FastQCFastQC Report
Wed 25 May 2016
SRR1780182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117107
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGA150.002219903770.0000161
GTATCAT252.3821488E-456.00000457
GTTTCGT200.00693653452.56
AACGCGT200.00693653452.556
GTATCGT200.00693653452.533
CAAGAAT200.00693653452.514
CGTTTCG200.00693653452.55
TCTGGGC200.00693653452.558
CGTGCAA404.569449E-543.7510
CGTATCA350.001251156840.056
CTTATAA350.001251156840.01
GATGGGA350.001251156840.026
CGAGGTA350.001251156840.067
GACGAAT400.002410601135.023
ATGTTTA400.002410601135.068
CCTCTAG652.0880245E-532.3076931
TCCATAC450.00429282931.1111094
AGATCTC703.4644192E-530.040
TCAAGGA500.00718436628.0000022
TGCAAGA500.00718436628.00000212
Produced by FastQC (version 0.11.5)