FastQCFastQC Report
Wed 25 May 2016
SRR1780183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721276
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTC655.9675403E-727.05507942
GATACTA701.1405737E-625.1190871
CTCCAGA551.6029987E-423.977311
CGTCAAA651.803949E-523.70606612
TCAGATG651.8244076E-523.6715534
TAGTGTC500.002557309622.03266119
TGGATTG500.002571791922.01124637
AAACTCG500.002585299821.99139827
ATTTCGA500.002585299821.99139827
TATCAGG654.961047E-420.2884962
TTGTACT1001.1316188E-619.7826544
ATTGCGA708.038384E-418.8851415
GCGTCAA1051.7937782E-618.85369311
TTAAGGC951.601036E-518.51907230
TTGCGAT600.00734613418.36055216
AGTGTCC600.00734613418.36055220
GTACAAT600.0074408718.31981331
CTTAGCG600.0074408718.31981341
GTATAAG2300.018.1567331
GATAAAG851.4136606E-418.14454516
Produced by FastQC (version 0.11.5)