FastQCFastQC Report
Wed 25 May 2016
SRR1780186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182209
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAG252.3856823E-455.9999963
TATGCAT200.006942698752.54
ATAGTGC200.006942698752.526
AGACTGG501.704851E-435.051
GGGAATC400.00241411235.029
AACGCTC601.2109469E-535.07
CGATGTA501.704851E-435.064
ATAACGC601.2109469E-535.05
TGGGAAT400.00241411235.028
ACTGGGG400.00241411235.053
CATAACG652.0943779E-532.3076934
TGTAACA552.9836487E-431.8181867
GTAACAC552.9836487E-431.8181868
CTGTAAG450.00429904531.1111094
TTCCGGA450.00429904531.11110938
GAGAGGT450.00429904531.1111096
TACAGGG450.00429904531.1111095
GTGTATG802.4535893E-630.6251
AGTACCG703.474913E-530.05
ATGTAAC604.9680436E-429.16666666
Produced by FastQC (version 0.11.5)