FastQCFastQC Report
Wed 25 May 2016
SRR1780180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193213
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT67593.498211818045369No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC18810.9735369773255422No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG16620.8601905668873212No Hit
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG16460.8519095505995974Illumina PCR Primer Index 9 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4100.21220104237292522No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT2410.12473280783384141Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT2390.12369768079787594No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG150.002222096570.057
CAGCTTA150.002222096570.011
TCTTTAT553.6379788E-1157.2727241
CTAAGTC200.0069433352.526
ATTGGGG200.0069433352.560
CTTTATA608.367351E-1152.52
TTTATAC751.4551915E-1146.6666683
CAGCTAG305.8672007E-446.66666419
TGCTACA305.8672007E-446.66666422
GGATTGT305.8672007E-446.66666413
AGAGATA305.8672007E-446.66666433
GTCTACA350.00125317840.01
TCTCGTA13950.035.6272438
CGTATGC14350.035.60975341
TATGCCG14350.035.60975343
TCGTATG14350.035.60975340
CTCGTAT14300.035.4895139
CTTGAAA14250.035.36842357
TCTGCTT14250.035.36842353
GTATGCC14450.035.3633242