FastQCFastQC Report
Wed 25 May 2016
SRR1780179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787723
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGTA250.002364237635.163692
AATCGGA401.7997783E-533.05198717
TAATCGG353.187288E-431.4780816
AACGCAA300.005694473629.38515321
TGGCAAC300.005753182329.3235529
TTGGCAA407.043656E-427.4908328
ATACACG450.001407251824.4161284
ATACAGT602.8941437E-421.9745146
AATCTAA654.8779996E-420.33968419
ATATCGC550.00446126520.01621834
GGTACAT550.004509099319.9793661
GGTAATA708.1764325E-418.8376881
GTACAAG708.1764325E-418.8376881
TTCGGTA600.00742413218.32721926
GCTATAA600.00745410818.314421
CATATAG600.00745956918.3120963
ATTGTCT600.00745956918.3120968
TTGTATA902.2316622E-417.0934582
CTTTGGC800.001991956316.49449726
TAATACA800.002002978716.4808864
Produced by FastQC (version 0.11.5)