FastQCFastQC Report
Wed 25 May 2016
SRR1780183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721276
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT184152.5531142031621736No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC61860.8576467260798918No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG47860.6635462707756808No Hit
CACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTTCTG38260.5304488157099363RNA PCR Primer, Index 31 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC11450.15874644380237246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG4950.036.8856344
CGTCTTC5700.032.41819442
CCGTCTT5750.032.13629541
GTCTTCT5800.031.47998243
ATGCCGT27350.031.28791644
CTTTATA1400.029.8669322
GTATGAC300.00568973529.389831
TATGCCG30550.028.0826343
CGCAAAG508.3418534E-526.3976712
GCCGTCT7000.026.39766940
CCGTATT609.752535E-625.66440214
TCCGTAT609.752535E-625.66440213
CGATTAG551.5937026E-423.99788319
CTTTTGG651.813314E-523.69021836
TGTATAA651.813314E-523.69021814
CGGTATG651.813314E-523.69021843
CGTATTA752.063929E-623.46459615
CGTATTC500.002579720721.99958624
CTCATGT602.872936E-421.9980641
CAAAGGC602.872936E-421.9980614
Produced by FastQC (version 0.11.5)