FastQCFastQC Report
Wed 25 May 2016
SRR1780179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787723
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT150341.9085389153293733No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC39460.5009375122981048No Hit
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG38510.488877435342119RNA PCR Primer, Index 36 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG34920.44330303926634107No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC9710.12326668130802326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG5750.034.4347344
TCTTTAT1650.032.0569341
CCGTCTT6350.031.52751441
GTCTTCT6400.031.28120643
CTTTATA1650.030.668572
AATTATC300.005745636829.33142930
ATGCCGT23350.028.35970344
CGTCTTC7400.027.05401442
CTATAAC609.742656E-625.6682592
GCCGTCT8050.025.41611340
TATGCCG26150.025.15483943
TTAATAC551.5922613E-424.0014883
CTAATTC551.5936332E-423.99844228
CTGTTAT651.8132623E-523.69076714
TTTATAC2350.022.4694793
CCATACA703.211981E-522.0013642
GTTATCC602.8728085E-421.99857116
AATCTCG30650.021.03096636
ACACATC31650.020.8517276
CTCGTAT31150.020.8346439
Produced by FastQC (version 0.11.5)