FastQCFastQC Report
Wed 25 May 2016
SRR1780185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433624
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAC300.00577235529.3006613
TAAGACT300.00577235529.3006619
TCAGATG703.6185156E-828.254214
TGACAAT450.00138801324.47083535
CTATCGG450.001404922524.42003442
CTGTACC651.823961E-523.668652
TAGATCT904.0272243E-722.0237539
CGACGAA1004.5991328E-822.00340522
AATTATC602.8836096E-421.98309930
CTAGATA602.8881128E-421.9780343
GTACAGA500.002592791321.9780332
GACTTTA500.002592791321.9780333
TCTGTAC602.8881128E-421.978031
TGACGCT2650.021.60071813
TATCGGG654.9584405E-420.28741543
CGGCAAA550.004451161720.02159134
GTACATG550.00450502619.9800281
CTGCCGA2550.019.87374518
TCTGACG3100.019.8717511
CAGATGT1352.6211637E-919.5337755
Produced by FastQC (version 0.11.5)