FastQCFastQC Report
Wed 25 May 2016
SRR1780181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547345
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT212013.873425353296367No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC60531.1058838575304424No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG56641.0348135088472536No Hit
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG40490.7397528067306726Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC12200.2228941526824946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG4500.037.64233444
GTCTTCT4500.037.64233443
CGTCTTC4800.035.2896942
CCATTTA505.9388185E-835.19802513
CCGTCTT5150.033.3185241
ATGCCGT28550.032.51656744
CTTTATA1400.029.8636552
ATAACAT300.005743875629.33168831
TCTTTAT1450.028.873451
TTAATAC804.294634E-927.5059992
TATGCCG34350.027.02614243
CGATAGA609.744495E-625.66522837
GCCGTCT7000.024.5129140
CTTAATA901.4833859E-824.4833341
GCCTTTC450.001397693924.44307339
AATCTCG450.001397693924.44307335
TTCCATA41400.022.68954829
CTTCCAT41700.022.5790728
GAGACTT41700.022.5790724
CGAGACT42000.022.41779323
Produced by FastQC (version 0.11.5)