FastQCFastQC Report
Wed 25 May 2016
SRR1780178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343870
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACC150.002223574870.0000149
CAAGCTT150.002223574870.0000121
CACTAGG200.006947914652.566
CCTTTGC200.006947914652.543
AAAGCGA200.006947914652.51
AGACAGG503.4737495E-642.0000048
ACTAGGA350.001254541840.067
GATGCAA501.707996E-435.00000470
TAAGAGA952.814886E-729.4736864
AGAGACA952.814886E-729.4736866
AAGAGAC1004.4094486E-728.0000025
GAGACAG1004.4094486E-728.0000027
TATAAGA1056.753471E-726.6666662
GTATAAG1101.0134372E-625.4545441
TCAGACG700.001224806525.08
ATAAGAG1151.4927355E-624.3478283
CTAGGCC1205.7027763E-520.4166684
AAGTGTA5950.020.070
TGAGGTT900.005269496219.44444348
TCTCACG950.00720058318.42105311
Produced by FastQC (version 0.11.5)