##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780182_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117107 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.092103802505402 34.0 31.0 34.0 12.0 34.0 2 30.286677995337598 34.0 31.0 34.0 12.0 34.0 3 30.106500892346315 34.0 31.0 34.0 11.0 34.0 4 29.93342840308436 34.0 31.0 34.0 11.0 34.0 5 29.736283911294798 34.0 31.0 34.0 11.0 34.0 6 32.88868299930832 37.0 33.0 38.0 11.0 38.0 7 33.097013842041896 37.0 34.0 38.0 12.0 38.0 8 33.050927783992414 37.0 34.0 38.0 11.0 38.0 9 33.51107107175489 38.0 34.0 38.0 21.0 38.0 10-11 33.22930311595378 38.0 34.0 38.0 16.0 38.0 12-13 33.31212480893542 38.0 34.0 38.0 21.0 38.0 14-15 33.25467734635846 38.0 34.0 38.0 11.0 38.0 16-17 33.32821692981632 38.0 34.0 38.0 11.0 38.0 18-19 33.34779304396834 38.0 34.0 38.0 16.0 38.0 20-21 33.34984672137447 38.0 34.0 38.0 16.0 38.0 22-23 33.37237739844757 38.0 34.0 38.0 16.0 38.0 24-25 33.41118805878385 38.0 34.0 38.0 16.0 38.0 26-27 33.36693792856106 38.0 34.0 38.0 16.0 38.0 28-29 33.466974647117596 38.0 34.0 38.0 21.0 38.0 30-31 33.4973357698515 38.0 34.0 38.0 21.0 38.0 32-33 33.554074478895366 38.0 34.0 38.0 21.0 38.0 34-35 33.52781644137413 38.0 34.0 38.0 21.0 38.0 36-37 33.459605318213264 38.0 34.0 38.0 21.0 38.0 38-39 33.44720640098372 38.0 34.0 38.0 16.0 38.0 40-41 33.51240318682913 38.0 34.0 38.0 21.0 38.0 42-43 33.59572869256321 38.0 34.0 38.0 21.0 38.0 44-45 33.65175864807398 38.0 34.0 38.0 21.0 38.0 46-47 33.5634163628135 38.0 34.0 38.0 21.0 38.0 48-49 33.515280896957485 38.0 34.0 38.0 16.0 38.0 50-51 33.42589682939534 38.0 34.0 38.0 14.5 38.0 52-53 33.49708813307488 38.0 34.0 38.0 21.0 38.0 54-55 33.46877214854791 38.0 34.0 38.0 21.0 38.0 56-57 33.43148573526774 38.0 34.0 38.0 20.0 38.0 58-59 33.49424884934291 38.0 34.0 38.0 20.5 38.0 60-61 33.48971453457095 38.0 34.0 38.0 20.0 38.0 62-63 33.37823955869419 38.0 34.0 38.0 19.0 38.0 64-65 33.31818337076349 38.0 34.0 38.0 15.0 38.0 66-67 33.244477272921344 38.0 33.5 38.0 19.0 38.0 68-69 33.147156019708476 38.0 33.5 38.0 14.5 38.0 70-71 33.129680548558156 38.0 33.5 38.0 11.0 38.0 72-73 33.19351106253256 38.0 34.0 38.0 15.0 38.0 74-75 33.237116483216205 38.0 34.0 38.0 15.0 38.0 76 31.6247192738265 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05079620010059571 1101 2 0.5819343686316785 1101 3 -0.16284949043772912 1101 4 0.6473651659062263 1101 5 -0.10163098641026735 1101 6 -0.417820398118387 1101 7 -0.5187426375022994 1101 8 -0.5080785877870966 1101 9 -0.1026484301515751 1101 10-11 -0.3435771724125942 1101 12-13 -0.1352258111237319 1101 14-15 -0.014584043311948847 1101 16-17 -0.35352282030431326 1101 18-19 0.0016998443580575895 1101 20-21 0.06497613758241982 1101 22-23 -0.2736020563030692 1101 24-25 -0.11321705153060435 1101 26-27 -0.29076384428953617 1101 28-29 -0.4030791386667829 1101 30-31 -0.4701513287123831 1101 32-33 0.09269862935143891 1101 34-35 -0.24260268925314676 1101 36-37 0.13062680076483701 1101 38-39 -0.0264825960819266 1101 40-41 0.0022696502565864307 1101 42-43 -0.2592893661319877 1101 44-45 0.19120762892788434 1101 46-47 0.17082202467946672 1101 48-49 0.13694093672442875 1101 50-51 0.12682633465786353 1101 52-53 0.3445776782796912 1101 54-55 0.4807322502728937 1101 56-57 -0.01785316181451435 1101 58-59 -0.18242639152106932 1101 60-61 0.06213336799154234 1101 62-63 0.2720493235829764 1101 64-65 0.4855508778285369 1101 66-67 0.06882023883183308 1101 68-69 -0.10248279935053262 1101 70-71 0.01608003900567212 1101 72-73 -0.13827234102699748 1101 74-75 -0.03494020067258674 1101 76 -1.2766805489252029 1102 1 -0.2896497032853098 1102 2 -0.17609047843901493 1102 3 0.06001738223679709 1102 4 -0.25699883737308227 1102 5 -0.5411424534805427 1102 6 0.06054877637868117 1102 7 -1.0323748924475424 1102 8 -0.5002902293334159 1102 9 -0.3443713285560648 1102 10-11 -0.3322362294010972 1102 12-13 -0.04007855754487366 1102 14-15 -0.3892398209916266 1102 16-17 -0.4651349369299922 1102 18-19 -0.2339742007534582 1102 20-21 -0.36404049019500917 1102 22-23 -0.644642185793721 1102 24-25 -0.26462231944593384 1102 26-27 -0.6876915943893849 1102 28-29 -0.35698487955934866 1102 30-31 -0.337812298631043 1102 32-33 -0.38844534491860117 1102 34-35 -0.11123589015285518 1102 36-37 0.10677298226196541 1102 38-39 0.04699178957319816 1102 40-41 -0.09747073779495707 1102 42-43 -0.5323759104552082 1102 44-45 -0.4827166106298293 1102 46-47 -1.0474359476145736 1102 48-49 -0.8586813962094979 1102 50-51 -0.43145497117972553 1102 52-53 -0.24972347158050923 1102 54-55 -0.546195920251165 1102 56-57 -0.30396234598133276 1102 58-59 -0.7188035961205159 1102 60-61 -0.1463182096503033 1102 62-63 -0.6339648447128354 1102 64-65 -0.3255472702028257 1102 66-67 -0.25224196292974455 1102 68-69 -0.31328349725472293 1102 70-71 -0.05748368679366678 1102 72-73 0.013316862479342717 1102 74-75 -0.756850608273858 1102 76 -0.47770638399278553 1103 1 0.1940415580114987 1103 2 0.26101378099769335 1103 3 0.7964659101288767 1103 4 0.7069171388026305 1103 5 -0.03652978602474377 1103 6 -0.7915170598199524 1103 7 -0.28733985120597083 1103 8 -0.23426802088970078 1103 9 0.0948748620006512 1103 10-11 -0.32113200754212556 1103 12-13 0.17317980819925793 1103 14-15 -0.12375779254527686 1103 16-17 0.012215080919425247 1103 18-19 0.09098399401195678 1103 20-21 0.003671777057867587 1103 22-23 -0.01676044293818535 1103 24-25 0.2899781352447093 1103 26-27 -0.11695619803818857 1103 28-29 -0.024680236829695446 1103 30-31 -0.09941912274843645 1103 32-33 -0.03611646454733375 1103 34-35 -0.29678067342324965 1103 36-37 -0.515697566185132 1103 38-39 -0.9792703527336997 1103 40-41 -0.0726741273070175 1103 42-43 0.01317770358383541 1103 44-45 0.08111387618977517 1103 46-47 -0.12609085244895368 1103 48-49 -0.05386486951991287 1103 50-51 -0.3712460389511776 1103 52-53 -0.28252883768163883 1103 54-55 -0.282447165013771 1103 56-57 -0.25295056536018024 1103 58-59 -0.5719967644875723 1103 60-61 0.05627747266749594 1103 62-63 -0.285093116324731 1103 64-65 -0.09869515739445944 1103 66-67 -0.046837344625629385 1103 68-69 0.5819121888208443 1103 70-71 0.09436795629297734 1103 72-73 0.13004741130221475 1103 74-75 0.14835954523120876 1103 76 0.011762454667191946 1104 1 -0.23885590159313352 1104 2 -0.10507612346230744 1104 3 -0.5035913258152966 1104 4 0.3092241876292938 1104 5 -0.3147140857649795 1104 6 -0.30719530575839826 1104 7 -0.43895346455515494 1104 8 -0.41004400820973785 1104 9 -0.7601332831144845 1104 10-11 -0.05688686159046341 1104 12-13 -0.077548849397715 1104 14-15 -0.07945276899048537 1104 16-17 -0.3257015307126707 1104 18-19 -0.6821679454059932 1104 20-21 -0.6850263253504423 1104 22-23 -0.5463866481950888 1104 24-25 -0.18304612399008846 1104 26-27 -0.5828479780760532 1104 28-29 -0.140486162951305 1104 30-31 -0.26818590828975175 1104 32-33 0.023761138587431674 1104 34-35 -0.17110374831428032 1104 36-37 -0.24527093826279867 1104 38-39 -0.29180942727250425 1104 40-41 -0.5827212368918353 1104 42-43 -0.38153457572757077 1104 44-45 -0.36553341831116626 1104 46-47 -0.1199666064224516 1104 48-49 -0.1534289957912094 1104 50-51 -0.07442483523524146 1104 52-53 -0.21038513143410853 1104 54-55 -0.21901653057534531 1104 56-57 -0.05104857090766046 1104 58-59 -0.26899848156589456 1104 60-61 -0.22688195145084933 1104 62-63 -0.5334134615015031 1104 64-65 -0.0867460533571176 1104 66-67 0.13991033291586774 1104 68-69 -0.27741506869687527 1104 70-71 0.2825201158491737 1104 72-73 0.38185474630860483 1104 74-75 0.03839608818017837 1104 76 0.063371665251438 1105 1 0.9313466570422619 1105 2 1.0192575652370515 1105 3 0.7595927537866913 1105 4 1.2577494186172125 1105 5 0.6185481743629531 1105 6 1.3042741327632754 1105 7 1.553157409645273 1105 8 0.504828060020543 1105 9 0.9101403487263156 1105 10-11 1.2358370190065457 1105 12-13 -0.00686654662585795 1105 14-15 0.8159941037884764 1105 16-17 0.8901122574891716 1105 18-19 1.1028755869323135 1105 20-21 0.8205307108968896 1105 22-23 0.9450890092320563 1105 24-25 0.7539741318734485 1105 26-27 1.1693375229900411 1105 28-29 0.9255541710927631 1105 30-31 0.9350967853847365 1105 32-33 0.7229437967253105 1105 34-35 0.81021732489112 1105 36-37 0.6054175834145283 1105 38-39 1.21075809227262 1105 40-41 0.8699617766120795 1105 42-43 1.2236049054380302 1105 44-45 1.1455628858821498 1105 46-47 1.3401702094979484 1105 48-49 0.6655830866537045 1105 50-51 0.9158114831727104 1105 52-53 0.9745429069454232 1105 54-55 0.7787491055298474 1105 56-57 0.5594835897177859 1105 58-59 0.24534942177454155 1105 60-61 1.107188766388525 1105 62-63 0.8376286493727036 1105 64-65 0.7175316651929862 1105 66-67 0.7639375184169381 1105 68-69 0.726165244025232 1105 70-71 1.059838879175409 1105 72-73 1.0694569140413677 1105 74-75 0.7045252637309929 1105 76 1.2579434491888932 1106 1 -0.20049679890298933 1106 2 0.6740372914988164 1106 3 0.7226666060821003 1106 4 0.8969353325037233 1106 5 0.03086639506440747 1106 6 0.6465360375251805 1106 7 0.17077930870429725 1106 8 0.22349786327118437 1106 9 0.45779503934507915 1106 10-11 0.030372947150262064 1106 12-13 0.054938414879700304 1106 14-15 0.01973840090851553 1106 16-17 -0.11316689848458594 1106 18-19 0.20024335140337257 1106 20-21 0.054892344771992896 1106 22-23 -0.4375118604571355 1106 24-25 -0.1316653035414319 1106 26-27 -0.38295075759165087 1106 28-29 0.13289841118401569 1106 30-31 -0.4307696959383307 1106 32-33 0.1826672070475297 1106 34-35 0.08571098043717029 1106 36-37 -0.08608334330346423 1106 38-39 -0.26744621340174746 1106 40-41 0.5172742944335695 1106 42-43 0.3061095390279931 1106 44-45 0.07240562896740244 1106 46-47 0.011452644627695463 1106 48-49 0.1289420829468355 1106 50-51 0.08210389834237475 1106 52-53 -0.3919295053728007 1106 54-55 -0.21078076330391582 1106 56-57 -0.5110449959679784 1106 58-59 -0.16217481546526358 1106 60-61 0.12117864377660226 1106 62-63 -0.020449836512668185 1106 64-65 0.08170510402959508 1106 66-67 -0.21240444052232732 1106 68-69 0.3387436586246011 1106 70-71 0.07689405453896114 1106 72-73 0.20809927218222413 1106 74-75 0.0673799115781506 1106 76 -0.1323652191886353 1107 1 -0.6284359516533904 1107 2 -0.7295778384896572 1107 3 -1.0116287783044058 1107 4 -0.7813292563310306 1107 5 -0.3783462355749343 1107 6 -1.2882465711704718 1107 7 -0.40646991954727696 1107 8 -1.2211036169980929 1107 9 -0.7003668976504969 1107 10-11 -0.7497996269561895 1107 12-13 -0.6512764845140566 1107 14-15 -0.8055768071846146 1107 16-17 -0.46910099302842667 1107 18-19 -0.04729263252938409 1107 20-21 -0.2635143201590111 1107 22-23 -0.33603159118201376 1107 24-25 -0.3985879799277896 1107 26-27 -0.7413767627242365 1107 28-29 -0.6683252284931598 1107 30-31 -0.38134990198586394 1107 32-33 -0.14092783888545313 1107 34-35 -0.47051507676519577 1107 36-37 -0.5257288969167391 1107 38-39 -0.787818512282243 1107 40-41 -0.4035889480256074 1107 42-43 -0.5111906432162172 1107 44-45 -0.745870861529859 1107 46-47 -0.462754221143868 1107 48-49 -0.5616425862241385 1107 50-51 -0.4916678128935317 1107 52-53 -0.18947106820985482 1107 54-55 -0.21510441413889225 1107 56-57 -0.08770843926772187 1107 58-59 -0.539717866010136 1107 60-61 -0.7850213785390139 1107 62-63 0.10412709657767039 1107 64-65 -0.1009854155523584 1107 66-67 -0.2723590654340029 1107 68-69 -0.2607653977967175 1107 70-71 -0.17967975436497596 1107 72-73 -0.09934484579299863 1107 74-75 -0.11580341121725013 1107 76 -0.3027449566082083 1108 1 -0.28946689496464373 1108 2 -0.0993494697219468 1108 3 -0.2761574979756425 1108 4 0.29466856694119414 1108 5 0.4033992749240234 1108 6 0.23414933057654252 1108 7 0.8317504178455266 1108 8 -0.26982666591817406 1108 9 -0.13429570364025523 1108 10-11 -0.014351802745714792 1108 12-13 -0.11379360748041023 1108 14-15 -0.41474158172439957 1108 16-17 -0.2473140121123194 1108 18-19 -0.22778277157725313 1108 20-21 -0.0036627504755699647 1108 22-23 -0.026879952068476598 1108 24-25 -0.4771706674360061 1108 26-27 -0.28507551110576657 1108 28-29 0.16084855319606106 1108 30-31 -0.2582521931794943 1108 32-33 0.03825813998528105 1108 34-35 -0.4789452056800201 1108 36-37 -0.2991240293030657 1108 38-39 -0.15570896282736157 1108 40-41 -0.046183382357860125 1108 42-43 -0.1483244932096497 1108 44-45 0.1377492619431422 1108 46-47 -0.4037245538454499 1108 48-49 -0.06053545744067179 1108 50-51 -0.4154448108856954 1108 52-53 -0.7815128763951051 1108 54-55 -0.2295815303498827 1108 56-57 -0.18716707205749827 1108 58-59 -0.2653235029844794 1108 60-61 0.025410964633380217 1108 62-63 -0.20616450988495671 1108 64-65 -0.10623020360286972 1108 66-67 0.04114133801123643 1108 68-69 -0.15902213908511698 1108 70-71 -0.23323945487724984 1108 72-73 -0.1699196145156776 1108 74-75 -0.45488974849746455 1108 76 -0.3278805329719461 1109 1 -0.3714311207355152 1109 2 -0.2876868792073921 1109 3 0.4183229125168495 1109 4 0.4359240217918128 1109 5 -0.46557880976403254 1109 6 0.14971064069978723 1109 7 0.29125264774050663 1109 8 -0.2320767018842247 1109 9 0.2930768566628217 1109 10-11 0.2919878126573394 1109 12-13 -0.1965490863083943 1109 14-15 0.3732361672805382 1109 16-17 0.39884241621933114 1109 18-19 0.527379555186279 1109 20-21 0.0726140442302281 1109 22-23 0.38308504097808793 1109 24-25 -0.12995443955512798 1109 26-27 0.23243276108528477 1109 28-29 0.2458914726800714 1109 30-31 0.6347991663371104 1109 32-33 0.3094517332332529 1109 34-35 0.548014943938739 1109 36-37 0.2924215516684896 1109 38-39 0.48675015576468894 1109 40-41 -0.23202235037205554 1109 42-43 0.5183469689300964 1109 44-45 0.21996764778690903 1109 46-47 0.6764797333074739 1109 48-49 0.22351959459020776 1109 50-51 0.33704164190287855 1109 52-53 -0.06027852162601022 1109 54-55 0.3556340261647648 1109 56-57 0.21632882781302953 1109 58-59 0.45507164399676725 1109 60-61 0.22087924257900227 1109 62-63 0.11768817318222347 1109 64-65 0.30929753820885963 1109 66-67 0.1860605342899504 1109 68-69 0.20334778370776974 1109 70-71 0.19495792679445145 1109 72-73 0.1399926283270787 1109 74-75 0.4355768510325788 1109 76 0.4140942428396883 1110 1 0.4383678865867111 1110 2 0.3552538058385579 1110 3 0.3101603492459297 1110 4 0.304723471396219 1110 5 0.4223960292943971 1110 6 0.6821397526954627 1110 7 0.5233033627633574 1110 8 0.7083331462655664 1110 9 0.7459246624518059 1110 10-11 0.15282618309323226 1110 12-13 0.8311476064037748 1110 14-15 0.48419493675324077 1110 16-17 0.6629635032506371 1110 18-19 0.41134436918206063 1110 20-21 0.6558220642803505 1110 22-23 0.818536414509957 1110 24-25 0.6960075241777375 1110 26-27 0.832594931216498 1110 28-29 0.7936481191983233 1110 30-31 0.34749266478912944 1110 32-33 0.3732828797269363 1110 34-35 0.2911669415799025 1110 36-37 0.5907163081675861 1110 38-39 0.4826418457740189 1110 40-41 0.6147465326197405 1110 42-43 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0.6940967363210362 2116 12-13 0.10280028830599974 2116 14-15 0.216491537744389 2116 16-17 0.20186558072659722 2116 18-19 0.5253211110783695 2116 20-21 -0.12528264094560626 2116 22-23 0.09473460068418404 2116 24-25 0.0780213302581032 2116 26-27 0.7309856804862633 2116 28-29 0.03316501578399311 2116 30-31 0.5719016398353531 2116 32-33 0.21768550966491063 2116 34-35 0.4185012303260862 2116 36-37 0.16557764567264854 2116 38-39 0.5744107087962647 2116 40-41 -0.37698841689289253 2116 42-43 0.2898540641120988 2116 44-45 -0.05943669345488445 2116 46-47 0.4966042495450651 2116 48-49 0.008128938916875939 2116 50-51 0.1785470544330181 2116 52-53 0.27349230119075685 2116 54-55 0.13271069900115862 2116 56-57 0.16902948404725038 2116 58-59 0.2868793729776371 2116 60-61 0.5897838362185723 2116 62-63 0.2692446261521013 2116 64-65 -0.2805280268817256 2116 66-67 0.07276197576170773 2116 68-69 0.24712477624801465 2116 70-71 0.4563242401042302 2116 72-73 0.5875611963903467 2116 74-75 0.4673397092971996 2116 76 0.5702331177064792 2117 1 0.5935699970825041 2117 2 0.5970996175509349 2117 3 0.9509073077773031 2117 4 0.5876454615414239 2117 5 0.9691517960933353 2117 6 0.7979444882294047 2117 7 1.1215678724219273 2117 8 1.3461258467408612 2117 9 1.1877225083507241 2117 10-11 1.300097582386833 2117 12-13 1.2298034734948686 2117 14-15 1.382245780515504 2117 16-17 0.6666045445448461 2117 18-19 0.9058392019557928 2117 20-21 0.45502517769769923 2117 22-23 0.7936595994399696 2117 24-25 0.9497739306662041 2117 26-27 1.2553536195695258 2117 28-29 0.7374463575448118 2117 30-31 1.3904329691540127 2117 32-33 0.62343004756368 2117 34-35 0.9260162853203653 2117 36-37 0.8519174157418163 2117 38-39 1.4476377032719512 2117 40-41 1.1684191877455419 2117 42-43 0.9893451804212603 2117 44-45 1.1198670441651828 2117 46-47 1.4983274864932525 2117 48-49 1.2159687338703407 2117 50-51 1.3034288540647339 2117 52-53 1.1349428063417974 2117 54-55 1.6305893134440055 2117 56-57 0.9293066950162441 2117 58-59 1.453305491526386 2117 60-61 1.182851073564919 2117 62-63 1.0504681865960421 2117 64-65 1.0062011822956052 2117 66-67 1.6986080415557652 2117 68-69 1.1738094425497039 2117 70-71 1.2485964244470367 2117 72-73 -0.24050703632751436 2117 74-75 1.264489214099875 2117 76 0.34794177246186564 2118 1 -0.2544999038042981 2118 2 -0.051178942699454666 2118 3 0.2373705315644692 2118 4 -0.00481671894892699 2118 5 0.12807198388676255 2118 6 0.49838788937703526 2118 7 0.16215211863998036 2118 8 0.601785731978211 2118 9 0.3110662746522408 2118 10-11 -0.5387000180304895 2118 12-13 -0.19866548842479403 2118 14-15 -0.32411833007396496 2118 16-17 0.04328686066377685 2118 18-19 -0.2834670056602775 2118 20-21 -0.1030473314311493 2118 22-23 0.2542490621421152 2118 24-25 -0.17386978347046522 2118 26-27 -0.1466677680152415 2118 28-29 -0.06574873896014566 2118 30-31 0.014428795966743735 2118 32-33 -0.021236097454575997 2118 34-35 -0.06759352166972121 2118 36-37 0.03422049346743705 2118 38-39 5.067695213014645E-4 2118 40-41 0.26506759671789126 2118 42-43 0.05459035517348099 2118 44-45 0.22578775360702252 2118 46-47 -0.44362608679834636 2118 48-49 -0.05981373874312368 2118 50-51 0.0060892609504961115 2118 52-53 -0.01345312480061267 2118 54-55 -0.04939242886168671 2118 56-57 -0.3566870452028468 2118 58-59 -0.22217703325190996 2118 60-61 -0.4791207574210006 2118 62-63 -0.146597541103489 2118 64-65 0.056122935034260024 2118 66-67 -0.4073257090962983 2118 68-69 -0.16702258666260406 2118 70-71 -0.545782813946289 2118 72-73 -0.03752059918614492 2118 74-75 0.05594722140295261 2118 76 0.176528105273551 2119 1 0.9492037998994043 2119 2 0.3377099461894346 2119 3 -0.3351042159102775 2119 4 -0.49387395800616574 2119 5 0.6629204687352477 2119 6 0.48365724964639156 2119 7 0.5125729940608537 2119 8 0.5854557656482484 2119 9 0.4896858032717688 2119 10-11 0.26424610991563924 2119 12-13 0.569432154672846 2119 14-15 1.1941055756499495 2119 16-17 1.041897971774894 2119 18-19 0.9011710414777738 2119 20-21 0.24653179314797313 2119 22-23 0.42655545944850815 2119 24-25 0.6841689370682573 2119 26-27 0.8314046225571445 2119 28-29 0.3047478940364883 2119 30-31 0.4116931057310538 2119 32-33 0.43966457594609665 2119 34-35 0.7156556365794273 2119 36-37 0.6731935574405057 2119 38-39 0.502695321709858 2119 40-41 0.6324076640579577 2119 42-43 0.8714674595505798 2119 44-45 0.8969156997349614 2119 46-47 0.504942936770675 2119 48-49 0.5687216147922314 2119 50-51 0.6184629983242331 2119 52-53 0.5769509156034331 2119 54-55 0.2897068977376378 2119 56-57 0.6805604295446273 2119 58-59 0.7280334044585217 2119 60-61 0.1951216668214215 2119 62-63 0.43408427707832686 2119 64-65 0.14677950069082613 2119 66-67 0.29221132794074123 2119 68-69 0.41420636956635093 2119 70-71 0.20783671467324183 2119 72-73 0.632092195426651 2119 74-75 -0.08538274492700992 2119 76 0.5834304621759046 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 24.0 13 188.0 14 747.0 15 1442.0 16 2002.0 17 2397.0 18 2472.0 19 2325.0 20 1744.0 21 1399.0 22 1140.0 23 953.0 24 908.0 25 968.0 26 1163.0 27 1293.0 28 1477.0 29 1850.0 30 2236.0 31 2714.0 32 3537.0 33 4441.0 34 6000.0 35 8784.0 36 16565.0 37 48338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.41364734815169 20.227655050509362 11.866925119762268 30.491772481576678 2 19.39679096894293 19.137199313448384 34.65377816868334 26.812231548925343 3 18.59666800447454 23.62369456992323 28.08798790849394 29.69164951710829 4 14.914565312065035 18.458332977533367 31.443893191696482 35.183208518705115 5 15.349210551034522 31.781191559855515 31.8332806749383 21.036317214171653 6 28.631080977225952 34.34978267738052 17.07242094836346 19.946715397030065 7 27.31177470176847 26.18887000777067 22.524699633668355 23.974655656792507 8 23.977217416550676 30.60448991093615 21.34202054531326 24.07627212719991 9 23.534032978387287 16.66168546713689 20.95177914215205 38.85250241232377 10-11 26.240105202934068 22.953367433202114 25.26535561495043 25.54117174891339 12-13 24.94129300554194 20.564526458708702 24.618511276012537 29.87566925973682 14-15 24.243640431400344 21.89066409352131 22.543058911935237 31.322636563143107 16-17 25.536902149316436 23.4904830624984 23.37221515366289 27.600399634522276 18-19 24.46694049032082 22.690787057989702 24.451996891731493 28.39027555995799 20-21 24.051935409497297 23.315002519063764 24.453704731570273 28.179357339868666 22-23 25.72914398432196 23.108025600628487 23.53925700110583 27.62357341394372 24-25 24.60698335710077 23.960565978122574 23.238149726318667 28.19430093845799 26-27 23.965262537679216 24.28206682777289 24.304695705636725 27.44797492891117 28-29 24.549770722501645 23.674502805126934 25.46773463584585 26.307991836525566 30-31 24.616803436173758 22.95294047324242 25.108661309742374 27.32159478084145 32-33 23.895668064248934 24.31451578470971 24.684263109805563 27.10555304123579 34-35 24.373009299187924 24.005396773890546 25.057426114578973 26.56416781234256 36-37 24.816193737351313 22.715977695611706 25.14196418659858 27.325864380438404 38-39 23.702682162466804 23.08828678046573 26.71701947791336 26.492011579154106 40-41 23.404664110599708 22.56013731032304 26.884387782113794 27.15081079696346 42-43 22.353915649790363 22.777459929807783 26.71701947791336 28.151604942488497 44-45 21.982887444815425 22.616069065043078 27.846328571306582 27.55471491883491 46-47 22.20789534357468 23.159162133775094 27.740869461261923 26.892073061388306 48-49 22.10755975304636 23.413630269753302 27.569658517424234 26.909151459776105 50-51 21.85660061567889 24.0345326689808 26.45156332056718 27.657303394773137 52-53 21.27327999180237 23.950745899049586 26.8754216229602 27.900552486187845 54-55 21.177214000870997 23.73940071900057 26.71189595839702 28.371489321731406 56-57 21.443210055760968 23.924701341508193 26.571426131657372 28.06066247107346 58-59 21.816800020494078 23.865353907110592 25.937817551470022 28.38002852092531 60-61 21.841136738196692 23.083163260949387 26.25291400172492 28.822785999129003 62-63 21.43467085656707 23.36538379430777 26.138915692486357 29.061029656638798 64-65 22.061021117439605 23.63351464899622 25.20558122059313 29.099883012971045 66-67 21.883405774206494 24.38496417805938 24.335436822734763 29.39619322499936 68-69 21.766845705209764 24.862732372958064 24.361054420316464 29.009367501515708 70-71 22.49438547653001 24.730801745412315 24.73293654521079 28.041876232846885 72-73 22.128480791071414 24.165079798816468 24.481030168990753 29.22540924112137 74-75 21.933787049450505 24.95410180433279 24.33757162253324 28.77453952368347 76 22.313781413579033 25.121470108533224 23.750928637912335 28.81381983997541 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.0 14 0.5 15 1.0 16 1.5 17 2.0 18 7.0 19 11.5 20 15.5 21 20.0 22 28.0 23 48.0 24 77.5 25 95.0 26 168.5 27 276.0 28 404.0 29 498.0 30 608.5 31 806.5 32 1087.0 33 1280.0 34 1541.0 35 2070.5 36 2492.5 37 2646.0 38 3039.5 39 3939.0 40 4948.0 41 5567.5 42 5684.0 43 5843.5 44 6257.5 45 6565.5 46 6619.0 47 6649.5 48 6288.5 49 5691.0 50 5485.0 51 5050.0 52 4196.5 53 3465.5 54 3153.0 55 3080.0 56 2706.0 57 2279.5 58 2154.0 59 2094.0 60 2090.5 61 2206.5 62 2266.0 63 2263.0 64 2306.5 65 2371.5 66 2432.0 67 2474.0 68 2510.0 69 2498.5 70 2352.0 71 2253.0 72 2220.0 73 1976.5 74 1589.0 75 1412.0 76 1201.0 77 803.0 78 486.0 79 356.0 80 251.5 81 103.5 82 39.0 83 18.0 84 10.0 85 5.5 86 10.0 87 11.0 88 9.0 89 8.5 90 7.0 91 3.0 92 2.0 93 2.5 94 4.5 95 5.5 96 5.0 97 4.5 98 5.5 99 13.5 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.269599596949798E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.269599596949798E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 117107.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.394340218774275 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84020020925827 46.407131939166746 2 11.585555524135396 13.994039638962658 3 4.330797726437237 7.846670139274339 4 2.1222746938890933 5.1269351960173175 5 1.247066142578401 3.765786844509722 6 0.9049006023244633 3.279052490457445 7 0.6772615445522155 2.8631934897145346 8 0.49062579532279504 2.370481696226528 9 0.3859966631790289 2.0980812419411308 >10 1.413907191131974 12.202515648082523 >50 0.0014139071911319742 0.04611167564705782 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005977439435729717 0.0 2 0.0 0.0 0.0 0.005977439435729717 0.0 3 0.0 0.0 0.0 0.005977439435729717 0.0 4 0.0 0.0 0.0 0.017078398387799194 0.0 5 0.0 0.0 0.0 0.02390975774291887 0.0 6 0.0 0.0 0.0 0.02390975774291887 0.0 7 0.0 0.0 0.0 0.024763677662308827 0.0 8 0.0 0.0 0.0 0.02732543742047871 0.0 9 0.0 0.0 0.0 0.030741117098038544 0.0 10 0.0 0.0 0.0 0.03501071669498834 0.0 11 0.0 0.0 0.0 0.03501071669498834 0.0 12 0.0 0.0 0.0 0.03757247645315822 0.0 13 0.0 0.0 0.0 0.04184207605010802 0.0 14 0.0 0.0 0.0 0.04184207605010802 0.0 15 0.0 0.0 0.0 0.04525775572766786 0.0 16 0.0 0.0 0.0 0.04525775572766786 0.0 17 0.0 0.0 0.0 0.04611167564705782 0.0 18 0.0 0.0 0.0 0.046965595566447776 0.0 19 0.0 0.0 0.0 0.050381275244007616 0.0 20 0.0 0.0 0.0 0.053796954921567455 0.0 21 0.0 0.0 0.0 0.053796954921567455 0.0 22 0.0 0.0 0.0 0.05550479476034737 0.0 23 0.0 0.0 0.0 0.05977439435729717 0.0 24 0.0 0.0 0.0 0.06745967363180681 0.0 25 0.0 8.539199193899596E-4 0.0 0.07087535330936665 0.0 26 0.0 8.539199193899596E-4 0.0 0.084538072019606 0.0 27 0.0 8.539199193899596E-4 0.0 0.13491934726361363 0.0 28 0.0 8.539199193899596E-4 0.0 0.19725550137908068 0.0 29 0.0 8.539199193899596E-4 0.0 0.2792318136405168 0.0 30 0.0 8.539199193899596E-4 0.0 0.3697473250958525 0.0 31 0.0 8.539199193899596E-4 0.0 0.45684715687362837 0.0 32 0.0 8.539199193899596E-4 0.0 0.5533401077646938 0.0 33 0.0 8.539199193899596E-4 0.0 0.7172927322875661 0.0 34 0.0 8.539199193899596E-4 0.0 0.9495589503616351 0.0 35 0.0 8.539199193899596E-4 0.0 1.2424534827123912 0.0 36 0.0 8.539199193899596E-4 0.0 1.6463576045838422 0.0 37 0.0 8.539199193899596E-4 0.0 2.11259788057076 0.0 38 0.0 8.539199193899596E-4 0.0 2.7026565448692224 0.0 39 0.0 8.539199193899596E-4 0.0 3.191952658679669 0.0 40 0.0 8.539199193899596E-4 0.0 3.757247645315822 0.0 41 0.0 8.539199193899596E-4 0.0 4.378047426712323 0.0 42 0.0 8.539199193899596E-4 0.0 5.025318725609912 0.0 43 0.0 8.539199193899596E-4 0.0 5.646972426925803 0.0 44 0.0 8.539199193899596E-4 0.0 6.214829173320126 0.0 45 0.0 8.539199193899596E-4 0.0 6.863808312056495 0.0 46 0.0 8.539199193899596E-4 0.0 7.531573689019444 0.0 47 0.0 8.539199193899596E-4 0.0 8.144688191141435 0.0 48 0.0 8.539199193899596E-4 0.0 8.721938056649048 0.0 49 0.0 8.539199193899596E-4 0.0 9.254784086348382 0.0 50 0.0 8.539199193899596E-4 0.0 9.759450758707848 0.0 51 0.0 8.539199193899596E-4 0.0 10.302543827439862 0.0 52 0.0 8.539199193899596E-4 0.0 10.870400573834186 0.0 53 0.0 8.539199193899596E-4 0.0 11.375921166113041 0.0 54 0.0 8.539199193899596E-4 0.0 11.909621115731767 0.0 55 0.0 8.539199193899596E-4 0.0 12.445882825108662 0.0 56 0.0 8.539199193899596E-4 0.0 12.953965177145687 0.0 57 0.0 8.539199193899596E-4 0.0 13.455216169827594 0.0 58 0.0 8.539199193899596E-4 0.0 13.95134364299316 0.0 59 0.0 8.539199193899596E-4 0.0 14.451740715755676 0.0 60 0.0 8.539199193899596E-4 0.0 14.943598589324294 0.0 61 0.0 8.539199193899596E-4 0.0 15.391052627084632 0.0 62 0.0 8.539199193899596E-4 0.0 15.870955621781789 0.0 63 0.0 8.539199193899596E-4 0.0 16.365375255108574 0.0 64 0.0 8.539199193899596E-4 0.0 16.865772327871092 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACAGA 15 0.0022199037 70.00001 61 GTATCAT 25 2.3821488E-4 56.000004 57 GTTTCGT 20 0.006936534 52.5 6 AACGCGT 20 0.006936534 52.5 56 GTATCGT 20 0.006936534 52.5 33 CAAGAAT 20 0.006936534 52.5 14 CGTTTCG 20 0.006936534 52.5 5 TCTGGGC 20 0.006936534 52.5 58 CGTGCAA 40 4.569449E-5 43.75 10 CGTATCA 35 0.0012511568 40.0 56 CTTATAA 35 0.0012511568 40.0 1 GATGGGA 35 0.0012511568 40.0 26 CGAGGTA 35 0.0012511568 40.0 67 GACGAAT 40 0.0024106011 35.0 23 ATGTTTA 40 0.0024106011 35.0 68 CCTCTAG 65 2.0880245E-5 32.307693 1 TCCATAC 45 0.004292829 31.111109 4 AGATCTC 70 3.4644192E-5 30.0 40 TCAAGGA 50 0.007184366 28.000002 2 TGCAAGA 50 0.007184366 28.000002 12 >>END_MODULE