FastQCFastQC Report
Wed 25 May 2016
SRR1780182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117107
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT67955.802385852254775No Hit
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG19171.6369644854705525RNA PCR Primer, Index 23 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC18501.5797518508714252No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG18471.5771900911132553No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4930.42098252025925015No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2710.23141229815467906RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT2070.17676142331372163No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1380.11784094887581442No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1290.11015566960130478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAAAG150.002219903770.000013
TGTCTTC150.002219903770.0000148
TCTTTAT800.065.6251
CTTTATA900.058.333332
TTTATAC1250.053.1999973
TTGGACT200.00693653452.570
ATAGGAC200.00693653452.521
GGGTATC200.00693653452.59
TCTTAAA200.00693653452.557
GGCATAA200.00693653452.51
CCAGTTT200.00693653452.521
CCGCAGT200.00693653452.548
TGGCGTA350.001251156840.024
GGGTACA350.001251156840.062
AGGAGTG400.002410601135.05
GAATATG400.002410601135.01
CCTTTAA400.002410601135.01
TTGCCGC400.002410601135.045
TAGTGAA400.002410601135.09
AAATGGA400.002410601135.014