##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780188_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98669 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.66826460185063 34.0 34.0 34.0 34.0 34.0 2 33.681622394065 34.0 34.0 34.0 34.0 34.0 3 33.69602408051161 34.0 34.0 34.0 34.0 34.0 4 33.725060556000365 34.0 34.0 34.0 34.0 34.0 5 33.702145557368574 34.0 34.0 34.0 34.0 34.0 6 37.424277128581416 38.0 38.0 38.0 37.0 38.0 7 37.42335485309469 38.0 38.0 38.0 37.0 38.0 8 37.29757066555859 38.0 38.0 38.0 36.0 38.0 9 37.33211038928133 38.0 38.0 38.0 37.0 38.0 10-11 37.27879577172162 38.0 38.0 38.0 36.0 38.0 12-13 37.25946345863443 38.0 38.0 38.0 36.0 38.0 14-15 37.15713648663714 38.0 38.0 38.0 36.0 38.0 16-17 37.198203083035196 38.0 38.0 38.0 36.0 38.0 18-19 37.15284942585817 38.0 38.0 38.0 36.0 38.0 20-21 37.193743729033436 38.0 38.0 38.0 36.0 38.0 22-23 37.16785920603229 38.0 38.0 38.0 36.0 38.0 24-25 37.16586769907468 38.0 38.0 38.0 36.0 38.0 26-27 37.04193819740749 38.0 38.0 38.0 35.5 38.0 28-29 37.013747985689534 38.0 38.0 38.0 36.0 38.0 30-31 37.016768184536176 38.0 38.0 38.0 36.0 38.0 32-33 36.90161550233609 38.0 38.0 38.0 35.0 38.0 34-35 36.949259645886755 38.0 37.5 38.0 35.0 38.0 36-37 36.95811754451753 38.0 38.0 38.0 35.5 38.0 38-39 36.38912931113116 38.0 37.0 38.0 34.0 38.0 40-41 36.625561219836015 38.0 37.0 38.0 34.0 38.0 42-43 36.875872867871365 38.0 37.0 38.0 35.0 38.0 44-45 36.95421560976598 38.0 37.5 38.0 35.0 38.0 46-47 36.89678622465009 38.0 37.5 38.0 35.5 38.0 48-49 36.92171806747813 38.0 38.0 38.0 35.0 38.0 50-51 36.763831598577056 38.0 37.0 38.0 34.5 38.0 52-53 36.77993088001297 38.0 37.0 38.0 34.5 38.0 54-55 36.75777599854057 38.0 37.0 38.0 34.5 38.0 56-57 36.79490518805299 38.0 37.0 38.0 35.0 38.0 58-59 36.66328330073275 38.0 37.0 38.0 34.0 38.0 60-61 36.68760704983328 38.0 37.0 38.0 34.0 38.0 62-63 36.646657004732994 38.0 37.0 38.0 34.0 38.0 64-65 36.556279074481346 38.0 37.0 38.0 34.0 38.0 66-67 36.221564017067166 38.0 37.0 38.0 34.0 38.0 68-69 36.13885313522991 38.0 37.0 38.0 34.0 38.0 70-71 35.57102534737354 38.0 37.0 38.0 29.5 38.0 72-73 34.705175891110684 38.0 36.5 38.0 24.0 38.0 74-75 34.45178830230366 38.0 36.0 38.0 23.0 38.0 76 33.61838064640363 38.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07404188167743797 1101 2 -0.0965420890573867 1101 3 -0.055008377935457986 1101 4 -0.13398968430417568 1101 5 -0.014414035193340169 1101 6 -0.28659598954890697 1101 7 0.03928959614008676 1101 8 -0.29063637251919516 1101 9 -0.020830196328297745 1101 10-11 0.04387800781624662 1101 12-13 0.09971105277971759 1101 14-15 0.04028118017631499 1101 16-17 -0.22145799702511937 1101 18-19 -0.0585822470658286 1101 20-21 -0.06193230248187831 1101 22-23 -0.08125106085525147 1101 24-25 -0.041542038460057995 1101 26-27 -0.12672945782278333 1101 28-29 -0.4310411555429212 1101 30-31 -0.1366186747718885 1101 32-33 -0.16907505177867677 1101 34-35 -0.17962937835203974 1101 36-37 -0.05149793305034933 1101 38-39 0.044674066946349456 1101 40-41 0.06391654622807152 1101 42-43 -0.2914343020799848 1101 44-45 -0.289553345924503 1101 46-47 -0.33550806925101995 1101 48-49 -0.1463884506322657 1101 50-51 -0.08607376008624357 1101 52-53 -0.15515326831452825 1101 54-55 -0.3548052170453033 1101 56-57 -0.28040177569377533 1101 58-59 0.23874214315707576 1101 60-61 0.05456520994469827 1101 62-63 0.052364892411141284 1101 64-65 -0.2463032249961472 1101 66-67 -0.4244842550064689 1101 68-69 -1.0367015403767468 1101 70-71 -0.7582459408950015 1101 72-73 -0.5529953187264169 1101 74-75 -0.6542661022460763 1101 76 -1.6203186839862944 1102 1 0.14286187746616008 1102 2 0.05785270577395352 1102 3 0.09122400311626677 1102 4 0.03901176302478149 1102 5 0.03744206640654113 1102 6 0.01628693327325692 1102 7 0.1361824025239784 1102 8 0.1117210786171583 1102 9 0.24345728753593932 1102 10-11 0.03855157646005836 1102 12-13 -0.1254789906401541 1102 14-15 0.006360222592185494 1102 16-17 -0.11219769947514635 1102 18-19 0.09804873620513632 1102 20-21 0.10934799689356112 1102 22-23 -0.06604106776591578 1102 24-25 0.15592709170827845 1102 26-27 0.13089509722134807 1102 28-29 0.19147328514919337 1102 30-31 0.40455196661650916 1102 32-33 0.343129451891798 1102 34-35 0.27420604339440047 1102 36-37 0.4021940596999656 1102 38-39 0.26805820891498655 1102 40-41 0.11550534833516934 1102 42-43 0.18778153248118912 1102 44-45 0.09347378823376573 1102 46-47 0.26253868860171536 1102 48-49 0.23430470258902147 1102 50-51 0.3863552751581736 1102 52-53 0.2711372666756233 1102 54-55 -0.07024208872850579 1102 56-57 0.08858887534347559 1102 58-59 0.2634640864895772 1102 60-61 0.1732592237399615 1102 62-63 0.3531811337891 1102 64-65 0.1347810982145745 1102 66-67 0.3485132806225977 1102 68-69 0.2231200861080751 1102 70-71 0.12024362138577516 1102 72-73 0.3087065315076245 1102 74-75 0.19744135475782087 1102 76 0.4162687490258463 1103 1 -0.039663480339470425 1103 2 0.19747952022345316 1103 3 -0.049406955014752896 1103 4 -0.10559763779378528 1103 5 0.16595974696174665 1103 6 0.1675440194770843 1103 7 0.08513631360006713 1103 8 0.25584747361423155 1103 9 -0.033254205460472974 1103 10-11 0.24050520790119378 1103 12-13 0.3516290755570637 1103 14-15 0.35392104319365814 1103 16-17 0.3351800873413353 1103 18-19 0.3490274585276367 1103 20-21 0.15248804316043163 1103 22-23 0.27415391405781264 1103 24-25 0.028807585899500054 1103 26-27 0.43992349287938026 1103 28-29 0.10573975183449846 1103 30-31 0.24169638535713034 1103 32-33 0.48925142357240503 1103 34-35 0.25353847850637834 1103 36-37 0.23020585521055636 1103 38-39 0.14615008170650157 1103 40-41 0.20890977673591493 1103 42-43 0.09850171958238718 1103 44-45 0.14128500922163312 1103 46-47 0.2672289293419894 1103 48-49 0.32772819700903 1103 50-51 8.212995118341837E-4 1103 52-53 0.2186421602872386 1103 54-55 0.12832414662243252 1103 56-57 0.1554406942417117 1103 58-59 0.3955089806096197 1103 60-61 0.3225906419975786 1103 62-63 0.36753784629895137 1103 64-65 0.5194126156154937 1103 66-67 -0.1391700709293815 1103 68-69 0.4106026089237389 1103 70-71 0.38932235133467685 1103 72-73 -0.4269367559825241 1103 74-75 -0.36552735154921834 1103 76 -0.28864011540204615 1104 1 0.08412891431623848 1104 2 -0.11968389190398199 1104 3 0.14601956091536294 1104 4 0.15072301996572435 1104 5 0.02757331325157253 1104 6 0.184964368911821 1104 7 0.07077351812216648 1104 8 0.05900780249398707 1104 9 -0.060669416149067956 1104 10-11 0.04742966833284612 1104 12-13 -0.0973303283483844 1104 14-15 0.16412145429335112 1104 16-17 -0.03283121790018839 1104 18-19 0.018631775074389623 1104 20-21 0.12861856731253596 1104 22-23 0.019117942834789403 1104 24-25 0.0666287575336213 1104 26-27 -0.00822655850807763 1104 28-29 -0.05424226255399134 1104 30-31 0.05753657035199211 1104 32-33 0.11160496930725117 1104 34-35 0.09832265116825312 1104 36-37 -0.24597605640300912 1104 38-39 -0.0645642040077874 1104 40-41 -0.11706658503181444 1104 42-43 0.2070962725114711 1104 44-45 -0.04697295583488881 1104 46-47 0.06426130344270575 1104 48-49 0.198886984264945 1104 50-51 -0.32974139319114926 1104 52-53 -0.09584910384431566 1104 54-55 -0.30873905995515827 1104 56-57 -0.37301511254143804 1104 58-59 0.33426797341537906 1104 60-61 0.07578951147343105 1104 62-63 0.0031622039556893355 1104 64-65 -0.15965984070516726 1104 66-67 -0.46486380165907804 1104 68-69 -0.256265325300312 1104 70-71 -0.4226766209160928 1104 72-73 -0.20916954118293063 1104 74-75 0.17885856828633706 1104 76 0.04848732550237145 1105 1 0.0010619812493004588 1105 2 0.21767874545864885 1105 3 0.16345212834792733 1105 4 0.11495878420148387 1105 5 0.14500588068413833 1105 6 0.22177755572500502 1105 7 0.007911380260026135 1105 8 0.20528652377270618 1105 9 0.3419030114233621 1105 10-11 0.24839120679438764 1105 12-13 0.24556427704622052 1105 14-15 0.2749855901574847 1105 16-17 0.2226882626192932 1105 18-19 0.2160093974520123 1105 20-21 0.26530687900084615 1105 22-23 0.15128577500261997 1105 24-25 0.007338048242907291 1105 26-27 0.04332189304037115 1105 28-29 0.33937661856489143 1105 30-31 0.20757403288945397 1105 32-33 0.20965691735919734 1105 34-35 0.17017579802139693 1105 36-37 0.12068228525532732 1105 38-39 0.3122339977904218 1105 40-41 0.2942471012818757 1105 42-43 0.25803283594803617 1105 44-45 0.22398109011915324 1105 46-47 0.24162144330284718 1105 48-49 0.22527309815105667 1105 50-51 -0.05417945512920852 1105 52-53 0.43986518186996904 1105 54-55 0.1265381628220581 1105 56-57 0.3604389334126097 1105 58-59 0.44939035103774927 1105 60-61 0.3508519490358637 1105 62-63 0.192360505653987 1105 64-65 -0.017514486059802437 1105 66-67 -0.20482384161911682 1105 68-69 -0.1397193712543512 1105 70-71 0.11680889356942004 1105 72-73 0.38914464463125853 1105 74-75 0.4131867401145186 1105 76 0.11549531751036568 1106 1 -0.16162677926554636 1106 2 -0.03148513414001286 1106 3 0.06755165201266777 1106 4 0.14523647752265845 1106 5 -0.01611198695546534 1106 6 0.13900163599256388 1106 7 -0.08869853156727459 1106 8 0.15481525591013678 1106 9 0.30252934161490685 1106 10-11 0.2660890886227065 1106 12-13 0.3089067316930212 1106 14-15 0.0682104812084603 1106 16-17 0.3480176433834643 1106 18-19 0.36899409391497073 1106 20-21 0.17670345344090066 1106 22-23 0.17419040660289653 1106 24-25 0.03753973061850502 1106 26-27 0.18985832762028565 1106 28-29 0.3187649838228239 1106 30-31 0.22562663246379344 1106 32-33 0.3524434786240178 1106 34-35 0.1610038105885394 1106 36-37 0.25857880902141517 1106 38-39 0.17513558895287673 1106 40-41 0.21895825968868365 1106 42-43 0.23289854994418846 1106 44-45 0.1257496114528891 1106 46-47 0.04741347735575374 1106 48-49 0.23923377515521338 1106 50-51 0.4176964950076112 1106 52-53 0.2152275007105473 1106 54-55 0.28911290691854674 1106 56-57 0.42525093300722006 1106 58-59 0.39423174153131413 1106 60-61 0.5548526584713542 1106 62-63 0.05784646896604073 1106 64-65 0.25905133941906655 1106 66-67 0.33617139502828763 1106 68-69 0.4838744262524912 1106 70-71 0.7239589898499545 1106 72-73 0.540545779728042 1106 74-75 0.4300231645596355 1106 76 1.4309407416514404 1107 1 -0.09418277399544905 1107 2 0.07642000419991035 1107 3 -0.17333108843529033 1107 4 -0.06979646055376065 1107 5 -0.04995915428089859 1107 6 0.0754838494313006 1107 7 0.09798131032995627 1107 8 0.20738442587060746 1107 9 0.07225266177301393 1107 10-11 -0.02825214984896718 1107 12-13 0.10549434697629323 1107 14-15 0.1565240516959463 1107 16-17 0.12450644443747905 1107 18-19 0.15999541143802531 1107 20-21 0.21621596990993908 1107 22-23 -0.17906387534703327 1107 24-25 0.21831706922192495 1107 26-27 0.2980072077256821 1107 28-29 0.2728031919914642 1107 30-31 0.04659501191043347 1107 32-33 0.029656917359190516 1107 34-35 -0.1748591670135724 1107 36-37 -0.10980722523417796 1107 38-39 0.409786445342867 1107 40-41 0.05494640198117651 1107 42-43 0.18628458419978955 1107 44-45 -0.05210282596475935 1107 46-47 -0.11823869655729169 1107 48-49 0.10359477647273252 1107 50-51 0.14749886654910682 1107 52-53 0.1116833636881509 1107 54-55 0.30947522575912956 1107 56-57 -0.033826800853120176 1107 58-59 -0.08368657203916996 1107 60-61 -0.2550221768382599 1107 62-63 -0.0014157181222387294 1107 64-65 0.529268730723409 1107 66-67 0.15461671782144037 1107 68-69 0.15545545392047444 1107 70-71 0.2394662362267681 1107 72-73 0.05774604323265464 1107 74-75 0.05304687997465152 1107 76 -0.8053438433287923 1108 1 -0.15725764030560896 1108 2 -0.09510405997121296 1108 3 -0.005871195387157968 1108 4 -0.05526437499226233 1108 5 -0.21181744305094696 1108 6 0.03286352857568886 1108 7 0.019197887870063823 1108 8 0.14314645795617054 1108 9 0.029351592254293735 1108 10-11 -0.3727909199024424 1108 12-13 -0.45489335355846805 1108 14-15 -0.09853610873185659 1108 16-17 0.010288529998980778 1108 18-19 0.040322951544979446 1108 20-21 0.005614365631863905 1108 22-23 -0.0981614689299235 1108 24-25 -0.03836073826469999 1108 26-27 -0.049245466071106136 1108 28-29 0.21377244332836653 1108 30-31 0.09167522581417842 1108 32-33 -0.14779629119695414 1108 34-35 0.06192559234472128 1108 36-37 0.07760587637010019 1108 38-39 -0.059732271234672396 1108 40-41 -0.04175145898139476 1108 42-43 -0.06406969387508354 1108 44-45 -0.040197745750859326 1108 46-47 -0.028753402439647857 1108 48-49 -4.827636342170649E-4 1108 50-51 0.26064726227103563 1108 52-53 -0.18513481813003096 1108 54-55 0.020357578700298973 1108 56-57 0.15140715636613322 1108 58-59 0.12507679694478924 1108 60-61 -0.3288323372660713 1108 62-63 -0.07152266999388956 1108 64-65 0.1900775542528237 1108 66-67 0.2030458622064657 1108 68-69 0.0031359352038933253 1108 70-71 -0.055239646126175046 1108 72-73 -0.24283550756948813 1108 74-75 -0.3665721039825698 1108 76 0.431260434746072 1109 1 0.0829384381257583 1109 2 -0.016004238224333278 1109 3 0.12091133580713631 1109 4 -0.14873585449315385 1109 5 0.11382872450698045 1109 6 0.015483849431298324 1109 7 0.2220722194208662 1109 8 0.03162685011303523 1109 9 0.11346478298513318 1109 10-11 -0.07605518015200374 1109 12-13 0.10337313485508304 1109 14-15 0.340690718362616 1109 16-17 0.28708220201323087 1109 18-19 0.3057529871956106 1109 20-21 0.2406856668796351 1109 22-23 9.361246529664413E-4 1109 24-25 -0.036076870172010445 1109 26-27 -0.04918976197128444 1109 28-29 0.05106076774904267 1109 30-31 0.12977683009225416 1109 32-33 0.20965691735919734 1109 34-35 0.034686287531890514 1109 36-37 0.12723822931127415 1109 38-39 -0.16634991829349843 1109 40-41 -0.04331117377639515 1109 42-43 0.22219367510887622 1109 44-45 0.2794880831261537 1109 46-47 -0.15960233292092596 1109 48-49 0.19147356435152574 1109 50-51 0.2087867453369867 1109 52-53 0.34706215156693787 1109 54-55 0.05500552878942955 1109 56-57 -0.10678134630767033 1109 58-59 0.15888918553659437 1109 60-61 0.1757353989193149 1109 62-63 0.02540246369594712 1109 64-65 0.1765414579961373 1109 66-67 0.20120762691234972 1109 68-69 0.020682726647741845 1109 70-71 0.5598450241055559 1109 72-73 -0.15922365373704395 1109 74-75 0.18562263755041641 1109 76 0.030413732428783646 1110 1 0.05769512912332431 1110 2 0.18876240632333463 1110 3 0.11322498323026764 1110 4 0.1917032076612415 1110 5 -0.0147103155284114 1110 6 -0.0870377165939118 1110 7 -0.07893972483925182 1110 8 0.059607385392837386 1110 9 0.1651126485016121 1110 10-11 0.011024558843800492 1110 12-13 0.17614464558279508 1110 14-15 -0.07933527146264652 1110 16-17 0.17491122214152455 1110 18-19 0.10458068018387934 1110 20-21 0.34543295730210843 1110 22-23 0.08006684794427343 1110 24-25 0.3091730745304915 1110 26-27 0.26643454680996115 1110 28-29 0.20460810240951588 1110 30-31 0.14545367149902688 1110 32-33 0.2300616950632417 1110 34-35 0.25142484094927653 1110 36-37 0.28189151398280643 1110 38-39 0.24651504521015255 1110 40-41 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66-67 0.17620762691235115 2115 68-69 0.10534181755684102 2115 70-71 0.15738290289343126 2115 72-73 0.33547331595992347 2115 74-75 0.4026680920958654 2115 76 0.22586827788332897 2116 1 -0.0821722119433943 2116 2 0.053937921966792146 2116 3 -0.4573295795707111 2116 4 -0.04435700374500584 2116 5 0.16322832214309102 2116 6 0.033778832456576424 2116 7 -0.2015886632649142 2116 8 0.01779559146849863 2116 9 -0.014537228834306859 2116 10-11 0.12071805007558112 2116 12-13 -0.053517346724198944 2116 14-15 0.1512732029596222 2116 16-17 -0.6096391609944334 2116 18-19 -0.28292424152311924 2116 20-21 -0.3448402352956492 2116 22-23 -0.12694014845151713 2116 24-25 -0.15755618615339984 2116 26-27 -0.017888680618334263 2116 28-29 0.0370959117198737 2116 30-31 -0.36225447884777395 2116 32-33 -0.24432271296898023 2116 34-35 -0.07626241107241327 2116 36-37 -0.38131986197502954 2116 38-39 -1.0239743996213022 2116 40-41 -0.7939634433602052 2116 42-43 -0.06029406536641346 2116 44-45 0.062140855664814865 2116 46-47 -0.08288599946185826 2116 48-49 -0.2173029979520109 2116 50-51 -0.17214655027472503 2116 52-53 -0.35370328286031594 2116 54-55 -0.3582426165645245 2116 56-57 -0.40098636328239934 2116 58-59 -0.2902652216053667 2116 60-61 -0.5303975069023892 2116 62-63 -0.1409027041652351 2116 64-65 -0.3826814767454678 2116 66-67 -0.2840102154112998 2116 68-69 -0.5038127467585198 2116 70-71 -0.6255960044371349 2116 72-73 -1.0712694226292854 2116 74-75 -1.059174923118519 2116 76 -0.719429094177535 2117 1 -0.030314131624606944 2117 2 -0.14152152563589482 2117 3 -0.306434812904655 2117 4 0.07224599038737267 2117 5 -0.43060793281513554 2117 6 -0.12047930635564086 2117 7 -0.08644800484825765 2117 8 -0.5528078048062071 2117 9 -0.07182423049771103 2117 10-11 -0.04117165325305905 2117 12-13 -0.25929540072094426 2117 14-15 -0.07925488302571893 2117 16-17 -0.15776828523812725 2117 18-19 -0.05724928219432712 2117 20-21 -0.22007557994989213 2117 22-23 0.065813978077081 2117 24-25 -0.1784310284953321 2117 26-27 -0.029057269647161377 2117 28-29 -1.1937246414086147 2117 30-31 -0.6243781558909234 2117 32-33 -0.7865385171622279 2117 34-35 -0.23438659993306743 2117 36-37 -0.38680362090899223 2117 38-39 0.10650250461399224 2117 40-41 -0.2967236020236257 2117 42-43 -0.036366597217913466 2117 44-45 -0.23575520882418743 2117 46-47 -0.4488994895088396 2117 48-49 -0.30585656513719783 2117 50-51 -0.3330217582005943 2117 52-53 0.009248441781863903 2117 54-55 -0.37219878305144505 2117 56-57 -0.37022247566289224 2117 58-59 0.07286929500128281 2117 60-61 -0.6987306269784312 2117 62-63 -0.34328996722115335 2117 64-65 -0.23795593888011268 2117 66-67 -0.10413378277928587 2117 68-69 -0.18108439389691 2117 70-71 -0.2696086536410718 2117 72-73 -0.6498791069475658 2117 74-75 0.06546177785210716 2117 76 -0.7273622654353247 2118 1 -0.034520083744524754 2118 2 -0.31173301924722807 2118 3 -0.2643553582830904 2118 4 -0.20939333426827744 2118 5 -0.10633101771158238 2118 6 -0.02256633567770905 2118 7 -0.1858360487815176 2118 8 -0.34243046512075637 2118 9 -0.16776379236108596 2118 10-11 -0.29459521854498405 2118 12-13 -0.11920330830960069 2118 14-15 -0.10037434402597256 2118 16-17 -0.12531667362093657 2118 18-19 -0.26949039687131204 2118 20-21 -0.24806210495637515 2118 22-23 -0.20803137843218167 2118 24-25 -0.16717861156786995 2118 26-27 -0.571445692315443 2118 28-29 0.010293936541039272 2118 30-31 -0.13343789635776915 2118 32-33 -0.24098068033723052 2118 34-35 -0.3997542719086695 2118 36-37 -0.4986101869783184 2118 38-39 -0.808601049515218 2118 40-41 -0.47416096124383245 2118 42-43 -0.3419260287167063 2118 44-45 -0.33134593579610794 2118 46-47 -0.22629299519982737 2118 48-49 -0.13198728852110264 2118 50-51 -0.2711683485886951 2118 52-53 -0.4798180760485806 2118 54-55 -0.09785509098296075 2118 56-57 -0.17905098843024803 2118 58-59 -0.4497252392543061 2118 60-61 -0.08703369473213485 2118 62-63 -0.5817036654690071 2118 64-65 -0.34711135977432406 2118 66-67 -0.36298920566683535 2118 68-69 -0.20769551275990494 2118 70-71 -0.7939274289316032 2118 72-73 -0.25061265689075185 2118 74-75 0.17316205891336267 2118 76 -1.2333323254349224 2119 1 0.020629569624233568 2119 2 -0.0402605629393733 2119 3 -0.019969954156721315 2119 4 -0.1381367441234005 2119 5 -0.3503428073250987 2119 6 -0.41654283947612214 2119 7 -0.1320770716550328 2119 8 -0.2604220795505725 2119 9 -0.44477819727619305 2119 10-11 -0.14786849680476166 2119 12-13 -0.5967241684532283 2119 14-15 -1.0093058065192295 2119 16-17 -0.1772283345450063 2119 18-19 -0.3834324451090936 2119 20-21 -0.20529362141616048 2119 22-23 -0.09143251588081114 2119 24-25 -0.46292563302994694 2119 26-27 -0.3171387472367755 2119 28-29 -0.18623767539802571 2119 30-31 -0.4007694584815624 2119 32-33 -0.727582448779259 2119 34-35 -0.07988835800606608 2119 36-37 -0.15773883490891194 2119 38-39 -0.23229028526597517 2119 40-41 -0.6364964670763769 2119 42-43 -0.49413242997869844 2119 44-45 -0.27446312621496816 2119 46-47 -0.14365154059398577 2119 48-49 -0.3384819541361068 2119 50-51 -0.4293318256944261 2119 52-53 -0.15417151537774032 2119 54-55 -0.10180917791059585 2119 56-57 0.013370863867478988 2119 58-59 -0.1965806504378378 2119 60-61 -0.2043382735855488 2119 62-63 -0.13817134781364615 2119 64-65 -0.3144927371664963 2119 66-67 0.08480854434354512 2119 68-69 -0.1779724026266507 2119 70-71 0.3470809151258507 2119 72-73 0.5535925820149714 2119 74-75 0.6099693153374588 2119 76 -0.5036424254805922 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 0.0 22 8.0 23 18.0 24 23.0 25 59.0 26 103.0 27 199.0 28 354.0 29 592.0 30 963.0 31 1523.0 32 2272.0 33 3628.0 34 6000.0 35 9304.0 36 14219.0 37 59402.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.8861952588959 15.080724442327378 10.760218508346087 33.27286179043063 2 22.886620924505163 18.808338991983298 31.5104034701882 26.794636613323338 3 20.331613779403863 17.929643555726724 30.094558574628305 31.644184090241108 4 15.880367694007235 21.172810102463792 31.012780103173238 31.934042100355736 5 16.42461158013155 29.099311840598364 36.441030110774406 18.03504646849568 6 30.37934913701365 34.89444506379917 16.47427256787846 18.25193323130872 7 29.692203224923734 24.584215913812848 20.3285733107663 25.395007550497116 8 25.531828639187587 33.94885931751614 15.939150087666846 24.58016195562943 9 23.637616677983967 16.412449705581285 16.56345964791373 43.38647396852102 10-11 27.232464097132837 24.810224082538586 23.096920005270146 24.860391815058428 12-13 26.24583202424267 18.82506156948991 24.82086572277007 30.10824068349735 14-15 23.463803220869778 18.28436489677609 20.43245598921647 37.81937589313766 16-17 27.653062258662803 24.521886306742747 19.84209832875574 27.982953105838714 18-19 29.358258419564404 23.35890705287375 20.292087687115508 26.990746840446338 20-21 25.205991750195096 21.924312600715524 20.17198917593165 32.69770647315773 22-23 24.420030607384284 25.462404605296495 20.216075971176355 29.901488816142862 24-25 27.745796552108565 25.308860939099414 20.233305293455896 26.712037215336125 26-27 27.56438192340046 27.554753772714836 20.589546868824048 24.29131743506066 28-29 25.49382278121801 25.629630380362627 21.719587712452746 27.15695912596662 30-31 24.613607110642654 25.953947035036336 20.81251456891222 28.61993128540879 32-33 22.995064305911683 29.355217950926836 21.886306742745948 25.76341100041553 34-35 27.14530399618928 23.385257781065988 25.13808795062279 24.331350272121945 36-37 29.313158134773843 22.868884857452695 23.669034853905483 24.148922153867982 38-39 24.020208981544357 23.5301867861233 26.02134408983571 26.42826014249663 40-41 23.822578520102567 19.93939332515785 26.855952730847584 29.382075423892 42-43 27.12047350231582 19.4640667281517 26.2042789528626 27.211180816669877 44-45 22.547101926643627 23.396912910843326 28.86519575550578 25.19078940700727 46-47 25.244504352937597 20.40863898488887 27.33381305171837 27.013043610455156 48-49 24.671882759529336 19.865408588310412 24.596377788363114 30.866330863797142 50-51 23.079183938217675 20.58295918677599 27.322157921941038 29.0156989530653 52-53 20.421307604212064 20.651876475894152 32.594837284253416 26.331978635640375 54-55 20.83278435982933 21.334968429800647 28.95235585644934 28.879891353920684 56-57 23.31786072626661 22.467542997293982 24.90954605803241 29.305050218406997 58-59 20.924505163729236 22.504535365717704 29.14643910448064 27.424520366072425 60-61 23.63913691230275 25.058529021273145 25.212072687470226 26.090261378953876 62-63 20.41066596398058 31.221052204846504 21.765194742016234 26.603087089156674 64-65 20.07874813771296 32.90395159573929 20.598668274736745 26.418631991811004 66-67 20.199353393669746 32.333863726195666 20.01793876496164 27.448844115172953 68-69 19.610515967527796 32.64804548541082 20.249014381416654 27.49242416564473 70-71 19.786356403733695 31.7044867182195 20.567250098815233 27.941906779231573 72-73 20.055437878158287 30.246075261733672 20.542419604941774 29.156067255166263 74-75 20.37316685078393 30.235433621502196 20.763360325938237 28.628039201775636 76 20.385328725334197 30.68846344849953 20.477556274007032 28.448651552159237 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 2.0 16 5.5 17 7.0 18 5.5 19 7.0 20 14.0 21 18.0 22 19.5 23 34.5 24 53.0 25 58.0 26 89.0 27 143.5 28 220.5 29 274.0 30 341.5 31 474.0 32 665.5 33 792.0 34 919.5 35 1201.5 36 1525.0 37 1694.0 38 2039.0 39 3819.5 40 6101.0 41 6751.0 42 6555.0 43 5998.0 44 5299.0 45 4998.0 46 4839.0 47 4790.5 48 4524.5 49 4104.5 50 3902.0 51 3687.5 52 3078.5 53 2443.0 54 2202.0 55 2085.5 56 1857.5 57 1706.0 58 1666.0 59 1660.5 60 1635.5 61 1687.5 62 1759.0 63 1765.0 64 1838.0 65 1999.0 66 2168.5 67 2244.0 68 2267.5 69 2304.0 70 2336.0 71 2355.0 72 2202.5 73 1990.0 74 1796.5 75 1663.0 76 1461.0 77 1075.0 78 714.5 79 538.0 80 422.0 81 221.0 82 88.0 83 40.0 84 24.5 85 6.0 86 1.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 98669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.869381467330165 #Duplication Level Percentage of deduplicated Percentage of total 1 68.0728423767561 35.308962288054 2 18.35713867015768 19.043468566621733 3 6.01027765294359 9.352481529153026 4 2.588952500048848 5.3714945930332725 5 1.5318783094628656 3.972879019752911 6 0.9183454151116668 2.858040519312043 7 0.681920318880791 2.4759549605245823 8 0.4181402528380781 1.7350941025043327 9 0.30872037359073057 1.4411821342062858 >10 1.0805213075675568 7.78765367035239 >50 0.01563141132104965 0.6050532588756347 >100 0.007815705660524825 0.8199130425969656 >500 0.0 0.0 >1k 0.007815705660524825 9.227822315012821 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 4873 4.938734556953045 TruSeq Adapter, Index 5 (95% over 23bp) CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG 1675 1.6975949893076852 TruSeq Adapter, Index 5 (96% over 29bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 1347 1.3651704182671356 TruSeq Adapter, Index 5 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 1210 1.2263223504849547 RNA PCR Primer, Index 5 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 334 0.3385055083156817 RNA PCR Primer, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 201 0.20371139871692223 TruSeq Adapter, Index 5 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT 153 0.1550639005158662 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT 121 0.12263223504849548 No Hit TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 100 0.10134895458553345 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0030404686375660036 0.0 2 0.0 0.0 0.0 0.0030404686375660036 0.0 3 0.0 0.0 0.0 0.0030404686375660036 0.0 4 0.0 0.0 0.0 0.01621583273368535 0.0 5 0.0 0.0 0.0 0.01824281182539602 0.0 6 0.0 0.0 0.0 0.01824281182539602 0.0 7 0.0 0.0 0.0 0.019256301371251356 0.0 8 0.0 0.0 0.0 0.019256301371251356 0.0 9 0.0 0.0 0.0 0.019256301371251356 0.0 10 0.0 0.0 0.0 0.019256301371251356 0.0 11 0.0 0.0 0.0 0.019256301371251356 0.0 12 0.0 0.0 0.0 0.019256301371251356 0.0 13 0.0 0.0 0.0 0.019256301371251356 0.0 14 0.0 0.0 0.0 0.019256301371251356 0.0 15 0.0 0.0 0.0 0.02026979091710669 0.0 16 0.0 0.0 0.0 0.02026979091710669 0.0 17 0.0 0.0 0.0 0.023310259554672693 0.0 18 0.0 0.0 0.0 0.026350728192238698 0.0 19 0.0 0.0 0.0 0.0324316654673707 0.0 20 0.0 0.0 0.0 0.03547213410493671 0.0 21 0.0 0.0 0.0 0.03749911319664738 0.0 22 0.0 0.0 0.0 0.04053958183421338 0.0 23 0.0 0.0 0.0 0.04256656092592405 0.0 24 0.0 0.0 0.0 0.04763400865520072 0.0 25 0.0 0.0 0.0 0.05067447729276672 0.0 26 0.0 0.0 0.0 0.0557419250220434 0.0 27 0.0 0.0 0.0 0.14188853641974683 0.0 28 0.0 0.0 0.0 0.21587327326718625 0.0 29 0.0 0.0 0.0 0.3050603533024557 0.0 30 0.0 0.0 0.0 0.38411253787917177 0.0 31 0.0 0.0 0.0 0.49863685656082457 0.0 32 0.0 0.0 0.0 0.6283635184303074 0.0 33 0.0 0.0 0.0 0.7702520548500542 0.0 34 0.0 0.0 0.0 0.9678825162918444 0.0 35 0.0 0.0 0.0 1.2384842250352188 0.0 36 0.0 0.0 0.0 1.7036759265828172 0.0 37 0.0 0.0 0.0 2.30264824818332 0.0 38 0.0 0.0 0.0 2.96952436935613 0.0 39 0.0 0.0 0.0 3.6840344991841407 0.0 40 0.0 0.0 0.0 4.4654349390386034 0.0 41 0.0 0.0 0.0 5.4343309448763035 0.0 42 0.0 0.0 0.0 6.6069383494309255 0.0 43 0.0 0.0 0.0 7.7420466407889 0.0 44 0.0 0.0 0.0 8.657227700696268 0.0 45 0.0 0.0 0.0 9.607880894708572 0.0 46 0.0 0.0 0.0 10.573736431908705 0.0 47 0.0 0.0 0.0 11.387568537230539 0.0 48 0.0 0.0 0.0 12.21052204846507 0.0 49 0.0 0.0 0.0 12.927059157384791 0.0 50 0.0 0.0 0.0 13.625353454479116 0.0 51 0.0 0.0 0.0 14.381416655687197 0.0 52 0.0 0.0 0.0 15.346258703341475 0.0 53 0.0 0.0 0.0 16.37697757147635 0.0 54 0.0 0.0 0.0 17.40870992915708 0.0 55 0.0 0.0 0.0 18.185042921282268 0.0 56 0.0 0.0 0.0 18.955294976132322 0.0 57 0.0 0.0 0.0 19.681966980510595 0.0 58 0.0 0.0 0.0 20.327559821220444 0.0 59 0.0 0.0 0.0 20.91335677872483 0.0 60 0.0 0.0 0.0 21.544760765792702 0.0 61 0.0 0.0 0.0 22.193394075140116 0.0 62 0.0 0.0 0.0 22.86331066495049 0.0 63 0.0 0.0 0.0 23.57376683659508 0.0 64 0.0 0.0 0.0 24.338951443715857 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAACCG 20 7.892799E-5 70.00001 18 AGTCTTG 20 7.892799E-5 70.00001 11 TTAACTC 20 7.892799E-5 70.00001 68 TAACTCC 20 7.892799E-5 70.00001 69 GTAAACC 20 7.892799E-5 70.00001 17 CGATTAC 15 0.0022188642 70.0 69 GTATCAG 15 0.0022188642 70.0 1 AAAAGTC 25 2.3803007E-4 56.000004 60 ACCGAAG 25 2.3803007E-4 56.000004 21 TTTCCAA 25 2.3803007E-4 56.000004 39 CTTATAT 25 2.3803007E-4 56.000004 1 TTCCAAG 25 2.3803007E-4 56.000004 40 TCAGAGA 25 2.3803007E-4 56.000004 53 TCCAAGG 25 2.3803007E-4 56.000004 41 GTCTTTA 25 2.3803007E-4 56.000004 64 GGACAAA 25 2.3803007E-4 56.000004 46 TGTAAAC 25 2.3803007E-4 56.000004 16 TTAATAC 20 0.0069333063 52.500004 3 GTCCTTC 20 0.0069333063 52.500004 7 TCCCTAT 20 0.0069333063 52.500004 54 >>END_MODULE