FastQCFastQC Report
Wed 25 May 2016
SRR1780188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98669
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT48734.938734556953045TruSeq Adapter, Index 5 (95% over 23bp)
CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG16751.6975949893076852TruSeq Adapter, Index 5 (96% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG13471.3651704182671356TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC12101.2263223504849547RNA PCR Primer, Index 5 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC3340.3385055083156817RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT2010.20371139871692223TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT1530.1550639005158662No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT1210.12263223504849548No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1000.10134895458553345No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG207.892799E-570.0000118
AGTCTTG207.892799E-570.0000111
TTAACTC207.892799E-570.0000168
TAACTCC207.892799E-570.0000169
GTAAACC207.892799E-570.0000117
CGATTAC150.002218864270.069
GTATCAG150.002218864270.01
AAAAGTC252.3803007E-456.00000460
ACCGAAG252.3803007E-456.00000421
TTTCCAA252.3803007E-456.00000439
CTTATAT252.3803007E-456.0000041
TTCCAAG252.3803007E-456.00000440
TCAGAGA252.3803007E-456.00000453
TCCAAGG252.3803007E-456.00000441
GTCTTTA252.3803007E-456.00000464
GGACAAA252.3803007E-456.00000446
TGTAAAC252.3803007E-456.00000416
TTAATAC200.006933306352.5000043
GTCCTTC200.006933306352.5000047
TCCCTAT200.006933306352.50000454
Produced by FastQC (version 0.11.5)