FastQCFastQC Report
Wed 25 May 2016
SRR1780180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193213
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTTG252.3860439E-456.00000418
CTTAAAG200.0069433352.511
GATCGCA200.0069433352.527
ACGTTAC200.0069433352.520
GCTCTAT200.0069433352.544
GATTCTC400.002414471735.01
GTGAAAG450.00429968131.11110923
TTATACC1251.0903386E-725.22
AGTACGT700.001222863825.012
TATACCC1151.4870875E-624.3478263
ATACCCA1107.4463803E-419.0909084
GGTTTAG950.00718945118.42105136
GCACGAA5750.017.65217470
GATCTGC1200.001335602817.549
GTACGTG1250.00175544116.80000113
CCCATCT1503.1421383E-416.3333347
CTGGACG2151.085853E-616.279074
TGCACGA7850.015.60509570
ATGCACG8150.015.03067669
TACCCAT1450.00472100414.48275855
Produced by FastQC (version 0.11.5)