##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780180_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193213 Sequences flagged as poor quality 0 Sequence length 76 %GC 58 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.73881157065001 34.0 34.0 34.0 34.0 34.0 2 33.72196487813967 34.0 34.0 34.0 34.0 34.0 3 33.715583319962946 34.0 34.0 34.0 34.0 34.0 4 33.760518184594204 34.0 34.0 34.0 34.0 34.0 5 33.73965519918432 34.0 34.0 34.0 34.0 34.0 6 37.46822936344863 38.0 38.0 38.0 37.0 38.0 7 37.45652725230704 38.0 38.0 38.0 37.0 38.0 8 37.353159466495526 38.0 38.0 38.0 37.0 38.0 9 37.381465015294005 38.0 38.0 38.0 37.0 38.0 10-11 37.33423475646049 38.0 38.0 38.0 36.0 38.0 12-13 37.31337694668578 38.0 38.0 38.0 36.0 38.0 14-15 37.23079709957405 38.0 38.0 38.0 36.0 38.0 16-17 37.28050907547629 38.0 38.0 38.0 36.0 38.0 18-19 37.2338119070663 38.0 38.0 38.0 36.0 38.0 20-21 37.25748785019641 38.0 38.0 38.0 36.0 38.0 22-23 37.21492860211269 38.0 38.0 38.0 36.0 38.0 24-25 37.233488429867556 38.0 38.0 38.0 36.0 38.0 26-27 37.144024987966645 38.0 38.0 38.0 36.0 38.0 28-29 37.10142433480149 38.0 38.0 38.0 36.0 38.0 30-31 37.085985415060065 38.0 38.0 38.0 36.0 38.0 32-33 37.058073214535256 38.0 38.0 38.0 36.0 38.0 34-35 37.0755332198144 38.0 38.0 38.0 36.0 38.0 36-37 37.080672625547976 38.0 38.0 38.0 36.0 38.0 38-39 36.67561965292191 38.0 37.5 38.0 34.0 38.0 40-41 36.84221817372537 38.0 37.5 38.0 34.5 38.0 42-43 36.97137097400278 38.0 38.0 38.0 35.5 38.0 44-45 37.03000833277264 38.0 38.0 38.0 36.0 38.0 46-47 36.98448344573087 38.0 38.0 38.0 35.5 38.0 48-49 37.009383426581024 38.0 38.0 38.0 36.0 38.0 50-51 36.87671119438133 38.0 38.0 38.0 35.0 38.0 52-53 36.88950795236345 38.0 38.0 38.0 35.0 38.0 54-55 36.86453033698561 38.0 37.5 38.0 35.0 38.0 56-57 36.9116130902165 38.0 38.0 38.0 35.0 38.0 58-59 36.81010594525213 38.0 37.5 38.0 34.5 38.0 60-61 36.82811198004275 38.0 37.5 38.0 35.0 38.0 62-63 36.783945697235694 38.0 37.0 38.0 34.5 38.0 64-65 36.74616097260537 38.0 37.5 38.0 34.5 38.0 66-67 36.522889246582785 38.0 37.0 38.0 34.0 38.0 68-69 36.49407648553669 38.0 37.0 38.0 34.0 38.0 70-71 36.07829442118285 38.0 37.0 38.0 32.5 38.0 72-73 35.48536589152904 38.0 37.0 38.0 29.0 38.0 74-75 35.31121353118061 38.0 37.0 38.0 27.0 38.0 76 34.467468545077196 38.0 35.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1062457827201726 1101 2 0.034444159242454475 1101 3 -0.012555065912188468 1101 4 0.08745997151960694 1101 5 0.047653338707185355 1101 6 0.04029081070100915 1101 7 0.14499382853518483 1101 8 0.17411790843066655 1101 9 0.21074668935154506 1101 10-11 0.2432953617758784 1101 12-13 0.2336048875544776 1101 14-15 0.3313714839990851 1101 16-17 0.1251059870446838 1101 18-19 0.13491965852037424 1101 20-21 0.09237025343060168 1101 22-23 0.08613031290767736 1101 24-25 0.15757729662063014 1101 26-27 0.06598269749769514 1101 28-29 0.07872896116397499 1101 30-31 0.1923670748995434 1101 32-33 0.19804122332494956 1101 34-35 0.25891954816513874 1101 36-37 0.3243802961118618 1101 38-39 0.24677665793996795 1101 40-41 0.20662216509593634 1101 42-43 0.2899085159775865 1101 44-45 0.11876508756066784 1101 46-47 0.3171428418455662 1101 48-49 0.19252659946985773 1101 50-51 0.1564597843176827 1101 52-53 0.15457391644289942 1101 54-55 0.13156476533870176 1101 56-57 0.14694095538927598 1101 58-59 0.1586175334048434 1101 60-61 -0.14031236192486318 1101 62-63 0.07960952213035455 1101 64-65 0.1932252730502242 1101 66-67 0.18018540540060712 1101 68-69 -0.34101362216276954 1101 70-71 -0.006254250289138952 1101 72-73 -0.1834308664999611 1101 74-75 -0.1300814970991908 1101 76 -0.9329441504681384 1102 1 0.061906160078663675 1102 2 0.003425291317931567 1102 3 -0.09036638666690067 1102 4 -0.10004946244265511 1102 5 -0.06332779336828054 1102 6 0.0743662823991258 1102 7 -0.06168541674783512 1102 8 0.015702814091049788 1102 9 -0.022762744610716368 1102 10-11 -0.05632727973355145 1102 12-13 0.001303000762021611 1102 14-15 0.11178657833870886 1102 16-17 -0.08976193748362249 1102 18-19 -0.0024576999701935165 1102 20-21 0.09608723456267398 1102 22-23 0.13956427517182846 1102 24-25 0.009275409828177317 1102 26-27 0.11962420693165399 1102 28-29 0.20287990456020566 1102 30-31 -0.06380273642120926 1102 32-33 0.1148902799287157 1102 34-35 0.1274855859009847 1102 36-37 0.13534256026280644 1102 38-39 0.13804080888336046 1102 40-41 0.2551882028317891 1102 42-43 0.14815379899645365 1102 44-45 0.19548206869274765 1102 46-47 0.04833152109085148 1102 48-49 0.12535678814910511 1102 50-51 -0.008030781720052005 1102 52-53 0.062385237197617016 1102 54-55 -0.11605787617072849 1102 56-57 0.04046925727606521 1102 58-59 0.2600326277444651 1102 60-61 0.21674424184871555 1102 62-63 0.19160952213034932 1102 64-65 0.18671583908795952 1102 66-67 0.34143068841947155 1102 68-69 -0.12118343348352312 1102 70-71 0.1535570704655811 1102 72-73 0.460437058028333 1102 74-75 0.4729185029008036 1102 76 0.4088671702865767 1103 1 0.10107336892706797 1103 2 0.05081197533441184 1103 3 0.09170547048628208 1103 4 0.02498487573416952 1103 5 0.06974529273017538 1103 6 -0.04200037703845538 1103 7 0.11320072508690515 1103 8 0.13828649080615207 1103 9 -0.0038586746331290556 1103 10-11 -0.02551689876052876 1103 12-13 0.11542864234374406 1103 14-15 0.08134849549333723 1103 16-17 -0.04675608192084013 1103 18-19 0.07305375813722748 1103 20-21 0.09386067488654248 1103 22-23 0.12687360792683222 1103 24-25 -0.045947607593930684 1103 26-27 0.20617809979654567 1103 28-29 0.05347991901838611 1103 30-31 0.005014584478090001 1103 32-33 0.1301906486122988 1103 34-35 0.07657472057893244 1103 36-37 -0.03635916748967105 1103 38-39 -0.055449395699881165 1103 40-41 0.19431565168596876 1103 42-43 0.05722269222279408 1103 44-45 0.11030531744572869 1103 46-47 -0.04986482098968281 1103 48-49 0.02712430061927762 1103 50-51 -0.12272029231066739 1103 52-53 -0.0396099916030721 1103 54-55 -0.1302666522858189 1103 56-57 -0.16298624767586034 1103 58-59 0.13955623072284595 1103 60-61 -0.18192922016240942 1103 62-63 -0.12065101426811964 1103 64-65 0.08454711213067867 1103 66-67 -0.026320341725835306 1103 68-69 -0.04795998231602994 1103 70-71 -0.07506651082553617 1103 72-73 0.31340821395980356 1103 74-75 0.1863284645866301 1103 76 0.33407500661998313 1104 1 -0.09841600887032342 1104 2 0.019533738767137265 1104 3 -0.09349839315167685 1104 4 -0.05104459886430135 1104 5 0.0708379822172418 1104 6 0.07793980521655186 1104 7 0.040704980271925706 1104 8 -0.3121782524468415 1104 9 0.11600445900419487 1104 10-11 0.006878542763075757 1104 12-13 -0.1211263738714834 1104 14-15 -0.04552431124771772 1104 16-17 -0.10727426889680913 1104 18-19 0.08515914663737334 1104 20-21 0.11522765745254304 1104 22-23 0.04886888694790059 1104 24-25 0.02733963665718875 1104 26-27 0.0499077432015298 1104 28-29 -0.11401326918337418 1104 30-31 -0.12943000005474659 1104 32-33 -0.15779607100778037 1104 34-35 -0.16518282843449583 1104 36-37 -0.18362884465596352 1104 38-39 -0.24734948465601292 1104 40-41 -0.12239794459327413 1104 42-43 -0.20686844928749792 1104 44-45 -0.1926901226769857 1104 46-47 -0.1875244342056206 1104 48-49 0.07490319910422727 1104 50-51 -0.1591380218980376 1104 52-53 -0.20084473072346043 1104 54-55 -0.2274407191219865 1104 56-57 -0.4422857356527743 1104 58-59 -0.08512104063537862 1104 60-61 -0.09008201457568532 1104 62-63 -0.00583106287879076 1104 64-65 -0.3110800285951214 1104 66-67 -0.47108698948056826 1104 68-69 -0.35033903349732043 1104 70-71 -0.11259794315933647 1104 72-73 0.0025380548894347044 1104 74-75 -0.022693928543439768 1104 76 0.33247449669822515 1105 1 -0.016816947907138058 1105 2 0.0023113179140423767 1105 3 0.13737851342360585 1105 4 0.07171827410263631 1105 5 0.1189669918437204 1105 6 0.06996608745377841 1105 7 0.015923717834077422 1105 8 0.046176949021074165 1105 9 0.1556470583552354 1105 10-11 0.05598908871314734 1105 12-13 -0.010432012814526104 1105 14-15 0.004928679571037264 1105 16-17 0.10825727855759482 1105 18-19 0.12231080243180514 1105 20-21 0.1620344600726753 1105 22-23 0.011680128405835433 1105 24-25 -0.19415702072254248 1105 26-27 0.08314505912130699 1105 28-29 0.04647803865475453 1105 30-31 -0.012389382665027426 1105 32-33 0.0729526624393344 1105 34-35 0.002233201301663712 1105 36-37 0.06743195662846091 1105 38-39 0.020426104434434933 1105 40-41 0.05115353040959292 1105 42-43 -0.08533502645784097 1105 44-45 -0.228054100631212 1105 46-47 -0.3314881544644024 1105 48-49 -0.05145864038254189 1105 50-51 -0.14585017878194861 1105 52-53 -0.020894718243440025 1105 54-55 -0.24451641547310032 1105 56-57 0.03918818786159761 1105 58-59 0.07291642639377471 1105 60-61 0.08650682626701922 1105 62-63 -0.001194905913934008 1105 64-65 -0.09280793728188286 1105 66-67 -0.14021311858464003 1105 68-69 -0.04615384356497998 1105 70-71 -0.1651472392190243 1105 72-73 -0.010275884950146974 1105 74-75 0.13970817079748343 1105 76 -0.1380659216858504 1106 1 0.06227214806857262 1106 2 0.06798841536298283 1106 3 0.10995340866408299 1106 4 0.10398351200925049 1106 5 0.03143111648496699 1106 6 -0.007615027340420966 1106 7 -0.06057679293374463 1106 8 -0.0019498881794959289 1106 9 -0.07993504322848821 1106 10-11 0.04016918475139164 1106 12-13 0.044953286801174386 1106 14-15 -0.022538120520685823 1106 16-17 -0.08445333498921315 1106 18-19 -0.022682978014465505 1106 20-21 -0.0834885036315427 1106 22-23 -0.0814516079962786 1106 24-25 0.07467710829671148 1106 26-27 -0.005183027549392705 1106 28-29 0.1269587163428838 1106 30-31 0.08110718789389892 1106 32-33 0.058984726149802214 1106 34-35 0.060177551931616335 1106 36-37 -0.061896540046333826 1106 38-39 0.03952430389099959 1106 40-41 0.01867112931439152 1106 42-43 0.0019593634352190747 1106 44-45 0.11402309132714805 1106 46-47 0.03791685314499915 1106 48-49 0.01395786124010101 1106 50-51 0.17013963365855034 1106 52-53 0.23852495222444503 1106 54-55 0.3815685318170452 1106 56-57 0.13467918514444932 1106 58-59 0.12241226033516739 1106 60-61 0.23642586783032016 1106 62-63 0.023059616292307794 1106 64-65 0.36946821090332804 1106 66-67 0.19476544306538557 1106 68-69 0.13323684864703011 1106 70-71 0.27263463859974735 1106 72-73 -0.06021994371276662 1106 74-75 0.19457575337161614 1106 76 0.27540236553939934 1107 1 6.487677948001647E-4 1107 2 -0.15572001986202366 1107 3 -0.03025655844950137 1107 4 0.02317638943005562 1107 5 -0.05791382547191404 1107 6 -0.10509724900047956 1107 7 -0.04017035056929785 1107 8 -0.02392686768873631 1107 9 0.008672062485871379 1107 10-11 -0.06390613076143836 1107 12-13 0.08911981292760629 1107 14-15 0.12119237952147444 1107 16-17 0.10503882286557342 1107 18-19 -0.2887221325244056 1107 20-21 0.111706074326122 1107 22-23 0.0907646412658849 1107 24-25 0.005301177217646114 1107 26-27 0.009296130333510177 1107 28-29 0.116340901462479 1107 30-31 -0.1427523280855283 1107 32-33 0.09788450690703598 1107 34-35 -0.09535657123784347 1107 36-37 0.015186266261117964 1107 38-39 -0.20127558086600317 1107 40-41 0.17208485166310084 1107 42-43 -0.09956163327614576 1107 44-45 0.11427255024723593 1107 46-47 -0.06954372531861708 1107 48-49 -0.04426444530626128 1107 50-51 -0.004950663443509029 1107 52-53 0.08324555823394064 1107 54-55 0.12169909369691112 1107 56-57 -0.23723068640176592 1107 58-59 -0.06451679495337004 1107 60-61 -0.2474243022233722 1107 62-63 0.10392295496616555 1107 64-65 -0.06655084635276154 1107 66-67 0.11423764420656823 1107 68-69 0.20639682559842498 1107 70-71 0.0889696303078793 1107 72-73 0.02701689469406432 1107 74-75 0.05538118946797255 1107 76 -0.5896754937517201 1108 1 -0.1157168341022583 1108 2 -0.025705373467822312 1108 3 0.12056642941491447 1108 4 -0.03704470137758875 1108 5 -0.06971114727411987 1108 6 -0.037148441635437734 1108 7 0.16437700610527628 1108 8 -0.08685405418615488 1108 9 0.018746689351544887 1108 10-11 -0.03352706813066675 1108 12-13 -0.15964744889411975 1108 14-15 -0.00902103936540044 1108 16-17 0.07957327676430737 1108 18-19 0.19156451833345756 1108 20-21 0.15632352445864228 1108 22-23 -0.10772015438204363 1108 24-25 0.11795112839632793 1108 26-27 0.10492662273134101 1108 28-29 0.16910279293968244 1108 30-31 0.13476894405842899 1108 32-33 -0.2055382159273904 1108 34-35 -0.054201947161963915 1108 36-37 0.04717468863522356 1108 38-39 0.13418787289323575 1108 40-41 -0.01575166667976191 1108 42-43 0.07498328233272389 1108 44-45 0.17440994737375348 1108 46-47 0.2022549913782754 1108 48-49 0.07813407610537126 1108 50-51 -0.10795143063558754 1108 52-53 -0.0018186069215886391 1108 54-55 0.0020694382358996677 1108 56-57 0.1715484320247853 1108 58-59 0.11539323433942172 1108 60-61 0.1078745539629864 1108 62-63 0.22431980250418349 1108 64-65 0.294580413237135 1108 66-67 0.21919475119499765 1108 68-69 0.15168731241666933 1108 70-71 0.3603812637295505 1108 72-73 0.38502707742527065 1108 74-75 0.15048859635874834 1108 76 0.7054109897187786 1109 1 0.026958692161407782 1109 2 0.04559637504207359 1109 3 -0.06737587516074228 1109 4 0.01409966323444678 1109 5 0.11492501500776342 1109 6 -0.09932768640881307 1109 7 -0.024065901715296434 1109 8 -0.009134500047174754 1109 9 0.0985154754787132 1109 10-11 -0.19146379043992567 1109 12-13 -0.06413307005631452 1109 14-15 0.11677803505881457 1109 16-17 0.014269763537939184 1109 18-19 0.025742394551194536 1109 20-21 -0.08040238626111318 1109 22-23 -0.1794072593466538 1109 24-25 -0.013867790084574949 1109 26-27 -0.12411556839825977 1109 28-29 -0.01974888083987736 1109 30-31 0.03750965678779039 1109 32-33 -0.08540964372707549 1109 34-35 -0.024448467249115424 1109 36-37 0.012170276844038597 1109 38-39 -0.253221415277757 1109 40-41 -0.07140866342044205 1109 42-43 -0.020590520631273534 1109 44-45 0.1055049719537351 1109 46-47 0.06727578982244609 1109 48-49 -0.004352013246901265 1109 50-51 -0.05175601529695939 1109 52-53 0.1002810455373151 1109 54-55 -0.17701326934336947 1109 56-57 -0.19899160723115017 1109 58-59 0.10847302473432308 1109 60-61 0.010654882776485408 1109 62-63 0.020125899779671386 1109 64-65 -0.056938503443035415 1109 66-67 -0.11295910326991532 1109 68-69 -0.11264560674851509 1109 70-71 0.4039769635836947 1109 72-73 -0.5411861651512169 1109 74-75 -0.142110398833303 1109 76 0.07855488614072925 1110 1 -0.054822919569907924 1110 2 -0.14294515373464378 1110 3 -0.14421918804958977 1110 4 -9.064406941305947E-4 1110 5 0.13724112496672802 1110 6 -0.07030460914631931 1110 7 0.01172665296266473 1110 8 0.18028584736196507 1110 9 0.011876460343913209 1110 10-11 -0.06479624127756267 1110 12-13 -0.012189005575294232 1110 14-15 -0.20699492821465526 1110 16-17 -0.11345126486371271 1110 18-19 0.014599047833350198 1110 20-21 -0.0019503572564190108 1110 22-23 0.033947106800816584 1110 24-25 -0.04009445910456577 1110 26-27 0.032097201314485346 1110 28-29 0.07288926650748806 1110 30-31 0.018863258105653813 1110 32-33 -0.0413786240033005 1110 34-35 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2115 54-55 -0.27623280551149776 2115 56-57 -0.07778374096699991 2115 58-59 -0.004641575906902062 2115 60-61 -0.075227341681952 2115 62-63 0.031690493790264895 2115 64-65 0.10575563742269622 2115 66-67 0.3144121260483814 2115 68-69 0.21485277459836283 2115 70-71 -0.5241489871312481 2115 72-73 0.20209544928950862 2115 74-75 0.1658334826578951 2115 76 0.2920922867788249 2116 1 0.001587397627005771 2116 2 -0.11107136870785439 2116 3 0.05998323636524816 2116 4 0.07582850154031462 2116 5 -0.10659510849777831 2116 6 0.14270488937596326 2116 7 0.15432302108176543 2116 8 0.2549543473540652 2116 9 0.12806345953788423 2116 10-11 0.1646618214032003 2116 12-13 0.11024670950064319 2116 14-15 0.1859926020115097 2116 16-17 -0.21292299846256668 2116 18-19 0.0840066150421066 2116 20-21 0.04571807951755602 2116 22-23 0.06273900855985204 2116 24-25 0.13716321794568387 2116 26-27 0.06644439522025891 2116 28-29 0.22723827793416262 2116 30-31 0.13284947655586166 2116 32-33 -0.1268593977930692 2116 34-35 0.23371043843428652 2116 36-37 0.11980679714705644 2116 38-39 0.16739156477226658 2116 40-41 -0.22564698604277567 2116 42-43 0.18039919920740033 2116 44-45 0.1498748184095362 2116 46-47 0.21068942569649352 2116 48-49 0.17024916675764246 2116 50-51 0.16671237230939795 2116 52-53 0.007687788078513336 2116 54-55 0.1245999620260747 2116 56-57 0.20258691812219354 2116 58-59 -0.1321174562431935 2116 60-61 -0.0010494219662717796 2116 62-63 -0.1582869582009181 2116 64-65 0.07501616331936845 2116 66-67 0.0035932728954293225 2116 68-69 -0.10889975052715783 2116 70-71 0.17153042879989044 2116 72-73 0.22655878153316422 2116 74-75 0.29921249876002065 2116 76 0.3879171331757547 2117 1 0.032144302804738345 2117 2 -0.003196432723719056 2117 3 0.07225677341128289 2117 4 0.011015996889589985 2117 5 0.0570360025549661 2117 6 0.026434573914542625 2117 7 0.10838917737239484 2117 8 -0.3769095757130927 2117 9 -0.14658100620870584 2117 10-11 -0.06511901454547342 2117 12-13 0.056983323072444136 2117 14-15 0.14055118801599775 2117 16-17 0.12022649444426037 2117 18-19 0.12472726951403246 2117 20-21 0.12403410121072511 2117 22-23 0.15010917125862733 2117 24-25 -0.16411403530325686 2117 26-27 -0.03779531518729584 2117 28-29 -0.7666621593434257 2117 30-31 0.05989984445556473 2117 32-33 -0.015990489148627773 2117 34-35 0.14273350165350962 2117 36-37 0.07791306566788592 2117 38-39 0.047763972950541245 2117 40-41 -0.3036653613311273 2117 42-43 -0.11639803793362091 2117 44-45 -0.010609119627488894 2117 46-47 0.10905193275466019 2117 48-49 -0.10464041955762582 2117 50-51 0.3055337800893554 2117 52-53 0.11688681284881852 2117 54-55 0.13307850741058758 2117 56-57 -0.09156433002298314 2117 58-59 0.21533000697777993 2117 60-61 -0.07227007862677226 2117 62-63 0.04361797878996754 2117 64-65 -0.2104639447087635 2117 66-67 0.06789365064373243 2117 68-69 -0.01132863273359419 2117 70-71 0.5127098089074806 2117 72-73 -0.03802917516803461 2117 74-75 0.066153175205244 2117 76 -0.5751090553307137 2118 1 0.06174261147281612 2118 2 -0.17444379001758392 2118 3 -0.20892715893544533 2118 4 -0.10949140268758129 2118 5 -0.14169549850211638 2118 6 -0.1469269482841895 2118 7 -0.04171653087285421 2118 8 -0.0027023875524179175 2118 9 -0.10852603792118032 2118 10-11 -0.018607386638500145 2118 12-13 -0.005384541620998107 2118 14-15 -0.03069616927787422 2118 16-17 0.04305947541677568 2118 18-19 -0.16279915754728336 2118 20-21 0.009869196348148535 2118 22-23 -0.04756524734602152 2118 24-25 -0.06897661458444304 2118 26-27 -0.027224490662987932 2118 28-29 0.10543086391238887 2118 30-31 0.005988639381577343 2118 32-33 0.07174735440316482 2118 34-35 0.04125358622010822 2118 36-37 0.22061919674610664 2118 38-39 0.17038553743256557 2118 40-41 -0.031995171056287575 2118 42-43 0.2017837802481992 2118 44-45 0.11624077466426286 2118 46-47 0.04562698560878431 2118 48-49 0.1490593690258848 2118 50-51 0.17762680334517 2118 52-53 0.02914896929702593 2118 54-55 0.30749711206174624 2118 56-57 0.1600212936556531 2118 58-59 0.13816298795029525 2118 60-61 -0.06804174881703773 2118 62-63 0.23917823248975623 2118 64-65 -0.1628952343493566 2118 66-67 -0.14035159248523144 2118 68-69 -0.16883391814585025 2118 70-71 -0.012660169950869715 2118 72-73 0.054994080645911936 2118 74-75 0.07249566991983869 2118 76 -0.006398695922683828 2119 1 0.06711534793755902 2119 2 0.07992242011201967 2119 3 -0.018381152868705897 2119 4 0.002329536737001092 2119 5 -0.16373796564063525 2119 6 -0.042491797994642866 2119 7 0.04003730679605155 2119 8 0.11385703886545429 2119 9 0.08613799369937425 2119 10-11 0.06916492699326682 2119 12-13 0.08612662668491566 2119 14-15 -0.8189763420878649 2119 16-17 -0.2295896651292324 2119 18-19 0.03618052808558758 2119 20-21 0.17289199256103416 2119 22-23 0.042086834646809734 2119 24-25 -0.21785748598806265 2119 26-27 -0.11932165032838782 2119 28-29 0.102859395946588 2119 30-31 -0.1057633598830634 2119 32-33 -0.17313268971853546 2119 34-35 -0.1391674083565988 2119 36-37 -0.02470666040988334 2119 38-39 0.0572983970703973 2119 40-41 0.24505694770462583 2119 42-43 0.0569519942384602 2119 44-45 0.07446073973458311 2119 46-47 0.24966458097600253 2119 48-49 0.16274399077420298 2119 50-51 0.10915543649159787 2119 52-53 -0.09655651833971035 2119 54-55 0.034434330556102566 2119 56-57 -0.057972088088988016 2119 58-59 0.08687840297005778 2119 60-61 0.0926876380751338 2119 62-63 0.020957348217308436 2119 64-65 -0.3489486399932602 2119 66-67 -0.10303198590374052 2119 68-69 -0.4577092743366791 2119 70-71 -0.011906424202187793 2119 72-73 -0.0584308664999611 2119 74-75 0.00661415507472185 2119 76 -1.7544224113376998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 1.0 20 3.0 21 3.0 22 10.0 23 21.0 24 45.0 25 107.0 26 216.0 27 339.0 28 552.0 29 959.0 30 1560.0 31 2426.0 32 3708.0 33 6140.0 34 9425.0 35 15763.0 36 25742.0 37 126191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.709859067454055 14.76246422342182 10.382324170733852 32.14535253839028 2 23.485479755502993 16.738521735079935 30.701350323218417 29.074648186198655 3 22.613902791220053 16.121068458126523 26.391598908976103 34.87342984167732 4 18.705877481729914 17.533072479970187 26.08067821874418 37.68037181955572 5 21.066387872451646 26.742506974168407 30.706525958398245 21.484579194981706 6 37.064468045463016 29.058754114651265 13.697906962300479 20.178870877585243 7 33.675787861065245 21.74957171618887 16.983329279085776 27.591311143660107 8 28.840709476070455 30.110810349200108 14.979840900974573 26.068639273754872 9 25.497766713419907 13.571033005025543 15.428050907547627 45.503149374006924 10-11 31.902356466697373 20.917329579272618 19.619539057930886 27.560774896099122 12-13 29.68925486380316 16.11459891415174 21.260732973454168 32.93541324859093 14-15 27.198221651752213 16.139959526532895 17.536863461568323 39.12495536014657 16-17 31.2688587206865 20.55451755316671 17.097452034800973 31.07917169134582 18-19 32.42380171106499 19.284157898278064 17.394015930605082 30.89802446005186 20-21 29.422450870283058 18.72855346172359 17.46466335080973 34.38433231718363 22-23 29.537867534793204 20.366900777897968 17.33087318141119 32.76435850589763 24-25 31.579396831476142 20.17514349448536 18.027772458374955 30.217687215663542 26-27 31.274551919384308 21.95918494097188 17.97006412611988 28.796199013523932 28-29 31.16560479884894 19.545786256618346 18.13076759845352 31.157841346079195 30-31 30.38149606910508 17.88181954630382 21.980922608727155 29.75576177586394 32-33 27.826802544342254 22.512719123454424 20.588935527112564 29.071542805090754 34-35 28.27087204277145 21.76302836765642 18.777204432413967 31.188895157158164 36-37 31.2476386164492 20.496809220911636 18.70241650406546 29.553135658573698 38-39 28.077820850563885 19.42622908396433 22.121958667377452 30.37399139809433 40-41 27.706469026411266 17.379782933860557 23.00750984664593 31.90623819308225 42-43 29.7570556846589 17.036897103196992 22.529539937788865 30.676507274355245 44-45 26.49847577543954 19.908857064483236 24.60186426379177 28.990802896285444 46-47 28.064622980855326 18.639014973112573 23.209877182177184 30.086484863854917 48-49 28.132682583470057 18.008622608209592 21.145575090703005 32.713119717617346 50-51 26.175516140218306 18.70785092100428 23.43527609425867 31.681356844518742 52-53 24.44090200969914 18.56422704476407 27.317778824406226 29.677092121130567 54-55 24.536133697007966 18.838535709294923 24.8860066351643 31.73932395853281 56-57 26.201911879635425 19.533364732186758 22.425250888915343 31.839472499262474 58-59 25.234068101007693 19.701314093772158 24.523453390817387 30.541164414402754 60-61 26.961436342275107 21.45145512983081 21.79123557938648 29.795872948507608 62-63 24.2861505178223 25.57229586000942 19.989338191529555 30.152215430638723 64-65 24.019087742543206 26.68014057025148 19.306671911310318 29.994099775894995 66-67 24.264412850067025 26.639253052330847 18.95162333797415 30.144710759627973 68-69 23.90315351451507 27.24713140420158 19.26630195690766 29.58341312437569 70-71 24.185225631815666 26.47544419888931 19.442791116539777 29.89653905275525 72-73 24.086888563398944 24.939056895757535 20.204385833251386 30.76966870759214 74-75 23.555609612189656 25.126932452785265 20.85806855646359 30.459389378561486 76 23.893319807673397 25.23122150165879 20.440653579210508 30.4348051114573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 4.5 17 8.0 18 9.0 19 9.5 20 20.0 21 31.0 22 33.0 23 49.5 24 83.0 25 102.0 26 152.0 27 246.0 28 339.0 29 388.0 30 481.0 31 643.0 32 854.0 33 996.0 34 1188.0 35 1549.0 36 1845.5 37 1973.0 38 2366.5 39 4663.0 40 7511.0 41 8169.5 42 7883.0 43 7164.5 44 6092.0 45 5492.5 46 5247.0 47 5128.5 48 4806.0 49 4297.0 50 3992.0 51 3765.5 52 3345.0 53 3088.0 54 3025.0 55 3079.5 56 3222.5 57 3519.5 58 3728.0 59 4036.0 60 4777.5 61 5542.0 62 5873.0 63 6176.0 64 6807.5 65 7595.0 66 8256.0 67 8458.0 68 8772.5 69 9030.5 70 9063.5 71 9153.0 72 8864.5 73 8185.5 74 7092.5 75 6390.0 76 5667.5 77 4153.0 78 2748.5 79 2136.0 80 1698.0 81 921.0 82 392.0 83 202.0 84 147.5 85 54.5 86 9.0 87 2.0 88 2.5 89 2.0 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 5.175635179827444E-4 5 0.0 6 5.175635179827444E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 193213.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.46992482723786 #Duplication Level Percentage of deduplicated Percentage of total 1 65.59737610963073 37.04278897774896 2 22.48499658780049 25.394521341075873 3 6.540133409663189 11.079625260113614 4 2.2064196291161484 4.983854023741237 5 1.0327022547729647 2.915830934797418 6 0.597094488887042 2.023072854132556 7 0.40267824439285166 1.5917447133299918 8 0.2757237966039772 1.2456081653845774 9 0.20530489415888395 1.043419674583458 >10 0.639717990135956 5.027791769693276 >50 0.008926297418870511 0.33044414788357684 >100 0.004959054121594727 0.62952410545956 >500 0.0 0.0 >1k 0.0029754324729568365 2.906228276894712 >5k 9.918108243189454E-4 3.7855457551611793 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 6759 3.498211818045369 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 1881 0.9735369773255422 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 1662 0.8601905668873212 No Hit CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG 1646 0.8519095505995974 Illumina PCR Primer Index 9 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 410 0.21220104237292522 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 241 0.12473280783384141 Illumina PCR Primer Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT 239 0.12369768079787594 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0010351270359654889 0.0 2 0.0 0.0 0.0 0.0020702540719309778 0.0 3 0.0 0.0 0.0 0.0020702540719309778 0.0 4 0.0 0.0 0.0 0.011386397395620378 0.0 5 0.0 0.0 0.0 0.012939087949568611 0.0 6 0.0 0.0 0.0 0.012939087949568611 0.0 7 0.0 0.0 0.0 0.013456651467551355 0.0 8 0.0 0.0 0.0 0.0139742149855341 0.0 9 0.0 0.0 0.0 0.01966741368334429 0.0 10 0.0 0.0 0.0 0.022772794791240756 0.0 11 0.0 0.0 0.0 0.023290358309223498 0.0 12 0.0 0.0 0.0 0.025360612381154477 0.0 13 0.0 0.0 0.0 0.025878175899137223 0.0 14 0.0 0.0 0.0 0.02898355700703369 0.0 15 0.0 0.0 0.0 0.030536247560981923 0.0 16 0.0 0.0 0.0 0.03157137459694741 0.0 17 0.0 0.0 0.0 0.03519431922282662 0.0 18 0.0 0.0 0.0 0.035711882740809366 0.0 19 0.0 0.0 0.0 0.03778213681274034 0.0 20 0.0 0.0 0.0 0.039852390884671324 0.0 21 0.0 0.0 0.0 0.04451046254651602 0.0 22 0.0 0.0 0.0 0.04813340717239523 0.0 23 0.0 0.0 0.0 0.0512387882802917 0.0 24 0.0 0.0 0.0 0.05434416938818817 0.0 25 0.0 0.0 0.0 0.059002241050032864 0.0 26 0.0 0.0 0.0 0.07711696417942893 0.0 27 0.0 0.0 0.0 0.15216367428692687 0.0 28 0.0 0.0 0.0 0.24273728993390714 0.0 29 0.0 0.0 0.0 0.38040918571731713 0.0 30 0.0 0.0 0.0 0.5196337720546754 0.0 31 0.0 0.0 0.0 0.6666218111617749 0.0 32 0.0 0.0 0.0 0.8296543193263394 0.0 33 0.0 0.0 0.0 1.0320216548575925 0.0 34 0.0 0.0 0.0 1.3171991532660845 0.0 35 0.0 0.0 0.0 1.715205498594815 0.0 36 0.0 0.0 0.0 2.2736565344981963 0.0 37 0.0 0.0 0.0 3.0329222153788824 0.0 38 0.0 0.0 0.0 3.8310051601082744 0.0 39 0.0 0.0 0.0 4.596999166722736 0.0 40 0.0 0.0 0.0 5.416819779207403 0.0 41 0.0 0.0 0.0 6.238710645764002 0.0 42 0.0 0.0 0.0 7.027477447169704 0.0 43 0.0 0.0 0.0 7.803822724143821 0.0 44 0.0 0.0 0.0 8.617432574412694 0.0 45 0.0 0.0 0.0 9.44656933022105 0.0 46 0.0 0.0 0.0 10.31400578636013 0.0 47 0.0 0.0 0.0 11.148318177348315 0.0 48 0.0 0.0 0.0 11.883775936401795 0.0 49 0.0 0.0 0.0 12.5824866856785 5.175635179827444E-4 50 0.0 0.0 0.0 13.26308271182581 5.175635179827444E-4 51 0.0 0.0 0.0 13.977320366641996 5.175635179827444E-4 52 0.0 0.0 0.0 14.715883506803372 5.175635179827444E-4 53 0.0 0.0 0.0 15.429603598101577 5.175635179827444E-4 54 0.0 0.0 0.0 16.09001464704756 5.175635179827444E-4 55 0.0 0.0 0.0 16.788207832806282 5.175635179827444E-4 56 0.0 0.0 0.0 17.492094217262814 5.175635179827444E-4 57 0.0 0.0 0.0 18.15871602842459 5.175635179827444E-4 58 0.0 0.0 0.0 18.846557943823655 5.175635179827444E-4 59 0.0 0.0 0.0 19.526118842935 5.175635179827444E-4 60 0.0 0.0 0.0 20.170485422823518 5.175635179827444E-4 61 0.0 0.0 0.0 20.814852002712033 0.0010351270359654889 62 0.0 0.0 0.0 21.453007820384755 0.0010351270359654889 63 0.0 0.0 0.0 22.087540693431603 0.0010351270359654889 64 0.0 0.0 0.0 22.709134478528878 0.0010351270359654889 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATTG 15 0.0022220965 70.0 57 CAGCTTA 15 0.0022220965 70.0 11 TCTTTAT 55 3.6379788E-11 57.272724 1 CTAAGTC 20 0.00694333 52.5 26 ATTGGGG 20 0.00694333 52.5 60 CTTTATA 60 8.367351E-11 52.5 2 TTTATAC 75 1.4551915E-11 46.666668 3 CAGCTAG 30 5.8672007E-4 46.666664 19 TGCTACA 30 5.8672007E-4 46.666664 22 GGATTGT 30 5.8672007E-4 46.666664 13 AGAGATA 30 5.8672007E-4 46.666664 33 GTCTACA 35 0.001253178 40.0 1 TCTCGTA 1395 0.0 35.62724 38 CGTATGC 1435 0.0 35.609753 41 TATGCCG 1435 0.0 35.609753 43 TCGTATG 1435 0.0 35.609753 40 CTCGTAT 1430 0.0 35.48951 39 CTTGAAA 1425 0.0 35.368423 57 TCTGCTT 1425 0.0 35.368423 53 GTATGCC 1445 0.0 35.36332 42 >>END_MODULE