FastQCFastQC Report
Wed 25 May 2016
SRR1780184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116995
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT36593.1274840805162616No Hit
CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG11841.0120090602162484TruSeq Adapter, Index 2 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC10930.9342279584597633No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG9380.8017436642591564No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2880.24616436599854694No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT1630.13932219325612205TruSeq Adapter, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC150.002219897570.038
ACAAGCG207.898922E-570.053
TCTTTAT401.05610525E-861.251
CTTTATA401.05610525E-861.252
TCGCTAC252.3821366E-455.99999639
CGTAACT252.3821366E-455.99999634
ACGTAAC252.3821366E-455.99999633
TAACTCG252.3821366E-455.99999636
GTGCATC200.00693651152.51
TGCATAA200.00693651152.530
CTGCATA200.00693651152.529
GCCCAAG305.8576494E-446.6666641
TTAATAC305.8576494E-446.6666643
TATAAGA404.5694123E-543.752
CGCCTAT350.001251149940.00000458
TGACAAG350.001251149940.00000451
AACTCGT350.001251149940.00000437
ACATCGT350.001251149940.00000423
CACGTAA350.001251149940.00000432
CCTATAG350.001251149940.00000460