119 34-35 0.6907253956049644 2119 36-37 0.9740237362897446 2119 38-39 1.0556238349462248 2119 40-41 1.130232397690051 2119 42-43 1.0506341546823563 2119 44-45 0.9288941298532407 2119 46-47 0.9109254818096062 2119 48-49 0.9330630303965606 2119 50-51 0.7668552795373671 2119 52-53 0.5726036042797844 2119 54-55 0.6525859080181746 2119 56-57 1.0783780866194732 2119 58-59 0.8259691286015283 2119 60-61 0.5286796805327114 2119 62-63 0.9912211118738696 2119 64-65 0.6419952641283047 2119 66-67 0.5987344426108905 2119 68-69 0.11052074261293399 2119 70-71 0.6388754239320704 2119 72-73 0.5635152560826882 2119 74-75 0.2787146377546321 2119 76 0.8273694491189403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 10.0 13 151.0 14 620.0 15 1188.0 16 1639.0 17 1912.0 18 1965.0 19 1609.0 20 1243.0 21 962.0 22 706.0 23 613.0 24 610.0 25 610.0 26 671.0 27 788.0 28 955.0 29 1159.0 30 1521.0 31 1934.0 32 2451.0 33 3467.0 34 4675.0 35 7348.0 36 14013.0 37 45849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.320282966281205 17.475600239183535 9.676798183826733 30.52731861070853 2 22.736624471718574 21.16064822791353 28.132442813852375 27.970284486515524 3 19.94547426243298 20.0721604556649 26.310188610404484 33.67217667149763 4 16.10637586273298 16.949599164884617 30.55569631799248 36.38832865438993 5 16.271574658707397 30.016519879597443 32.355653751431554 21.356251710263606 6 30.119895813274688 30.328674659720882 19.328259129007083 20.223170397997343 7 31.477971804720834 25.082852770373677 19.363731263112022 24.07544416179347 8 26.348701213146985 28.656416909059583 20.572824291317435 24.422057586475994 9 24.636917370197324 16.883722344404017 19.54007844409085 38.9392818413078 10-11 27.15037144391856 21.83107156249683 24.451955528078727 26.566601465505883 12-13 25.78165381224093 19.9054414253717 24.280675794829175 30.0322289675582 14-15 25.00646102859575 20.64640690797975 22.306511196582495 32.040620866842 16-17 25.954960524582187 23.02800271615198 22.983915920907275 28.033120838358556 18-19 25.012921991709657 22.385957088852628 23.746060059390487 28.855060860047228 20-21 24.75093494410605 22.70976699875341 24.131692831588442 28.407605225552096 22-23 26.35832936383261 22.44372599296638 23.23982203123575 27.95812261196526 24-25 25.192309641326048 23.580861263416068 22.53544679686629 28.691382298391595 26-27 24.254325066636937 23.772410787582725 24.020208981544357 27.95305516423598 28-29 25.193323130871903 23.034083653427114 24.89485045961751 26.87774275608347 30-31 25.053968318316794 22.482745340481813 24.631343177695122 27.831943163506267 32-33 24.361754958497603 23.463296476096847 24.579148466083574 27.595800099321977 34-35 24.71799653386575 23.23931528646282 24.535061670838864 27.507626508832562 36-37 24.748401220241416 22.15032076944126 24.896877438709218 28.2044005716081 38-39 24.0668295006537 22.570918930971224 26.19566429172283 27.166587276652244 40-41 24.245710405497167 21.708439327448335 26.372011472701658 27.673838794352832 42-43 23.00063849841389 21.862996483191278 26.32741793268402 28.808947085710813 44-45 22.24204157334117 21.311151425473046 27.99663521470776 28.450171786478023 46-47 22.530379349137014 21.899988851614996 27.910995348082984 27.65863645116501 48-49 21.89492140388572 22.23190667788262 27.876029958750976 27.997141959480686 50-51 21.79053198066262 23.4257973629002 26.711023725790266 28.07264693064691 52-53 21.14747286381741 23.32191468445003 26.794636613323338 28.735975838409228 54-55 21.103386068572703 22.884593945413453 26.670484143956052 29.341535842057787 56-57 21.029401331725264 23.066008574121557 26.60460732347546 29.29998277067772 58-59 21.494593033272864 22.995064305911683 25.957494248446828 29.552848412368625 60-61 21.69779768721686 22.08799116237116 26.36238332201603 29.851827828395948 62-63 21.58175313421642 22.90080977814714 25.83992946112761 29.67750762650883 64-65 22.534940052093365 22.610445023259583 24.644011797018315 30.210603127628737 66-67 22.360113105433317 23.233741093960617 23.41718270176043 30.988963098845634 68-69 22.38747732317141 23.2352613282794 23.42174340471678 30.95551794383241 70-71 23.70197326414578 23.08070417253646 23.41768944653336 29.799633116784403 72-73 23.612279439337584 23.20282966281203 22.681389291469458 30.50350160638093 74-75 23.13239213937508 24.215305719121506 22.5612907802856 30.091011361217806 76 23.027495971379057 23.154182164610972 21.934954240947004 31.88336762306297 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.5 15 4.5 16 7.5 17 11.0 18 8.5 19 10.0 20 16.5 21 19.0 22 28.0 23 48.0 24 74.5 25 90.0 26 129.0 27 199.5 28 298.5 29 366.0 30 421.5 31 561.0 32 814.5 33 984.0 34 1119.5 35 1449.5 36 1805.5 37 1967.0 38 2254.0 39 2934.0 40 3619.5 41 4100.0 42 4288.0 43 4459.0 44 4930.0 45 5229.0 46 5228.0 47 5189.0 48 5029.5 49 4689.0 50 4469.0 51 4205.0 52 3633.5 53 3041.5 54 2757.0 55 2591.5 56 2239.0 57 1945.0 58 1838.0 59 1807.5 60 1803.5 61 1888.5 62 1947.0 63 2069.5 64 2196.5 65 2222.5 66 2305.5 67 2367.0 68 2339.0 69 2357.5 70 2355.5 71 2307.0 72 2283.0 73 2077.5 74 1758.5 75 1621.0 76 1452.5 77 1080.0 78 714.0 79 552.0 80 421.5 81 220.0 82 103.0 83 57.0 84 41.0 85 17.5 86 10.0 87 10.0 88 7.0 89 3.0 90 2.0 91 3.5 92 5.0 93 3.0 94 3.0 95 4.0 96 3.0 97 2.5 98 3.5 99 14.5 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.067447729276673E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 98669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.09908887289828 #Duplication Level Percentage of deduplicated Percentage of total 1 77.57907138325831 52.05485005422169 2 12.95501918255143 17.385399669602407 3 4.251880494215026 8.558919214748299 4 1.8714315923028124 5.0228541892590375 5 1.0905356010029301 3.6586972605377577 6 0.6449566504546416 2.5965602164813673 7 0.43953720206627794 2.0644782049073163 8 0.3247439809080748 1.7432020188711754 9 0.23109687943690904 1.3955751046427955 >10 0.6117270338035827 5.519464066728152 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002026979091710669 0.0 2 0.0 0.0 0.0 0.002026979091710669 0.0 3 0.0 0.0 0.0 0.002026979091710669 0.0 4 0.0 0.0 0.0 0.013175364096119349 0.0 5 0.0 0.0 0.0 0.015202343187830017 0.0 6 0.0 0.0 0.0 0.015202343187830017 0.0 7 0.0 0.0 0.0 0.01621583273368535 0.0 8 0.0 0.0 0.0 0.01621583273368535 0.0 9 0.0 0.0 0.0 0.01621583273368535 0.0 10 0.0 0.0 0.0 0.01621583273368535 0.0 11 0.0 0.0 0.0 0.01621583273368535 0.0 12 0.0 0.0 0.0 0.01621583273368535 0.0 13 0.0 0.0 0.0 0.01621583273368535 0.0 14 0.0 0.0 0.0 0.017229322279540688 0.0 15 0.0 0.0 0.0 0.01824281182539602 0.0 16 0.0 0.0 0.0 0.01824281182539602 0.0 17 0.0 0.0 0.0 0.021283280462962025 0.0 18 0.0 0.0 0.0 0.02432374910052803 0.0 19 0.0 0.0 0.0 0.030404686375660035 0.0 20 0.0 0.0 0.0 0.033445155013226036 0.0 21 0.0 0.0 0.0 0.03851260274250271 0.0 22 0.0 0.0 0.0 0.04155307138006872 0.0 23 0.0 0.0 0.0 0.04358005047177938 0.0 24 0.0 0.0 0.0 0.05067447729276672 0.0 25 0.0 0.0 0.0 0.05371494593033273 0.0 26 0.0 0.0 0.0 0.057768904113754065 0.0 27 0.0 0.0 0.0 0.1439155155114575 0.0 28 0.0 0.0 0.0 0.2168867628130416 0.0 29 0.0 0.0 0.0 0.30708733239416636 0.0 30 0.0 0.0 0.0 0.38613951697088245 0.0 31 0.0 0.0 0.0 0.49762336701496923 0.0 32 0.0 0.0 0.0 0.6263365393385967 0.0 33 0.0 0.0 0.0 0.7743060130334756 0.0 34 0.0 0.0 0.0 0.9699094953835551 0.0 35 0.0 0.0 0.0 1.2384842250352188 0.0 36 0.0 0.0 0.0 1.6975949893076854 0.0 37 0.0 0.0 0.0 2.293526842270622 0.0 38 0.0 0.0 0.0 2.9715513484478406 0.0 39 0.0 0.0 0.0 3.68200752009243 0.0 40 0.0 0.0 0.0 4.454286554034195 0.0 41 0.0 0.0 0.0 5.414061153959197 0.0 42 0.0 0.0 0.0 6.589709027151385 0.0 43 0.0 0.0 0.0 7.732925234876202 0.0 44 0.0 0.0 0.0 8.664322127517254 0.0 45 0.0 0.0 0.0 9.601799957433439 0.0 46 0.0 0.0 0.0 10.563601536450152 0.0 47 0.0 0.0 0.0 11.383514579047118 0.0 48 0.0 0.0 0.0 12.202414132098227 0.0 49 0.0 0.0 0.0 12.928072646930646 0.0 50 0.0 0.0 0.0 13.629407412662538 0.0 51 0.0 0.0 0.0 14.382430145233052 0.0 52 0.0 0.0 0.0 15.341191255612198 0.0 53 0.0 0.0 0.0 16.37495059238464 0.0 54 0.0 0.0 0.0 17.416817845523923 0.0 55 0.0 0.0 0.0 18.19517781674082 0.0 56 0.0 0.0 0.0 18.954281486586467 0.0 57 0.0 0.0 0.0 19.685007449148163 0.0 58 0.0 0.0 0.0 20.335667737587286 0.0 59 0.0 0.0 0.0 20.92146469509167 0.0 60 0.0 0.0 0.0 21.55084170306783 0.0 61 0.0 0.0 0.0 22.197448033323536 0.0 62 0.0 0.0 0.0 22.85722972767536 0.0 63 0.0 0.0 0.0 23.565658920228238 0.0 64 0.0 0.0 0.0 24.334897485532437 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTAAA 15 0.0022188642 70.0 1 CGTGATA 20 0.0069333063 52.500004 25 ACCTTAG 20 0.0069333063 52.500004 1 TATGACG 30 5.853163E-4 46.666664 11 CATGGTT 35 0.0012501976 40.0 14 GAGTAAT 35 0.0012501976 40.0 1 GAGACAG 35 0.0012501976 40.0 7 GTCCCGG 35 0.0012501976 40.0 1 AGGCTGG 45 9.140871E-5 38.888885 5 GCATGGT 40 0.002408764 35.000004 13 AGTGTGC 50 1.6991956E-4 35.000004 8 GTTGCAC 40 0.002408764 35.000004 37 AGATCTC 55 2.9737994E-4 31.818184 40 GTAGATC 55 2.9737994E-4 31.818184 38 CTTCCGA 45 0.0042895786 31.111109 18 AAGAGAC 45 0.0042895786 31.111109 5 CGACGAT 45 0.0042895786 31.111109 22 TTCCGAC 45 0.0042895786 31.111109 19 TGCATGG 45 0.0042895786 31.111109 12 TAAGACC 45 0.0042895786 31.111109 60 >>END_MODULE