##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780184_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116995 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.384512158639257 34.0 31.0 34.0 12.0 34.0 2 30.437369118338392 34.0 31.0 34.0 12.0 34.0 3 30.358126415658788 34.0 31.0 34.0 11.0 34.0 4 30.14905765203641 34.0 31.0 34.0 11.0 34.0 5 30.240129920082055 34.0 31.0 34.0 11.0 34.0 6 33.035787854181805 37.0 34.0 38.0 21.0 38.0 7 33.41990683362537 37.0 34.0 38.0 21.0 38.0 8 33.338416171631266 37.0 34.0 38.0 21.0 38.0 9 33.864293345869484 38.0 34.0 38.0 21.0 38.0 10-11 33.56487884097611 37.5 34.0 38.0 21.0 38.0 12-13 33.613556134877555 38.0 34.0 38.0 21.0 38.0 14-15 33.6531518440959 38.0 34.0 38.0 21.0 38.0 16-17 33.768225137826406 38.0 34.0 38.0 21.0 38.0 18-19 33.73830505577162 38.0 34.0 38.0 21.0 38.0 20-21 33.750681653062095 38.0 34.0 38.0 21.0 38.0 22-23 33.77884952348391 38.0 34.0 38.0 21.0 38.0 24-25 33.86974657036626 38.0 34.0 38.0 21.5 38.0 26-27 33.87070387623403 38.0 34.0 38.0 22.0 38.0 28-29 34.0090773109962 38.0 34.0 38.0 22.0 38.0 30-31 34.0407666994316 38.0 34.0 38.0 22.5 38.0 32-33 34.11966323347151 38.0 34.0 38.0 23.0 38.0 34-35 34.04098038377708 38.0 34.0 38.0 22.5 38.0 36-37 33.95330997051156 38.0 34.0 38.0 22.0 38.0 38-39 33.98485832727894 38.0 34.0 38.0 22.0 38.0 40-41 34.05235266464379 38.0 34.0 38.0 22.5 38.0 42-43 34.10891063720672 38.0 34.0 38.0 23.0 38.0 44-45 34.137223812983464 38.0 34.0 38.0 23.0 38.0 46-47 34.09220479507671 38.0 34.5 38.0 22.5 38.0 48-49 34.071015855378434 38.0 34.0 38.0 23.0 38.0 50-51 33.96229326039574 38.0 34.0 38.0 22.0 38.0 52-53 33.993456985341254 38.0 34.0 38.0 22.5 38.0 54-55 33.97539638446088 38.0 34.0 38.0 22.5 38.0 56-57 33.91090644899354 38.0 34.0 38.0 22.0 38.0 58-59 33.98812769776486 38.0 34.0 38.0 23.0 38.0 60-61 33.99546561818881 38.0 34.0 38.0 23.0 38.0 62-63 33.845006196846015 38.0 34.0 38.0 22.0 38.0 64-65 33.81668874738237 38.0 34.0 38.0 22.0 38.0 66-67 33.65594256164793 37.0 34.0 38.0 22.0 38.0 68-69 33.55756228898671 37.0 34.0 38.0 22.0 38.0 70-71 33.5610410701312 37.0 34.0 38.0 22.0 38.0 72-73 33.63498440104278 37.0 34.0 38.0 22.0 38.0 74-75 33.68069148254199 37.0 34.0 38.0 22.0 38.0 76 31.76010940638489 36.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14613271101466907 1101 2 0.2756593967307026 1101 3 0.1623993245700035 1101 4 0.8974823551872291 1101 5 -0.47919813988714566 1101 6 -0.17008578516436756 1101 7 -0.22255922879972445 1101 8 -0.0397618342186874 1101 9 -0.06163832122508239 1101 10-11 0.3212442480390223 1101 12-13 0.4745908303634607 1101 14-15 0.30811264434372987 1101 16-17 0.18439791719266196 1101 18-19 0.45440593195603896 1101 20-21 0.49394702330191365 1101 22-23 0.2360979286654441 1101 24-25 0.07417048890837208 1101 26-27 0.10792824708863691 1101 28-29 -0.0930352187544159 1101 30-31 -0.35035907994856075 1101 32-33 -0.06571998669062395 1101 34-35 -0.490587155014353 1101 36-37 -0.28310291464577375 1101 38-39 0.2641344499562237 1101 40-41 0.14078424039591653 1101 42-43 -0.2800391152582762 1101 44-45 0.2881092319514309 1101 46-47 -0.0690426955230663 1101 48-49 -0.21102832947222083 1101 50-51 -0.05749175646674587 1101 52-53 0.004190123889678432 1101 54-55 -0.07282264601893473 1101 56-57 0.38880699296792187 1101 58-59 0.562521263848275 1101 60-61 0.3163945843527145 1101 62-63 0.1914964596626305 1101 64-65 0.8132514902285877 1101 66-67 0.27291376523108823 1101 68-69 0.4875665056659244 1101 70-71 0.20597100787900047 1101 72-73 0.6471915155636054 1101 74-75 -0.18607534503986756 1101 76 -0.41743001591577666 1102 1 0.41707358623418145 1102 2 0.5290788496182408 1102 3 0.5617641441475882 1102 4 0.8907993219084496 1102 5 0.12007315619266024 1102 6 0.46604935628992195 1102 7 -0.2066894602703826 1102 8 0.6172309956464872 1102 9 0.4982264593843766 1102 10-11 0.12709531186881406 1102 12-13 0.7295836212605096 1102 14-15 0.2178624689871711 1102 16-17 0.3671535480865913 1102 18-19 0.43559894107458064 1102 20-21 0.3789813152510959 1102 22-23 0.1276691234281344 1102 24-25 0.25007260098070105 1102 26-27 0.03558392474431571 1102 28-29 0.42019445936887934 1102 30-31 0.114199411205675 1102 32-33 0.8700897709276489 1102 34-35 0.3311093222672312 1102 36-37 0.649233808914353 1102 38-39 0.20896532829774372 1102 40-41 0.663755949537844 1102 42-43 0.4382408317450768 1102 44-45 0.5747538816279913 1102 46-47 0.19271106490090517 1102 48-49 0.23167878610723136 1102 50-51 0.4311977455206204 1102 52-53 0.898523184446141 1102 54-55 0.1858867297117186 1102 56-57 0.38285461201553517 1102 58-59 0.5877238980934649 1102 60-61 0.45396667538224733 1102 62-63 0.4483645915307619 1102 64-65 0.4746818152227448 1102 66-67 0.4030819126864671 1102 68-69 0.7264167511651038 1102 70-71 0.4958207857606851 1102 72-73 0.8388318787465181 1102 74-75 0.532326377351211 1102 76 0.5884444287884527 1103 1 0.7023838278341934 1103 2 0.8832877955222429 1103 3 0.5442065908183586 1103 4 0.8511387189644388 1103 5 0.1689349455571154 1103 6 -0.00525892018644214 1103 7 0.16684932337317804 1103 8 0.22180955008683867 1103 9 0.5144616865214502 1103 10-11 0.4417125259846415 1103 12-13 0.7682570709707832 1103 14-15 0.6297487122033658 1103 16-17 0.5566043235758045 1103 18-19 0.5992322159439567 1103 20-21 0.910216680130489 1103 22-23 0.8701323232738645 1103 24-25 1.1481111504623271 1103 26-27 0.7947552669972282 1103 28-29 0.6896551523045318 1103 30-31 0.6510546624017408 1103 32-33 1.255809566334392 1103 34-35 0.7545567368440373 1103 36-37 0.9766335095543965 1103 38-39 0.5856446366476789 1103 40-41 0.6806982538748869 1103 42-43 0.6693953878016075 1103 44-45 0.4244724281711214 1103 46-47 0.582765151715293 1103 48-49 0.94480287356442 1103 50-51 0.07877944966850237 1103 52-53 1.0127161522419854 1103 54-55 0.8846488854707246 1103 56-57 1.0276539214674116 1103 58-59 0.3350539929519982 1103 60-61 0.5596793081887981 1103 62-63 0.5387832363300689 1103 64-65 0.8070494219041677 1103 66-67 0.833457765540949 1103 68-69 1.3972080348316211 1103 70-71 0.5962946193128786 1103 72-73 0.6142154910845576 1103 74-75 0.6262987737919516 1103 76 0.4838888314000478 1104 1 0.2463836873156815 1104 2 0.06635707114930867 1104 3 -0.3556388388169971 1104 4 0.7455741858371994 1104 5 -0.5694634946873869 1104 6 0.2813900645672973 1104 7 0.05874667459919891 1104 8 -0.1389717328471889 1104 9 0.09794724824420342 1104 10-11 0.18461634106228075 1104 12-13 -0.0719953354087508 1104 14-15 -0.15275497640760705 1104 16-17 -0.17118496891347235 1104 18-19 0.24510360637464856 1104 20-21 -0.7945890530248576 1104 22-23 -0.3885666509409873 1104 24-25 -0.10710559934446451 1104 26-27 0.030185253647964316 1104 28-29 -0.16138644117540935 1104 30-31 -0.10166349259017693 1104 32-33 0.05409963167550558 1104 34-35 0.22415643472923819 1104 36-37 0.06934938112048172 1104 38-39 -0.06398123281062595 1104 40-41 -0.3404322175647252 1104 42-43 0.3425756730541849 1104 44-45 0.13631286939925502 1104 46-47 0.34113917686810424 1104 48-49 -0.09802892577514655 1104 50-51 -0.08734417149358364 1104 52-53 0.009668656984480606 1104 54-55 -0.16316253868441066 1104 56-57 0.02446590769660162 1104 58-59 0.1167848297397498 1104 60-61 0.0322562420748298 1104 62-63 -0.5088613220905032 1104 64-65 -0.28156067435101306 1104 66-67 -0.03977257943200385 1104 68-69 -0.21838161597987238 1104 70-71 -0.05889929623549506 1104 72-73 0.10377320906390253 1104 74-75 0.004816127828348726 1104 76 -0.4684139157368854 1105 1 0.3615283271341738 1105 2 0.7268789089258263 1105 3 0.22001345840364905 1105 4 1.2568475834548956 1105 5 1.2585285643029707 1105 6 0.87611496530468 1105 7 0.5926377693240994 1105 8 0.48595736528100275 1105 9 0.63637606276491 1105 10-11 0.7450247358438986 1105 12-13 -0.11081411022441046 1105 14-15 0.6167430383399832 1105 16-17 0.3782690866735905 1105 18-19 0.8019669075657916 1105 20-21 0.6216049344964176 1105 22-23 0.4367311369206064 1105 24-25 0.4105135158001758 1105 26-27 0.7470386285770658 1105 28-29 0.5159391402199418 1105 30-31 0.5247190939101074 1105 32-33 0.5527524961111254 1105 34-35 0.8384857030219166 1105 36-37 0.4436403624023839 1105 38-39 0.9411591528955583 1105 40-41 0.6277682929287494 1105 42-43 0.9565149810519245 1105 44-45 0.8967708954911444 1105 46-47 0.912820969267429 1105 48-49 0.953410382222593 1105 50-51 0.70810392833625 1105 52-53 0.6724593546589048 1105 54-55 0.7258405809979465 1105 56-57 0.6581159360573494 1105 58-59 0.5620131337669818 1105 60-61 0.4494074048655321 1105 62-63 0.5894795741091627 1105 64-65 0.44950852587587065 1105 66-67 1.0952245844931312 1105 68-69 0.9435937101687415 1105 70-71 1.0891792630384742 1105 72-73 0.9864660621552233 1105 74-75 0.2798799395129734 1105 76 0.555834524421936 1106 1 0.18806581030041514 1106 2 0.221211543969968 1106 3 0.39525287184072866 1106 4 0.8515094119750231 1106 5 -0.08471174693449512 1106 6 0.7866154890215711 1106 7 -0.0700698470158656 1106 8 0.25780384141447144 1106 9 0.5839295567047529 1106 10-11 0.37415835846847045 1106 12-13 0.6745180759195364 1106 14-15 0.8098823470336853 1106 16-17 0.6445697749872252 1106 18-19 0.7531022509744432 1106 20-21 0.3797225463473808 1106 22-23 0.359623176423824 1106 24-25 0.7757592886566798 1106 26-27 0.842551497049314 1106 28-29 0.6739146003426413 1106 30-31 0.2597557540926587 1106 32-33 0.7036484260826938 1106 34-35 0.5067484592691187 1106 36-37 0.6774425470499992 1106 38-39 0.23497368229852356 1106 40-41 0.5275232682392428 1106 42-43 0.5118045216773908 1106 44-45 0.7399831504664505 1106 46-47 0.41537408912377316 1106 48-49 0.534390106897142 1106 50-51 0.44185935254693476 1106 52-53 0.6456188638613582 1106 54-55 0.7727412992382625 1106 56-57 0.6814961545221081 1106 58-59 0.8521429407398955 1106 60-61 0.6257877729636974 1106 62-63 0.5046748456937777 1106 64-65 0.9738520093413783 1106 66-67 0.7023714496734357 1106 68-69 0.9616993513478462 1106 70-71 0.6380439196298227 1106 72-73 0.9240221746795356 1106 74-75 0.583224245962171 1106 76 1.0114869260379713 1107 1 0.2253613862858792 1107 2 0.005498106408122538 1107 3 0.18384830569640442 1107 4 0.5395795053488932 1107 5 0.24922129014518646 1107 6 -0.4196346234228798 1107 7 -0.14261900773047387 1107 8 -0.2341091535660027 1107 9 0.2672679875199364 1107 10-11 0.0899392627017761 1107 12-13 0.7671363225057277 1107 14-15 -0.01672380952498287 1107 16-17 0.29310439888301687 1107 18-19 0.22438393782113764 1107 20-21 1.07849285381549 1107 22-23 0.5221908679841931 1107 24-25 0.4157751208355833 1107 26-27 0.21069913211758262 1107 28-29 0.5027426592815232 1107 30-31 0.6162361527757128 1107 32-33 0.5740179635311975 1107 34-35 0.21224294499316443 1107 36-37 0.6484003938736649 1107 38-39 0.16962546431820158 1107 40-41 0.532223446351253 1107 42-43 0.3592758708309063 1107 44-45 0.28895046061524 1107 46-47 0.4688031951453979 1107 48-49 0.32043080279336067 1107 50-51 0.43441552979537335 1107 52-53 0.17502533706359458 1107 54-55 0.0047771735163664175 1107 56-57 0.2232400330461175 1107 58-59 0.22320063823732994 1107 60-61 0.11509655442564792 1107 62-63 0.3767265521507923 1107 64-65 0.4881189614670305 1107 66-67 0.37148790614394045 1107 68-69 0.4322568959208297 1107 70-71 0.24814053190335983 1107 72-73 0.16995168550119644 1107 74-75 0.6983943519298279 1107 76 1.316751125523428 1108 1 -0.8006139367033249 1108 2 -0.19763781689158932 1108 3 -0.1675403436049585 1108 4 0.43378068018524374 1108 5 0.6200119895087397 1108 6 0.27103114743863443 1108 7 -0.119766897292223 1108 8 -0.16712350763299355 1108 9 -0.012985864290897098 1108 10-11 0.15212660098019626 1108 12-13 0.010070508064494277 1108 14-15 0.08008216089529441 1108 16-17 -0.2679071951356704 1108 18-19 0.014583950531893208 1108 20-21 0.07088002322253573 1108 22-23 0.24758875981572004 1108 24-25 -0.10364962699223668 1108 26-27 -0.3308468580951427 1108 28-29 -0.00882866163764362 1108 30-31 0.039594877342864265 1108 32-33 -0.17641102423924337 1108 34-35 -0.09453551573515995 1108 36-37 -0.0461466949109095 1108 38-39 0.20109364659034412 1108 40-41 0.18132960834066836 1108 42-43 0.424196020091415 1108 44-45 -0.08552635588694812 1108 46-47 -0.2804273505996733 1108 48-49 -0.06904015768528637 1108 50-51 -0.2392314563953022 1108 52-53 -0.4680205215020905 1108 54-55 -0.25953376771457926 1108 56-57 -0.2650887758659266 1108 58-59 0.08277410182557787 1108 60-61 0.1289739683330211 1108 62-63 -0.06645186136618264 1108 64-65 -0.0037733966492368154 1108 66-67 0.32455800592331485 1108 68-69 0.12085717010984354 1108 70-71 -0.2093510926211195 1108 72-73 -0.7260471523732122 1108 74-75 -0.18381786309972625 1108 76 -0.7093149885283054 1109 1 0.8952935710366106 1109 2 0.8506593967307019 1109 3 0.9872390681597487 1109 4 1.264469534674408 1109 5 1.1223242960102873 1109 6 1.7332795994510164 1109 7 1.2389792327387354 1109 8 0.5718567555249052 1109 9 1.1289065505697877 1109 10-11 1.1853067480390251 1109 12-13 1.1941020483121747 1109 14-15 1.4537857212668115 1109 16-17 1.0993818915516371 1109 18-19 1.2450309319560375 1109 20-21 1.2859342027890932 1109 22-23 1.6756411978962191 1109 24-25 0.7598275401904289 1109 26-27 1.122191067601456 1109 28-29 0.940939140219939 1109 30-31 0.949680984154007 1109 32-33 0.6035909107452682 1109 34-35 0.9304064347292353 1109 36-37 0.9916967648414072 1109 38-39 1.0290783602126297 1109 40-41 0.7739573173189953 1109 42-43 1.0146323591007018 1109 44-45 0.5109858345155303 1109 46-47 0.8763499326820607 1109 48-49 0.9709027602713718 1109 50-51 0.9997197819947843 1109 52-53 0.6724593546589048 1109 54-55 0.9903985078272157 1109 56-57 0.7924528263012505 1109 58-59 0.8614795971816136 1109 60-61 0.8103449048655307 1109 62-63 1.093900305816483 1109 64-65 1.025270720997824 1109 66-67 1.188098060102881 1109 68-69 0.7783116979736207 1109 70-71 0.6523652386482297 1109 72-73 0.378882220691807 1109 74-75 1.2378829882934639 1109 76 1.0376180610072971 1110 1 0.9781804757985171 1110 2 0.5316117776830822 1110 3 1.3315843062549853 1110 4 0.2912552489601232 1110 5 0.28408024839124124 1110 6 0.51512483754626 1110 7 1.1556458994053997 1110 8 0.422005565048714 1110 9 0.6405136934269322 1110 10-11 -0.013577180532408306 1110 12-13 0.6255752625978914 1110 14-15 0.34336905460013867 1110 16-17 0.6825664153611584 1110 18-19 0.37107259862271036 1110 20-21 0.46212467897957055 1110 22-23 0.657114412181933 1110 24-25 1.0093811116189926 1110 26-27 0.7275482104585933 1110 28-29 0.7436177116485112 1110 30-31 0.42207681748734416 1110 32-33 0.7781444821738432 1110 34-35 0.5664183394911433 1110 36-37 0.546681883889029 1110 38-39 0.7666527649745376 1110 40-41 0.9037192220809018 1110 42-43 0.7419389067197528 1110 44-45 1.0722209535631535 1110 46-47 0.5948767183963497 1110 48-49 0.729905736461852 1110 50-51 0.8916840677090718 1110 52-53 0.8301974498969997 1110 54-55 0.8786425554462696 1110 56-57 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-0.37865235800408215 2116 28-29 -0.5169328320983979 2116 30-31 -0.23002814214356704 2116 32-33 -0.6142183453100927 2116 34-35 -0.2942259182119358 2116 36-37 -0.6998527593800432 2116 38-39 -0.7083946069153981 2116 40-41 -0.947517596175814 2116 42-43 -0.893050383113831 2116 44-45 -0.7752003679066135 2116 46-47 -0.5829223424044443 2116 48-49 -0.3163539438808769 2116 50-51 -0.05665997579068005 2116 52-53 -0.9058278425729256 2116 54-55 -0.49164085203437224 2116 56-57 -0.3388458328682944 2116 58-59 -0.4272422886315326 2116 60-61 -0.8637413840618038 2116 62-63 -0.3390797979897471 2116 64-65 -1.2061955419779622 2116 66-67 -0.43978364404936343 2116 68-69 -0.32769500617862946 2116 70-71 -0.3697043980299739 2116 72-73 0.027276468096658846 2116 74-75 -0.36939426084148863 2116 76 -0.3971678386466806 2117 1 -1.1358205593981694 2117 2 -0.6247029221098757 2117 3 -0.3512573086518458 2117 4 -0.4012913348908107 2117 5 -1.4273586750042035 2117 6 -0.21300663243303575 2117 7 0.03789227621699354 2117 8 -0.7308153459243698 2117 9 -0.43078547841572146 2117 10-11 -0.3943354621059072 2117 12-13 -1.2648879879197068 2117 14-15 -0.6584425396027527 2117 16-17 -0.8095402098976408 2117 18-19 -0.1026592854352657 2117 20-21 -1.6345368117036543 2117 22-23 -0.699381447031314 2117 24-25 -1.2350547061863892 2117 26-27 -0.5842400918188417 2117 28-29 -0.9804376713742613 2117 30-31 -0.9076333274401946 2117 32-33 -0.8820522051967572 2117 34-35 -0.5792855942562696 2117 36-37 -0.9541592134194588 2117 38-39 -0.7350294296424451 2117 40-41 -1.269611523260707 2117 42-43 -0.8237960829282827 2117 44-45 -1.2950150712815685 2117 46-47 -1.128337567317942 2117 48-49 -1.0796180730619653 2117 50-51 -0.9809867397443384 2117 52-53 -0.7695696308483448 2117 54-55 -0.9171648979698759 2117 56-57 -1.0586567751480302 2117 58-59 -1.2798700404995458 2117 60-61 -1.4364621603518586 2117 62-63 -1.2340888246183042 2117 64-65 -1.6742763804514595 2117 66-67 -1.1124680630855224 2117 68-69 -1.619944642606093 2117 70-71 -0.9427887468590228 2117 72-73 -2.666702018438624 2117 74-75 -1.9496623015616095 2117 76 -1.6430431708767586 2118 1 -1.008276185517694 2118 2 -1.4879173822955138 2118 3 -0.32528434007995344 2118 4 -1.3565510645765286 2118 5 -1.019892464289338 2118 6 -1.2639348200246374 2118 7 -0.8579308796208167 2118 8 -1.0066661845499993 2118 9 -1.6875026254601764 2118 10-11 -2.0200478867924367 2118 12-13 -1.6536683168563613 2118 14-15 -1.3340654023286973 2118 16-17 -0.8854846814820689 2118 18-19 -1.2555659781563193 2118 20-21 -1.2465096174356205 2118 22-23 -0.7165814144633345 2118 24-25 -0.9325998942665024 2118 26-27 -0.8889746627356203 2118 28-29 -1.197544005847476 2118 30-31 -1.3003014596662155 2118 32-33 -0.99272229150192 2118 34-35 -1.6183061120872395 2118 36-37 -1.0846550610012926 2118 38-39 -1.2745791772405468 2118 40-41 -1.0566372519693914 2118 42-43 -0.7092381933337535 2118 44-45 -0.9101225456342732 2118 46-47 -1.1568956197523974 2118 48-49 -1.2509663876687043 2118 50-51 -0.6356032517130785 2118 52-53 -0.7671473869141252 2118 54-55 -1.319725555843192 2118 56-57 -0.9421855163953765 2118 58-59 -1.0690330432678294 2118 60-61 -1.3052742430745425 2118 62-63 -1.3448403308876422 2118 64-65 -1.478217106717537 2118 66-67 -1.254545900571273 2118 68-69 -1.2061276746855611 2118 70-71 -1.4459844804528998 2118 72-73 -1.0889255976602499 2118 74-75 -0.6502607382983001 2118 76 -1.037604317269853 2119 1 -0.364081428963388 2119 2 -0.9767915836614556 2119 3 -1.344010931840252 2119 4 -0.2044030143451998 2119 5 -0.05016344908775139 2119 6 -1.2119409887842743 2119 7 0.06495962489560014 2119 8 -0.06227314643587789 2119 9 -0.16778462590080068 2119 10-11 -0.05767732058842512 2119 12-13 -0.08074476541331421 2119 14-15 -0.40319957285083063 2119 16-17 -0.11599801040915025 2119 18-19 -0.6487803425537706 2119 20-21 -0.7760756011324759 2119 22-23 -0.8191811060253542 2119 24-25 -0.8470964794174165 2119 26-27 -0.5274412853397195 2119 28-29 2.5286571013793946E-4 2119 30-31 -0.5676290648655993 2119 32-33 -0.706029187293943 2119 34-35 -0.8275592515452672 2119 36-37 -0.38866475476643103 2119 38-39 -1.034922865277565 2119 40-41 -0.9499397415045365 2119 42-43 -1.0007781801149847 2119 44-45 -0.8938854890138757 2119 46-47 -0.8209846261414668 2119 48-49 -0.9766768965913784 2119 50-51 -0.7881478650640332 2119 52-53 -0.7231288806352083 2119 54-55 -0.6679961000159196 2119 56-57 -0.8832518305614983 2119 58-59 -0.6850277557595632 2119 60-61 -0.39372985003642924 2119 62-63 -0.21185949810509186 2119 64-65 -0.744949867237473 2119 66-67 -0.8942242438186909 2119 68-69 -1.766449331438146 2119 70-71 -0.28850485939099 2119 72-73 -0.34204302440623024 2119 74-75 -0.7691635803339949 2119 76 -1.0331539978162283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 16.0 13 161.0 14 557.0 15 919.0 16 1194.0 17 1427.0 18 1474.0 19 1382.0 20 1122.0 21 1103.0 22 932.0 23 971.0 24 1083.0 25 1363.0 26 1464.0 27 1805.0 28 2004.0 29 2481.0 30 3015.0 31 3446.0 32 4360.0 33 5380.0 34 7426.0 35 10666.0 36 18697.0 37 42547.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.58459763237745 21.84366853284328 11.562887302876192 27.008846531903075 2 20.437625539552972 20.092311637249455 31.701354758750373 27.7687080644472 3 20.536775075857943 21.487243044574555 27.52681738535835 30.449164494209153 4 16.083593315953674 18.087097739219622 32.1381255609214 33.69118338390529 5 17.402453096286166 31.748365314757045 31.827001153895466 19.02218043506133 6 30.85260053848455 31.867173810846616 17.207572973203984 20.07265267746485 7 29.240565836146843 25.85409632890294 21.614598914483523 23.290738920466687 8 25.46091713321082 30.289328603786487 19.325612205649815 24.924142057352878 9 24.692508226847302 16.39813667250737 20.321381255609214 38.58797384503611 10-11 27.759305953245867 22.585153211675713 23.974956194709176 25.680584640369247 12-13 26.501987264413007 20.432497115261334 23.706141288089235 29.35937433223642 14-15 25.19167485789991 21.48168725159195 21.844095901534253 31.48254198897389 16-17 26.04598487114834 23.448437967434504 22.97534082653105 27.530236334886105 18-19 25.37287918287106 22.700542758237532 23.934783537758022 27.991794521133386 20-21 24.687807171246632 23.491602205222446 24.042907816573358 27.77768280695756 22-23 26.751143211248348 22.791572289414077 23.253130475661354 27.204154023676224 24-25 25.851532116757127 23.417667421684687 22.76208384973717 27.96871661182102 26-27 24.826274627120817 23.705713919398264 23.99760673533057 27.47040471815035 28-29 25.738706782341126 23.280482071883412 24.828838839266634 26.151972306508824 30-31 25.648959357237487 23.042437711013292 24.05487413992051 27.253728791828713 32-33 24.734817727253304 23.87837087055002 24.378819607675542 27.007991794521136 34-35 25.29296123765973 23.44459164921578 24.500619684601908 26.76182742852259 36-37 25.757083636052826 22.764220693192016 24.19718791401342 27.28150775674174 38-39 24.56600709432027 23.077054574981837 25.7869994444207 26.56993888627719 40-41 24.531390230351725 22.67361853070644 25.827172101371854 26.967819137569983 42-43 24.033933074062993 22.536860549596135 25.58613615966494 27.843070216675926 44-45 23.13047566135305 22.539852130432926 26.794734817727257 27.534937390486775 46-47 23.533484336937477 22.975768195222017 26.743023206119922 26.747724261720585 48-49 23.04414718577717 23.05696824650626 26.68661053891192 27.21227402880465 50-51 22.668490106414804 23.68605495961366 26.082311209880764 27.56314372409077 52-53 22.23385614769862 23.59758964058293 26.45925039531604 27.709303816402407 54-55 22.206931920167527 23.49630326082311 26.27462712081713 28.02213769819223 56-57 22.282576178469167 23.48775588700372 26.219069190991068 28.010598743536047 58-59 22.742424889952563 23.396726355827173 25.639984614727123 28.22086413949314 60-61 22.69926065216462 22.926620795760503 25.795119449549127 28.57899910252575 62-63 22.482584725842987 22.925338689687592 25.74853626223343 28.843540322235995 64-65 23.01935980170093 23.336894739091417 24.899354673276637 28.744390785931024 66-67 22.98559767511432 23.60015385272875 24.160861575280997 29.25338689687593 68-69 22.754391213299712 23.75443395016881 24.398905936151117 29.092268900380358 70-71 23.467669558528144 23.619812812513356 24.588230266250694 28.324287362707807 72-73 23.56468225137826 23.4146758408479 24.040343604427537 28.980298303346295 74-75 23.262532586862687 24.057865720757295 24.074533099705114 28.605068592674897 76 23.586478054617718 23.98393093721954 23.32407367836232 29.105517329800417 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 3.0 16 7.0 17 11.0 18 14.0 19 19.5 20 18.5 21 15.0 22 32.0 23 61.0 24 110.0 25 147.0 26 209.0 27 296.5 28 375.5 29 429.0 30 554.5 31 816.5 32 1125.0 33 1297.0 34 1504.0 35 1954.5 36 2377.0 37 2556.0 38 2924.5 39 3782.0 40 4593.5 41 5099.0 42 5282.0 43 5483.0 44 5915.5 45 6220.5 46 6294.0 47 6297.5 48 6032.5 49 5542.0 50 5320.0 51 4939.5 52 4111.0 53 3432.5 54 3202.0 55 3033.0 56 2647.0 57 2224.5 58 2019.0 59 2001.5 60 1968.5 61 2021.5 62 2090.0 63 2148.5 64 2241.5 65 2346.0 66 2537.5 67 2659.0 68 2630.0 69 2619.0 70 2630.5 71 2624.0 72 2543.5 73 2334.0 74 2099.5 75 1994.0 76 1713.5 77 1281.0 78 923.5 79 718.0 80 573.5 81 322.5 82 157.0 83 98.0 84 66.0 85 24.0 86 9.0 87 4.0 88 3.5 89 3.0 90 2.0 91 2.0 92 3.0 93 2.5 94 2.0 95 1.0 96 0.0 97 1.0 98 3.0 99 14.5 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 116995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.03359117911022 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8048048048048 52.69797854609172 2 10.764212488350418 14.215992136416086 3 3.602309205757482 7.136202401812043 4 1.8186289738013877 4.803624086499423 5 1.0989437713575645 3.628360186332749 6 0.7882882882882882 3.123210393606564 7 0.5488246867557213 2.5368605495961365 8 0.40385212799005904 2.13342450532074 9 0.2886507196852024 1.715457925552374 >10 0.8814849332090712 8.008889268772169 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005128424291636395 0.0 2 0.0 0.0 0.0 0.006837899055515193 0.0 3 0.0 0.0 0.0 0.006837899055515193 0.0 4 0.0 0.0 0.0 0.02906107098593957 0.0 5 0.0 0.0 0.0 0.02906107098593957 0.0 6 0.0 0.0 0.0 0.02991580836787897 0.0 7 0.0 0.0 0.0 0.02991580836787897 0.0 8 0.0 0.0 0.0 0.02991580836787897 0.0 9 0.0 0.0 0.0 0.033334757895636564 0.0 10 0.0 0.0 0.0 0.035898970041454766 0.0 11 0.0 0.0 0.0 0.035898970041454766 0.0 12 0.0 0.0 0.0 0.035898970041454766 0.0 13 0.0 0.0 0.0 0.03760844480533356 0.0 14 0.0 0.0 0.0 0.03931791956921236 0.0 15 0.0 0.0 0.0 0.03931791956921236 0.0 16 0.0 0.0 0.0 0.040172656951151756 0.0 17 0.0 0.0 0.0 0.04102739433309116 0.0 18 0.0 0.0 0.0 0.04273686909696996 0.0 19 0.0 0.0 0.0 0.046155818624727556 0.0 20 0.0 0.0 0.0 0.04786529338860635 0.0 21 0.0 0.0 0.0 0.05299371768024275 0.0 22 0.0 0.0 0.0 0.05299371768024275 0.0 23 0.0 0.0 0.0 0.05470319244412154 0.0 24 0.0 0.0 0.0 0.05812214197187914 0.0 25 0.0 0.0 0.0 0.060686354117697336 0.0 26 0.0 0.0 0.0 0.06837899055515193 0.0 27 0.0 0.0 0.0 0.1085516475063037 0.0 28 0.0 0.0 0.0 0.1803495875892132 0.0 29 0.0 0.0 0.0 0.24701910338048635 0.0 30 0.0 0.0 0.0 0.30941493226206246 0.0 31 0.0 0.0 0.0 0.3829223471088508 0.0 32 0.0 0.0 0.0 0.4735245095944271 0.0 33 0.0 0.0 0.0 0.5940424804478824 0.0 34 0.0 0.0 0.0 0.7812299670926108 0.0 35 0.0 0.0 0.0 1.0521817171674004 0.0 36 0.0 0.0 0.0 1.4333945895123723 0.0 37 0.0 0.0 0.0 1.9265780588914057 0.0 38 0.0 0.0 0.0 2.497542630026924 0.0 39 0.0 0.0 0.0 3.071071413308261 0.0 40 0.0 0.0 0.0 3.6608402068464465 0.0 41 0.0 0.0 0.0 4.335228001196632 0.0 42 0.0 0.0 0.0 5.030984230095303 0.0 43 0.0 0.0 0.0 5.739561519723065 0.0 44 0.0 0.0 0.0 6.446429334586949 0.0 45 0.0 0.0 0.0 7.155861361596649 0.0 46 0.0 0.0 0.0 7.926834480105987 0.0 47 0.0 0.0 0.0 8.645668618317021 0.0 48 0.0 0.0 0.0 9.276464806188299 0.0 49 0.0 0.0 0.0 9.85768622590709 0.0 50 0.0 0.0 0.0 10.45514765588273 0.0 51 0.0 0.0 0.0 11.10987649044831 0.0 52 0.0 0.0 0.0 11.774007436215223 0.0 53 0.0 0.0 0.0 12.456942604384803 0.0 54 0.0 0.0 0.0 13.078336681054745 0.0 55 0.0 0.0 0.0 13.64246335313475 0.0 56 0.0 0.0 0.0 14.232232146672935 0.0 57 0.0 0.0 0.0 14.812598829009787 0.0 58 0.0 0.0 0.0 15.372451814180094 0.0 59 0.0 0.0 0.0 15.892132142399248 0.0 60 0.0 0.0 0.0 16.426343006111374 0.0 61 0.0 0.0 0.0 16.993033890337195 0.0 62 0.0 0.0 0.0 17.504166844736954 0.0 63 0.0 0.0 0.0 18.064874567289202 0.0 64 0.0 0.0 0.0 18.6332749262789 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTACG 15 0.0022198975 70.0 4 CCGTACC 20 0.006936511 52.5 55 ATTCAGA 20 0.006936511 52.5 19 TGCTATT 20 0.006936511 52.5 10 GTCTATT 45 0.004292807 31.111109 1 TAGATCT 45 0.004292807 31.111109 39 TATAACC 45 0.004292807 31.111109 3 TTTTGAG 60 4.957292E-4 29.166666 50 GCTCTAA 50 0.0071843304 27.999998 1 AATGATG 50 0.0071843304 27.999998 24 AGTGTTG 50 0.0071843304 27.999998 26 CGGACGC 50 0.0071843304 27.999998 32 TGTAGAT 75 0.0018232497 23.333334 37 TACCCCT 95 2.7607678E-4 22.105265 5 CCCAACA 100 3.9045687E-4 21.0 34 TAAGAGA 85 0.0037707398 20.588236 4 ATAATAT 90 0.0052491324 19.444443 3 ACGAAGT 110 7.4223534E-4 19.09091 70 GATGCAC 355 1.8189894E-12 16.760565 70 AGAGGGA 140 0.003727135 15.000001 6 >>END_MODULE