SRR1780185_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780185_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	433624
Sequences flagged as poor quality	0
Sequence length	50
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT	8647	1.994123941479254	No Hit
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG	4406	1.0160876704241464	RNA PCR Primer, Index 13 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG	2049	0.4725291958009704	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC	1672	0.385587513606258	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC	887	0.20455509842628636	No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT	482	0.11115620906591887	TruSeq Adapter, Index 8 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTCTG	535	0.0	36.601105	44
CGTCTTC	535	0.0	35.778606	42
GTCTTCT	565	0.0	34.268272	43
CCGTCTT	565	0.0	33.87886	41
GTATACG	40	1.8163842E-5	33.00268	44
CTAGATT	40	1.8178183E-5	32.99888	33
CATCGTT	35	3.215129E-4	31.4275	32
GCCGTCT	640	0.0	29.905231	40
TCGACAA	30	0.005738724	29.335718	41
TGTATAC	45	4.061345E-5	29.335716	43
TACAGCA	30	0.00574196	29.332333	2
CTTTATA	125	0.0	28.15904	2
TGCCGTC	705	0.0	28.084148	39
TCGTTGT	40	7.0262095E-4	27.499063	34
TTGTCTA	50	8.331254E-5	26.399101	29
ATTAACG	50	8.331254E-5	26.399101	18
AATACTG	50	8.331254E-5	26.399101	5
TTCCTTT	60	9.736741E-6	25.66579	21
GTAAGGT	70	1.1298052E-6	25.142	19
ATGCCGT	1525	0.0	24.959406	44