FastQCFastQC Report
Wed 25 May 2016
SRR1780187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526451
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT176083.344660756651616No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG47610.9043576705144449No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC43570.8276173850937695No Hit
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG41850.7949457784295214TruSeq Adapter, Index 9 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC11090.21065588250378475No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT9470.17988378785490006No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT5680.10789228247263277TruSeq Adapter, Index 9 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG4300.037.34964444
GTATACG301.2999718E-436.66745844
GTCTTCT4650.034.5383843
CTTTATA1850.033.3011782
ATGCCGT27500.032.08069244
CGTCTTC5150.031.1851442
GTTCTAA300.00574149929.33396739
CCGTCTT5650.028.42539241
TCTTTAT2100.028.3320751
ATGTATA407.025936E-427.50059542
TATGCCG32500.027.145243
TTTATAC2600.027.080083
ATCGTAT2300.026.78318839
ATATCGT2300.026.78318837
CTGATAT2400.025.66478334
TATCGTA2750.025.60055238
TAGACAC450.001397763824.44265210
ACACACG450.001397763824.44265213
AGCATAT450.001397763824.44265220
AGTAACG450.001397763824.44265212