FastQCFastQC Report
Wed 25 May 2016
SRR1780184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116995
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG150.002219897570.04
CCGTACC200.00693651152.555
ATTCAGA200.00693651152.519
TGCTATT200.00693651152.510
GTCTATT450.00429280731.1111091
TAGATCT450.00429280731.11110939
TATAACC450.00429280731.1111093
TTTTGAG604.957292E-429.16666650
GCTCTAA500.007184330427.9999981
AATGATG500.007184330427.99999824
AGTGTTG500.007184330427.99999826
CGGACGC500.007184330427.99999832
TGTAGAT750.001823249723.33333437
TACCCCT952.7607678E-422.1052655
CCCAACA1003.9045687E-421.034
TAAGAGA850.003770739820.5882364
ATAATAT900.005249132419.4444433
ACGAAGT1107.4223534E-419.0909170
GATGCAC3551.8189894E-1216.76056570
AGAGGGA1400.00372713515.0000016
Produced by FastQC (version 0.11.5)