##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780178_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 343870 Sequences flagged as poor quality 0 Sequence length 76 %GC 63 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.244132957222206 34.0 32.0 34.0 21.0 34.0 2 31.384441794864337 34.0 33.0 34.0 23.0 34.0 3 31.020199493994824 34.0 33.0 34.0 21.0 34.0 4 30.83045918515718 34.0 32.0 34.0 21.0 34.0 5 30.777232675138862 34.0 32.0 34.0 21.0 34.0 6 34.11517433913979 38.0 34.0 38.0 23.0 38.0 7 34.53437345508477 38.0 35.0 38.0 25.0 38.0 8 34.56445459039753 38.0 35.0 38.0 25.0 38.0 9 34.85529996801117 38.0 36.0 38.0 26.0 38.0 10-11 34.52858783842731 38.0 35.0 38.0 24.5 38.0 12-13 34.60520109343648 38.0 35.0 38.0 25.0 38.0 14-15 34.5876508564283 38.0 35.0 38.0 25.0 38.0 16-17 34.696991595661146 38.0 35.0 38.0 25.0 38.0 18-19 34.694880332683866 38.0 35.5 38.0 25.5 38.0 20-21 34.73874574693925 38.0 35.5 38.0 25.5 38.0 22-23 34.77613051443859 38.0 36.0 38.0 26.0 38.0 24-25 34.74154622386367 38.0 35.5 38.0 25.5 38.0 26-27 34.712808619536446 38.0 35.5 38.0 25.0 38.0 28-29 34.82154011690464 38.0 36.0 38.0 26.0 38.0 30-31 34.81359670805828 38.0 36.0 38.0 26.0 38.0 32-33 34.923072672812395 38.0 36.0 38.0 26.5 38.0 34-35 34.82265245586995 38.0 36.0 38.0 25.5 38.0 36-37 34.72021548841132 38.0 35.5 38.0 25.0 38.0 38-39 34.717711635210975 38.0 35.5 38.0 25.5 38.0 40-41 34.814893709832205 38.0 36.0 38.0 25.5 38.0 42-43 34.8437345508477 38.0 36.0 38.0 26.0 38.0 44-45 34.962927850641236 38.0 36.0 38.0 27.0 38.0 46-47 34.943567336493444 38.0 36.0 38.0 26.5 38.0 48-49 34.934805304330126 38.0 36.0 38.0 27.0 38.0 50-51 34.848012330241076 38.0 36.0 38.0 26.5 38.0 52-53 34.880835780963736 38.0 36.0 38.0 26.5 38.0 54-55 34.879493704016056 38.0 36.0 38.0 26.5 38.0 56-57 34.84273998894931 38.0 36.0 38.0 26.5 38.0 58-59 34.92121295838544 38.0 36.0 38.0 27.0 38.0 60-61 34.910916916276506 38.0 36.0 38.0 27.0 38.0 62-63 34.752892081309795 38.0 36.0 38.0 25.0 38.0 64-65 34.726728124000346 38.0 36.0 38.0 25.5 38.0 66-67 34.56506674033792 38.0 35.0 38.0 25.0 38.0 68-69 34.47336348038503 38.0 35.0 38.0 25.0 38.0 70-71 34.40945124611045 38.0 35.0 38.0 25.0 38.0 72-73 34.42508506121499 38.0 35.0 38.0 25.0 38.0 74-75 34.416951173408556 38.0 35.0 38.0 25.0 38.0 76 31.920074446738592 36.0 29.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10507312457536955 1101 2 0.6894164326885637 1101 3 0.7024131211125884 1101 4 0.744277737418173 1101 5 -0.02166797517387309 1101 6 0.5575732992463358 1101 7 -0.10860373554729819 1101 8 -0.18142362949046742 1101 9 -0.2033919288817927 1101 10-11 -0.1395922996154937 1101 12-13 0.20193470763813792 1101 14-15 0.20832197467844082 1101 16-17 -0.03803277655603665 1101 18-19 0.073891396019377 1101 20-21 0.5454890962501722 1101 22-23 0.04981908065093421 1101 24-25 -0.04761534980978155 1101 26-27 -0.17225696824496595 1101 28-29 -0.302883303562659 1101 30-31 -0.43724310757433926 1101 32-33 -0.1999813426571606 1101 34-35 -0.0069882509090746225 1101 36-37 0.08778818376649866 1101 38-39 0.12260221174071262 1101 40-41 0.4419920633559542 1101 42-43 -0.1365504023366384 1101 44-45 0.2916070958379535 1101 46-47 0.5578781553038752 1101 48-49 -0.06478176577603278 1101 50-51 0.38584613681864965 1101 52-53 0.34125967113216404 1101 54-55 0.321483148359583 1101 56-57 0.5000672812129565 1101 58-59 0.42241171901385854 1101 60-61 0.4083134742978132 1101 62-63 0.2698098932477677 1101 64-65 0.22634656214779625 1101 66-67 -0.13161443892524005 1101 68-69 -0.12732605988309587 1101 70-71 0.24891116970798066 1101 72-73 0.21116935157184002 1101 74-75 0.02049977366498723 1101 76 -0.9640266499456303 1102 1 0.14036074811665245 1102 2 0.26280062156677886 1102 3 0.47265719148893126 1102 4 0.5307614569999721 1102 5 -0.008236856653876856 1102 6 0.09353155179665862 1102 7 -0.31663885124064706 1102 8 -0.1639745001349766 1102 9 0.4059912815405724 1102 10-11 0.24858741010926622 1102 12-13 0.4136163833343858 1102 14-15 0.26341221005142756 1102 16-17 0.5379923371441606 1102 18-19 0.49532048514944904 1102 20-21 0.3901864091204885 1102 22-23 0.29658700541854444 1102 24-25 0.19710053560216778 1102 26-27 -0.02039020131677205 1102 28-29 0.22187480040733476 1102 30-31 0.2897948891278972 1102 32-33 0.4241104147582604 1102 34-35 0.19183760206887257 1102 36-37 0.2350457894487903 1102 38-39 0.1708794952573598 1102 40-41 0.08574127062551895 1102 42-43 0.15326505001799973 1102 44-45 0.21544155560348344 1102 46-47 -0.3302313411537483 1102 48-49 -0.00877422804206418 1102 50-51 0.0737866684463313 1102 52-53 0.3927848844898989 1102 54-55 -0.016872321027435078 1102 56-57 0.2135020236435139 1102 58-59 0.19385620976382967 1102 60-61 0.45809015080943283 1102 62-63 -0.08362330902080117 1102 64-65 0.14853667249586522 1102 66-67 0.12824486274718794 1102 68-69 0.3245959716811697 1102 70-71 0.25955414203941984 1102 72-73 0.3893932730054601 1102 74-75 0.23242153343596073 1102 76 0.3215012632254748 1103 1 0.2752735080970652 1103 2 0.23464413075468826 1103 3 0.6238624868850984 1103 4 0.6408123147957347 1103 5 0.4011533652476196 1103 6 -0.14391937300768376 1103 7 0.2574829497438671 1103 8 0.35897164386474856 1103 9 0.16950038353966335 1103 10-11 -0.1913464833470897 1103 12-13 0.41361208791244763 1103 14-15 0.046407493678145784 1103 16-17 0.35384115547358164 1103 18-19 0.22069268346049142 1103 20-21 0.3190918067477 1103 22-23 0.08939840203203175 1103 24-25 0.10874907558825697 1103 26-27 0.08690836487138398 1103 28-29 0.24409609195480186 1103 30-31 -0.2057206903674782 1103 32-33 0.20860265293081426 1103 34-35 0.09298168770239812 1103 36-37 0.25173350368296354 1103 38-39 -0.48359246852363924 1103 40-41 0.26250862198244107 1103 42-43 0.11345185791813606 1103 44-45 0.2756655189034021 1103 46-47 0.19754607867219676 1103 48-49 0.18658907874556263 1103 50-51 -0.5258875237995682 1103 52-53 0.21217313049730535 1103 54-55 0.26194351705234453 1103 56-57 0.5560876958340941 1103 58-59 -0.11482032778245355 1103 60-61 0.23427611680687477 1103 62-63 0.23593997543062528 1103 64-65 0.31959111267352114 1103 66-67 0.28164941417627887 1103 68-69 0.2768673908026713 1103 70-71 0.5155172615466554 1103 72-73 0.7071664403636859 1103 74-75 0.213059919044575 1103 76 0.5632862687665714 1104 1 0.12258509610118296 1104 2 0.0654111681085503 1104 3 0.35112225306701816 1104 4 0.6733497244484781 1104 5 -0.30669181395918343 1104 6 0.21910612878376412 1104 7 0.1987618825614561 1104 8 0.004841395045616537 1104 9 0.20483964290912127 1104 10-11 0.37878034374406866 1104 12-13 -0.09544433340640524 1104 14-15 0.1562220261039755 1104 16-17 0.09446859624421933 1104 18-19 0.30767709972113977 1104 20-21 -0.340223103803865 1104 22-23 0.14764526234866793 1104 24-25 0.1850219955867587 1104 26-27 0.18805080049702383 1104 28-29 0.1548584182339212 1104 30-31 0.38890372539726314 1104 32-33 0.08336245010665522 1104 34-35 0.07916150198687433 1104 36-37 0.22934350107947665 1104 38-39 0.16122039023089485 1104 40-41 -0.26666594813198685 1104 42-43 0.19844336558603004 1104 44-45 0.14992321469194536 1104 46-47 0.10622251462017829 1104 48-49 -0.11935493385036722 1104 50-51 0.20111189351486303 1104 52-53 0.10042147852175276 1104 54-55 -0.05041933488354289 1104 56-57 0.1050363823120648 1104 58-59 0.012769213631614207 1104 60-61 0.1253728054172356 1104 62-63 0.20705984748775563 1104 64-65 0.46593507072016394 1104 66-67 0.346395698006134 1104 68-69 0.2262822257290864 1104 70-71 0.2535133191209411 1104 72-73 0.31944188946548735 1104 74-75 0.2934364330972201 1104 76 0.5767128317895889 1105 1 0.4709098533145841 1105 2 0.5378332963291719 1105 3 0.7509922378680578 1105 4 1.0691178212823331 1105 5 0.9367204440935168 1105 6 0.9190060231845649 1105 7 0.5626823596500969 1105 8 0.08364098241521845 1105 9 0.6120802076511964 1105 10-11 0.4819745669185238 1105 12-13 -0.46919225041642676 1105 14-15 0.4266887379402391 1105 16-17 0.23899605508799482 1105 18-19 0.6135988952737677 1105 20-21 0.6022070206566923 1105 22-23 0.5007402955239115 1105 24-25 0.5077999870045389 1105 26-27 0.46129025431110904 1105 28-29 0.14971274627509246 1105 30-31 0.6807954770621336 1105 32-33 0.20924371509960338 1105 34-35 0.4635441129118192 1105 36-37 0.30336670848728176 1105 38-39 0.5336528952514357 1105 40-41 0.45800092607067455 1105 42-43 0.7249713493738099 1105 44-45 0.46289207818819733 1105 46-47 0.5877914867676282 1105 48-49 0.4753013193923863 1105 50-51 0.3432313243779106 1105 52-53 0.18839842981793709 1105 54-55 0.25515300667188257 1105 56-57 0.200220631550593 1105 58-59 0.3263301651231316 1105 60-61 0.30876595795009365 1105 62-63 0.4891943552306799 1105 64-65 -0.13125129050234818 1105 66-67 0.516476026645492 1105 68-69 0.45543248789588375 1105 70-71 0.5265910169363224 1105 72-73 0.4561470723718415 1105 74-75 0.04462518708771057 1105 76 0.31466811388594707 1106 1 0.13249211133899763 1106 2 -0.034955172381202715 1106 3 0.585099420683008 1106 4 0.7960981300762455 1106 5 0.10528910225716359 1106 6 0.7898974729247499 1106 7 -0.498532552957748 1106 8 -0.05049914338970751 1106 9 0.24389567718203864 1106 10-11 -0.296393698415514 1106 12-13 0.32465145063526535 1106 14-15 0.20165859199657632 1106 16-17 0.41919110245131463 1106 18-19 0.2383036514956416 1106 20-21 0.17395281265589801 1106 22-23 0.11676799249812575 1106 24-25 0.4176542919696047 1106 26-27 0.3932319017092425 1106 28-29 0.2743724389729323 1106 30-31 0.27030251318753784 1106 32-33 0.3872151106392465 1106 34-35 0.19697161347122005 1106 36-37 0.38819956943057576 1106 38-39 0.38663237114711535 1106 40-41 0.5289044963449072 1106 42-43 0.41180627391570823 1106 44-45 0.30463208962500943 1106 46-47 0.21634373559286502 1106 48-49 0.39133709672263706 1106 50-51 0.3579810819672318 1106 52-53 0.6329174621642153 1106 54-55 0.3986368210971847 1106 56-57 0.4273003908803048 1106 58-59 0.6742267862911717 1106 60-61 0.45601335358918504 1106 62-63 0.2801788533784162 1106 64-65 0.5224044490366992 1106 66-67 0.02294808104493029 1106 68-69 0.17158978134955305 1106 70-71 0.22358232744576156 1106 72-73 0.4022883915832054 1106 74-75 0.3507821076018658 1106 76 0.8902443054276823 1107 1 0.2784815506148348 1107 2 0.03228772427456761 1107 3 0.2872890946416007 1107 4 -0.1641587637298052 1107 5 -0.1521411215296098 1107 6 -0.46743007715487295 1107 7 0.132644632525448 1107 8 -0.09748179362858167 1107 9 0.5942896089564087 1107 10-11 0.08629006656646965 1107 12-13 0.4471087048469826 1107 14-15 -0.04986579824732473 1107 16-17 0.15482726035928351 1107 18-19 0.2990613415467678 1107 20-21 0.2679883084232273 1107 22-23 0.11624414705348585 1107 24-25 0.5903556581585221 1107 26-27 0.1367760304494965 1107 28-29 0.4392947003089489 1107 30-31 -0.05505474940570565 1107 32-33 0.2323150603500821 1107 34-35 0.31401859193069726 1107 36-37 0.2266550635215978 1107 38-39 0.057828205528139165 1107 40-41 0.4999389863289849 1107 42-43 0.11576130702274412 1107 44-45 0.1855745923490062 1107 46-47 -0.03899529717890715 1107 48-49 0.3836617132677773 1107 50-51 0.25589408171578043 1107 52-53 0.2763798710151164 1107 54-55 0.19086819315317172 1107 56-57 0.3622785989098034 1107 58-59 0.02628445119771783 1107 60-61 0.05281894406777354 1107 62-63 0.1958554521547171 1107 64-65 0.31834674222252346 1107 66-67 0.1685194916559425 1107 68-69 0.5098357372627191 1107 70-71 0.4412872557837062 1107 72-73 0.38671970496850605 1107 74-75 0.40086487518212266 1107 76 0.052112547821444366 1108 1 -0.37324676957922165 1108 2 0.11594665176308894 1108 3 0.13926864935008254 1108 4 0.521460226134181 1108 5 0.780811750340785 1108 6 0.7986424343239449 1108 7 0.5367120326813861 1108 8 0.08813069879106905 1108 9 0.1168119168486399 1108 10-11 0.5672395597658095 1108 12-13 0.048621526470476795 1108 14-15 0.6864787395678036 1108 16-17 0.3645052037015688 1108 18-19 0.41416789963391665 1108 20-21 0.19592623617164406 1108 22-23 0.4109411495658293 1108 24-25 0.01546719761967097 1108 26-27 0.5390609650418838 1108 28-29 0.33185945817086093 1108 30-31 0.46890024496317295 1108 32-33 0.23722279664047363 1108 34-35 0.3357124756697587 1108 36-37 -0.06298151275839814 1108 38-39 0.5341352747475767 1108 40-41 0.3976702953474387 1108 42-43 0.28273696326461817 1108 44-45 0.1507796950086302 1108 46-47 0.4617876448638967 1108 48-49 0.35958110164167323 1108 50-51 0.3623492926661456 1108 52-53 0.15783291615069572 1108 54-55 0.1622068832683894 1108 56-57 0.2690413555588549 1108 58-59 0.48701714633762805 1108 60-61 0.339105004883848 1108 62-63 0.5227960221971628 1108 64-65 0.5419909421705427 1108 66-67 0.6659205139205255 1108 68-69 0.45288888635740676 1108 70-71 0.6554971665521379 1108 72-73 0.12120705661019571 1108 74-75 0.2741441353397889 1108 76 0.3599614605824222 1109 1 0.2935878443520039 1109 2 0.3944789426237598 1109 3 0.1589904567025755 1109 4 0.3373600335946385 1109 5 -0.045778950179251154 1109 6 0.4989532867162936 1109 7 0.6940411313444059 1109 8 0.12136851211768374 1109 9 0.25583401391016025 1109 10-11 0.5196175130884626 1109 12-13 0.2273875189488379 1109 14-15 0.6915688912601681 1109 16-17 0.4066760870441968 1109 18-19 0.662190770452483 1109 20-21 0.2554549191651816 1109 22-23 0.5349840750872161 1109 24-25 0.18929823967246762 1109 26-27 0.40867165728452903 1109 28-29 0.04094705466555837 1109 30-31 0.317654361170689 1109 32-33 0.1400713022097264 1109 34-35 0.5456086932574351 1109 36-37 0.2834344626253298 1109 38-39 0.5839402609818976 1109 40-41 0.13407219015246596 1109 42-43 0.5375189617171046 1109 44-45 0.38123895580073963 1109 46-47 0.5466478737520291 1109 48-49 0.47536462061672324 1109 50-51 0.651708558856555 1109 52-53 0.57593725638786 1109 54-55 0.6891860368141778 1109 56-57 0.4864502141772462 1109 58-59 0.48959821088336497 1109 60-61 0.6321429909562184 1109 62-63 0.687819954682368 1109 64-65 0.6061610427589557 1109 66-67 0.8677181494629593 1109 68-69 0.5436400155028593 1109 70-71 0.6177922558940807 1109 72-73 0.22340484150573303 1109 74-75 0.6896482920345903 1109 76 0.11757756912433948 1110 1 -0.15631974980865948 1110 2 0.01694999916013984 1110 3 0.6635285607835364 1110 4 0.10545025368046979 1110 5 0.32578803961168035 1110 6 0.4607514688091072 1110 7 0.023610111414953394 1110 8 0.35366035463680845 1110 9 0.5076510325779182 1110 10-11 0.1360355428893527 1110 12-13 0.5329109891435166 1110 14-15 0.08654499013442063 1110 16-17 0.17595914212341057 1110 18-19 -0.015052958833948082 1110 20-21 -0.15182603901229186 1110 22-23 0.03465754483763561 1110 24-25 0.3345486683822543 1110 26-27 0.0698587613388284 1110 28-29 0.1949038460504866 1110 30-31 -0.02763249676421964 1110 32-33 -0.01831697113385644 1110 34-35 0.05387079708961551 1110 36-37 0.27083417119153097 1110 38-39 0.15324464048557473 1110 40-41 0.3383346316213647 1110 42-43 0.2860451330055511 1110 44-45 0.22788968838356993 1110 46-47 0.2828391982653429 1110 48-49 0.3740058549454872 1110 50-51 -0.06988553072379489 1110 52-53 0.3279508595936278 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-0.02989205775681114 2116 9 -0.805730457765442 2116 10-11 0.22767015536171442 2116 12-13 0.01312320305351733 2116 14-15 0.19161838666751407 2116 16-17 0.05951660242164536 2116 18-19 0.03014093118230221 2116 20-21 -0.1322048337745798 2116 22-23 -0.13743057138532322 2116 24-25 -0.6250731426040019 2116 26-27 -0.2079564550648456 2116 28-29 -0.39499807316284574 2116 30-31 -0.04179691711333078 2116 32-33 -0.0590825868267828 2116 34-35 -0.22771015615668944 2116 36-37 -0.36050599700465114 2116 38-39 0.05179690144204585 2116 40-41 -0.5946304570703163 2116 42-43 -0.43964542034575516 2116 44-45 -0.20307516810450466 2116 46-47 -0.16008793018364287 2116 48-49 -0.2261559303974181 2116 50-51 0.11059220296894523 2116 52-53 -0.2587506880939259 2116 54-55 -0.13045269881261845 2116 56-57 -0.2586158752166625 2116 58-59 -0.3269600868914253 2116 60-61 -0.4445748904319444 2116 62-63 -0.10499487518368511 2116 64-65 -0.7534582594078998 2116 66-67 -0.6003494977337738 2116 68-69 -0.383418081685285 2116 70-71 -0.34870195835330264 2116 72-73 -0.42908660099883633 2116 74-75 -0.4045180229541856 2116 76 -0.2503556589661251 2117 1 -0.17795560417956224 2117 2 -0.5425560843376225 2117 3 -0.14700445462174372 2117 4 -0.5323655701818346 2117 5 -0.5644786242783226 2117 6 -0.3499702729923584 2117 7 -0.188330933061998 2117 8 -0.726532745005521 2117 9 -0.6321613863998863 2117 10-11 -0.1306374580425711 2117 12-13 -0.8042675902803609 2117 14-15 -0.03167257365510068 2117 16-17 -0.8295503670838329 2117 18-19 -0.3450143805554404 2117 20-21 -1.207289472947636 2117 22-23 -0.3526727966400003 2117 24-25 -0.4594024553858205 2117 26-27 -0.23658800219870812 2117 28-29 -0.9389051211866075 2117 30-31 -0.404016707263132 2117 32-33 -0.6545325253126464 2117 34-35 -0.2911399277759301 2117 36-37 -0.3892298916582675 2117 38-39 -0.4782885467258069 2117 40-41 -0.265552741671641 2117 42-43 -0.2819647015920381 2117 44-45 -0.49155355573207515 2117 46-47 -0.5304251436392136 2117 48-49 -0.48675376229135026 2117 50-51 -0.43177164931164924 2117 52-53 -0.719103837220672 2117 54-55 -0.3400395924862778 2117 56-57 -0.8100900774409467 2117 58-59 -0.3536240976129008 2117 60-61 -0.770499815714409 2117 62-63 -0.2596510218651318 2117 64-65 -0.7133481445306415 2117 66-67 -0.556215015322536 2117 68-69 -0.8904792325338136 2117 70-71 -0.3138350456300998 2117 72-73 -2.6333814475493185 2117 74-75 -0.9043699616415353 2117 76 -1.1485367944284874 2118 1 -0.2636953732631184 2118 2 -0.48324908713853176 2118 3 0.03764315066576174 2118 4 -0.670734833707936 2118 5 -0.2058404863114518 2118 6 -0.2871843206204545 2118 7 -0.20015241006540663 2118 8 -0.1908354331162414 2118 9 -0.19284982663663897 2118 10-11 -1.3379679777946691 2118 12-13 -0.45461992162391596 2118 14-15 -0.5145122628937884 2118 16-17 -0.3066496218650201 2118 18-19 -0.304579447789898 2118 20-21 -0.06593077936184244 2118 22-23 -0.09116499592266791 2118 24-25 -0.26579606526123456 2118 26-27 -0.4464453586997337 2118 28-29 -0.4069838064077018 2118 30-31 -0.17049263340029341 2118 32-33 -0.1257345074932985 2118 34-35 -0.5145336833999323 2118 36-37 -0.33294480110404123 2118 38-39 -0.5318820412697391 2118 40-41 -0.31340580175807276 2118 42-43 -0.26590067360830716 2118 44-45 -0.2563589180123387 2118 46-47 -0.6940405955231697 2118 48-49 -0.2695091708309505 2118 50-51 -0.28538859036585507 2118 52-53 -0.07754077878182386 2118 54-55 -0.2920043466781266 2118 56-57 -0.22986578305416572 2118 58-59 -0.5496892028687199 2118 60-61 -0.4976236259624258 2118 62-63 -0.23266297958537052 2118 64-65 -0.43418892371520457 2118 66-67 -0.4048820495290997 2118 68-69 -0.4789399057654933 2118 70-71 -0.2327338834436219 2118 72-73 -0.536520428323179 2118 74-75 -0.8168392277023955 2118 76 -0.6248764139613314 2119 1 -0.5957052308753887 2119 2 -0.7691806923472448 2119 3 -1.7823337265400312 2119 4 -1.2497478554920427 2119 5 -0.36312112401377306 2119 6 -1.1849572304415616 2119 7 -0.5666791229776607 2119 8 -0.8473858773172864 2119 9 -0.8432088384414271 2119 10-11 -0.7344630489885304 2119 12-13 -0.5402655343779657 2119 14-15 -0.8309902962392925 2119 16-17 -0.8076308193823607 2119 18-19 -0.9738702473517833 2119 20-21 -1.2031919452374638 2119 22-23 -1.276177774308941 2119 24-25 -0.828394945631203 2119 26-27 -0.8123179399337559 2119 28-29 -0.7437018908667525 2119 30-31 -1.254738708610219 2119 32-33 -0.887356754055304 2119 34-35 -1.1489425309111851 2119 36-37 -0.9705178204541696 2119 38-39 -1.4681954768301608 2119 40-41 -0.7916521203658391 2119 42-43 -1.048728956436591 2119 44-45 -0.9564243311801661 2119 46-47 -1.2256518891826573 2119 48-49 -0.8762086962051754 2119 50-51 -0.9995710778917157 2119 52-53 -0.8380068856334759 2119 54-55 -1.128608338402607 2119 56-57 -1.1382341048260969 2119 58-59 -0.9451041961752793 2119 60-61 -1.1959624357470275 2119 62-63 -1.4219732509739558 2119 64-65 -1.226144625309459 2119 66-67 -1.8091506390386485 2119 68-69 -1.8665281374802376 2119 70-71 -1.6761895241884162 2119 72-73 -1.087010266463622 2119 74-75 -1.1978861426348502 2119 76 -0.8869702438262479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 32.0 13 366.0 14 1091.0 15 1745.0 16 2106.0 17 2542.0 18 2685.0 19 2540.0 20 2215.0 21 2001.0 22 1823.0 23 1960.0 24 2200.0 25 2561.0 26 3471.0 27 4194.0 28 5079.0 29 6512.0 30 8053.0 31 10169.0 32 12668.0 33 16180.0 34 21739.0 35 30832.0 36 54168.0 37 144937.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.99808067002065 13.379183993951202 7.658417425189752 30.964317910838396 2 27.226568179835404 15.320033733678425 24.63052897897461 32.82286910751156 3 25.195568092593128 15.293861052141796 20.928548579405007 38.58202227586006 4 21.88356064791927 12.393927937883502 20.592084217873033 45.13042719632419 5 25.393608049553613 23.21720417599674 23.335272050484196 28.05391572396545 6 42.90109634454881 21.245238025998198 11.735830401023643 24.11783522842935 7 38.300811353127635 20.01075988018728 12.877540931165848 28.81088783551924 8 31.99028702707418 22.924651757931777 15.306656585337484 29.778404629656556 9 27.43827609270945 12.083636257888156 14.364149242446272 46.113938406956116 10-11 35.991217611306595 15.923604850670312 16.845028644545902 31.240148893477187 12-13 32.71745136243347 13.988425858609357 17.77982958676244 35.51429319219472 14-15 31.255270887253907 14.729258149882224 15.58059731875418 38.43487364410969 16-17 33.89245935964173 16.234478145810918 15.949632128420625 33.92343036612673 18-19 33.25428214150696 15.709570477215227 16.093000261726814 34.94314711955099 20-21 32.6448948730625 16.47701166138366 16.540407712216826 34.33768575333701 22-23 33.714048913833714 15.920260563585076 15.952540204146917 34.41315031843429 24-25 33.59278215604735 16.064210312036526 15.601244656410854 34.74176287550528 26-27 32.50167214354262 16.55567510977986 16.54433361444732 34.398319132230206 28-29 33.321022479425366 16.25890598191177 16.75066158722773 33.669409951435135 30-31 32.77532206938669 15.704335940907901 16.927908802745222 34.592433186960186 32-33 32.109372728065836 16.398202809201152 17.538313897693897 33.954110565039116 34-35 32.23601942594585 16.3000552534388 17.777503126181408 33.68642219443394 36-37 31.980544973391105 15.358711140838107 18.29557681682031 34.36516706895048 38-39 30.719748742257245 15.839125250821532 20.14525838252828 33.29586762439294 40-41 30.228429348300228 15.464419693488818 20.872713525460203 33.43443743275075 42-43 28.81205106580975 15.253147991973712 21.644953034577018 34.28984790763951 44-45 27.146159886003428 15.912990374269345 23.660104109111003 33.280745630616224 46-47 26.823945095530288 16.958589001657604 23.905400296623725 32.31206560618838 48-49 26.132986302963328 17.68938843167476 23.472387820978856 32.70523744438305 50-51 24.935295315090006 19.062727193416116 23.19248553232326 32.809491959170614 52-53 24.578910634832933 19.166836304417366 23.31593334690435 32.938319713845345 54-55 24.52889173234071 18.617791607293455 23.46453601651787 33.388780643847966 56-57 24.500537994009363 19.301480210544682 23.281181841975165 32.916799953470786 58-59 25.179137464739583 19.186320411783523 22.59574839328816 33.038793730188736 60-61 25.500770640067465 18.285543955564602 22.652892081309798 33.56079332305813 62-63 25.40175066158723 18.428039666152905 22.54122197342019 33.62898769883968 64-65 25.89539651612528 18.387762817343763 21.534736964550557 34.182103701980395 66-67 25.87373135196441 19.36022334021578 20.552970599354406 34.213074708465406 68-69 26.009102277023295 19.92787972198796 20.800302439875534 33.26271556111321 70-71 26.44676767383023 20.222613196847647 21.05461366213976 32.276005467182365 72-73 26.302090906447205 20.35972896734231 20.484194608427604 32.85398551778289 74-75 26.053924526600937 21.285255016801433 20.450054453797154 32.210766002800476 76 26.209904905923747 21.597987611597404 19.545176956407946 32.646930526070896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 2.0 17 2.0 18 5.0 19 10.5 20 15.0 21 17.0 22 18.0 23 30.0 24 46.5 25 52.0 26 95.0 27 165.0 28 250.5 29 309.0 30 359.5 31 474.0 32 651.5 33 765.0 34 855.5 35 1133.5 36 1447.5 37 1574.0 38 1886.0 39 2445.0 40 2913.0 41 3315.0 42 3496.0 43 3559.0 44 3852.0 45 4069.0 46 4056.0 47 4046.0 48 3947.5 49 3732.0 50 3605.0 51 3526.0 52 3434.5 53 3669.0 54 3916.0 55 4568.5 56 6175.0 57 8689.5 58 10250.0 59 11866.5 60 15335.0 61 18896.5 62 20606.0 63 21955.5 64 23983.0 65 24954.5 66 25140.5 67 25033.0 68 24458.5 69 23208.5 70 21411.5 71 20290.0 72 18657.5 73 15436.0 74 11893.0 75 9939.0 76 8224.5 77 4997.0 78 2492.5 79 1501.0 80 976.5 81 300.0 82 104.0 83 60.0 84 39.0 85 14.0 86 9.5 87 9.0 88 9.0 89 6.5 90 5.5 91 7.5 92 8.0 93 8.5 94 6.5 95 4.0 96 4.0 97 3.0 98 6.5 99 39.0 100 67.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.4540378631459563E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 343870.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.0068100202893 #Duplication Level Percentage of deduplicated Percentage of total 1 77.26527925901172 57.95422122545292 2 16.325111134380105 24.489890188331163 3 4.351838908392481 9.792526627220939 4 1.2115034869816725 3.634840475478094 5 0.4116054534020197 1.5436606023320165 6 0.17020964194006727 0.7660129365972054 7 0.09554243194022524 0.5016433128991826 8 0.059479563285717345 0.356909784276926 9 0.032300915780441586 0.21805097880824523 >10 0.07674205461889134 0.7216262449888751 >50 3.871502666411981E-4 0.02061762361447509 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002035653008404339 0.0 2 0.0 0.0 0.0 0.0029080757262919124 0.0 3 0.0 0.0 0.0 0.003489690871550295 0.0 4 0.0 0.0 0.0 0.00930584232413412 0.0 5 0.0 0.0 0.0 0.010469072614650885 0.0 6 0.0 0.0 0.0 0.011341495332538459 0.0 7 0.0 0.0 0.0 0.012213918050426034 0.0 8 0.0 0.0 0.0 0.013086340768313607 0.0 9 0.0 0.0 0.0 0.016285224067234712 0.0 10 0.0 0.0 0.0 0.01861168464826824 0.0 11 0.0 0.0 0.0 0.018902492220897432 0.0 12 0.0 0.0 0.0 0.019774914938785006 0.0 13 0.0 0.0 0.0 0.02093814522930177 0.0 14 0.0 0.0 0.0 0.021519760374560153 0.0 15 0.0 2.9080757262919127E-4 0.0 0.02297379823770611 0.0 16 0.0 2.9080757262919127E-4 0.0 0.024137028528222877 0.0 17 0.0 2.9080757262919127E-4 0.0 0.025591066391368832 0.0 18 0.0 2.9080757262919127E-4 0.0 0.029371564835548317 0.0 19 0.0 2.9080757262919127E-4 0.0 0.032570448134469424 5.816151452583825E-4 20 0.0 2.9080757262919127E-4 0.0 0.03606013900601972 5.816151452583825E-4 21 0.0 2.9080757262919127E-4 0.0 0.03867740715968244 5.816151452583825E-4 22 0.0 2.9080757262919127E-4 0.0 0.04478436618489545 5.816151452583825E-4 23 0.0 2.9080757262919127E-4 0.0 0.0500189024922209 5.816151452583825E-4 24 0.0 2.9080757262919127E-4 0.0 0.056416669090063105 5.816151452583825E-4 25 0.0 2.9080757262919127E-4 0.0 0.06514089626893885 5.816151452583825E-4 26 0.0 8.724227178875738E-4 0.0 0.09451246110448716 5.816151452583825E-4 27 0.0 8.724227178875738E-4 0.0 0.3321022479425364 5.816151452583825E-4 28 0.0 8.724227178875738E-4 0.0 0.5536976182859802 5.816151452583825E-4 29 0.0 8.724227178875738E-4 0.0 0.8238578532584988 5.816151452583825E-4 30 0.0 8.724227178875738E-4 0.0 1.111466542588769 5.816151452583825E-4 31 0.0 8.724227178875738E-4 2.9080757262919127E-4 1.4441504056765637 5.816151452583825E-4 32 0.0 8.724227178875738E-4 2.9080757262919127E-4 1.7649111582865618 5.816151452583825E-4 33 0.0 8.724227178875738E-4 2.9080757262919127E-4 2.1746590281210922 5.816151452583825E-4 34 0.0 8.724227178875738E-4 2.9080757262919127E-4 2.732137144851252 5.816151452583825E-4 35 0.0 8.724227178875738E-4 2.9080757262919127E-4 3.4812574519440487 5.816151452583825E-4 36 0.0 8.724227178875738E-4 2.9080757262919127E-4 4.577311193183471 5.816151452583825E-4 37 0.0 8.724227178875738E-4 2.9080757262919127E-4 6.06624596504493 5.816151452583825E-4 38 0.0 8.724227178875738E-4 2.9080757262919127E-4 7.745659696978509 5.816151452583825E-4 39 0.0 8.724227178875738E-4 2.9080757262919127E-4 9.32387239363713 5.816151452583825E-4 40 0.0 8.724227178875738E-4 2.9080757262919127E-4 10.893360863116875 5.816151452583825E-4 41 0.0 8.724227178875738E-4 2.9080757262919127E-4 12.481751824817518 5.816151452583825E-4 42 0.0 8.724227178875738E-4 2.9080757262919127E-4 14.026521650623783 5.816151452583825E-4 43 0.0 8.724227178875738E-4 2.9080757262919127E-4 15.545118794893419 5.816151452583825E-4 44 0.0 8.724227178875738E-4 2.9080757262919127E-4 16.963968941751244 5.816151452583825E-4 45 0.0 8.724227178875738E-4 2.9080757262919127E-4 18.324948381655858 5.816151452583825E-4 46 0.0 8.724227178875738E-4 2.9080757262919127E-4 19.736237531625324 5.816151452583825E-4 47 0.0 8.724227178875738E-4 2.9080757262919127E-4 21.099252624538344 5.816151452583825E-4 48 0.0 8.724227178875738E-4 2.9080757262919127E-4 22.364556373047954 5.816151452583825E-4 49 0.0 8.724227178875738E-4 2.9080757262919127E-4 23.611539244481925 5.816151452583825E-4 50 0.0 8.724227178875738E-4 2.9080757262919127E-4 24.873644109692616 5.816151452583825E-4 51 0.0 8.724227178875738E-4 2.9080757262919127E-4 26.04908831825981 5.816151452583825E-4 52 0.0 8.724227178875738E-4 2.9080757262919127E-4 27.25593974467095 5.816151452583825E-4 53 0.0 8.724227178875738E-4 2.9080757262919127E-4 28.41771599732457 5.816151452583825E-4 54 0.0 8.724227178875738E-4 2.9080757262919127E-4 29.490505132753658 5.816151452583825E-4 55 0.0 8.724227178875738E-4 2.9080757262919127E-4 30.547299851688138 5.816151452583825E-4 56 0.0 8.724227178875738E-4 2.9080757262919127E-4 31.588390961700643 5.816151452583825E-4 57 0.0 8.724227178875738E-4 2.9080757262919127E-4 32.61087038706488 5.816151452583825E-4 58 0.0 8.724227178875738E-4 2.9080757262919127E-4 33.62200831709658 5.816151452583825E-4 59 0.0 8.724227178875738E-4 2.9080757262919127E-4 34.592433186960186 5.816151452583825E-4 60 0.0 0.001163230290516765 2.9080757262919127E-4 35.60909646087184 5.816151452583825E-4 61 0.0 0.001163230290516765 2.9080757262919127E-4 36.58155698374386 5.816151452583825E-4 62 0.0 0.001163230290516765 2.9080757262919127E-4 37.51999302061826 5.816151452583825E-4 63 0.0 0.001163230290516765 2.9080757262919127E-4 38.41917003518772 8.724227178875738E-4 64 0.0 0.001163230290516765 2.9080757262919127E-4 39.35324395847268 8.724227178875738E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACC 15 0.0022235748 70.00001 49 CAAGCTT 15 0.0022235748 70.00001 21 CACTAGG 20 0.0069479146 52.5 66 CCTTTGC 20 0.0069479146 52.5 43 AAAGCGA 20 0.0069479146 52.5 1 AGACAGG 50 3.4737495E-6 42.000004 8 ACTAGGA 35 0.0012545418 40.0 67 GATGCAA 50 1.707996E-4 35.000004 70 TAAGAGA 95 2.814886E-7 29.473686 4 AGAGACA 95 2.814886E-7 29.473686 6 AAGAGAC 100 4.4094486E-7 28.000002 5 GAGACAG 100 4.4094486E-7 28.000002 7 TATAAGA 105 6.753471E-7 26.666666 2 GTATAAG 110 1.0134372E-6 25.454544 1 TCAGACG 70 0.0012248065 25.0 8 ATAAGAG 115 1.4927355E-6 24.347828 3 CTAGGCC 120 5.7027763E-5 20.416668 4 AAGTGTA 595 0.0 20.0 70 TGAGGTT 90 0.0052694962 19.444443 48 TCTCACG 95 0.007200583 18.421053 11 >>END_MODULE