FastQCFastQC Report
Wed 25 May 2016
SRR1780186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182209
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT88054.832362836083838No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC28731.5767607527619383No Hit
CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG24041.319364027023912RNA PCR Primer, Index 15 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG22781.2502126678704124No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5890.3232551630270733No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT3090.16958547601929652RNA PCR Primer, Index 48 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC252.8314025E-670.03
TAAGTAG150.002221892670.066
TGTGACT150.002221892670.043
CCGAAAT150.002221892670.047
CTTAATA252.3856823E-455.9999962
GACTCAG252.3856823E-455.99999631
CTGTAAG200.006942698752.563
ATATGTA200.006942698752.521
AATTCAC200.006942698752.558
GTAAGTA200.006942698752.565
GAATTCA200.006942698752.557
AATATCG352.0805E-550.036
ATCGTAT352.0805E-550.039
TCTTTAT751.4551915E-1146.6666681
TTGATAA305.866315E-446.66666429
GATAATT305.866315E-446.66666431
GGTAACC305.866315E-446.66666419
CTTTATA802.910383E-1143.752
ATATCGT459.171449E-538.88888537
CCTCTGG459.171449E-538.88888524
Produced by FastQC (version 0.11.5)