FastQCFastQC Report
Wed 25 May 2016
SRR1780181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547345
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAG250.00234644635.21595812
TTCGGTA300.005757831529.31712226
CTATGGC407.049916E-427.48480430
TCTATGG407.049916E-427.48480429
TTATCAA407.0536835E-427.4822932
ACGAGAG508.323099E-526.40471824
AATTATC450.001401742524.43093730
CGGTAAG450.001401742524.43093728
TGGATTG450.001402489124.42870342
TACTGAT551.6039981E-423.9735872
TTATCGG651.8188008E-523.67921425
GGTAAGC500.002586938221.98784329
GGCTATA703.2249343E-521.98784330
ATTATCA703.2272106E-521.98583431
ATCGGAA755.4889235E-520.52198827
TATCGGA755.4889235E-520.52198826
TTTAGCG654.945816E-420.2964726
CTTACCG654.961081E-420.2871999
TACCGAA550.00447826220.00174511
ACTGATC550.004506786419.9798163
Produced by FastQC (version 0.11.5)