FastQCFastQC Report
Wed 25 May 2016
SRR1780187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526451
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATT250.002337682835.24257314
ATTAGAG300.005687056629.39119518
TGTACAC508.3991414E-526.369173
ATATAAG500.002549175222.04339617
TCGGATA500.002567566722.01612937
ACGAGAG500.002574669222.00565724
TATGCAG703.230507E-521.9826630
GTGTACA500.002591781621.9805722
CATCGTT500.002591781621.98057232
GGATTAC703.2328775E-521.9805721
CGTTGAA500.002594642621.97639742
ATAGCGG500.00259607421.9743086
CTGCCGA5500.021.64260918
TTACTCG1057.5659045E-820.99371319
AAACAAT856.1494684E-620.68759732
GACAGCT755.5144992E-520.5093547
TCCGTAT654.896484E-420.3264513
GCAGTGT654.956514E-420.28975933
TAGATCT2300.020.10168339
TTACATT550.004500794719.98423628
Produced by FastQC (version 0.11.5)