##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780182_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117107 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.72979411990743 34.0 34.0 34.0 34.0 34.0 2 33.72243333020229 34.0 34.0 34.0 34.0 34.0 3 33.72861571041868 34.0 34.0 34.0 34.0 34.0 4 33.76061208979822 34.0 34.0 34.0 34.0 34.0 5 33.743055496255565 34.0 34.0 34.0 34.0 34.0 6 37.48751142117892 38.0 38.0 38.0 37.0 38.0 7 37.481952402503694 38.0 38.0 38.0 37.0 38.0 8 37.382761064667356 38.0 38.0 38.0 37.0 38.0 9 37.39516852109609 38.0 38.0 38.0 37.0 38.0 10-11 37.34506476982588 38.0 38.0 38.0 36.5 38.0 12-13 37.33781072011067 38.0 38.0 38.0 36.5 38.0 14-15 37.24604421597343 38.0 38.0 38.0 36.0 38.0 16-17 37.27307505102172 38.0 38.0 38.0 36.0 38.0 18-19 37.23797040313559 38.0 38.0 38.0 36.0 38.0 20-21 37.26085118737565 38.0 38.0 38.0 36.0 38.0 22-23 37.24102316684741 38.0 38.0 38.0 36.0 38.0 24-25 37.25571058946092 38.0 38.0 38.0 36.0 38.0 26-27 37.157099917169774 38.0 38.0 38.0 36.0 38.0 28-29 37.113447530890554 38.0 38.0 38.0 36.0 38.0 30-31 37.07821479501652 38.0 38.0 38.0 36.0 38.0 32-33 37.01647638484463 38.0 38.0 38.0 36.0 38.0 34-35 37.087992178093536 38.0 38.0 38.0 36.0 38.0 36-37 37.08150238670618 38.0 38.0 38.0 36.0 38.0 38-39 36.44632259386715 38.0 37.0 38.0 34.0 38.0 40-41 36.73170690052687 38.0 37.0 38.0 34.5 38.0 42-43 36.99343335581989 38.0 38.0 38.0 36.0 38.0 44-45 37.080900373163004 38.0 38.0 38.0 36.0 38.0 46-47 37.02026351968712 38.0 38.0 38.0 35.5 38.0 48-49 37.02431536970463 38.0 38.0 38.0 36.0 38.0 50-51 36.885237432433584 38.0 37.5 38.0 35.0 38.0 52-53 36.884114527739584 38.0 38.0 38.0 35.0 38.0 54-55 36.86192114903464 38.0 37.5 38.0 35.0 38.0 56-57 36.905855328887256 38.0 37.5 38.0 35.0 38.0 58-59 36.79383384426208 38.0 37.5 38.0 34.5 38.0 60-61 36.79840231583082 38.0 37.0 38.0 35.0 38.0 62-63 36.78194727898418 38.0 37.0 38.0 35.0 38.0 64-65 36.70757085400531 38.0 37.0 38.0 34.5 38.0 66-67 36.38220601671975 38.0 37.0 38.0 34.0 38.0 68-69 36.28942761747803 38.0 37.0 38.0 34.0 38.0 70-71 35.65348356631115 38.0 37.0 38.0 30.5 38.0 72-73 34.646280751791096 38.0 36.5 38.0 23.5 38.0 74-75 34.34753686799252 38.0 36.0 38.0 22.0 38.0 76 33.67042106791225 38.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.059195568672983256 1101 2 -0.03540427149845726 1101 3 0.03487889732272009 1101 4 0.05946355945107484 1101 5 0.052766461617395066 1101 6 0.016253485770448606 1101 7 0.03770470057095565 1101 8 0.03668146208888601 1101 9 0.014234793714777538 1101 10-11 0.12787963523921064 1101 12-13 0.2594799290612855 1101 14-15 0.2238180479991172 1101 16-17 0.16401226188133222 1101 18-19 0.12560401444519442 1101 20-21 0.08816407761371892 1101 22-23 0.06247699501795978 1101 24-25 0.0010112019775760928 1101 26-27 -0.04775116716997729 1101 28-29 -0.07683797950126348 1101 30-31 0.02490117305993067 1101 32-33 0.11802473725160922 1101 34-35 0.23566024520301454 1101 36-37 0.0032543208223643205 1101 38-39 0.18778399225844566 1101 40-41 -0.046240805121733786 1101 42-43 0.16672295927964598 1101 44-45 0.09367868216303776 1101 46-47 0.05033233508603985 1101 48-49 0.030488777481870954 1101 50-51 0.11966272444423964 1101 52-53 0.055385589943298896 1101 54-55 -0.1163758164526385 1101 56-57 -0.2537684377489171 1101 58-59 0.2581844360477987 1101 60-61 -0.3761065874458751 1101 62-63 -0.14802564851714806 1101 64-65 -0.3482563501123792 1101 66-67 -0.297986791331617 1101 68-69 -0.4409662771108813 1101 70-71 -0.04472468533469964 1101 72-73 0.14745482396005372 1101 74-75 0.12464951541780067 1101 76 -1.6304027705411528 1102 1 0.09241959171222902 1102 2 -0.0877681066080811 1102 3 -0.08030682507117604 1102 4 0.09506297926566276 1102 5 0.0498024709508158 1102 6 0.027804640885960907 1102 7 -0.07587289922723528 1102 8 0.019160283219406438 1102 9 -0.1460204037794881 1102 10-11 -0.015406190158614663 1102 12-13 0.03229845295189193 1102 14-15 0.031689440233883204 1102 16-17 0.020001204724657384 1102 18-19 0.1350635591257756 1102 20-21 0.024895489608418586 1102 22-23 -0.006845701582903985 1102 24-25 -0.12129545543975695 1102 26-27 0.07695190823310583 1102 28-29 -0.09689746952669509 1102 30-31 0.14636172675861303 1102 32-33 0.24148230593841902 1102 34-35 0.024386570064798718 1102 36-37 0.0658239535818197 1102 38-39 0.14563646540385378 1102 40-41 0.1132366075876945 1102 42-43 0.29880973483619044 1102 44-45 0.050464328880572396 1102 46-47 0.03363098342221349 1102 48-49 0.062020593083815356 1102 50-51 -0.13375322224597141 1102 52-53 0.14186159423162792 1102 54-55 0.37165260422381863 1102 56-57 0.2576776113145911 1102 58-59 0.11302246762166135 1102 60-61 0.3526987771670136 1102 62-63 -0.07531853704166025 1102 64-65 -0.03908689813533073 1102 66-67 0.3164053803696376 1102 68-69 0.22376118289626845 1102 70-71 -0.4148083497521 1102 72-73 -0.8251965813078641 1102 74-75 -0.5485127052628656 1102 76 -0.2401334888359159 1103 1 0.04432948029423045 1103 2 0.12283633132288685 1103 3 -0.011530168362611448 1103 4 8.461932896750568E-4 1103 5 0.08159323810035346 1103 6 -0.2126083384650741 1103 7 0.033177699198255084 1103 8 -0.21326742660529163 1103 9 0.002026493238716398 1103 10-11 0.04429074537677735 1103 12-13 0.13784962443790505 1103 14-15 0.023812206707020778 1103 16-17 0.03585431334311551 1103 18-19 -0.0017945661208287333 1103 20-21 0.09213615623750826 1103 22-23 0.11205496166418527 1103 24-25 0.11751577278864289 1103 26-27 -0.05776898311086143 1103 28-29 0.0688438453183835 1103 30-31 0.13565207115858868 1103 32-33 0.086934460082297 1103 34-35 -0.032571887744794026 1103 36-37 0.13218624056300854 1103 38-39 -0.17516469199050277 1103 40-41 0.2581927355774738 1103 42-43 -0.0854602236404105 1103 44-45 -0.22275871378894863 1103 46-47 -0.023224135605275364 1103 48-49 0.09845221099336499 1103 50-51 -0.16422407131498318 1103 52-53 -0.19936776149803848 1103 54-55 -0.19020974851841999 1103 56-57 -0.13942514501255943 1103 58-59 0.20242930301238005 1103 60-61 0.02250318503579507 1103 62-63 -0.28361664124473407 1103 64-65 0.007697386854239596 1103 66-67 -0.35669177687441334 1103 68-69 -0.272401190513726 1103 70-71 -0.10691984290355805 1103 72-73 -0.0772304764283902 1103 74-75 -0.2354807453958898 1103 76 -0.306561536860265 1104 1 0.056437083869276705 1104 2 -0.13585252527906277 1104 3 -0.11665058398362049 1104 4 -0.041024101255295875 1104 5 -0.1232938309790299 1104 6 -0.036374463591648976 1104 7 0.051211424377669346 1104 8 -0.3337534918542673 1104 9 0.1737343257575361 1104 10-11 0.07557679301469022 1104 12-13 -0.05821870647390881 1104 14-15 -0.07482733892763349 1104 16-17 0.04840219341179619 1104 18-19 0.08314797779674876 1104 20-21 0.03070877984513487 1104 22-23 0.032611471152144134 1104 24-25 -0.033164454394032816 1104 26-27 0.11222617384770928 1104 28-29 -0.010639270078080187 1104 30-31 0.04143730428499737 1104 32-33 -0.2897088719302019 1104 34-35 -0.12916414768403683 1104 36-37 -0.0026616439084392596 1104 38-39 0.05169217412141336 1104 40-41 0.1012297628305916 1104 42-43 -0.11815955882290297 1104 44-45 -0.024290875986807237 1104 46-47 -0.11583189327709675 1104 48-49 0.06409303342985595 1104 50-51 -0.11529614457699466 1104 52-53 0.16703033650750854 1104 54-55 -0.16421582433402904 1104 56-57 -0.20166263015322272 1104 58-59 0.2591104988033308 1104 60-61 0.0686793836885542 1104 62-63 -0.002825904662159928 1104 64-65 -0.34797557576628435 1104 66-67 -0.5836278927551888 1104 68-69 -0.42151403337904725 1104 70-71 -0.30730288345301915 1104 72-73 -0.14188067675419802 1104 74-75 0.11082753620495112 1104 76 -0.18985304392670344 1105 1 -0.055991842925323 1105 2 0.10052807173586586 1105 3 -0.06483821724587813 1105 4 0.14438812333602868 1105 5 0.14267098778429954 1105 6 0.1766141646954864 1105 7 0.2341627392319694 1105 8 0.07386910942504699 1105 9 0.2137574245492928 1105 10-11 0.31545330332031085 1105 12-13 0.10023900496888416 1105 14-15 0.03487415360967816 1105 16-17 0.07871935759090576 1105 18-19 0.17742214268658785 1105 20-21 0.1191194052893394 1105 22-23 0.2639591968095658 1105 24-25 0.1432347993373142 1105 26-27 0.2721009073231855 1105 28-29 0.21811428358218166 1105 30-31 0.05143641586992942 1105 32-33 0.23646827803425907 1105 34-35 0.13738702502384825 1105 36-37 0.30367275552297457 1105 38-39 0.09491800923157712 1105 40-41 0.2847940840814758 1105 42-43 0.21560909789351257 1105 44-45 0.1540717750437537 1105 46-47 0.14934223607614427 1105 48-49 0.1575809509849364 1105 50-51 -0.15925936419319697 1105 52-53 0.11479153053736013 1105 54-55 -0.16928423229423117 1105 56-57 0.02980947361365338 1105 58-59 0.3035934412340424 1105 60-61 0.40673464310575014 1105 62-63 -0.04835273479719149 1105 64-65 -0.1258229460576814 1105 66-67 -0.0665658813834753 1105 68-69 0.0640136851710622 1105 70-71 -0.010852101647763845 1105 72-73 0.4994261818101222 1105 74-75 0.6019744800571232 1105 76 0.4696974180491367 1106 1 0.04079672280037272 1106 2 -0.1850258204797015 1106 3 0.10966990935805399 1106 4 0.06287258298815601 1106 5 0.022322399247087787 1106 6 0.04876272471829424 1106 7 0.12764363458411765 1106 8 0.13043454997252724 1106 9 0.04065719643409693 1106 10-11 0.12767707007251516 1106 12-13 0.09974000297287233 1106 14-15 -0.026503091899300557 1106 16-17 0.1930531899262391 1106 18-19 0.17393803946446695 1106 20-21 0.03308611472760248 1106 22-23 0.04239339983209334 1106 24-25 0.06033809275049151 1106 26-27 -0.06357595324139709 1106 28-29 0.0810936819283512 1106 30-31 0.17808490460674165 1106 32-33 0.13305902511154244 1106 34-35 0.054784373184673996 1106 36-37 -0.14166870441429325 1106 38-39 0.14144103460938595 1106 40-41 0.12764409833867063 1106 42-43 0.06104322963003739 1106 44-45 -0.051026956065456375 1106 46-47 -0.1730130533450165 1106 48-49 -0.10480499141597477 1106 50-51 0.29869080689320526 1106 52-53 0.32287536287269347 1106 54-55 0.01902664594928183 1106 56-57 0.07895225947051188 1106 58-59 0.1949001136035804 1106 60-61 0.22710283529280417 1106 62-63 0.120817852599437 1106 64-65 0.10668525183224631 1106 66-67 -0.030432790422537437 1106 68-69 0.2771766449657562 1106 70-71 0.10255501269497813 1106 72-73 0.17704416013917523 1106 74-75 0.2484761196351144 1106 76 0.3202997847443143 1107 1 0.07534873678482512 1107 2 -0.05358683509643214 1107 3 -0.14558355886078544 1107 4 0.06183118751822292 1107 5 0.0390219194613195 1107 6 -0.14921399265440982 1107 7 -0.095159206937808 1107 8 -0.009205424736443035 1107 9 -0.09589578456044023 1107 10-11 0.1104196594279685 1107 12-13 0.07798227619041143 1107 14-15 0.12338347037986352 1107 16-17 0.16354078616233636 1107 18-19 0.05429843668245127 1107 20-21 0.13730004711128885 1107 22-23 0.06824232310356848 1107 24-25 0.1096556067907315 1107 26-27 -0.028559030565013188 1107 28-29 0.16577215107700738 1107 30-31 0.020704014213613675 1107 32-33 0.17196258445247992 1107 34-35 -0.022939061932667926 1107 36-37 0.10426799361821537 1107 38-39 -0.17598002389472356 1107 40-41 0.16238207579990416 1107 42-43 0.15233270037798974 1107 44-45 0.15866546034396123 1107 46-47 0.10689917189395004 1107 48-49 0.1452879903224087 1107 50-51 -0.0437702337584156 1107 52-53 -0.06533269306512324 1107 54-55 0.1793829519707799 1107 56-57 0.057436285207856486 1107 58-59 0.11376424868745261 1107 60-61 -0.17860438380936472 1107 62-63 -0.0544992151284589 1107 64-65 0.5841278820996649 1107 66-67 0.2874066859043083 1107 68-69 0.4704577845499429 1107 70-71 0.40264427516382995 1107 72-73 0.014242434398113346 1107 74-75 0.21228762705504778 1107 76 0.4625804408234586 1108 1 -0.07013549132123842 1108 2 0.133949521886926 1108 3 0.03550705681540478 1108 4 -0.0476525902303635 1108 5 -0.16999085943192682 1108 6 -0.22144007210497563 1108 7 -0.017932283066983246 1108 8 0.14103570415961997 1108 9 -0.1217068971310411 1108 10-11 -0.09630849354345372 1108 12-13 -0.24392010882769455 1108 14-15 -0.0821953327951519 1108 16-17 -0.16382218319458985 1108 18-19 -0.04586857087980434 1108 20-21 -0.027671524073340947 1108 22-23 -0.06234726988586203 1108 24-25 -0.22071912815513173 1108 26-27 -0.01729540256027917 1108 28-29 0.09828889874547286 1108 30-31 -0.11698809139521416 1108 32-33 -0.3521925261665686 1108 34-35 -0.019631749253228747 1108 36-37 -0.04560252860880354 1108 38-39 0.07859391917259018 1108 40-41 -0.30877158992226583 1108 42-43 0.020556227802877913 1108 44-45 0.06870186423955715 1108 46-47 0.20472995007412464 1108 48-49 -0.0013080577907800262 1108 50-51 0.082850584015695 1108 52-53 -0.013607261003429016 1108 54-55 -0.24635497751074098 1108 56-57 -0.14757957112811226 1108 58-59 -0.16471147892134042 1108 60-61 -0.030554056361957294 1108 62-63 -0.19107950073015445 1108 64-65 0.01736238698792647 1108 66-67 -0.0547195643067937 1108 68-69 0.09948520006100381 1108 70-71 0.4481960568905805 1108 72-73 -0.23981547406673087 1108 74-75 -0.22029479774462857 1108 76 -0.02802772941166154 1109 1 0.09301609358261231 1109 2 0.0023137860215811656 1109 3 0.11452686211919882 1109 4 -0.17108806714016112 1109 5 -0.03847980586649413 1109 6 -0.15156043847911604 1109 7 0.12285321542244532 1109 8 0.03696434752028921 1109 9 -0.08961559132372088 1109 10-11 -0.3386141569971528 1109 12-13 -0.018014963285082786 1109 14-15 -0.0389353701998445 1109 16-17 0.06512808774963474 1109 18-19 0.016410237924681326 1109 20-21 0.023980516400449403 1109 22-23 -0.15360032699994974 1109 24-25 0.04333400568652479 1109 26-27 0.05553344700572893 1109 28-29 -0.06531825610035469 1109 30-31 -0.07028977460626606 1109 32-33 0.1566071669231519 1109 34-35 0.03520448534131759 1109 36-37 0.16537910472932538 1109 38-39 -0.17998278441922366 1109 40-41 -0.14020591591852138 1109 42-43 0.0820773518617699 1109 44-45 -0.17221790749592714 1109 46-47 0.1350565217904247 1109 48-49 -0.08428412838014765 1109 50-51 0.17496682628298998 1109 52-53 0.3639978797437067 1109 54-55 0.043910212150215955 1109 56-57 0.09518645774063828 1109 58-59 0.14982359996419348 1109 60-61 0.1473100399311491 1109 62-63 -0.022063052257514926 1109 64-65 -0.06977135875609974 1109 66-67 -0.15079207185966226 1109 68-69 0.07770416136154523 1109 70-71 0.18746139041572718 1109 72-73 -0.1421412785073386 1109 74-75 -0.48284694851430743 1109 76 0.10164186249357954 1110 1 0.06979136509472994 1110 2 0.04344602839221068 1110 3 0.09416222503629257 1110 4 0.05624497133838702 1110 5 0.0829905366564816 1110 6 0.08198420744943036 1110 7 -0.003462574051241063 1110 8 0.02062939248563822 1110 9 0.07660387892051546 1110 10-11 -0.09062162665168216 1110 12-13 -0.048625804112646165 1110 14-15 -0.0386552581550319 1110 16-17 0.10339041022248097 1110 18-19 0.2143757399149564 1110 20-21 0.04937242755087823 1110 22-23 -0.14322143860248815 1110 24-25 0.10850182720108847 1110 26-27 -0.021256567245586666 1110 28-29 0.11371265450950574 1110 30-31 0.24634003521239833 1110 32-33 0.303027138420525 1110 34-35 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-0.023924236991632597 2115 52-53 -0.23452353795578773 2115 54-55 -0.16857076197459264 2115 56-57 -0.057172750782953585 2115 58-59 -0.1816071458501085 2115 60-61 0.030246547867655238 2115 62-63 0.20358382749895299 2115 64-65 -0.06256870600550002 2115 66-67 -0.1778468663802144 2115 68-69 0.1695665227444465 2115 70-71 -0.13648403250229535 2115 72-73 -0.22784469229294757 2115 74-75 -0.4117445349461377 2115 76 -0.07404850497761117 2116 1 -0.08363991628894496 2116 2 0.05127895512809033 2116 3 -0.11758935303588913 2116 4 0.024267813728087617 2116 5 -0.09127020353322024 2116 6 -0.03403281494442467 2116 7 -0.03545067150334802 2116 8 -0.0015347270211876207 2116 9 -0.286631688693177 2116 10-11 0.20588637584259573 2116 12-13 0.10230025349656557 2116 14-15 0.24777168011144113 2116 16-17 -0.22086103110166988 2116 18-19 0.05078681541080954 2116 20-21 0.09356938846282503 2116 22-23 0.21067697402646957 2116 24-25 0.04087172513236936 2116 26-27 0.1181224873332809 2116 28-29 0.04843461842990848 2116 30-31 0.035414415196868276 2116 32-33 0.12595485888568447 2116 34-35 -0.14394184928205078 2116 36-37 -0.06193594378714096 2116 38-39 -0.24774878363398045 2116 40-41 -0.22822543468009115 2116 42-43 -0.10616652048104669 2116 44-45 -0.004334122651016514 2116 46-47 0.09221620545187648 2116 48-49 -0.02573345251498438 2116 50-51 0.08302672868918393 2116 52-53 -0.07425440585839738 2116 54-55 -0.3548334077948212 2116 56-57 -0.1390498987572073 2116 58-59 -0.017235685469650264 2116 60-61 0.02803806730228331 2116 62-63 0.019947155830429608 2116 64-65 -0.020946410637861845 2116 66-67 0.15804185040182261 2116 68-69 0.28293720864405003 2116 70-71 0.16058383138269505 2116 72-73 0.16231214008035977 2116 74-75 0.8658826493319012 2116 76 0.7300562435581384 2117 1 0.1570782442644827 2117 2 0.10492948018656989 2117 3 0.15145956947444006 2117 4 -0.09462931462506674 2117 5 0.08947682279436009 2117 6 -0.014448880240799156 2117 7 0.13552927176047547 2117 8 -0.2845312929894348 2117 9 0.18135467471696387 2117 10-11 -0.06887349077761229 2117 12-13 -0.06290653493722687 2117 14-15 0.04719809727164659 2117 16-17 0.07827921674583393 2117 18-19 -0.19319740795056362 2117 20-21 0.0394958305073132 2117 22-23 0.19150363007583593 2117 24-25 -0.1714659048880378 2117 26-27 0.0942211286511565 2117 28-29 -0.29029031064987265 2117 30-31 0.33951907851245267 2117 32-33 -0.1772850754197961 2117 34-35 0.1204101638568531 2117 36-37 0.15985149462424886 2117 38-39 0.5579671373335202 2117 40-41 0.46370085805867944 2117 42-43 0.15663022465407295 2117 44-45 -0.011693650844911474 2117 46-47 0.10063331588834501 2117 48-49 -0.19298075256972425 2117 50-51 -0.07003652717106235 2117 52-53 -0.04894086382883245 2117 54-55 0.35815121371516057 2117 56-57 -0.05907801800270818 2117 58-59 0.3110129583366472 2117 60-61 -0.008485846498537342 2117 62-63 0.20544086010555418 2117 64-65 0.1527023725204515 2117 66-67 0.4344191957459671 2117 68-69 0.5978416529778983 2117 70-71 0.6540330425507648 2117 72-73 1.0256040825478863 2117 74-75 1.3016936282797928 2117 76 0.6092430250243197 2118 1 -0.034497133930614154 2118 2 -0.052712669004876034 2118 3 -0.14102869343635405 2118 4 -0.17955367560576718 2118 5 -0.13689250427919575 2118 6 0.11298982607114283 2118 7 0.12655691912614486 2118 8 0.17929238984832807 2118 9 0.1214955197873877 2118 10-11 0.12963981125681556 2118 12-13 0.09468344941332418 2118 14-15 -0.07253325379772946 2118 16-17 0.19422861685016102 2118 18-19 0.027785899300347694 2118 20-21 0.029271521691462965 2118 22-23 -0.0697378931375141 2118 24-25 -0.02968186732935152 2118 26-27 0.056062547534828866 2118 28-29 0.030184389402293732 2118 30-31 -0.07478712910361907 2118 32-33 0.1447024050183856 2118 34-35 -0.07908122894440339 2118 36-37 0.05717804652827141 2118 38-39 0.0514986970622644 2118 40-41 -0.14708422279682765 2118 42-43 -0.13485386506945218 2118 44-45 0.09444875917073858 2118 46-47 0.1900829768168819 2118 48-49 -0.07211481621082783 2118 50-51 0.0019509532671193597 2118 52-53 0.014791530537365816 2118 54-55 0.0772435454835474 2118 56-57 -0.04740613485195411 2118 58-59 -0.014991214850624601 2118 60-61 0.07746877008987241 2118 62-63 -0.09984083003529065 2118 64-65 -0.050988289973034284 2118 66-67 0.052911631844033025 2118 68-69 0.07505865871603845 2118 70-71 0.018678321632663142 2118 72-73 0.5261126897466326 2118 74-75 0.43064511497775726 2118 76 0.033916994768709685 2119 1 -0.2689247433582196 2119 2 -0.08697192826413414 2119 3 -0.23421051161817275 2119 4 0.04536214931005844 2119 5 -0.2856298780165645 2119 6 0.0012894893708121913 2119 7 -0.07941951185028273 2119 8 -0.36877158321564707 2119 9 -0.2951711468792766 2119 10-11 0.018781225398228685 2119 12-13 0.022587489817368578 2119 14-15 -1.0939137251781972 2119 16-17 -0.27260639998485203 2119 18-19 -0.15171746770302263 2119 20-21 0.043497110917051884 2119 22-23 -0.06788604128566078 2119 24-25 -0.32172732187481046 2119 26-27 0.0858184397907138 2119 28-29 -0.21194524022733674 2119 30-31 -0.17651271832920656 2119 32-33 -0.22335315053716442 2119 34-35 -0.3634246632878373 2119 36-37 -0.13680343495321523 2119 38-39 -0.003972683409116939 2119 40-41 0.02505382434122083 2119 42-43 -0.017345447561034177 2119 44-45 -0.07171285699087804 2119 46-47 -0.09876382452991805 2119 48-49 -0.1205575771535905 2119 50-51 -0.18049854168237545 2119 52-53 -0.08975392400809312 2119 54-55 -0.22515544441544222 2119 56-57 -0.029308491754314048 2119 58-59 -0.05598448084388963 2119 60-61 -0.06634436122325127 2119 62-63 0.2418258366313708 2119 64-65 0.08129285480811888 2119 66-67 -0.017458738526336504 2119 68-69 0.2101175812749645 2119 70-71 0.5709931364474699 2119 72-73 0.6876025887365316 2119 74-75 0.8445340038666416 2119 76 -0.16423115337943983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 0.0 20 0.0 21 1.0 22 3.0 23 13.0 24 18.0 25 56.0 26 102.0 27 193.0 28 367.0 29 585.0 30 996.0 31 1634.0 32 2468.0 33 4107.0 34 6680.0 35 10767.0 36 16001.0 37 73114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.31721417165498 17.623199296369986 12.742193037136978 33.31739349483806 2 19.100480756914617 18.82893422254861 36.665613498766085 25.404971521770687 3 18.14579828703664 19.105604276430956 32.578752764565735 30.16984467196666 4 14.944452509243684 22.760381531419984 32.78198570538055 29.513180253955785 5 15.071686577232787 31.251761209833738 38.02163833075734 15.65491388217613 6 28.678900492711794 37.32227791677696 17.019477913361285 16.97934367714996 7 27.020588009256493 25.517688951130165 22.22668158180126 25.235041457812084 8 23.715917921217347 36.290742654153895 16.396116372206613 23.597223052422144 9 22.20191790413895 15.738598034276347 18.273032355025745 43.78645170655896 10-11 26.287070798500515 26.04370362147438 23.6518739272631 24.017351652762002 12-13 24.951113084614924 19.252478502566028 25.54202566883278 30.25438274398627 14-15 22.27151237756923 18.991605967192395 20.89712826731109 37.83975338792728 16-17 27.241753268378492 25.207289060431915 19.878828763438563 27.67212890775103 18-19 28.96581758562682 23.91317342259643 20.607649414637898 26.513359577138857 20-21 24.596309358108396 22.04351575909211 20.43771935067929 32.922455532120196 22-23 23.36879947398533 25.758494368398132 20.378798876241387 30.493907281375154 24-25 27.292561503582196 26.23882432305498 20.985082019008257 25.483532154354567 26-27 29.441877940686723 26.626077006498335 20.763916759886257 23.16812829292869 28-29 24.10530540445917 27.0709692845005 22.25486093914113 26.568864371899203 30-31 23.755625197468984 27.854013850581094 24.051508449537604 24.338852502412323 32-33 29.853467341832683 22.43247628237424 23.81796135158445 23.89609502420863 34-35 26.52061789645367 23.87901662582083 26.298171757452586 23.30219372027291 36-37 28.14178486341551 25.811864363360005 23.106646058732615 22.939704714491874 38-39 23.375203873380755 24.251752670634545 26.444619023627965 25.928424432356735 40-41 22.990085989735885 20.164038016514812 27.562400198109422 29.283475795639884 42-43 26.74092923565628 20.202037452927666 26.742210115535364 26.314823195880688 44-45 22.239917340551806 24.694083188878547 28.985030783813095 24.080968686756556 46-47 25.422476880118182 21.879563134569242 26.72683955698635 25.971120428326234 48-49 25.223086578940627 20.500909424714152 23.911038622797953 30.36496537354727 50-51 23.463584585037616 20.97483497997558 26.88951130163013 28.672069133356676 52-53 19.937749237876474 20.756658440571442 33.70336529840232 25.60222702314977 54-55 20.471449187495196 21.91798953094179 29.378260906692173 28.232300374870846 56-57 23.8132647920278 23.018692307035447 24.908844048605122 28.25919885233163 58-59 21.413749818542016 22.90981751731323 29.54776401069108 26.12866865345368 60-61 23.978925256389456 26.1863082480125 25.38191568394716 24.452850811650883 62-63 20.382214555918946 32.83108610074547 22.22070414236553 24.565995200970054 64-65 19.956962436062746 34.56198177734892 21.140068484377537 24.3409873022108 66-67 19.832717087791508 34.18839181261581 20.93384682384486 25.04504427574782 68-69 19.642292945767544 34.67000264715175 21.250651113938535 24.43705329314217 70-71 20.154217937441825 33.48134611936093 21.40094101975117 24.963494923446078 72-73 20.253699608050756 30.9405073992161 22.20704142365529 26.598751569077848 74-75 20.583739656894977 30.069936041398037 23.05583782352891 26.290486478178078 76 20.816859794888433 30.186069150435074 22.900424398199938 26.09664665647656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 0.0 16 1.5 17 3.0 18 2.0 19 3.0 20 5.5 21 6.0 22 18.5 23 40.0 24 59.0 25 69.0 26 118.5 27 221.5 28 328.0 29 381.0 30 481.5 31 665.5 32 928.5 33 1108.0 34 1279.0 35 1655.0 36 2024.0 37 2188.0 38 2613.5 39 4888.5 40 8205.0 41 9141.5 42 8611.0 43 7948.5 44 6947.5 45 6312.0 46 6015.0 47 6007.0 48 5586.0 49 4915.0 50 4657.0 51 4225.0 52 3333.5 53 2704.0 54 2534.0 55 2483.0 56 2243.0 57 1891.0 58 1728.0 59 1745.0 60 1801.5 61 1889.0 62 1937.0 63 1981.0 64 2030.0 65 2137.5 66 2332.0 67 2424.0 68 2490.5 69 2481.0 70 2398.0 71 2391.0 72 2267.5 73 1924.0 74 1539.0 75 1374.0 76 1190.0 77 816.5 78 460.5 79 294.0 80 224.0 81 93.0 82 17.0 83 2.0 84 5.0 85 4.5 86 2.0 87 3.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 117107.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.96936989249148 #Duplication Level Percentage of deduplicated Percentage of total 1 61.41148026178666 25.15989650490577 2 19.002459460586103 15.570375810156523 3 7.180374338238359 8.825262366895233 4 3.5620492725832675 5.837396568949764 5 2.1843344866397096 4.474540377603389 6 1.4902663720872067 3.6633164541829264 7 1.0921672433198548 3.1321782643223717 8 0.7503439076243278 2.4592893678430836 9 0.6419608987452583 2.367066016548968 >10 2.636625119846596 16.048570965014903 >50 0.025011463587477592 0.6250693809934504 >100 0.014590020426028595 1.2407456428736112 >500 0.0 0.0 >1k 0.006252865896869398 4.793906427455234 >5k 0.002084288632289799 5.802385852254775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 6795 5.802385852254775 No Hit CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG 1917 1.6369644854705525 RNA PCR Primer, Index 23 (95% over 24bp) CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 1850 1.5797518508714252 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 1847 1.5771900911132553 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 493 0.42098252025925015 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 271 0.23141229815467906 RNA PCR Primer, Index 47 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT 207 0.17676142331372163 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT 138 0.11784094887581442 No Hit CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 129 0.11015566960130478 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0025617597581698787 0.0 2 0.0 0.0 0.0 0.0025617597581698787 0.0 3 0.0 0.0 0.0 0.0025617597581698787 0.0 4 0.0 0.0 0.0 0.012808798790849394 0.0 5 0.0 0.0 0.0 0.01964015814596907 0.0 6 0.0 0.0 0.0 0.01964015814596907 0.0 7 0.0 0.0 0.0 0.02049407806535903 0.0 8 0.0 0.0 0.0 0.02305583782352891 0.0 9 0.0 0.0 0.0 0.02561759758169879 0.0 10 0.0 0.0 0.0 0.029887197178648586 0.0 11 0.0 0.0 0.0 0.029887197178648586 0.0 12 0.0 0.0 0.0 0.032448956936818464 0.0 13 0.0 0.0 0.0 0.03757247645315822 0.0 14 0.0 0.0 0.0 0.03757247645315822 0.0 15 0.0 0.0 0.0 0.04098815613071806 0.0 16 0.0 0.0 0.0 0.04098815613071806 0.0 17 0.0 0.0 0.0 0.04184207605010802 0.0 18 0.0 0.0 0.0 0.04269599596949798 0.0 19 0.0 0.0 0.0 0.04781951548583774 0.0 20 0.0 0.0 0.0 0.05123519516339758 0.0 21 0.0 0.0 0.0 0.05123519516339758 0.0 22 0.0 0.0 0.0 0.052943035002177494 0.0 23 0.0 0.0 0.0 0.057212634599127295 0.0 24 0.0 0.0 0.0 0.06148223419607709 0.0 25 0.0 0.0 0.0 0.06489791387363693 0.0 26 0.0 0.0 0.0 0.08026847242265621 0.0 27 0.0 0.0 0.0 0.13406542734422366 0.0 28 0.0 0.0 0.0 0.19981726113725054 0.0 29 0.0 0.0 0.0 0.28008573355990674 0.0 30 0.0 0.0 0.0 0.3697473250958525 0.0 31 0.0 0.0 0.0 0.46367851622874806 0.0 32 0.0 0.0 0.0 0.5627332268779833 0.0 33 0.0 0.0 0.0 0.7352250505947552 0.0 34 0.0 0.0 0.0 0.9743226280239439 0.0 35 0.0 0.0 0.0 1.2757563595685997 0.0 36 0.0 0.0 0.0 1.69161536031151 0.0 37 0.0 0.0 0.0 2.1663948354923277 0.0 38 0.0 0.0 0.0 2.7752397380173686 0.0 39 0.0 0.0 0.0 3.2730750510217153 0.0 40 0.0 0.0 0.0 3.850324916529328 0.0 41 0.0 0.0 0.0 4.473686457683998 0.0 42 0.0 0.0 0.0 5.122665596420368 0.0 43 0.0 0.0 0.0 5.7554202566883275 0.0 44 0.0 0.0 0.0 6.336085801873501 0.0 45 0.0 0.0 0.0 6.995311979642549 0.0 46 0.0 0.0 0.0 7.685279274509637 0.0 47 0.0 0.0 0.0 8.306079055906137 0.0 48 0.0 0.0 0.0 8.89528380028521 0.0 49 0.0 0.0 0.0 9.423006310468205 0.0 50 0.0 0.0 0.0 9.93962786169913 0.0 51 0.0 0.0 0.0 10.489552289786264 0.0 52 0.0 0.0 0.0 11.065948235374487 0.0 53 0.0 0.0 0.0 11.589401145960531 0.0 54 0.0 0.0 0.0 12.119685415901698 0.0 55 0.0 0.0 0.0 12.658508885036762 0.0 56 0.0 0.0 0.0 13.166591237073787 0.0 57 0.0 0.0 0.0 13.671257909433253 0.0 58 0.0 0.0 0.0 14.17336282203455 0.0 59 0.0 0.0 0.0 14.673759894797065 0.0 60 0.0 0.0 0.0 15.164763848446293 0.0 61 0.0 0.0 0.0 15.637835483788331 0.0 62 0.0 0.0 0.0 16.122008078082438 0.0 63 0.0 0.0 0.0 16.622405150844955 0.0 64 0.0 0.0 0.0 17.13475710247893 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAAAAG 15 0.0022199037 70.00001 3 TGTCTTC 15 0.0022199037 70.00001 48 TCTTTAT 80 0.0 65.625 1 CTTTATA 90 0.0 58.33333 2 TTTATAC 125 0.0 53.199997 3 TTGGACT 20 0.006936534 52.5 70 ATAGGAC 20 0.006936534 52.5 21 GGGTATC 20 0.006936534 52.5 9 TCTTAAA 20 0.006936534 52.5 57 GGCATAA 20 0.006936534 52.5 1 CCAGTTT 20 0.006936534 52.5 21 CCGCAGT 20 0.006936534 52.5 48 TGGCGTA 35 0.0012511568 40.0 24 GGGTACA 35 0.0012511568 40.0 62 AGGAGTG 40 0.0024106011 35.0 5 GAATATG 40 0.0024106011 35.0 1 CCTTTAA 40 0.0024106011 35.0 1 TTGCCGC 40 0.0024106011 35.0 45 TAGTGAA 40 0.0024106011 35.0 9 AAATGGA 40 0.0024106011 35.0 14 >>END_MODULE