##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780186_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 182209 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.73931584060063 34.0 32.0 34.0 21.0 34.0 2 30.910020910053838 34.0 33.0 34.0 21.0 34.0 3 30.702715014077242 34.0 32.0 34.0 21.0 34.0 4 30.54913862652229 34.0 32.0 34.0 21.0 34.0 5 30.555422619080286 34.0 32.0 34.0 21.0 34.0 6 33.72288964870012 38.0 34.0 38.0 21.0 38.0 7 34.02419199929751 38.0 34.0 38.0 21.0 38.0 8 34.06488702533903 38.0 35.0 38.0 21.0 38.0 9 34.21664681766543 38.0 35.0 38.0 21.0 38.0 10-11 33.99981340109434 38.0 34.5 38.0 21.0 38.0 12-13 34.05228336690284 38.0 34.5 38.0 21.0 38.0 14-15 33.983853706457964 38.0 34.5 38.0 21.0 38.0 16-17 34.04166369388999 38.0 35.0 38.0 21.0 38.0 18-19 34.05423442310753 38.0 35.0 38.0 21.0 38.0 20-21 34.074806952455695 38.0 35.0 38.0 21.5 38.0 22-23 34.088390255146564 38.0 35.0 38.0 21.0 38.0 24-25 34.09272593560142 38.0 35.0 38.0 21.5 38.0 26-27 34.08666421526928 38.0 35.0 38.0 21.0 38.0 28-29 34.16828751598439 38.0 35.5 38.0 22.0 38.0 30-31 34.17818549028863 38.0 35.5 38.0 22.0 38.0 32-33 34.25755862772969 38.0 36.0 38.0 22.0 38.0 34-35 34.19977333721167 38.0 35.5 38.0 22.0 38.0 36-37 34.121774994649 38.0 35.0 38.0 21.5 38.0 38-39 34.1449242353561 38.0 35.5 38.0 22.0 38.0 40-41 34.1994687419392 38.0 35.5 38.0 22.0 38.0 42-43 34.2530528129785 38.0 36.0 38.0 22.0 38.0 44-45 34.312402241382145 38.0 36.0 38.0 22.5 38.0 46-47 34.248376863930986 38.0 36.0 38.0 21.5 38.0 48-49 34.2225466360059 38.0 36.0 38.0 21.5 38.0 50-51 34.1544627323568 38.0 35.5 38.0 21.5 38.0 52-53 34.196584691206255 38.0 36.0 38.0 22.0 38.0 54-55 34.18413744655862 38.0 36.0 38.0 22.0 38.0 56-57 34.13920003951506 38.0 35.5 38.0 22.0 38.0 58-59 34.2109692715508 38.0 36.0 38.0 22.0 38.0 60-61 34.20282477813939 38.0 36.0 38.0 22.0 38.0 62-63 34.09844738734091 38.0 35.0 38.0 22.0 38.0 64-65 34.05298311279904 38.0 35.0 38.0 22.0 38.0 66-67 33.967586672447574 38.0 35.0 38.0 22.0 38.0 68-69 33.90002689219523 38.0 35.0 38.0 21.0 38.0 70-71 33.86941369526203 38.0 35.0 38.0 20.0 38.0 72-73 33.92316515649611 38.0 35.0 38.0 20.5 38.0 74-75 33.94886366754661 38.0 35.0 38.0 20.5 38.0 76 32.30893644111981 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3199423851668506 1101 2 -0.23413197772722327 1101 3 0.022593193978643455 1101 4 -0.28378968247612235 1101 5 -0.4973449962664809 1101 6 -0.273598894348801 1101 7 -0.3146600792305989 1101 8 -0.8832566201335297 1101 9 -0.40779100360684595 1101 10-11 -0.5348369768308316 1101 12-13 -0.09609115728144246 1101 14-15 -0.5054509008473431 1101 16-17 -0.25165716004744354 1101 18-19 -0.17995753307942408 1101 20-21 -0.05689646982616381 1101 22-23 0.0025330379790489133 1101 24-25 -0.3471621818836894 1101 26-27 -0.6967894606153564 1101 28-29 -1.0040668947542812 1101 30-31 -0.4881487869074661 1101 32-33 -0.7551296312514637 1101 34-35 -0.49824773385027754 1101 36-37 -0.5813624069500491 1101 38-39 -0.2016615184777777 1101 40-41 -0.3600979675795557 1101 42-43 -0.4645654540709785 1101 44-45 -0.3520448767282147 1101 46-47 -0.4411844843303854 1101 48-49 -0.32182767733327466 1101 50-51 -0.44676792673150345 1101 52-53 -0.29087952906751013 1101 54-55 -0.2543114398417501 1101 56-57 -0.04882949145281401 1101 58-59 0.01351082259589731 1101 60-61 -0.5237332262064669 1101 62-63 -0.24530596729999843 1101 64-65 0.021755360107995614 1101 66-67 -0.22526066022737012 1101 68-69 -0.35520757404209036 1101 70-71 -0.12671212472177018 1101 72-73 -0.43366228646489446 1101 74-75 -0.3428670515627772 1101 76 -0.6776458572883932 1102 1 0.9464928678479154 1102 2 0.7041977867267413 1102 3 0.8196178755970358 1102 4 0.9201593764667386 1102 5 0.5013206185199479 1102 6 0.548697961280638 1102 7 0.3781608381592392 1102 8 0.9266340594562408 1102 9 0.5929528259885544 1102 10-11 0.8470271052158296 1102 12-13 0.8590964906392102 1102 14-15 0.5655442529901364 1102 16-17 0.44076704822484203 1102 18-19 0.4817487669318439 1102 20-21 0.8422312209210503 1102 22-23 0.7827381546250507 1102 24-25 0.5930812532566208 1102 26-27 0.46221651256169594 1102 28-29 0.2776191189952968 1102 30-31 0.983263362309259 1102 32-33 0.4325273055230241 1102 34-35 0.4507345720214673 1102 36-37 0.6743369055710815 1102 38-39 0.58013043463842 1102 40-41 0.6510211578571585 1102 42-43 0.44006882971127936 1102 44-45 0.674588730845322 1102 46-47 0.4685686994856937 1102 48-49 0.6095326292146837 1102 50-51 0.34027140607589246 1102 52-53 0.7217926224032496 1102 54-55 0.23364528279996932 1102 56-57 0.8073814856687918 1102 58-59 0.5507518910056817 1102 60-61 0.42934465049814463 1102 62-63 0.4766527839678929 1102 64-65 0.9175696281120551 1102 66-67 0.813634865524925 1102 68-69 0.8307588409246662 1102 70-71 0.6743788253514893 1102 72-73 0.6987822080690833 1102 74-75 0.5016928492599391 1102 76 0.3825953238228408 1103 1 0.027428540362535614 1103 2 0.07127597602715241 1103 3 0.45910238935055503 1103 4 0.7881342519269943 1103 5 0.6435904907313414 1103 6 -0.14005230945793556 1103 7 -0.24477613390054387 1103 8 -0.23735554418249194 1103 9 0.06590062759132564 1103 10-11 0.14297252431914842 1103 12-13 0.48005815405701213 1103 14-15 0.03884726360308832 1103 16-17 0.2648367904817235 1103 18-19 -0.23013773945758942 1103 20-21 0.2521640777544931 1103 22-23 0.357561633074269 1103 24-25 0.12716832278228907 1103 26-27 -0.17839210775452585 1103 28-29 0.07957926627708645 1103 30-31 0.022009301230639267 1103 32-33 0.16940839714095546 1103 34-35 0.08728861144100364 1103 36-37 -0.028073750699221023 1103 38-39 -0.2904803515864032 1103 40-41 0.19696224514328264 1103 42-43 0.07808485743491644 1103 44-45 0.06471651945264512 1103 46-47 0.0774684179175722 1103 48-49 0.05742303423959072 1103 50-51 -0.3371923149550824 1103 52-53 0.04537720113402344 1103 54-55 0.16513542791584257 1103 56-57 0.10196888008074723 1103 58-59 0.34249761333619944 1103 60-61 0.22344471547540223 1103 62-63 -0.0870812424561862 1103 64-65 -0.030013217891848853 1103 66-67 -0.25328737835639004 1103 68-69 0.14436724465003437 1103 70-71 -0.10823326041848702 1103 72-73 0.03336743657243346 1103 74-75 -0.05128960688041673 1103 76 -0.022122025105034027 1104 1 0.24965076258475705 1104 2 0.04909574591041377 1104 3 0.43777039976459875 1104 4 0.8057877330725383 1104 5 -0.02963534281195379 1104 6 0.42680752089395924 1104 7 0.3629084214030627 1104 8 -0.2745689480625586 1104 9 0.3266800010691142 1104 10-11 0.1521184048935993 1104 12-13 0.32557339719130596 1104 14-15 0.22748839906962814 1104 16-17 -0.007847344900824282 1104 18-19 0.13997647906932542 1104 20-21 0.06809588257518584 1104 22-23 0.07057918577435629 1104 24-25 0.22341422855200932 1104 26-27 -0.029534293023694147 1104 28-29 0.032774865504428874 1104 30-31 0.2810292054886361 1104 32-33 0.40077649993763487 1104 34-35 0.2669644323866649 1104 36-37 0.02348835562478513 1104 38-39 0.38815406514325446 1104 40-41 0.14933479910312997 1104 42-43 0.11365636999055084 1104 44-45 0.45993781784854093 1104 46-47 0.41928835576925394 1104 48-49 0.3792211361964206 1104 50-51 0.3148149076870581 1104 52-53 0.12224375022279332 1104 54-55 0.05434345680641428 1104 56-57 0.22084013228533195 1104 58-59 0.11908701452877324 1104 60-61 0.43082692761951336 1104 62-63 0.3192091749453425 1104 64-65 0.09950303305297581 1104 66-67 0.18926322213071956 1104 68-69 0.11818096767010644 1104 70-71 0.43398140322474177 1104 72-73 0.3299744744493154 1104 74-75 0.2572137944801369 1104 76 0.19458243445657075 1105 1 -0.052236029868073075 1105 2 0.15870512425294692 1105 3 0.08387915773319321 1105 4 0.2515242666796915 1105 5 0.5555013533759556 1105 6 0.44073812458197636 1105 7 0.5671269641660786 1105 8 0.20347837149419234 1105 9 -0.03527182368594595 1105 10-11 0.5521269617754712 1105 12-13 -0.4173329477366039 1105 14-15 -0.006963746568501961 1105 16-17 0.22921678892880237 1105 18-19 0.46708254162013674 1105 20-21 0.18965922489324782 1105 22-23 0.3452935998418667 1105 24-25 0.13741756573593733 1105 26-27 0.5029690227680277 1105 28-29 0.3785349305367305 1105 30-31 0.39162262524647673 1105 32-33 0.3406173419348093 1105 34-35 0.3129854823160727 1105 36-37 0.31055890711581924 1105 38-39 0.43238244832042483 1105 40-41 0.42549318698658567 1105 42-43 0.4950815186663746 1105 44-45 0.23037379097993949 1105 46-47 0.43861835191865595 1105 48-49 0.4302586581234351 1105 50-51 0.5470572385816865 1105 52-53 0.6572841814896364 1105 54-55 0.6429093234046235 1105 56-57 0.1694624743038986 1105 58-59 0.5226552342695001 1105 60-61 0.3211619295448074 1105 62-63 0.36640893535265207 1105 64-65 0.1682746926602121 1105 66-67 0.41120520647162806 1105 68-69 0.20122336701978583 1105 70-71 0.16080936711470173 1105 72-73 0.39970022638531333 1105 74-75 0.31034561324537435 1105 76 0.46180529844492924 1106 1 -0.24931830958018963 1106 2 -0.015389842139239107 1106 3 0.24696459290577266 1106 4 0.14553105308095482 1106 5 -0.17863597399226094 1106 6 -0.056424122278777133 1106 7 -0.12145934632258815 1106 8 -0.07692535454268068 1106 9 -0.199450863647904 1106 10-11 -0.4639766929393545 1106 12-13 0.03170636584268749 1106 14-15 0.19970594588216528 1106 16-17 0.011803401995869933 1106 18-19 0.31787464864643766 1106 20-21 0.2527792404544229 1106 22-23 0.4511809110005345 1106 24-25 0.3100752847270556 1106 26-27 0.07705102151449239 1106 28-29 0.10995769740006978 1106 30-31 0.3264321089180555 1106 32-33 0.20839274231087046 1106 34-35 0.2681952339856579 1106 36-37 0.39369664512615543 1106 38-39 0.12780270810559102 1106 40-41 0.3430124763596041 1106 42-43 0.047708547562614 1106 44-45 0.029325572950583023 1106 46-47 0.171802557326167 1106 48-49 -0.023598135842291867 1106 50-51 0.007661530872418609 1106 52-53 0.19219956760129264 1106 54-55 0.08744886391771445 1106 56-57 0.43225397617340633 1106 58-59 0.3713487439628196 1106 60-61 0.08498219797508 1106 62-63 -0.010835007676163855 1106 64-65 0.33495432697259986 1106 66-67 0.0686972637886214 1106 68-69 0.10716687130990721 1106 70-71 -0.06760706721497911 1106 72-73 0.06412132906745427 1106 74-75 -0.1681280948529178 1106 76 0.5102567454180686 1107 1 -0.2869346032689002 1107 2 -0.8348266035171612 1107 3 -0.6540663349292792 1107 4 -1.079802112621838 1107 5 -0.5298261508424815 1107 6 -1.0269588637086642 1107 7 -0.49650754010349374 1107 8 -0.6638836983529188 1107 9 -0.30773181243677783 1107 10-11 -0.503933528074711 1107 12-13 -0.49208878963464286 1107 14-15 -1.1513941295515764 1107 16-17 -0.6684413385958692 1107 18-19 -0.24886954714603604 1107 20-21 -0.17830365284874006 1107 22-23 -0.4695361286440942 1107 24-25 -0.2585212285576617 1107 26-27 -0.38131793330907726 1107 28-29 -0.2992850316251534 1107 30-31 -0.5315890125302758 1107 32-33 -0.6475518557949016 1107 34-35 -0.36888673734951993 1107 36-37 -0.2015531243818458 1107 38-39 -0.6272764973256386 1107 40-41 -0.6079283500589696 1107 42-43 -0.5847300577512797 1107 44-45 -0.6487232075372162 1107 46-47 -0.4469469188283881 1107 48-49 -0.28958589881270314 1107 50-51 -0.17057583397545528 1107 52-53 -0.5272916737028694 1107 54-55 -0.2900111142903157 1107 56-57 -0.3840669524998006 1107 58-59 -0.42875602512611266 1107 60-61 -0.23503917491905923 1107 62-63 -0.20979779369577045 1107 64-65 -0.376670851134854 1107 66-67 -0.2339282495799111 1107 68-69 -0.7341833927081964 1107 70-71 -0.5680345250978078 1107 72-73 -0.6068639194448195 1107 74-75 -0.17324082639260752 1107 76 -0.42501272067865514 1108 1 -0.1698407628389731 1108 2 -0.1267344166630906 1108 3 -0.14982904679368758 1108 4 -0.1573651552014148 1108 5 0.3429174071015737 1108 6 0.5269718239032954 1108 7 0.7749717212992948 1108 8 0.013324514407173638 1108 9 -0.04159222307477961 1108 10-11 0.36503780994375745 1108 12-13 -0.08309574843304546 1108 14-15 0.2575558497787256 1108 16-17 0.44365732476364883 1108 18-19 0.6840658944965199 1108 20-21 -0.06737627238849342 1108 22-23 0.37276268629320697 1108 24-25 0.3134782202503814 1108 26-27 0.24101223067052757 1108 28-29 -0.020485252101941853 1108 30-31 0.37588825080167965 1108 32-33 0.29907726091999365 1108 34-35 0.49270379592869773 1108 36-37 0.010741901110776553 1108 38-39 0.07422722322004205 1108 40-41 0.3244645120052425 1108 42-43 0.7078409082126171 1108 44-45 0.39246116617092497 1108 46-47 0.12413961830817044 1108 48-49 0.3823515409006717 1108 50-51 0.25509076379221796 1108 52-53 0.2433091255254567 1108 54-55 0.19269523819694 1108 56-57 0.18064642768484873 1108 58-59 0.10605519163012644 1108 60-61 0.3883589234687008 1108 62-63 0.4557406519429179 1108 64-65 0.40140548895058004 1108 66-67 0.44009338470421966 1108 68-69 0.5022262807105733 1108 70-71 0.6499274426574644 1108 72-73 0.32853466123589214 1108 74-75 0.3770296021591406 1108 76 0.08773359322515972 1109 1 0.845484095918092 1109 2 0.7104477867267391 1109 3 0.3188503317373872 1109 4 0.5902690255895457 1109 5 0.10884254834450857 1109 6 0.6606935753157259 1109 7 0.6694985574574872 1109 8 0.6123358138422006 1109 9 0.6572072119534695 1109 10-11 0.3268845613561808 1109 12-13 0.18484210467429563 1109 14-15 0.48280302491996707 1109 16-17 0.2360302061195796 1109 18-19 0.17656236342306642 1109 20-21 0.4600601682894663 1109 22-23 0.5188785055022436 1109 24-25 -0.1472915537609154 1109 26-27 0.4535651967722245 1109 28-29 -0.0029181617064608645 1109 30-31 0.4144475728355772 1109 32-33 0.24429265640021924 1109 34-35 0.3434100106179656 1109 36-37 0.36536760732546725 1109 38-39 0.48077736446298047 1109 40-41 0.41572510522558304 1109 42-43 0.5085118121674199 1109 44-45 0.459533906283923 1109 46-47 0.43999413808218435 1109 48-49 0.49539885728485444 1109 50-51 0.5003591253741391 1109 52-53 0.3813211311751772 1109 54-55 0.5785356336771628 1109 56-57 0.31161394180914215 1109 58-59 0.39344925942673825 1109 60-61 0.4079740364630524 1109 62-63 0.6366198892310564 1109 64-65 0.5576025228488888 1109 66-67 0.6716611813143913 1109 68-69 0.4427983146088792 1109 70-71 0.6505630358778021 1109 72-73 0.11043790982346735 1109 74-75 0.6257937264529261 1109 76 0.25125760452459645 1110 1 0.0624079642308466 1110 2 0.3860907908419655 1110 3 0.2471682329719549 1110 4 0.17085030130970935 1110 5 0.1662242355873076 1110 6 0.35583246420461734 1110 7 0.18114979202538706 1110 8 0.5383646204265631 1110 9 0.33544795269421 1110 10-11 0.43206717452490295 1110 12-13 0.4913750264850023 1110 14-15 0.23123409487881474 1110 16-17 0.36360222258048225 1110 18-19 0.19887460622143038 1110 20-21 0.18220523001786404 1110 22-23 0.2608538141442267 1110 24-25 0.6076209976794118 1110 26-27 0.659056451916257 1110 28-29 0.5065339987873685 1110 30-31 0.2401162971154065 1110 32-33 0.3052108662767594 1110 34-35 0.021444990041835865 1110 36-37 0.4071114344859552 1110 38-39 0.2743344632284135 1110 40-41 0.404781175184425 1110 42-43 0.5132700426200927 1110 44-45 0.7910539680123136 1110 46-47 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-0.3209078211433365 2116 8 -0.24764361002610968 2116 9 -0.785951224260522 2116 10-11 -0.11114270201830578 2116 12-13 -0.3913410228977128 2116 14-15 -0.1411938886602826 2116 16-17 -0.26602740704915107 2116 18-19 -0.15194843904606614 2116 20-21 -0.4453667864430386 2116 22-23 -0.14964001301627405 2116 24-25 -0.5878522533493893 2116 26-27 -0.43662256042942715 2116 28-29 -0.7105441905130405 2116 30-31 -0.5613240321026964 2116 32-33 -0.5116615617067808 2116 34-35 -0.32011879596640114 2116 36-37 -0.29144823629593475 2116 38-39 -0.4159947548785823 2116 40-41 -0.67649454498018 2116 42-43 -0.32932254997249544 2116 44-45 -0.27478965338686123 2116 46-47 -0.2939307590371598 2116 48-49 -0.7254987764600003 2116 50-51 -0.600566800551789 2116 52-53 -0.6490672433104194 2116 54-55 -0.38939132105534924 2116 56-57 -0.534862395639415 2116 58-59 -0.2974200821370516 2116 60-61 -0.7271725684752184 2116 62-63 -0.39366560459610156 2116 64-65 -0.6853630121305301 2116 66-67 -0.8438223577802617 2116 68-69 -0.37312246728412646 2116 70-71 -0.5228423139164349 2116 72-73 -0.44708523832467506 2116 74-75 -0.40211265214790615 2116 76 -0.30607264238898324 2117 1 0.36447330955761714 2117 2 0.05200571992089564 2117 3 0.6653491139224954 2117 4 0.3880552120891956 2117 5 0.22509602781563132 2117 6 0.8347236727409211 2117 7 0.7691288636509412 2117 8 0.24627805462160524 2117 9 0.32922356546773557 2117 10-11 0.5822364228732368 2117 12-13 0.44320153404103735 2117 14-15 0.6215512851844096 2117 16-17 0.2709988908794969 2117 18-19 0.17905235994360424 2117 20-21 -0.05140903839111388 2117 22-23 0.4313350121132373 2117 24-25 0.08430639335112033 2117 26-27 0.41394489405823265 2117 28-29 0.29384810482103063 2117 30-31 0.42392043296084125 2117 32-33 0.12525777470223431 2117 34-35 0.4623992242574886 2117 36-37 0.34960995248900417 2117 38-39 0.6157221278589446 2117 40-41 -0.04762953986836749 2117 42-43 0.22082479059470472 2117 44-45 0.30239446647877344 2117 46-47 0.002863831888724633 2117 48-49 0.20446505422292205 2117 50-51 0.22497638355088867 2117 52-53 0.13298040396571054 2117 54-55 0.1197369210814827 2117 56-57 -0.0737766288241204 2117 58-59 0.25470969784009156 2117 60-61 0.30156528559317763 2117 62-63 0.23725271804107706 2117 64-65 0.06824318046893296 2117 66-67 0.42967266356909306 2117 68-69 0.11974246909739605 2117 70-71 0.0617582342076588 2117 72-73 -0.9824791917770881 2117 74-75 0.01412445018291919 2117 76 -0.442944726721052 2118 1 0.24103007292958623 2118 2 -0.03588842016981175 2118 3 0.4493819409327884 2118 4 -0.7087970663644789 2118 5 -0.052867221770434014 2118 6 0.020104494855957 2118 7 0.2612370632046108 2118 8 -0.17099751949113084 2118 9 -0.1028502593108982 2118 10-11 -0.9241571053104849 2118 12-13 0.028735782835219936 2118 14-15 0.0029898065291575904 2118 16-17 0.09055606818854756 2118 18-19 -0.1379132112895718 2118 20-21 0.10574982346188477 2118 22-23 0.08798770090454866 2118 24-25 0.2394900554344872 2118 26-27 -0.002577044607093626 2118 28-29 -0.024649483545537976 2118 30-31 -0.26852656509545625 2118 32-33 -0.3339041826802429 2118 34-35 -0.4390612537498555 2118 36-37 -0.3119312432492478 2118 38-39 -0.1534539573761009 2118 40-41 -0.27533092925717995 2118 42-43 -0.11612899243027641 2118 44-45 -0.42800201325631093 2118 46-47 -0.46122712628562823 2118 48-49 -0.24429223466916739 2118 50-51 -0.13225581715459356 2118 52-53 -0.2841601906639042 2118 54-55 -0.614855170920535 2118 56-57 -0.14125962140924742 2118 58-59 -0.304395568159471 2118 60-61 -0.25801015893924983 2118 62-63 -0.08913442111376924 2118 64-65 -0.3065138564614571 2118 66-67 -0.2994595083407816 2118 68-69 -0.34039134056353504 2118 70-71 -0.2537473089497837 2118 72-73 -0.11826180282021426 2118 74-75 -0.2348384574551261 2118 76 -0.23660159087770083 2119 1 0.24753659767989333 2119 2 0.1380244252045415 2119 3 -0.12341094380877138 2119 4 0.01093939204479355 2119 5 0.6432990317835525 2119 6 0.30239107355392036 2119 7 0.1960533495646004 2119 8 0.5501969836801166 2119 9 0.2835726101211904 2119 10-11 0.30509765156055124 2119 12-13 0.2900834718342722 2119 14-15 0.36382399038861024 2119 16-17 0.500829360453622 2119 18-19 -0.05102969084049391 2119 20-21 -0.19418137505090982 2119 22-23 -0.1756951379086047 2119 24-25 0.044141673864167785 2119 26-27 0.17059083102169836 2119 28-29 0.16739323716598875 2119 30-31 0.009082879389488596 2119 32-33 -0.0720370652347384 2119 34-35 0.05370775550873219 2119 36-37 0.4492250773747912 2119 38-39 -0.3681237102375121 2119 40-41 0.03173329761458632 2119 42-43 -0.17460570016520194 2119 44-45 -0.04234461732426098 2119 46-47 -0.41454691195305315 2119 48-49 -0.12193949719012664 2119 50-51 -0.23401367237075021 2119 52-53 -0.28397793506865554 2119 54-55 0.17398577462148523 2119 56-57 -0.0017361286630190875 2119 58-59 -0.12281659681004697 2119 60-61 -0.15077552308593312 2119 62-63 -0.11830215093807794 2119 64-65 -0.39656634607288055 2119 66-67 -0.20125108084205579 2119 68-69 -0.38110496234672553 2119 70-71 -0.2368841099995791 2119 72-73 -0.091572749091263 2119 74-75 -0.16157854275074612 2119 76 -0.1750857710920357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 31.0 13 349.0 14 1123.0 15 1994.0 16 2603.0 17 3016.0 18 3186.0 19 2790.0 20 2197.0 21 1744.0 22 1319.0 23 1172.0 24 1071.0 25 1097.0 26 1375.0 27 1617.0 28 1932.0 29 2316.0 30 2882.0 31 3699.0 32 4711.0 33 6434.0 34 8804.0 35 12988.0 36 24504.0 37 87254.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.70824712280952 19.372808148883973 13.213946621736577 27.704998106569928 2 21.419907907951856 19.432080742444118 32.39521648217158 26.752794867432456 3 19.7097838196796 22.373757608021556 27.397109912243632 30.51934866005521 4 16.165502252907377 17.923922528524937 30.405742855731603 35.50483236283608 5 17.11496139049114 31.528629211509863 31.396363516621022 19.960045881377976 6 30.585755917655 32.68060304375744 17.095752679615167 19.63788835897239 7 29.172543617494195 27.336190857751262 19.685086905696206 23.806178619058336 8 25.242441372270303 32.155382006377295 18.623668424721064 23.97850819663134 9 24.078393493186397 17.454681162840473 19.974864029768014 38.49206131420512 10-11 27.622400649803247 22.79305632542849 23.70354922094957 25.88099380381869 12-13 26.100521928115516 20.559357660708308 23.512834162966705 29.82728624820947 14-15 24.973039128251425 21.61617048600916 21.811002230960685 31.59978815477873 16-17 25.782206147885123 23.286994605096346 22.733234911557606 28.197564335460928 18-19 25.11813357188723 22.84327338386139 23.545763381611227 28.49282966264016 20-21 24.91040508427136 23.224154679516378 23.755961560625437 28.109478675586825 22-23 26.4369487786004 22.91269915317026 22.766987360668246 27.8833647075611 24-25 25.73665406209353 23.359987706425038 22.463764138983255 28.439594092498176 26-27 24.712555362248846 23.763919455131198 23.62836083837791 27.89516434424205 28-29 25.382939371820274 23.05676448474005 24.583856999379833 26.976439144059842 30-31 25.186187290419245 22.636916947022375 24.21559857087191 27.96129719168647 32-33 24.50537569494372 23.59213869786893 24.184315813159614 27.718169794027737 34-35 24.994923412125637 23.385233440719176 24.450493663869512 27.169349483285675 36-37 25.154081302240833 22.463215318672514 24.617060628179726 27.765642750906927 38-39 24.2337096411264 22.67012057582227 26.060183635275973 27.03598614777536 40-41 24.17251617647866 21.92893874616512 26.33925876328831 27.55928631406791 42-43 23.333644331509422 22.103463604981094 26.313189798528065 28.249702264981423 44-45 22.35043274481502 22.329577573006823 27.350734595985926 27.96925508619223 46-47 22.64377720090665 22.786196071544214 27.343051111635543 27.226975615913595 48-49 22.456080654632867 23.03481167231037 27.124346217804828 27.384761455251937 50-51 22.084254894105122 23.74690602549819 26.363681267116334 27.805157813280353 52-53 21.4223775993502 23.561679170622746 26.88506056232129 28.130882667705766 54-55 21.499486853009454 23.38660549149603 26.598850770269305 28.51505688522521 56-57 21.634496649452004 23.62863524853328 26.296450779050428 28.44041732296429 58-59 21.997815695163247 23.41871147967444 25.87248708900219 28.710985736160122 60-61 21.951714789060915 22.74503454823856 26.061006865742087 29.24224379695844 62-63 21.74014455926985 22.988710766208037 26.09366167423124 29.177483000290877 64-65 22.319424397258096 23.02163998485256 25.268784747185926 29.390150870703422 66-67 22.261249444319436 23.510913291879106 24.23206318019417 29.995774083607284 68-69 22.119653804147983 24.139586957834137 24.26060183635276 29.480157401665117 70-71 22.613105316162528 24.09272893416059 24.563892463853225 28.73027328582366 72-73 22.61688500568029 23.838010197081374 24.08223523536159 29.462869561876747 74-75 22.50504917456972 24.564234281700035 23.94708245521602 28.983634088514226 76 22.67902616789603 24.576857218124342 23.39139884088514 29.352717773094483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 2.5 15 3.0 16 5.0 17 9.0 18 16.5 19 22.0 20 28.5 21 37.0 22 55.0 23 98.0 24 139.0 25 155.0 26 273.0 27 487.5 28 683.0 29 782.0 30 911.5 31 1196.5 32 1715.0 33 2078.0 34 2360.0 35 3047.0 36 3651.5 37 3851.0 38 4505.0 39 5828.0 40 7010.0 41 7872.5 42 8222.0 43 8439.0 44 8978.0 45 9402.5 46 9505.0 47 9535.5 48 9240.5 49 8406.5 50 7898.0 51 7349.5 52 6222.0 53 5238.0 54 4833.0 55 4629.5 56 4064.0 57 3662.5 58 3623.0 59 3597.0 60 3663.0 61 3901.5 62 4048.0 63 4134.5 64 4260.0 65 4398.0 66 4487.5 67 4478.0 68 4457.0 69 4454.0 70 4272.0 71 4072.0 72 3941.5 73 3451.0 74 2809.0 75 2527.0 76 2157.0 77 1432.0 78 832.5 79 588.0 80 434.0 81 200.5 82 82.5 83 44.0 84 32.0 85 16.5 86 8.0 87 3.0 88 6.5 89 10.5 90 7.0 91 4.5 92 6.0 93 6.0 94 7.0 95 6.5 96 5.0 97 4.0 98 6.0 99 28.0 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.7441015537102995E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 2.7441015537102995E-4 72-73 0.0 74-75 5.488203107420599E-4 76 5.488203107420599E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 182209.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.792537222490324 #Duplication Level Percentage of deduplicated Percentage of total 1 72.13874079916211 40.24803381220833 2 14.32894851482823 15.98896786745403 3 5.385333272360341 9.013842211610399 4 2.4779976344105723 5.5301510102037925 5 1.358995261475272 3.791089685552354 6 0.8849968239184705 2.9625730944154194 7 0.7149974310746996 2.79240645510541 8 0.49999820346597007 2.231693470244274 9 0.4049985448047356 2.0336306747465365 >10 1.802993521685767 15.350703534968622 >50 0.001999992813849987 0.05690818349083366 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.01207404683632532 0.0 2 0.0 0.0 0.0 0.0164646093222618 0.0 3 0.0 0.0 0.0 0.02030635149745622 0.0 4 0.0 0.0 0.0 0.07244428101795192 0.0 5 0.0 0.0 0.0 0.08232304661130899 0.0 6 0.0 0.0 0.0 0.08287186692205105 0.0 7 0.0 0.0 0.0 0.08451832785427724 0.0 8 0.0 0.0 0.0 0.08561596847576135 0.0 9 0.0 0.0 0.0 0.09275063251540813 0.0 10 0.0 0.0 0.0 0.0971411950013446 0.0 11 0.0 0.0 0.0 0.09823883562282873 0.0 12 0.0 0.0 0.0 0.0998852965550549 0.0 13 0.0 0.0 0.0 0.0998852965550549 0.0 14 0.0 0.0 0.0 0.10427585904099139 0.0 15 0.0 0.0 0.0 0.10537349966247551 0.0 16 0.0 0.0 0.0 0.10647114028395963 0.0 17 0.0 0.0 0.0 0.10866642152692788 0.0 18 0.0 0.0 0.0 0.11360580432360641 0.0 19 0.0 0.0 0.0 0.11744754649880083 0.0 20 0.0 0.0 0.0 0.1218381089847373 0.0 21 0.0 0.0 0.0 0.1218381089847373 0.0 22 0.0 0.0 0.0 0.12567985115993172 0.0 23 0.0 0.0 0.0 0.12842395271364201 0.0 24 0.0 0.0 0.0 0.13391215582106264 0.0 25 0.0 0.0 0.0 0.13775389799625704 0.0 26 0.0 0.0 0.0 0.15147440576480856 0.0 27 0.0 0.0 0.0 0.2370903742405699 0.0 28 0.0 0.0 0.0 0.3051440927725853 0.0 29 0.0 0.0 0.0 0.41161523305654496 0.0 30 0.0 0.0 0.0 0.5257698576908935 0.0 31 0.0 0.0 0.0 0.6432174041896942 0.0 32 0.0 0.0 0.0 0.7859106849826298 0.0 33 0.0 0.0 0.0 0.96976548908122 0.0 34 0.0 0.0 0.0 1.2392362616555714 0.0 35 0.0 0.0 0.0 1.6256057604179814 0.0 36 0.0 0.0 0.0 2.123934602571772 0.0 37 0.0 0.0 0.0 2.7880071785696643 0.0 38 0.0 0.0 0.0 3.5327563402466398 0.0 39 0.0 0.0 0.0 4.20670768183789 0.0 40 0.0 0.0 0.0 4.9492615622718965 0.0 41 0.0 0.0 0.0 5.7022430286100025 0.0 42 0.0 0.0 0.0 6.531510518141255 0.0 43 0.0 0.0 0.0 7.36407092953696 0.0 44 0.0 0.0 0.0 8.156018637937752 0.0 45 0.0 0.0 0.0 8.92217179173367 0.0 46 0.0 0.0 0.0 9.720156523552623 0.0 47 0.0 0.0 0.0 10.511006591331933 0.0 48 0.0 0.0 0.0 11.23544940151145 0.0 49 0.0 0.0 0.0 11.872080961972241 0.0 50 0.0 0.0 0.0 12.58829146749063 0.0 51 0.0 0.0 0.0 13.26004752783891 0.0 52 0.0 0.0 0.0 14.018517197284437 0.0 53 0.0 0.0 0.0 14.703444945090528 0.0 54 0.0 0.0 0.0 15.39166561476107 0.0 55 0.0 0.0 0.0 16.03598065957225 0.0 56 0.0 0.0 0.0 16.69401621215198 0.0 57 0.0 0.0 0.0 17.3597352490821 0.0 58 0.0 0.0 0.0 17.961242309655397 0.0 59 0.0 0.0 0.0 18.58525100296912 0.0 60 0.0 0.0 0.0 19.186209243231673 0.0 61 0.0 0.0 0.0 19.8376589520825 0.0 62 0.0 0.0 0.0 20.45892354384251 0.0 63 0.0 0.0 0.0 21.10323858865369 0.0 64 0.0 0.0 0.0 21.751944195950802 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTACAG 25 2.3856823E-4 55.999996 3 TATGCAT 20 0.0069426987 52.5 4 ATAGTGC 20 0.0069426987 52.5 26 AGACTGG 50 1.704851E-4 35.0 51 GGGAATC 40 0.002414112 35.0 29 AACGCTC 60 1.2109469E-5 35.0 7 CGATGTA 50 1.704851E-4 35.0 64 ATAACGC 60 1.2109469E-5 35.0 5 TGGGAAT 40 0.002414112 35.0 28 ACTGGGG 40 0.002414112 35.0 53 CATAACG 65 2.0943779E-5 32.307693 4 TGTAACA 55 2.9836487E-4 31.81818 67 GTAACAC 55 2.9836487E-4 31.81818 68 CTGTAAG 45 0.004299045 31.111109 4 TTCCGGA 45 0.004299045 31.111109 38 GAGAGGT 45 0.004299045 31.111109 6 TACAGGG 45 0.004299045 31.111109 5 GTGTATG 80 2.4535893E-6 30.625 1 AGTACCG 70 3.474913E-5 30.0 5 ATGTAAC 60 4.9680436E-4 29.166666 66 >>END_MODULE